Perirenal fat thickness may be a significant predictor of prognosis and postoperative renal function in renal cancer surgery patients.

Renal cell carcinoma accounts for a significant number of kidney malignancy-related fatalities globally. Perirenal Fat Thickness (PRFT) may indicate a state of nutritional excess in patients, which is potentially directly linked to both the incidence and prognosis of kidney cancer. This study investigated the association between perirenal fat thickness (PRFT) and overall survival (OS), as well as the predictive value of PRFT for postoperative estimated glomerular filtration rate (eGFR) in patients with renal cell carcinoma (RCC).

Parenchymal volume and functional recovery after clamped partial nephrectomy: potential discrepancies.

Background: Parenchymal-volume-analysis (PVA) appears superior to nuclear-renal-scans (NRS) for assessing split-renal-function (SRF). Our objective was to evaluate how ischemia during PN impacts the accuracy of PVA for estimating functional outcomes.

Methods: Partial nephrectomy (PN) patients (2010-2022) with pre/postoperative NRS and cross-sectional imaging were retrospectively analyzed.

Compact full-field modulation of optical vector fields using a single digital micromirror device.

The ability to generate complex optical vector fields with precise control over amplitude, phase, and polarization is essential for advanced applications in optical imaging, communication, and manipulation. In this work, we present a superpixel-based encoding method that enables full-field vector modulation using a single digital micromirror device (DMD). Each superpixel encodes two complex values corresponding to orthogonal polarization components, which are spatially separated and filtered through a 4 optical system to reconstruct the target field.

High-speed single-exposure time-reversed ultrasonically encoded optical focusing into scattering media with off-axis holography.

Optical scattering limits the ability to focus light deep inside scattering media, posing a long-standing challenge in biomedical applications such as deep-tissue imaging and photodynamic therapy. Digital optical phase conjugation (DOPC), combined with ultrasonic guide stars-an approach named as time-reversed ultrasonically encoded (TRUE) optical focusing-has shown promise in overcoming this limitation. However, practical applications, such as imaging in living tissues, require TRUE systems to operate at high speeds to compensate for dynamic scattering caused by physiological motion.

Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE).

Single-pixel imaging (SPI) is a promising technology for optical imaging beyond the visible spectrum, where commercial cameras are expensive or unavailable. However, limitations such as slow pattern projection rates and time-consuming reconstruction algorithms hinder its throughput for real-time imaging. Consequently, conventional SPI is inadequate for high-speed, high-resolution tasks.

[Clinical analysis of clavicle anatomical plate in the treatment of AllmanⅡC type of clavicle fracture].

Objective: To evaluate the clinical efficacy and safety of clavicular anatomical plate in the treatment of clavicular Allmanic fracture.

Methods: Between January 2016 and December 2022, 27 patients with Allman ⅡC distal clavicle fractures were treated with clavicular anatomical plate. There were 19 males and 8 females, aged from 16 to 69 years old, with an average of (39.

Adaptive central moment descent for broadband scattered light manipulation.

Wavefront shaping enables precise control of light propagation through scattering media by utilizing the knowledge of the scattering matrix. However, in many imaging applications where broadband light is employed, reduced speckle contrast and strong background signals significantly impair the performance of conventional phase retrieval methods. To overcome this limitation, we introduce an adaptive central moment descent (ACMD) method, which enhances speckle contrast via high-order central moment processing.

Extracellular vesicle manufacture via FACTORY: fully automated collection technology and optimum machinery for clinical translational applications.

Extracellular vesicles (EVs) hold significant potential as therapeutic agents and drug carriers. However, current isolation techniques severely limit their clinical application, due to heavy reliance on manual operation, making large-scale isolation of EVs impractical and failing to meet the requirements for clinical translation. Here, we set up the fully automated collection technology and optimum machinery (FACTORY) platform, allowing the efficient collection of high-quality EVs.

Tartrazine-enabled optical clearing for in vivo optical resolution photoacoustic microscopy.

Optical-resolution photoacoustic microscopy (OR-PAM) is a powerful imaging technique that visualizes microvascular and tissue structures with high spatial resolution, offering valuable insights into physiological and pathological processes. However, strong optical scattering in biological tissues fundamentally limits its imaging depth. Recent studies have suggested that tartrazine, a food-grade water-soluble dye, may serve as an effective optical clearing agent (OCA), yet its strong optical absorption near 532 nm raises concerns about compatibility with green-light-based OR-PAM systems.

