INTS7 modulates cell proliferation and apoptosis via promoting cell cycle progression in lung adenocarcinoma.

The evolutionarily conserved Integrator complex, which is composed of over 10 subunits, orchestrates diverse RNA-processing events such as 3'-end maturation of small nuclear RNAs (snRNAs), transcription termination of RNA Polymerase II, and DNA damage response signaling pathways; however, the functional roles of individual Integrator complex subunits in lung adenocarcinoma (LUAD) remain poorly characterized, and this study aimed to systematically investigate the potential oncogenic functions and prognostic values of these subunits in LUAD. To achieve this goal, the expression profiles of Integrator complex subunits were profiled using transcriptomic data from the The Cancer Genome Atlas (TCGA) database, survival analyses (including Kaplan-Meier and Cox regression models) were performed to evaluate the correlations between subunit expression levels and patient survival outcomes (overall survival (OS) and disease-free survival (DFS)), co-expression network analysis was conducted to annotate the potential biological functions of key subunits, and functional validation was performed using CCK-8 assays and flow cytometry to assess the impact of INTS7 depletion on cell proliferation and cycle progression in LUAD cell lines. The findings of this study showed that Integrator complex subunits were significantly overexpressed in LUAD tissues compared to normal lung parenchyma; among these subunits, INTS7 expression was most strongly associated with shortened OS and DFS, indicating its pivotal role in LUAD pathogenesis, while bioinformatics analyses revealed that INTS7 is involved in regulating critical biological processes including cell cycle progression, transcriptional regulation, and RNA metabolism, and loss-of-function experiments demonstrated that genetic silencing of INTS7 significantly inhibited cell proliferation and induced cell cycle arrest in LUAD cells.

B4GALT3 as a Key Glycosyltransferase Gene in Multiple Myeloma Progression: Insights From Bioinformatics, Machine Learning, and Experimental Validation.

Glycosylation abnormalities are critical in the progression of various cancers. However, their role in the onset and prognosis of multiple myeloma (MM) remains underexplored. This study aims to identify glycosyltransferase (GT)-related biomarkers and investigate their underlying mechanisms in MM.

Regulation of type I interferon factor secretion via the TLR3 signaling pathway after PRV infection of mouse trigeminal ganglion cells.

This study investigates the effects of pseudorabies virus (PRV) infection on the antiviral immune signaling pathway and type I interferon factors in mouse trigeminal ganglion (TG) cells. The experiment involved inoculating TG primary cells with PRV and intranasally infecting mice. The results indicated that PRV infection of mouse TG primary cells led to alterations in the gene and protein expression of TLR3, TRIF, TBK1, and IRF3, while inhibiting the expression of IκBα protein in the later stages of infection.

The impact of ultrasound contrast-enhanced fusion virtual navigation technology-guided microwave ablation (MWA) and standard MWA on the local tumor progression of hepatocellular carcinoma with unclear ultrasound images.

Purpose: Compared the impact of ultrasound contrast-enhanced fusion virtual navigation technology-guided microwave ablation (MWA) and standard MWA on the local tumor progression of hepatocellular carcinoma with unclear ultrasound images, to explore the utility of virtual navigation ultrasound contrast-enhanced imaging.

Methods: A retrospective analysis was conducted on clinical data from hepatocellular carcinoma patients admitted between June 2021 and August 2022, all of whom had liver lesions suggested by Computed Tomography (CT) or Magnetic Resonance (MR) but not visualized on 2D ultrasound. They underwent ultrasound contrast-enhanced fusion virtual navigation technology-guided microwave ablation and standard microwave ablation, and comparisons were made between the two groups in terms of general information, blood parameters, ultrasonic test, intraoperative indicators, ablation indicators, postoperative complications, recurrence indicators, local progression and long-term outcomes.

Advantage of Tolerability following Arsenic Trioxide-VTD vs VRD in newly diagnosed multiple myeloma patients: a prospective, open-label study.

Multiple myeloma is the second most common hematologic malignancy in older patients. The standard front-line VRD regimen (bortezomib/lenalidomide/dexamethasone) achieves high efficacy but is associated with significant toxicity, leading to infections, bone marrow suppression, and treatment discontinuation in approximately 20% of patients. Alternative regimens with reduced toxicity are needed for this demographic.

Shape memory composite membrane with widely programmable electromagnetic shielding effectiveness.

