GCLM lactylation mediated by ACAT2 promotes ferroptosis resistance in KRAS-mutant cancer.

KRAS mutations drive tumorigenesis, but their role in ferroptosis regulation remains unclear. Here, we construct wild-type KRAS (KRAS) and KRAS-mutant cancer cells and demonstrate that G12D-mutant cells exhibit increased viability and reduced ferroptosis upon RSL3 or erastin treatment. These cells show diminished lipid peroxidation and mitochondrial damage, indicating ferroptosis resistance.

Simply structural, reversible and chemical stable ratiometric pH-responsive fluorescence probe of pyrene derivatives and its applications.

The pyrene group is commonly used as a fluorophore in ratiometric fluorescence probes. However, these probes almost exclusively utilize the monomer/excimer of the pyrene group to achieve ratiometric fluorescence recognition for analytes. In the study, three structurally simple, mono-substituted pyrene derivatives, namely pyrene-1-ol (Probe1), pyren-1-ylboronic acid (Probe2), pyrene-1-carboxylic acid (Probe3) utilize their protonation or deprotonation effects to influence the electronic excited state of the pyrene group, thereby achieving sensitive ratiometric fluorescence recognition of pH.

Pan-cancer analysis of the prognosis and immune infiltration of NSUN7 and its potential function in renal clear cell carcinoma.

Background: NSUN7, an enzyme responsible for the RNA m5c modification, has been recognized as a valuable indicator for predicting and diagnosing an array of cancer. Nevertheless, there is still a scarcity of thorough analyses exploring its diagnostic, predictive, and immune system-related importance in various types of cancer.

Methods: We integrated multiple publicly available databases, including TCGA, TISIDB, TISCH2, and UALCAN, to comprehensively investigate the role of NSUN7 in pan-cancer across various omics data types.

DTX2 attenuates Lenvatinib-induced ferroptosis by suppressing docosahexaenoic acid biosynthesis through HSD17B4-dependent peroxisomal β-oxidation in hepatocellular carcinoma.

Aims: Emerging resistance to Lenvatinib, which is used as a first-line agent for the treatment of advanced hepatocellular carcinoma (HCC), is still a concern. The aim of this study was to determine core factors of Lenvatinib resistance (LR) and their underlying molecular mechanisms.

Methods: CRISPR screening in HCC cells was conducted, which identified E3 ubiquitin ligase deltex 2 (DTX2) as a core LR-related gene.

Highly crystalline carbon nitride induced by ultralow dosage of sodium fluoride with pinning-docking mechanism.

In the typical ionothermal synthesis of crystalline carbon nitride (CCN), alkali metal halides are usually used in large amounts. Here, we report a new method for synthesizing poly (heptazine imide) (PHI) using only a trace amount of NaF, which is 20 times less than the amount of NaCl typically required to achieve the PHI structure. Different from the prevailing view that salts function primarily as templates and chelating agents during polymerization, our research revealed the unique role that NaF plays in the polymerization of PHI.

Evaporation-Assisted Synthesis of Olympic Gels.

Catenated networks exclusively composed of intertwining rings were first envisioned as "Olympic gels" by Pierre-Gilles de Gennes four decades ago but have not been successfully prepared in artificial materials yet due to the challenge in synthesis. Herein, we present a bio-inspired, evaporation-assisted strategy to address this issue. In our design, the evaporation of liquid catalysts that induce ring-chain equilibrium of polymer systems drives macrocycles to encounter and assists their catenation through reversible cyclization.

Why are people with dementia overlooked in long-term care insurance policy in Guangzhou, China?

Background And Objectives: There is a growing demand for dementia care to be funded by long-term care insurance (LTCI). However, evidence indicates that people with dementia are overlooked in China's LTCI policy and empirical research on this issue is notably scarce. Among the first seven LTCI pilot cities that officially enrolled people with dementia, Guangzhou is unique for roll-back LTCI policies related to eligibility criteria and benefits.

Epistasis between genetic variations on MdMYB109 and MdHXK1 exerts a large effect on sugar content in apple fruit.

Many quantitative traits are controlled by multiple genetic variations with minor effects, making it challenging to resolve the underlying genetic network and to apply functional markers in breeding. Affected by up to a hundred quantitative trait loci (QTLs), fruit-soluble sugar content is one of the most complex quantitative traits in apple (Malus sp.).

Advances in Engineering Circular RNA Vaccines.

Engineered circular RNAs (circRNAs) are a class of single-stranded RNAs with head-to-tail covalently linked structures that integrate open reading frames (ORFs) and internal ribosome entry sites (IRESs) with the function of coding and expressing proteins. Compared to mRNA vaccines, circRNA vaccines offer a more improved method that is safe, stable, and simple to manufacture. With the rapid revelation of the biological functions of circRNA and the success of Severe Acute Respiratory Coronavirus Type II (SARS-CoV-2) mRNA vaccines, biopharmaceutical companies and researchers around the globe are attempting to develop more stable circRNA vaccines for illness prevention and treatment.

Prognostic significance of glucose-lipid metabolic index in pancreatic cancer patients with diabetes mellitus.

Background: The incidence of pancreatic cancer (PC) is higher in diabetic patients due to disturbances in glucose and lipid metabolism caused by insulin resistance (IR). However, the effect of diabetes as well as IR on the prognosis of PC patients remains inconclusive. Our study aims to assess the impact of IR on the prognosis of PC patients with diabetes.

Olympic gels formed through catenation of dsDNA rings regulated by topoisomerase II: A coarse-grained model.

