Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The regional heterogeneity of microglia was first described a century ago by Pio del Rio Hortega. Currently, new information on microglia heterogeneity throughout central nervous system (CNS) regions is being revealed by high-throughput techniques. It remains unclear whether these spatial specificities translate into different microglial behaviors in vitro. We cultured microglia isolated from the cortex and spinal cord and analyzed the effect of the CNS spatial source on behavior in vitro by applying the same experimental protocol and culture conditions. We analyzed the microglial cell numbers, function, and morphology and found a distinctive in vitro phenotype. We found that microglia were present in higher numbers in the spinal-cord-derived glial cultures, presenting different expressions of inflammatory genes and a lower phagocytosis rate under basal conditions or after activation with LPS and IFN-γ. Morphologically, the cortical microglial cells were more complex and presented longer ramifications, which were also observed in vivo in CX3CR1 transgenic reporter mice. Collectively, our data demonstrated that microglial behavior in vitro is defined according to specific spatial characteristics acquired by the tissue. Thus, our study highlights the importance of microglia as a source of CNS for in vitro studies.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177862 | PMC |
| http://dx.doi.org/10.3390/ijms24097685 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Central nervous system tumors with BCOR internal tandem duplications: a systematic review of clinical, radiological, and pathological features in 69 cases.
J Pathol Transl Med
September 2025
Department of Pathology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Hwasun, Korea.
Central nervous system tumors with BCL6 corepressor (BCOR) internal tandem duplications (ITDs) constitute a rare, recently characterized pediatric neoplasm with distinct molecular and histopathological features. To date, 69 cases have been documented in the literature, including our institutional case. These neoplasms predominantly occur in young children, with the cerebellum representing the most frequent anatomical location.
View Article and Find Full Text PDFPsychological experiences of young end stage renal disease patients awaiting kidney transplantation: A qualitative study.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
May 2025
Nursing Department, Third Xiangya Hospital, Central South University, Changsha 410013.
Objectives: End stage renal disease (ESRD) is a major disease that seriously threatens the health of young people, and kidney transplantation is an effective treatment method to improve its prognosis.Young ESRD patients at a critical stage of life development often face significant physical and psychological challenges while waiting for kidney transplantation. Their psychological state directly affects treatment compliance and transplantation outcomes.
View Article and Find Full Text PDFApplication of stereotactic radiotherapy in the treatment of cardiovascular diseases through sympathectomy.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
May 2025
Department of Cardiovascular Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Sympathectomy, as an emerging treatment method for cardiovascular diseases, has received extensive attention in recent years. Stereotactic radiotherapy (SRT), a precise and noninvasive therapeutic technique, has gradually been introduced into interventions targeting the sympathetic nervous system and has shown promising prospects in the management of cardiovascular conditions. Using three-dimensional imaging, SRT can accurately localize sympathetic ganglia and deliver high-energy radiation to disrupt nerve fibers, thereby achieving effects similar to conventional sympathectomy while reducing surgery-related complications and shortening recovery time.
View Article and Find Full Text PDFCase study exploring the benefits of clean intermittent self-catheterisation for a patient with multiple sclerosis.
Br J Nurs
September 2025
Senior Bladder, Bowel and Stoma Care, Clinical Nurse Specialist and Nurse Prescriber, Hollister Ltd.
The aim of this case study is to illustrate the benefits of clean intermittent self-catheterisation (CISC) in individuals with multiple sclerosis (MS) who have incomplete bladder emptying. People with MS usually start to experience bladder symptoms 6-8 years after diagnosis, although some individuals experience symptoms from the time of diagnosis. MS is a condition of the central nervous system that affects the brain and spinal cord; the immune system attacks myelin, a substance that protects the nerve fibres, preventing messages travelling smoothly along the fibres to control the whole body, which includes the nerves that control the bladder.
View Article and Find Full Text PDFHalf-life prediction of central nervous system (CNS) small molecules in humans using gradient tree boosting.
Future Med Chem
September 2025
Computational Science & Artificial Intelligence, Xenon Pharmaceuticals Inc, Burnaby, BC, Canada.
Aims: To develop a machine learning (ML) model for early-stage prediction of human half-life of oral central nervous system (CNS) drugs and to establish a curated dataset, including key and data, to support future modeling efforts.
Materials & Methods: Human and rat half-life, plasma protein binding (PPB), and liver microsomal clearance (LM) data for 76 diverse CNS drugs and candidates were obtained from public sources or evaluated at WuXi AppTec. Gradient tree boosting (GTB) models were constructed using ChemAxon's Trainer Engine.