[Bioinformatics analysis of efferocytosis-related genes in diabetic kidney disease and screening of targeted traditional Chinese medicine].

This study employed bioinformatics to screen the feature genes related to efferocytosis in diabetic kidney disease(DKD) and explores traditional Chinese medicine(TCM) regulating these feature genes. The GSE96804 and GSE30528 datasets were integrated as the training set, and the intersection of differentially expressed genes and efferocytosis-related genes(ERGs) was identified as DKD-ERGs. Subsequently, correlation analysis, protein-protein interaction(PPI) network construction, enrichment analysis, and immune infiltration analysis were performed.

Laboratory Investigation of Lipid Metabolic Reprogramming in Pulpitis: The Linolenic Acid-FASN-NR4A1 Axis in Modulating Dental Pulp Inflammation.

Aim: This study explores how lipid metabolic reprogramming contributes to the pathogenesis of pulpitis and identifies key molecular targets involved in regulating inflammation, with the goal of developing metabolic interventions to preserve dental pulp vitality.

Methodology: Primary human dental pulp cells (HDPCs) were stimulated with lipopolysaccharide (LPS) and subjected to integrated transcriptomic and metabolomic profiling to map inflammation-associated metabolic shifts. Functional validation included linolenic acid (LA) supplementation (alone or combined with a Fatty Acid Synthase (FASN) inhibitor, TVB-2640), Nuclear receptor subfamily 4 group A member 1 (NR4A1) knockdown, cytokine/NF-κB assessment (ELISA, Western blot), and lipid droplet/FASN visualisation (immunofluorescence).

Impact of visceral adiposity index on cognitive impairment and cognitive trajectories in Chinese middle-aged and older adults.

Introduction: The association between the visceral adiposity index (VAI) and cognitive function in middle-aged and older Chinese adults is not well understood. The "obesity paradox"-where obesity appears to be linked with better health outcomes-has also been observed. This study aims to clarify the association by using data from a nationally representative longitudinal survey.

Effects of sarcopenic obesity on depressive symptoms and their trajectories: findings from the CHARLS 2011-2020.

Purpose: Sarcopenia and obesity are recognized factors associated with the onset of depression. Our study aims to clarify the specific impact of sarcopenic obesity on depression development and the longitudinal trajectories of depressive symptoms.

Methods: Data from the 2011-2020 China Health and Retirement Longitudinal Study (CHARLS) were utilized, including 8532 participants aged ≥ 45 years.

Efficacy of Tripterygium glycosides in immune-mediated kidney diseases as a immunomodulation drug in combination with conventional immunosuppressive agents: a systematic review and meta-analysis of randomized controlled trials.

Background: Immune-mediated kidney diseases involve the immune system attacking the kidneys, resulting in damage and dysfunction. Tripterygium glycosides (TG) are known for their strong immunosuppressive and anti-inflammatory effects and are commonly used alongside traditional immunosuppressive agents. However, evidence-based support for the combined use of these treatments in immune-mediated kidney diseases remains insufficient and requires further validation.

Mechanisms of Xuefu Zhuyu decoction in treating diabetic kidney disease-induced renal fibrosis: UPLC-Q/TOF-MS, network pharmacology, and experimental validation.

Xuefu Zhuyu decoction (XFZY) has therapeutic effects on diabetic kidney disease (DKD)-induced renal interstitial fibrosis (RIF), but the mechanisms are unclear. This study investigates XFZY's molecular mechanisms through network pharmacology and experimental validation. Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and database screening was used to identify XFZY bioactive compounds.

Automatic Point Cloud Patching of Intraoral Three-Dimensional Scanning Based on Deep Learning.

Introduction And Aims: Intraoral scanning (IOS) captures real-time surface morphology of teeth and is widely used in clinical dentistry. However, due to the complex intraoral environment, data loss during scanning is common, leading to incomplete three-dimensional (3D) point clouds. This study aimed to develop and evaluate a deep learning-based method to automatically restore missing regions in intraoral 3D point clouds, thereby improving the accuracy and efficiency of digital orthodontic workflows.

Extracellular vesicles-derived lncRNA SNHG12 maintains normal pregnancy by regulating p-STAT3/G-CSF axis to mediate the crosstalk between trophoblast and macrophage.