Engineered EVs from 3D-Cultured MSCs for Synergistic Modulation of Inflammatory Microenvironment and Cartilage Regeneration in Osteoarthritis.

Osteoarthritis (OA) is a common and serious joint disease characterized by synovitis and articular cartilage degeneration. Effective nonsurgical treatments for OA are still lacking. In this study, we designed an injectable temperature-sensitive hydrogel system delivering engineered extracellular vesicles for the treatment of OA.

The Crosstalk Between Sepsis-Associated Encephalopathy and Alzheimer's Disease: Identifying Potential Biomarkers and Therapeutic Targets for Cognition.

Patients with sepsis are at a heightened risk of long-term cognitive impairment, including neurodegenerative diseases; however, the underlying pathophysiological mechanisms remain incompletely understood. This study examines key genes associated with sepsis and Alzheimer's disease (AD), as well as their potential molecular mechanisms. We downloaded the GSE135838 dataset from the Gene Expression Omnibus (GEO) database and performed comparative analysis of differentially expressed genes (DEGs) using the AlzData database to identify co-expressed DEGs.

Peptide-Enhanced Bioactive Hydrogel Combined with Photodynamic-Phage Synergistic Antibacterial Therapy System Accelerates Infected Wound Healing.

Bacterial resistance poses a serious clinical challenge. This study designs a peptide-enhanced bioactive hydrogel with a photodynamic-phage synergistic antibacterial effect that is an efficient antibacterial and promotes wound healing. LaFeO@CN is characterized with XRD, XPS, SEM, and UV-vis, and the photodynamic properties of LaFeO@CN producing ROS are confirmed by EPR.

LAG3 limits regulatory T cell proliferation in α-synuclein gut-to-brain transmission model.

Background: Pathological α-synuclein (α-syn) can spread from the gut to the central nervous system (CNS), with CD4 + T cells playing a key role in this process. Lymphocyte activation gene 3 (LAG3) is involved in intestinal inflammation, regulates CD4 + T cell proliferation and function, and can specifically bind to pathological α-syn during cell-to-cell transmission. However, it remains unclear whether LAG3 is involved in the spread of pathological α-syn from the gut to the brain.

ATP6V0A1 protects dopaminergic neurons via the autophagy-lysosomal pathway in Parkinson's disease.

Parkinson's disease is the second most common neurodegenerative disorder. ATPase H + transporting V0 subunit A1 (ATP6V0A1) is a component of vacuolar H + -ATPase (V-ATPase), an ATP-dependent proton pump. Our previous research identified an association between the ATP6V0A1 rs601999 variant and Parkinson's disease; however, the underlying mechanisms of ATP6V0A1 in Parkinson's disease remain elusive.

Upregulation of PD-1 on Peripheral T cells Subsets is Associated with Parkinson's Disease.

Parkinson's disease (PD) is the second most prevalent neurodegenerative disease, and previous research has shown that T cell-mediated immunity plays a key role in PD pathology. Programmed cell death protein 1 (PD-1) is a type I transmembrane protein that inhibits T lymphocyte inflammatory activity, and PD-1 deletion can reduce dopamine levels in mouse brains. However, the clinical status of PD-1 in PD patients remains obscure.

KLF7-regulated ITGA2 as a therapeutic target for inhibiting oral cancer stem cells.

Cancer stem cells (CSCs) play crucial roles in tumor metastasis, therapy resistance, and immune evasion. Identifying and understanding the factors that regulate the stemness of tumor cells presents promising opportunities for developing effective therapeutic strategies. In this study on oral squamous cell carcinoma (OSCC), we confirmed the key role of KLF7 in maintaining the stemness of OSCC.

AARS2-catalyzed lactylation induces follicle development and premature ovarian insufficiency.

Lactate, a metabolite which is elevated in various developmental and pathological processes, exerts its signal through alanyl tRNA synthetases (AARS)-catalyzed protein lactylation. Herein, we report that elevated lactate and gain-of-function mitochondrial AARS (AARS2) mutations-induced hyper-lactylation promotes premature ovarian insufficiency (POI). Serum lactate is elevated in POI patients.

