Biomechanical analysis of posterior, transforaminal, extreme, oblique, and anterior lumbar interbody fusion surgical models: a finite element study.

Many lumbar interbody fusion methods have been proposed, but there is a relative scarcity of fundamental biomechanical research on these varied surgical procedures. In this study, a finite element model of an L4-L5 functional spinal unit was created and five interbody fusion methods were evaluated, including posterior lumbar interbody fusion (PLIF), transforaminal lumbar interbody fusion (TLIF), extreme lateral interbody fusion (XLIF), oblique lateral interbody fusion (OLIF) and anterior lumbar interbody fusion (ALIF). Static and harmonic vibration surgery analysis models were developed based on it, investigating the biomechanical properties, as well as the variation of strain energy.

Computational modeling of biomechanical response of osteocyte integrin and cytoskeleton based on the piezoelectricity of bone matrix.

Osteocytes are key in bone remodeling, responding to mechanical stimuli. The piezoelectric bone matrix converts these stimuli into electrical signals, influencing remodeling. To delve deeper into this, we created an osteocyte model within a piezoelectric bone matrix, incorporating the lacuna-canalicular system and mechanosensors such as integrins, cytoskeleton, and primary cilia.

A finite element study of the biomechanics of aging osteocyte model.

Aging lacuna-canalicular system (LCS) has osteocyte size reduction, cell process number loss, and canaliculus blockage. This study built four osteocyte aging models for various aging features. These models have processes, collagen hillocks, and primary cilia mechanoreceptors for signal comparison.

Repeat-dose toxicity study of human umbilical cord mesenchymal stem cells in cynomolgus monkeys by intravenous and subcutaneous injection.

Human umbilical cord mesenchymal stem cells (hUC-MSCs) are proposed for the treatment of acute lung injury and atopic dermatitis. To advance hUC-MSC entry into clinical trials, the effects of hUC-MSCs on the general toxicity, immune perturbation and toxicokinetic study of hUC-MSCs in cynomolgus monkeys were assessed. hUC-MSCs were administered to cynomolgus monkeys by intravenous infusion of 3.

Preclinical Toxicity and Immunogenicity of a COVID-19 Vaccine (ZF2001) in Cynomolgus Monkeys.

Although the new coronavirus disease 2019 (COVID-19) outbreak occurred in late 2019, it is still endemic worldwide, and has become a global public health problem. Vaccination against SARS-CoV-2 is considered to be the most effective intervention to prevent the spread of COVID-19. ZF2001 is a recombinant protein vaccine based on SARS-CoV-2 receptor-binding domain (RBD) subunit which contains aluminum adjuvant.

Root Extract of (Sims) Kosterm. Modulates the Th17/Treg Balance to Attenuate DSS-Induced Colitis in Mice by IL-6/STAT3 Signaling Pathway.

Ulcerative colitis (UC) is a chronic, idiopathic and relapsing inflammatory disease of the gastrointestinal tract that has a prolonged disease duration. (Sims) Kosterm. is a traditional Chinese herb which has been used to treat gastrointestinal diseases for thousand years.

Facile synthesis of red dual-emissive carbon dots for ratiometric fluorescence sensing and cellular imaging.

Recently, widespread attention has been paid to red emissive carbon dots (CDs) which have desirable optical properties, low toxicity, and biocompatibility. Despite great efforts, the facile preparation of red dual-emissive CDs useful for ratiometric detection and bioimaging remains challenging. Here, we report a facile synthesis of red dual-emissive CDs and their potential for ratiometric fluorescence sensing and cellular imaging.

[Occurrence Characteristics and Risk Assessment of Antibiotics in Source Water of the Nanjing Reach of the Yangtze River].

Occurrence characteristics of antibiotics in the source water of the Nanjing section of the Yangtze River were analyzed preliminarily by solid-phase extraction and high-performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS). The results showed that the cumulative concentration of 16 sampling points ranged from 13.37 ng·L to 780.

Application of a DSS colitis model in toxicologically assessing norisoboldine.

