Fingerprinting profile of polysaccharides from Lycium barbarum using multiplex approaches and chemometrics.

Int J Biol Macromol

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address:

Published: July 2015


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Techniques including ultraviolet-visible spectra (UV), high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were used in the fingerprinting analysis of Lycium barbarum polysaccharides (LBPs) from different locations and varieties. Multiple fingerprinting profiles were used to evaluate the similarity and classification of different LBPs with the help of chemometrics. The results indicated that sixteen batches of LBPs had good consistency, and fingerprinting techniques were simple and robust for quality control of LBPs as well as related products. In addition, fingerprinting techniques combined with chemometrics could also be used to identify different cultivation locations of LBPs samples. Finally, four monosaccharides (galacturonic acid, glucose, galactose and arabinose) and the absorptions of stretching vibration of ester carbonyl groups as well as NH variable angle vibration of -CONH- could be selected as herbal markers to distinguish different samples.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2015.03.062DOI Listing

Publication Analysis

Top Keywords

lycium barbarum
8
fingerprinting techniques
8
fingerprinting
5
lbps
5
fingerprinting profile
4
profile polysaccharides
4
polysaccharides lycium
4
barbarum multiplex
4
multiplex approaches
4
approaches chemometrics
4

Similar Publications

Lycium barbarum alleviates oxidative stress-induced ferroptosis and enhances mitophagy in intervertebral disc degeneration.

Cell Signal

September 2025

Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China; Molecular Pharmacology Research Center, School of Pharmaceutical Sciences; Zhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, China. Electronic address:

Lycium barbarum is a traditional Chinese medicine that has been demonstrated to exhibit a wide variety of biological functions, such as antioxidation, neuroprotection, and immune modulation. The therapeutic effect of Lycium barbarum on intervertebral disc degeneration (IVDD) has not been conclusively established. In our study, we investigated the mechanisms of Lycium barbarum extract (LBE) using Network pharmacology and bioinformatic analyses.

View Article and Find Full Text PDF

Lycium barbarum polysaccharides alleviate experimental autoimmune prostatitis by putrescine/TRAF/JAK/STAT-mediated Th17 differentiation.

Phytomedicine

August 2025

Department of Urology, the First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, Anhui, PR China; Institute of Urology, Anhui Medical University, Hefei, Anhui, PR China; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui,

Background: Due to its often refractory nature, chronic prostatitis (CP) is a prevalent disorder that notably impacts the quality of life of 20-40-year-old men worldwide. Lycium barbarum polysaccharides (LBPs) have a broad spectrum of biological activities, such as immune modulation and the ability to reduce blood glucose and lipid levels. However, whether they modulate the gut microbiota and metabolites to exert their anti-inflammatory effects remains to be explored.

View Article and Find Full Text PDF

Design of lightweight neural network model for provenance discrimination of Lycium barbarum based on hyperspectral imaging technology.

Spectrochim Acta A Mol Biomol Spectrosc

August 2025

College of Mechanical and Electronic Engineering, Nanjing Forestry University, Nanjing 210037, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China. Electronic address:

The study aimed to build a lightweight neural network model for Lycium barbarum provenance discrimination using hyperspectral imaging technology. Firstly, hyperspectral images (336.2-1038.

View Article and Find Full Text PDF

Response of sp. to Nitrogen Input and Its Potential Role in Rhizosphere Enrichment of .

Microorganisms

August 2025

National Wolfberry Engineering Research Center, Wolfberry Science Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China.

L. (goji berry), a traditional Chinese medicinal plant, depends heavily on nitrogen input to maintain productivity. Nitrogen application also profoundly influences rhizosphere microbial dynamics, which are critical for soil health and plant performance.

View Article and Find Full Text PDF