Topological regulation of DNA by topoisomerases in cells is very crucial for life. We propose a coarse-grained model to study the catenation process of double-stranded DNA (dsDNA) rings regulated by topoisomerase II (TOP2) and provide a computational method to characterize the topological structures of the Olympic gels obtained. The function of TOP2 in the catenation of dsDNA rings is implicitly fulfilled by operating the length of a stretchable catch bond in the dsDNA ring.

Identification and validation of stemness-based and ferroptosis-related molecular clusters in pancreatic ductal adenocarcinoma.

Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy with an extremely poor prognosis. Cancer stem cells (CSCs) are considered to be responsible for the poor survival, recurrence and therapy resistance of PDAC. Ferroptosis plays a crucial role in the sustain and survival of CSCs.

Genome-wide CRISPR/Cas9 screening for drug resistance in tumors.

Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) screening is a simple screening method for locating loci under specific conditions, and it has been utilized in tumor drug resistance research for finding potential drug resistance-associated genes. This screening strategy has significant implications for further treatment of malignancies with acquired drug resistance. In recent years, studies involving genome-wide CRISPR/Cas9 screening have gradually increased.

Surgical treatment of primary hepatic neuroendocrine tumor diagnosed by AlF-NOTA-Octreotide PET/CT: a case report.

Neuroendocrine tumors (NETs) are a heterogeneous group of tumors originating from peptide-producing neurons and neuroendocrine cells. The liver is the most common site of metastasis for NETs, while primary hepatic neuroendocrine tumors (PHNETs) are exceedingly rare. While somatostatin receptor scintigraphy (SRS) has demonstrated superior efficacy compared to [F]FDG PET imaging in the diagnosis of neuroendocrine tumors, [F]AlF-NOTA-Octreotide ([F]AlF-OC) PET/CT also exhibits specific advantages over SRS.

Usage of a simplified blumgart pancreaticojejunostomy in laparoscopic pancreaticoduodenectomy: a single center experience.

Background: Blumgart pancreaticojejunostomy (PJ) was shown to be an effective method for pancreaticojejunostomy in open pancreaticoduodenectomy. But the original Blumgart method is involved in complicated and interrupted sutures, which may not be suitable for the laparoscopic approach. In this study, we introduced a simplified Blumgart method for laparoscopic pancreaticojejunostomy.

Regulation of iron metabolism and ferroptosis in cancer stem cells.

The ability of cancer stem cells (CSCs) to self-renew, differentiate, and generate new tumors is a significant contributor to drug resistance, relapse, and metastasis. Therefore, the targeting of CSCs for treatment is particularly important. Recent studies have demonstrated that CSCs are more susceptible to ferroptosis than non-CSCs, indicating that this could be an effective strategy for treating tumors.

Case Report: Camrelizumab combined with gemcitabine and oxaliplatin in the treatment of advanced intrahepatic cholangiocarcinoma: a case report and literature review.

Intrahepatic cholangiocarcinoma (ICC) is one of the most common invasive malignant tumors, with a 5-year survival rate of less than 5%. Currently, radical surgical resection is the preferred treatment for ICC. However, most patients are only diagnosed at an advanced stage and are therefore not eligible for surgery.

Polymer-Based Long-Lived Phosphorescence Materials over a Broad Temperature Based on Coumarin Derivatives as Information Anti-Counterfeiting.

The development of new polymer-based room-temperature phosphorescence materials is of great significance. By a special molecule design and a set of feasible property-enhancing strategies, coumarin derivatives (CMDs, M-M) were doped into polyvinyl alcohol (PVA), polyacrylamide (PAM), corn starch, and polyacrylonitrile (PAN) as information anti-counterfeiting. CMDs-doped PVA and CMDs-doped corn starch films showed long-lived phosphorescence emissions up to 1246 ms (M-PVA) and 697 ms (M-corn starch), reaching over 10 s afterglow under naked eye observation under ambient conditions.

A case report of surgical resection treatment for complete remission after chemotherapy for advanced pancreatic cancer.

Pancreatic cancer is a common type of cancer that is treated using surgery or chemotherapy. However, for patients who cannot have surgery, the treatment options are limited and have a low success rate. We report a case of a patient with locally advanced pancreatic cancer who was unable to have surgery due to a tumor that had invaded the coeliac axis and portal vein.

Preparation and Properties of Poly(butylene adipate-co-terephthalate)/thermoplastic Hydroxypropyl Starch Composite Films Reinforced with Nano-Silica.

The use of biodegradable plastics is gradually increasing, but its expensive cost limits promotion. In this study, poly(butylene adipate-co-terephthalate)/thermoplastic hydroxypropyl starch reinforced with nano-silica (PBAT/TPHS) composite films with high hydroxypropyl starch content were prepared in a two-step process. The effect of reinforced thermoplastic hydroxypropyl starch on the mechanical, thermal, processing properties, and micromorphology of the composite films was investigated.

High Expression of COL10A1 Is an Independent Predictive Poor Prognostic Biomarker and Associated with Immune Infiltration in Advanced Gastric Cancer Microenvironment.

Medical technology has become more and more sophisticated recently, which, however, fails to contribute to a better prognosis for patients suffering advanced gastric cancer (GC). Hence, new biomarkers specific to GC diagnosis and prognosis shall be identified urgently. This study screened differentially expressed genes (DEGs) between 375 GC samples and 32 paracancer tissue samples from TCGA datasets.

In Vitro Response of Immune Cells on Metal Oxide Nanoparticles with Different Solubility.

In this study, three engineered metal oxide nanoparticles (NPs) with similar size but significantly different solubility were used to study the cellular uptake, toxicity and immune activation of Raw 264.7 cells. Commercially available TiO2, Fe2O3 and ZnO NPs were used in this study.