Anterior mandibular distraction osteogenesis: a retrospective study of condylar stability in the treatment of mandibular retrognathia secondary to idiopathic condylar resorption.

Introduction: The etiology of idiopathic condylar resorption (ICR) is not conclusively established, and its treatment is primarily focused on correcting secondary maxillofacial deformities. Condylar resorption is a common complication of the treatment of ICR. As one treatment for mandibular retrognathia secondary to idiopathic condylar resorption (MRSICR), distraction osteogenesis (DO) can improve the facial profile and the occlusion.

A machine learning model for predicting severe mycoplasma pneumoniae pneumonia in school-aged children.

Objective: To develop an interpretable machine learning (ML) model for predicting severe Mycoplasma pneumoniae pneumonia (SMPP) in order to provide reliable factors for predicting the clinical type of the disease.

Methods: We collected clinical data from 483 school-aged children with M. pneumoniae pneumonia (MPP) who were hospitalized at the Children's Hospital of Soochow University between September 2021 and June 2024.

Scalable fabrication of nano-to-micro carbon disk ultramicroelectrodes for single small extracellular vesicle detection.

Small extracellular vesicles (sEVs) play a crucial role in intercellular communication, but their nanoscale size and heterogeneity make analysis challenging. This study introduces a scalable method for fabricating disk carbon fiber ultramicroelectrodes (UMEs) with precise size control. Size-matched UMEs enable single-sEV detection blocking collisions, achieving a high signal-to-background ratio and low noise.

An electrochemical fluorescence dual-mode strategy for HER2-positive breast cancer cell detection.

Breast cancer is one of the most prevalent malignancies worldwide, with HER2-overexpressing subtypes exhibiting increased aggressiveness and poorer prognosis. Accurate identification of HER2-positive subtypes is essential for the effective implementation of HER2-targeted therapy. In this study, an electrochemical fluorescence dual-mode strategy was developed for the high sensitive detection of HER2-positive breast cancer cells.

Enhanced catalytic efficiency of nanozymes with a V-structured chip for microfluidic biosensing of .

Nanozymes, nanomaterials with enzyme-like characteristics which exhibit lower cost, easier synthesis and functionalization, and better stability compared with natural enzymes, have been widely developed for biosensing, disease therapy and environmental governance. However, the lack of catalytic efficiency of nanozymes compared to natural enzymes makes it difficult for them to completely replace natural enzymes to achieve higher sensitivity and lower detection limits in biosensing. Herein, magnetism-controlled technology was used to form a nanozyme array consisting of stacked FeO/Au NPs at the bottom of the microchannel as a spatially confined microreactor for the catalytic reaction.

Renal parenchymal volume analysis: Clinical and research applications.

Background And Objectives: In most patients, the renal parenchymal volumes in each kidney directly correlate with function and can be used as a proxy to determine split renal function (SRF). This simple principle forms the basis for parenchymal volume analysis (PVA) with semiautomated software, which can be leveraged to predict SRF and new-baseline glomerular filtration rate (NBGFR) following nephrectomy. PVA was originally used to evaluate renal transplantation donors and has replaced nuclear renal scans (NRS) in this domain.

Response evaluation and tumor shrinkage pattern post-SBRT for renal cell carcinoma.

Purpose: Despite the promising application of stereotactic body radiotherapy (SBRT) in renal cell carcinoma (RCC), the optimal time and method for assessing tumor responses to SBRT remain unclear. We aimed to compare the utility of Response Evaluation Criteria in Solid Tumors (RECIST) 1.1 and modified RECIST (mRECIST) in RCC response assessment and clarify the tumor shrinkage pattern post-SBRT.

Nano-Collision Electrochemistry for Real-Time Monitoring of Amyloid-β Oligomerization and Rapid Screening of Degrading Drugs.

Toxic oligomers of amyloid-β (Aβ) are important in the pathology of Alzheimer's disease (AD), and degradation of Aβ oligomers (AβO) in the brain is considered a promising strategy for drug development. However, conventional drug screening techniques face challenges in the rapid and real-time assessment of AβO. Here, we report a simple and reliable nanocollision electrochemical method based on silver nanoparticles (AgNPs) "tagging" that can in situ monitor Aβ oligomerization and screen potential AβO-degrading drugs.