The crosstalk between trophoblasts and macrophages at the maternal-fetal interface is critical for the establishment and maintenance of normal pregnancy. Extracellular vesicles (EVs) have emerged as key mediators of intercellular communication, facilitating the exchange of biological signals between trophoblasts and macrophages. In this study, trophoblasts-derived EVs (Tro-Evs) were isolated by ultracentrifugation and characterized using nanoparticle tracking analysis, transmission electron microscopy and western blot.

Uncovering the mechanism of resveratrol in the treatment of asthma: a network pharmacology approach with molecular docking and experimental validation.

Background: Evidence for the benefits of resveratrol (Res) in the treatment of asthma is progressively accumulating. However, the full spectrum of its molecular targets and the precise mechanisms remain incompletely characterized.

Method: Targets of Res were obtained from Swiss Target Prediction, TCMCP, and DrugBank.

Specialized pro-resolving mediators in neutrophil apoptosis regulation: unlocking novel therapeutic potential in kidney diseases.

Kidney diseases represent a diverse group of disorders with pathogenic mechanisms involving multiple pathological processes, including inflammation, immunity, and cell death. Neutrophils, as primary effector cells in inflammatory immune responses, participate in defending against renal infection and injury by releasing reactive oxygen species, proteases, and cytokines. However, persistent neutrophil activation is considered a crucial driver of kidney disease progression.

Dual-step photo-induced self-assembled hydrogel for endogenous oral mucosal wound healing.

By introducing piezoelectric materials into hydrogel oral dressings, a microelectric field could be generated under stress stimulation, thus facilitating oral wound healing. However, to adapt to the moist and dynamic environment of the oral cavity, traditional "step-by-step" synthesis often requires the combination of materials with different functionalities. Given the property differences between these materials, this strategy typically involves complex experimental procedures and unnecessary energy consumption.

Quantitative analysis and clinical determinants of orthodontically induced root resorption using automated tooth segmentation from CBCT imaging.

Background: Orthodontically induced root resorption (OIRR) is difficult to assess accurately using traditional 2D imaging due to distortion and low sensitivity. While CBCT offers more precise 3D evaluation, manual segmentation remains labor-intensive and prone to variability. Recent advances in deep learning enable automatic, accurate tooth segmentation from CBCT images.

Oleanolic acid derivative OA17 inhibits trophoblast apoptosis by suppressing HIF-1α nuclear translocation in SLE-associated adverse pregnancy outcomes.

Background: Systemic lupus erythematosus (SLE) primarily affects women of reproductive age and is associated with a high incidence of adverse pregnancy outcomes (APOs). Recent studies reveal that elevated HIF-1α expression in the placenta is involved in SLE-associated APOs and multiple pregnancy complications, but no clinically approved HIF-1α inhibitors exist to mitigate APOs. OA17, a pentacyclic triterpenoid derived from structural modification of oleanolic acid (OA), exhibits potent anti-inflammatory and antioxidant properties.

Association between serum α-klotho levels and the incidence of diabetic kidney disease and mortality in type 2 diabetes: evidence from a Chinese cohort and the NHANES database.

Background: The α-klotho is crucial in diabetes and its related complications. This study seeks to explore the link between α-klotho levels and the risk of diabetic kidney disease (DKD) as well as all-cause and cardiovascular mortality among individuals with type 2 diabetes mellitus (T2DM).

Methods: The investigation involved 126 Chinese T2DM patients and 4,451 individuals from the National Health and Nutrition Examination Survey (NHANES) database.

Diagnostic value of serum TGF-β1 and CysC in type 2 diabetic kidney disease: a cross-sectional study.

Background: Diabetic kidney disease (DKD) is one of the common microvascular complications of diabetes. The exploration of serum biomarkers holds promise for improving the efficiency and accuracy of early DKD diagnosis. This study aims to investigate the diagnostic value of transforming growth factor-β1 (TGF-β1) and cystatin C (CysC) in DKD patients.

HIF-1α mediates mitochondrial damage by down-regulating ALKBH7 expression to promote the aberrant activation of FLS in rheumatoid arthritis.

Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation and progressive joint destruction. Existing evidence indicates that hypoxia potentially contributes to the pathology of RA, though the specific mechanism remains unidentified. In this study, we explored the molecular mechanism through which the hypoxia-inducible factor (HIF-1α) contributed to the pathological process of RA.

