Article Synopsis
- Mammalian cells contain around 30,000 times more protein molecules than mRNA, highlighting a key challenge in proteomics technology development.
- Strategies for counting billions of protein molecules using liquid chromatography-tandem mass spectrometry (LC-MS/MS) are discussed.
- These strategies may improve single-molecule methods by addressing the difficulties posed by the extensive dynamic range of the proteome.
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Article Abstract
Mammalian cells have about 30,000-fold more protein molecules than mRNA molecules, which has major implications in the development of proteomics technologies. We review strategies that have been helpful for counting billions of protein molecules by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and suggest that these strategies can benefit single-molecule methods, especially in mitigating the challenges of the wide dynamic range of the proteome.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10044470 | PMC |
| http://dx.doi.org/10.1038/s41592-023-01802-5 | DOI Listing |
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