The application of shape memory smart materials in the field of electromagnetic interference (EMI) shielding can compensate for their poor adaptability and promote the development of electromagnetic shielding composite materials towards multi-functionality and intelligence. Herein, an intelligent poly(ethylene--vinyl acetate)/polydopamine/MXene (EVA/PDA/MXene) composite membrane with tunable electromagnetic shielding capability over a wide range was prepared using electrospinning and dip-coating techniques. Firstly, electrospinning technology was used to prepare highly cross-linked EVA fiber membranes.

LightRoseTTA: High-Efficient and Accurate Protein Structure Prediction Using a Light-Weight Deep Graph Model.

Accurately predicting protein structure, from sequences to 3D structures, is of great significance in biological research. To tackle this issue, a representative deep big model, RoseTTAFold, is proposed with promising success. Here, "a light-weight deep graph network, named LightRoseTTA," is reported to achieve accurate and highly efficient prediction for proteins.

Ultrasound-guided drug injection combined with mini-needle knife therapy for acute lumbar sprain: a prospective interventional study.

Purpose: To evaluate the clinical efficacy of ultrasound-guided local anesthetic injection combined with myofascial trigger point mini-needle knife therapy for acute lumbar sprain management.

Methods: In this prospective study, 60 patients (January-July 2024) received ultrasound-guided injections at the third lumbar (L3) transverse process attachment and posterior medial spinal nerve branch, combined with miniscalpel-needle release of MTrPs. Visual analog scale (VAS), modified Oswestry disability index (MODI), and lumbar range of motion (ROM) were assessed pre-treatment and on days 3 and 7 post-treatment.

Hydrothermal pretreatment for enhanced thermochemical or biochemical conversion of pharmaceutical biowastes into fuels, fertilizers, and carbon materials.

Pharmaceutical biowastes, rich in organic matter and high in moisture, are typical light industry byproducts with waste and renewable attributes. Thermochemical and biochemical conversion technologies transform these residues into value-added bioproducts, including biofuels, biofertilizers, and bio-carbon materials. Hydrothermal pretreatment effectively removes toxic substances and enhances feedstock for these processes.

Research progress on the molecular mechanism of immune escape of porcine reproductive and respiratory syndrome virus.

Porcine reproductive and respiratory syndrome (PRRS), caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is a severe and highly contagious disease that results in significant economic losses for the pig industry. Currently, vaccination is one of the most effective methods for controlling PRRS; however, due to the extensive genetic variation of PRRSV and the generation of homologous immunity, vaccines provide protective effects only against homologous strains and lack effective cross-protection against heterologous strains. Furthermore, PRRSV encodes a variety of proteins with immune escape functions, and the mechanisms underlying these functions are complex and not yet fully understood.

Tailoring Intrinsic Oxygen Vacancies in P3-Type NaMnMgO to Enhance Oxygen Redox Activity.

Integrating oxygen redox reactions with transition metal redox reactions offers a promising strategy to double or triple the energy density for large-capacity battery cathodes. In this study, we have introduced intrinsic oxygen vacancies (V) into a P3 layered cathode to modulate the electronic structure of O atoms and enhance oxygen redox activity. Rietveld-refined X-ray diffraction (XRD), X-ray absorption spectroscopy (XAS), and X-ray photoelectron spectroscopy confirm the successful creation of V in NaMnMgO (OV-NMM).

Sintilimab (anti-PD-1 antibody) combined with high-dose methotrexate, temozolomide, and rituximab (anti-CD20 antibody) in primary central nervous system lymphoma: a phase 2 study.

Primary central nervous system lymphoma (PCNSL) is a rare and frequently fatal lymphoma subtype. The programmed death-1 (PD-1) pathway has emerged as a potential therapeutic target, but the effectiveness of PD-1 antibody sintilimab in combination with immunochemotherapy as a frontline treatment for PCNSL remains to be determined. In this phase 2 trial (ChiCTR1900027433) with a safety run-in, we included patients aged 18-70 with newly diagnosed PCNSL.

Role of BACH1 in multiple myeloma.

Objective: Examine Bach1 protein expression in bone marrow biopsy specimens obtained from newly diagnosed multiple myeloma (NDMM) and iron deficiency anemia (IDA) patients. Conduct a thorough analysis to explore the potential connection between Bach1 and the onset as well as treatment response of NDMM.

Methods: This study investigated Bach1 expression in bone marrow biopsy tissues from NDMM and IDA patients.

Bioremoval of Co(II) by a novel halotolerant microalgae sp. FACHB-558 from saltwater.

Cobalt pollution is harmful to both the aquatic ecosystem and human health. As the primary producer of aquatic ecosystems in hypersaline environments, unicellular planktonic microalgae is considered to be a low-energy and eco-friendly biosorbent that removes excess cobalt and enhances the vitality of coastal and marine ecosystems. In this study, we found that the halotolerant microalga named sp.

