Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Hepatic progenitor cells (HPCs) play an important role in the treatment of chronic liver disease.
Objectives: To investigate the effect and mechanism of long noncoding RNAs/small nucleolar RNA host gene 12 (lncRNA SNHG12) on the proliferation and migration of the HPC cell line WB-F344.
Material And Methods: Hepatic progenitor cells were divided into a no-treatment group (sham), empty vector transfection of plasmid pcDNA3.1 (NC vector), pcDNA3.1-SNHG12 (SNHG12), negative short hairpin RNA (sh-NC), SNHG12 shRNA (sh-SNHG12), and pcDNA3.1-SNHG12+salinomycin intervention (SNHG12+salinomycin) groups. Cell proliferation, cell cycle and migration ability, as well as albumin (ALB), alpha-fetoprotein (AFP), â-catenin, cyclin D1, and c-Myc protein expression in each group were determined using Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), flow cytometry, transwell migration assays, enzyme-linked immunosorbent assay (ELISA), and western blot.
Results: The overexpression of lncRNA SNHG12 significantly upregulated proliferation, migration and cell cycle progression of WB-F344 cells. Furthermore, the overexpression of lncRNA SNHG12 increased the level of ALB, and the protein expression of â-catenin, cyclin D1 and c-Myc in the cell line, while decreasing the level of AFP. Conversely, the knockdown of lncRNA SNHG12 displayed the opposite effects. The inhibition of the Wnt/â-catenin signaling pathway with salinomycin significantly downregulated the â-catenin, cyclin D1 and c-Myc protein expression in WB-F344 cells.
Conclusions: The lncRNA SNHG12 promotes the proliferation and migration of WB-F344 cells via activating the Wnt/â-catenin pathway.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.17219/acem/159475 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
FOXO3 Protects Lens Epithelial Cells From UVB-Induced Oxidative Stress via the AMPK/FOXO3 Signaling Pathway.
FASEB J
September 2025
Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China.
Age-related cataract (ARC) represents a major global cause of visual impairment, with ultraviolet B (UVB) radiation recognized as a primary contributor to oxidative damage in the lens. FOXO3, a key regulator of aging, apoptosis, and oxidative stress-induced cell death, was investigated for its role and regulatory mechanisms in UVB-induced oxidative stress using human lens epithelial cells (HLECs). A progressive decrease in FOXO3 protein expression was observed in the lens capsules across various stages of cataract progression, as well as in UVB-exposed animal models and UVB-treated HLECs.
View Article and Find Full Text PDFThe long noncoding RNA SNHG12 defines KEAP1 stability and ferroptosis susceptibility by targeting E3 ligase TRIM25.
J Biol Chem
September 2025
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, China. Electronic address:
Ferroptosis is a novel type of programmed cell death caused by iron-dependent lipid peroxidation. Targeted induction of ferroptosis holds great promise for cancer treatment. SNHG, a newly recognized lncRNA family, has been reported to implicate in the proliferation, invasion, migration or drug resistance of cancer cells.
View Article and Find Full Text PDFComparative Analysis of Differentially Expressed Long Non-Coding RNA in Pre- and Postmenopausal Fibroids.
Int J Mol Sci
July 2025
The Lundquist Institute for Biomedical Innovation, Torrance, CA 90502, USA.
Uterine fibroids (leiomyomas) are benign tumors whose growth is influenced by estrogen and progesterone. This study aimed to compare the profiles of differentially expressed long non-coding RNAs (lncRNAs) in fibroids from postmenopausal and premenopausal women to identify hormone-responsive lncRNAs. RNA sequencing was performed on six pairs of fibroid (Fib) and adjacent myometrium (Myo) tissues from postmenopausal women.
View Article and Find Full Text PDFSubtype-specific expression of lncRNAs SNHG12 and HAGLR as non-invasive biomarkers for diagnosis and prognosis in breast cancer.
Ir J Med Sci
August 2025
Cancer Epidemiology Research Center, Aja University of Medical Sciences, Tehran, Iran.
Background: Long non-coding RNAs (lncRNAs) SNHG12 and HAGLR have different molecular functions in breast cancer (BC). We investigated their expression profiles in different subtypes of BC, breast tissues, and plasma in association with clinicopathological and reproductive conditions.
Methods: The expression levels of SNHG12 and HAGLR were detected by qRT-PCR in 150 BC tumors including 35 triple-negative BC (TNBC), 30 HER2-enriched, 30 luminal B, and 55 luminal A and their corresponding tumor-adjacent apparently normal (TAN), as well as in truly normal (TN) tissues and plasma samples of 150 cancer-free women.
Extracellular vesicles-derived lncRNA SNHG12 maintains normal pregnancy by regulating p-STAT3/G-CSF axis to mediate the crosstalk between trophoblast and macrophage.
Biochem Pharmacol
July 2025
Reproductive Medical Center, Renmin Hospital of Wuhan University and Hubei Clinic Research Center for Assisted Reproductive Technology and Embryonic Development, Wuhan, China. Electronic address:
The crosstalk between trophoblasts and macrophages at the maternal-fetal interface is critical for the establishment and maintenance of normal pregnancy. Extracellular vesicles (EVs) have emerged as key mediators of intercellular communication, facilitating the exchange of biological signals between trophoblasts and macrophages. In this study, trophoblasts-derived EVs (Tro-Evs) were isolated by ultracentrifugation and characterized using nanoparticle tracking analysis, transmission electron microscopy and western blot.
View Article and Find Full Text PDF