Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Glioblastoma (GBM) is the most frequent and aggressive primary brain cancer. The Src inhibitor, TAT-Cx43, exerts antitumor effects in in vitro and in vivo models of GBM. Because addressing the mechanism of action is essential to translate these results to a clinical setting, in this study we carried out an unbiased proteomic approach. Data-independent acquisition mass spectrometry proteomics allowed the identification of 190 proteins whose abundance was modified by TAT-Cx43. Our results were consistent with the inhibition of Src as the mechanism of action of TAT-Cx43 and unveiled antitumor effectors, such as p120 catenin. Changes in the abundance of several proteins suggested that TAT-Cx43 may also impact the brain microenvironment. Importantly, the proteins whose abundance was reduced by TAT-Cx43 correlated with an improved GBM patient survival in clinical datasets and none of the proteins whose abundance was increased by TAT-Cx43 correlated with shorter survival, supporting its use in clinical trials.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.trsl.2024.06.001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Scalable Acid-Aided Lysis of Skin Samples Improves Proteome Coverage.
J Invest Dermatol
September 2025
LEO Foundation Skin Immunology Research Center, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, DK. Electronic address:
Liquid chromatography-mass spectrometry (LC-MS) is an evolving tool for comprehensive proteomic analyses across tissues. Despite the widespread use of LC-MS in dermatology, full-thickness human skin remains challenging to analyse. The skin extracellular matrix (ECM) presents two major obstacles: the extensive crosslinking complicates protein extraction and the high abundance of ECM proteins can mask lower-abundance proteins, reducing identification numbers.
View Article and Find Full Text PDFThe renoprotective anti-hyperuricemia effect of Cornus officinalis extract in hyperuricemia rats based on network pharmacology and multiple omics.
J Ethnopharmacol
September 2025
Lab of Food Function and Nutrigenomics, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditio
Ethnopharmacological Relevance: Corus officinalis Siebold & Zucc belongs to the genus Cornus in the Cornaceae family, and was first recorded in the "Shennong Herbal Classic", now has been included in "according to the tradition of both food and Chinese herbal medicines", consist of kidney and liver tonifying, antioxidant substances including cycloid glycosides, flavonoids, polyphenols, organic acids, etc. AIM OF THE STUDY: This study was aimed at discovering the mechanism underlying the anti-hyperemia effect of Cor in rats, particularly its protective effect against liver and kidney dysfunction caused by HUA.
Materials And Methods: In this study, the effect of Cor extract against HUA was verified in rats, subsequently, network pharmacology combined with non-targeted metabolomic were performed to investigate its composition characteristics, and further multi-omics studies and molecular validation were performed to reveal molecular mechanism both in vivo and in vitro.
Dissolved organic nitrogen and phosphorus derived from different cyanobacteria regulate distinctly different nitrate reduction pathways in sediments.
Water Res
September 2025
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China. Electronic address:
The differentiation of the nitrate reduction pathway is of great significance in the ecosystem, as it determines the occurrence form of ecosystem N. In order to explore the impact and mechanism of different algal dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP) on differentiation of nitrate (NO-N) reduction pathway, small-scale enclosure experiment was conducted to analyze the DON and DOP composition, nutrient level, microbial community composition and NO-N reduction pathway in ponds with Microcystis and Dolichospermum blooms. The main DON produced by Microcystis included lipids and proteins as well as carbohydrate which were readily degradable, whereas the DOP produced by Dolichospermum predominantly consists of readily degradable forms such as carbohydrate and protein.
View Article and Find Full Text PDFChemoselective Characterization of New Extracellular Matrix Deposition in Bioengineered Tumor Tissues.
Adv Mater
September 2025
Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, 15213, USA.
In both native and engineered tissues, the extracellular matrix (ECM) supports and regulates nearly all aspects of cellular pathophysiology, and in response, cells extensively remodel their surrounding extracellular environments through new ECM protein deposition. Understanding this intricate bi-directional cell-ECM interaction is key to tissue engineering, but it remains challenging to investigate. This is partly due to the limited sensitivity of conventional proteomics to capture low-abundance newly synthesized ECM (newsECM).
View Article and Find Full Text PDFA Distinct Immunological Signature in Late-Onset Myasthenia Gravis: Insights from an Exploratory Proteomics Study.
Ann Neurol
September 2025
Department of Neurology & Rehabilitation Medicine, The George Washington University School of Medicine & Health Sciences, Washington, DC, USA.
Objective: Approximately 80% of patients with myasthenia gravis (MG) have autoantibodies against acetylcholine receptor, and clinical characteristics may vary between early-onset (symptom onset age <50 years) and late-onset MG (EOMG and LOMG), but the pathophysiological differences between EOMG and LOMG are not well established.
Methods: We performed an exploratory in-depth proteomics analysis examining 768 inflammatory proteins utilizing the baseline serum samples from the BeatMG study (NCT02110706). We performed pathway enrichment analysis to assess the pathways involved in LOMG.