NCX1/Ca promotes autophagy and decreases bortezomib activity in multiple myeloma through non-canonical NFκB signaling pathway.

Although bortezomib (BTZ) is the cornerstone of anti-multiple myeloma (MM) therapy, the inevitable primary and secondary drug resistance still seriously affects the prognosis of patients. New treatment strategies are in need. Sodium-calcium exchanger 1 (NCX1) is a calcium-permeable ion transporter on the membrane, and our previous studies showed that low NCX1 confers inferior viability in MM cells and suppressed osteoclast differentiation.

[Ga]Pentixafor PET/CT for staging and prognostic assessment of newly diagnosed multiple myeloma: comparison to [F]FDG PET/CT.

Purpose: To evaluate the prognostic performance of [Ga]Pentixafor PET/CT at baseline for staging of patients with newly diagnosed multiple myeloma (MM) and to compare it with [F]FDG PET/CT and the Revised-International Staging System (R-ISS).

Methods: Patients who underwent [Ga]Pentixafor and [F]FDG PET/CT imaging were retrospectively included. Patient staging was performed according to the Durie-Salmon PLUS staging system based on [Ga]Pentixafor PET/CT and [F]FDG PET/CT images, and the R-ISS.

SSBP1 is a novel prognostic marker and promotes disease progression via p38MAPK signaling pathway in multiple myeloma.

Multiple myeloma (MM) remains an incurable disease. Identification of meaningful co-expressed gene clusters or representative biomarkers of MM may help to identify new pathological mechanisms and promote the development of new therapies. Here, we performed weighted sgene co-expression network analysis and a series of bioinformatics analysis to identify single stranded DNA binding protein 1 (SSBP1) as novel hub gene associated with MM development and prognosis.

Combination of serum FOXR2 and transvaginal three-dimensional power Doppler ultrasonography in the diagnosis of uterine lesions.

Background: Cervical carcinoma and endometrial carcinoma are the most common gynecologic cancers worldwide. Forkhead-box R2 (FOXR2) plays an important role in the progression of various malignant tumors. However, the effects of FOXR2 on the development of uterine lesions remain unclear.

Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens.

African swine fever (ASF) is an infectious disease caused by African swine fever virus (ASFV) that is highly contagious and has an extremely high mortality rate (infected by virulent strains) among domestic and wild pigs, causing huge economic losses to the pig industry globally. In this study, SDS-PAGE gel bands hybridized with ASFV whole virus protein combined with ASFV-convalescent and ASFV-positive pig serum were identified by mass spectrometry. Six antigens were detected by positive serum reaction bands, and eight antigens were detected in ASFV-convalescent serum.

Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase.

β-Carotene is one of the economically important carotenoids, having functions as the antioxidant to remove harmful free radicals and as the precursor for vitamin A and other high-valued xanthophyll such as zeaxanthin and astaxanthin. Lycopene cyclase plays an important role in the branching of β-carotene and α-carotene. Aiming to develop the microalgae with enhanced β-carotene productivity, the CrtY gene from bacterium Pantoea agglomerans was integrated into Chlamydomonas reinhardtii.

Membrane fusion, potential threats, and natural antiviral drugs of pseudorabies virus.

Pseudorabies virus (PrV) can infect several animals and causes severe economic losses in the swine industry. Recently, human encephalitis or endophthalmitis caused by PrV infection has been frequently reported in China. Thus, PrV can infect animals and is becoming a potential threat to human health.

USC-ENet: a high-efficiency model for the diagnosis of liver tumors combining B-mode ultrasound and clinical data.

Purpose: Ultrasound image acquisition has the advantages of being low cost, rapid, and non-invasive, and it does not produce radiation. Currently, ultrasound is widely used in the diagnosis of liver tumors. However, owing to the complex presentation and diverse features of benign and malignant liver tumors, accurate diagnosis of liver tumors using ultrasound is difficult even for experienced radiologists.

Overexpression of 18S rRNA methyltransferase CrBUD23 enhances biomass and lutein content in .

Post-transcriptional modification of nucleic acids including transfer RNA (tRNA), ribosomal RNA (rRNA) and messenger RNA (mRNA) is vital for fine-tunning of mRNA translation. Methylation is one of the most widespread post-transcriptional modifications in both eukaryotes and prokaryotes. HsWBSCR22 and ScBUD23 encodes a 18S rRNA methyltransferase that positively regulates cell growth by mediating ribosome maturation in human and yeast, respectively.