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Partial denitrification is an alternative process to provide stable nitrite for anammox. In this study, based on full-scale and lab-scale experiments, achieving and control of partial denitrification and the microbial mechanism were studied for 17 months in municipal wastewater treatment plant (MWWTP). Using glucose (GLC) as sole carbon source, partial denitrification was successfully achieved with nitrite accumulation percentage (NAP) higher than 90%; whereas, using sodium acetate (NaAc) as sole carbon source, nitrite accumulation was effectively controlled with economic and efficient carbon usage. Candidatus Competibacter and Thaurea were the dominant communities for partial denitrification. Denitrifying glycogen accumulating organisms (DGAOs), Thauera, denitrifying phosphorus accumulating organisms (DPAOs), GAOs, PAOs and denitrifiers coexisted in MWWTP, resulting in COD specific removal rate (CODSRR) of 883.10 ~ 1188.92mgN/gMLVSS/h during partial denitrification. Through adjustment of Anoxic-Oxic (A/O) operation to anoxic operation, the growth of GAOs and PAOs could be limited.
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http://dx.doi.org/10.1016/j.biortech.2021.125765 | DOI Listing |
Environ Res
September 2025
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, PR China.
Partial denitrification coupled with anammox (PD/A) has emerged as a promising low-carbon strategy for energy-efficient nitrogen removal from municipal wastewater. However, the reactivation of PD/A systems following operational disturbances remains challenging, particularly under continuous-flow conditions, where microbial interactions and process stability are more complex than in sequencing batch reactors. This study systematically and first evaluated the recovery dynamics of a continuous-flow PD/A process seeded with low-activity granular sludge stored at 4 °C for three months.
View Article and Find Full Text PDFJ Environ Manage
September 2025
Department of Civil and Environmental Engineering, Western University, 1151 Richmond Street, London, ON, N6A 3K7, Canada. Electronic address:
J Hazard Mater
August 2025
School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, China; Hua An Biotech Co., Ltd., Foshan 528300, China. Electronic address:
Rare earth mining activities cause severe nitrogen pollution in watersheds, yet the residual hazardous rare earth elements (REEs) toxicity in tailings wastewater challenges biological nitrogen removal technology. This work demonstrated that introducing low-dose Fe(II) into partial denitrification/anammox (PD/A) system significantly alleviated REEs-induced stress on anammox consortia via detoxification and physical barrier reinforcement. The PD/A bioreactor with 15 mg/L Fe(II) (R1) was compared against a control without Fe(II) for real rare earth tailings wastewater treatment.
View Article and Find Full Text PDFMicroorganisms
August 2025
The Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, China.
The Sanniang Bay (SNB) and Dafeng River Estuary (DFR) in the Northern Beibu Gulf, China, are critical habitats for the Indo-Pacific humpback dolphin (). However, whether and how the decreased dissolved oxygen (DO) has happened in bottom seawater remains poorly understood. This study investigated DO depletion and microbial community responses using a multidisciplinary approach.
View Article and Find Full Text PDFEnviron Microbiome
August 2025
Department of Biology, Georgia Southern University, Statesboro, GA, 30460-8042, USA.
Protozoa-driven micro-food webs are pivotal regulators of microbial community structure and carbon-nitrogen cycling. By mediating trophic cascades that regulate bacterial and algal populations, protozoa influence nutrient remineralization and energy flow. Their regulation is crucial for stabilizing biogeochemical processes and preventing harmful algal blooms.
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