In standard nonclinical drug safety evaluation studies, limitations exist in predicting the clinical risk of a drug based only on data from healthy animals. To obtain more comprehensive toxicological information on norisoboldine (NOR), we conducted an exploratory study using C57BL/6 mice in addition to healthy mice as models of dextran sodium sulfate (DSS) colitis to evaluate the safety of NOR. The healthy mice and DSS colitis mice were exposed to 30 or 90 mg NOR/kg body weight or water for 15 days.

[Simultaneous determination of five types of 40 antibiotics in surface water using high performance liquid chromatography-tandem mass spectrometry].

A method was developed for the determination of five categories of 40 antibiotics in surface water by using solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS). The water samples were enriched and cleaned-up by filtration and with SPE cartridges. All antibiotics were separated by gradient elution with the mobile phase of 0.

Combined Antihypertensive Effect of Paeoniflorin Enriched Extract and Metoprolol in Spontaneously Hypertensive Rats.

Background: Hypertension is a great global health challenge and it mostly requires drug combination therapy with the various advantages. Metoprolol (MP) and paeoniflorin are both commonly used for the treatment of hypertension. However, whether they exert synergistic effects on antihypertension or not remains unclear, especially on vascular endothelial function.

Beneficial Effects of Paeoniflorin Enriched Extract on Blood Pressure Variability and Target Organ Damage in Spontaneously Hypertensive Rats.

Blood pressure variability (BPV) is associated with the development and progression of severe target organ damage (TOD). This study aims to evaluate the protective effect of paeoniflorin enriched extract from Radix Paeoniae Alba (PG) on BPV and TOD in spontaneously hypertensive rats (SHR). All SHR were orally treated with distilled water, metoprolol (MP, 20 mg/kg), and PG (PG-H, 90 mg/kg or PG-L, 30 mg/kg) for a single time or daily for 7 weeks.

[Effect of extracts from Dendrobii ifficinalis flos on hyperthyroidism Yin deficiency mice].

Some unhealthy life habits, such as long-term smoking, heavy drinking, sexual overstrain and frequent stay-up could induce the Yin deficiency symptoms of zygomatic red and dysphoria. Stems of Dendrobii officinalis flos (DOF) showed the efficacy of nourishing Yin. In this study, the hyperthyroidism Yin deficiency model was set up to study the yin nourishing effect and action mechanism of DOF, in order to provide the pharmacological basis for developing DOF resources and decreasing resource wastes.

Studies on the effects of Al(III) on the lactate dehydrogenase activity by differential pulse voltammetry.

In this paper, differential-pulse voltammetry (DPV) was applied to study the effects of aluminum (Al(III)) on the lactate dehydrogenase (LDH) activity, K(m)(NADH) and nu(max) in the enzyme promoting catalytic reaction of "Pyruvate+NADH+H(+)right arrow over left arrowLDHLactate+NAD(+)" by monitoring DPV reduction current of NAD(+). The changes of Al's influence on the LDH activities caused by the concentration of LDH, pH, temperature as well as Al speciation including Al hydroxide (Al-OH), Al fluoride (Al-F) and organically complexing Al (Al-Org) compounds have been investigated. The results showed that the effects of Al on the LDH activity exhibited two kinds of behaviors under different conditions, i.

Potentiometric and multinuclear NMR studies on the interaction of aluminum with ascorbic acid in acidic aqueous solution.

The complex-formation equilibria between aluminum(III) ion and L-(+)-ascorbic acid (AA) in 0.1 M KCl ionic medium at 25 degrees C and 0.15 M NaCl ionic medium at 37 degrees C were studied by glass electrode pH-metric measurements.

NMR spectra and potentiometry studies of aluminum(III) binding with coenzyme NAD+ in acidic aqueous solutions.

Complexation and conformational studies of coenzyme NAD+ with aluminum were conducted in acidic aqueous solutions (pH 2-5) by means of potentiometry as well as multinuclear (1H, 13C, 31P, 27Al) and two-dimensional (1H, 1H-NOESY) NMR spectroscopy. These led to the following results: (1) Al could coordinate with NAD+ through the following binding sites: N7' of adenine and pyrophosphate free oxygen (O(A)1, O(N)1,O(A)2) to form various mononuclear 1:1 (AlLH23+, AlLH2+) and 2:1 (AlL2-) species, and dinuclear 2:2 (Al2L22+) species. (2) The conformations of NAD+ and Al-NAD+ depended on the solvents and different species in the complexes.