FAD binding and dissociation in GMC-oxidoreductases.

The glucose-methanol-choline (GMC)-oxidoreductase superfamily comprises a large group of flavoenzymes such as glucose oxidase, glucose dehydrogenase and cellobiose dehydrogenase, which have been extensively studied and applied in biocatalysis and biosensors. Since the applicability of recombinant flavoenzymes is compromised by divergent glycosylation patterns and substoichiometric FAD occupancy, this study employed experimental and computational methods to analyze the deflavination and reconstitution of three GMC-oxidoreductases from a structural perspective. The results demonstrated that the amount of glycosylation of flavoenzymes is critical for both processes.

Astragaloside IV- loaded biomimetic nanoparticles target IκBα to regulate neutrophil extracellular trap formation for sepsis therapy.

This study explored the novel mechanism of Astragaloside IV (As) in treating sepsis and its application through a biomimetic nano-delivery system (As@ZM). Sepsis, a condition of organ dysfunction caused by an abnormal host response to infection, poses a significant threat to global health due to its high mortality rate. Our findings revealed a new mechanism for As in treating sepsis, which involved the reduction of neutrophil extracellular traps (NETs) release, potentially related to As binding with IκBα to inhibit the activation of the NF-κB pathway.

Automatic multimodal registration of cone-beam computed tomography and intraoral scans: a systematic review and meta-analysis.

Objectives: To evaluate recent advances in the automatic multimodal registration of cone-beam computed tomography (CBCT) and intraoral scans (IOS) and their clinical significance in dentistry.

Methods: A comprehensive literature search was conducted in October 2024 across the PubMed, Web of Science, and IEEE Xplore databases, including studies that were published in the past decade. The inclusion criteria were as follows: English-language studies, randomized and nonrandomized controlled trials, cohort studies, case-control studies, cross-sectional studies, and retrospective studies.

Enhancing Diabetic Oral Wound Healing with miR-132 Delivered Through Tetrahedral DNA Nanostructures.

Oral mucosal injuries are commonly caused by factors such as trauma, infection, or inflammation, especially in diabetic patients where healing is difficult and significantly affects quality of life. In this study, a nanocarrier system based on DNA tetrahedrons (TDN) is developed, which serve as ideal vectors due to their excellent intracellular uptake and drug delivery capabilities. By efficiently delivering miR132 into cells, the proliferation and migration of human oral mucosal fibroblasts (HOMFs) and human umbilical vein endothelial cells (HUVECs) are regulated, along with the modulation of inflammation and antioxidant processes.

Pharmacological effects and mechanisms of danlong oral liquid in asthma airway remodeling: Insights from serum medicinal chemistry, network pharmacology, and experimental validation.

Ethnopharmacological Relevance: Danlong oral liquid (DLOL) is a traditional Chinese proprietary medicine commonly used to treat chronic respiratory diseases, including bronchial asthma and chronic obstructive pulmonary disease. However, the therapeutic effects and pharmacological mechanisms of DLOL in improving airway remodeling remain unclear.

Aims Of The Study: This study utilizes in vivo and in vitro experiments, serum pharmacological analysis, and network-based pharmacology approaches to investigate the effects and mechanisms of DLOL on airway remodeling and epithelial-mesenchymal transition (EMT) in asthma.

Predictive value of bone metabolism markers in the progression of diabetic kidney disease: a cross-sectional study.

Objective: This study aimed to investigate the relationship between bone metabolism markers, including serum klotho, fibroblast growth factor 23 (FGF23), 25(OH)D3, iPTH, calcium (Ca), and PHOS and the progression of diabetic kidney disease (DKD) in patients with type 2 diabetes mellitus (T2DM). Additionally, the predictive value of these markers for DKD progression was evaluated.

Methods: This study involved 126 patients with T2DM between May 2021 and March 2023.

KLF4 regulates trophoblast function and associates with unexplained recurrent spontaneous abortion.

Background: Recurrent spontaneous abortion (RSA) is defined as two or more consecutive spontaneous abortions before 20 weeks with the same spouse [1]. However, approximately 50% of RSA cases of unknown cause are classified as unexplained recurrent spontaneous abortion (URSA). Potential factors include decreased trophoblast cell migration and invasion, leading to impaired placental implantation and maintenance of the normal maternal-fetal interface.