Timosaponin B- II Enhances Osteogenic Differentiation of Human Periodontal Ligament Stem Cells via PI3K/AKT/GSK3β Signaling Pathway.

Background: This investigation aims to elucidate the effects of Timosaponin B-II (TB-II) on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) through both in vitro experiments and an in vivo orthodontic tooth movement model utilizing rats. The primary objective is to clarify the mechanisms by which TB-II influences the remodeling of periodontal tissue under biomechanical stress, thereby providing insights into its potential role in reducing relapses after orthodontic tooth movement.

Methods: hPDLSCs were isolated and characterized via flow cytometry and multilineage differentiation assays (osteogenic and adipogenic induction).

Astragaloside IV Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells via Activating PI3K/AKT/eNOS/NO Signaling Pathway: In vitro and in vivo Study.

Purpose: Periodontal ligament stem cells (PDLSCs) play a critical role in alveolar bone regeneration and orthodontics. Astragaloside IV (AS-IV) is the chief ingredient of Astragalus, which has been shown to promote osteogenesis. The study aimed to detect the impact of AS-IV on osteogenic differentiation of PDLSCs and to investigate the role of the PI3K/AKT/eNOS/NO pathway in this process.

A higher-order interaction model of susceptible-infected-susceptible epidemics on networks with nonlinear microscopic incidence rate.

This work studies spreading of susceptible-infected-susceptible models on general networks characterized by microscopic nonlinear incidence rates, where the likelihood of a susceptible node becoming infected is expressed as a nonlinear function of the number of its infected neighbors. When the infection function is a general polynomial, we analytically develop a quenched mean-field model, encompassing two different types of higher-order interaction terms. Notably, these higher-order terms exhibit strong similarities to the established simplicial spreading model and the general higher-order spreading model, with the primary distinction lying in the varying coefficients of the involved variables.

OSMlanduse a dataset of European Union land use at 10 m resolution derived from OpenStreetMap and Sentinel-2.

Our map represents the first successful large-area fusion of OpenStreetMap and Copernicus data at a spatial resolution of 10 m or finer and can be applied globally. We addressed varying label noise and feature space quality, utilizing artificial intelligence and advanced computing. Our method relies solely on openly available data streams and methods, eliminating training data acquisition or the need for additional expert knowledge for such purpose.

LINC00673 binds with leptin to regulate osteogenic function in periodontal ligament stem cells.

Background: The aim of this study is to investigate whether LINC00673 and leptin are associated with osteogenesis of periodontal ligament stem cells in the microenvironment of advanced glycation end products.

Methods: LINC00673 expression was detected in PDLSCs by qRT-PCR performed during osteogenic differentiation. By using alkaline phosphatase and Alizarin red S staining, we were able to confirm the role of LINC00673 in regulating osteogenesis in PDLSCs.

Catalpol Enhances Osteogenic Differentiation of Human Periodontal Stem Cells and Modulates Periodontal Tissue Remodeling in an Orthodontic Tooth Movement Rat Model.

Purpose: This study examines the effects and mechanisms of catalpol (CAT) on the proliferation and osteogenic differentiation of cultured human periodontal ligament stem cells (hPDLSCs) in vitro and assesses the impact of CAT on periodontal remodeling in vivo using an orthodontic tooth movement (OTM) model in rats.

Methods: hPDLSCs were cultured in a laboratory setting, and their proliferation and osteogenic differentiation were assessed using the Cell-counting Kit-8 (CCK-8), Alizarin Red Staining (ARS), quantitative calcium assay, alkaline phosphatase (ALP) staining and activity assay, and immunofluorescence assay. Additionally, the expression of collagen type 1 (COL-1), ALP, and runt-related transcription factor-2 (RUNX-2) was evaluated through qRT-PCR and Western blot analysis.

Theory of Lipid Metabolism Disorders in Rhinitis and Asthma (Lipid Droplets).

Lipid droplets are important for the storage of neutral lipids in cells; moreover, they participate in a variety of activities in cells and are multifunctional organelles. In the past few decades, lipid droplets have been extensively studied and found to play important roles in cellular energy balance, signal regulation and metabolic regulation. In particular, the formation and function of lipid droplets in adipocytes and mast cells have received much attention.

Fixed-Time Synchronization of Coupled Oscillator Networks with a Pacemaker.

This paper investigates the fixed-time synchronization problem of a Kuramoto-oscillator network in the presence of a pacemaker. Based on the framework of the cyber-physical system (CPS), fixed-time synchronization criteria of such network are presented respectively for identical and non-identical oscillators. In virtue of Lyapunov stability analyses, sufficient conditions are deduced for achieving phase agreement and frequency synchronization for arbitrary initial phases and/or frequencies under distributed control strategies.

Tetraarsenic hexoxide enhances generation of mitochondrial ROS to promote pyroptosis by inducing the activation of caspase-3/GSDME in triple-negative breast cancer cells.

Although tetraarsenic hexoxide is known to exert an anti-tumor effect by inducing apoptosis in various cancer cells, its effect on other forms of regulated cell death remains unclear. Here, we show that tetraarsenic hexoxide induces the pyroptotic cell death through activation of mitochondrial reactive oxygen species (ROS)-mediated caspase-3/gasdermin E (GSDME) pathway, thereby suppressing tumor growth and metastasis of triple-negative breast cancer (TNBC) cells. Interestingly, tetraarsenic hexoxide-treated TNBC cells exhibited specific pyroptotic characteristics, including cell swelling, balloon-like bubbling, and LDH releases through pore formation in the plasma membrane, eventually suppressing tumor formation and lung metastasis of TNBC cells.

Anti-Cancer Activity of AsO and its Efficacy in a Series of Patient-Derived Xenografts for Human Cervical Cancer.

Purpose: To investigate the anti-cancer effects of tetraarsenic hexoxide (TAO, AsO) in cervical cancer cell lines and in a series of patient-derived xenograft (PDX) mouse models.

Methods: Human cervical cancer cell lines, including HeLa, SiHa and CaSki, and human umbilical vein endothelial cells (HUVECs), were used to evaluate the anti-cancer activity of TAO. Cellular proliferation, apoptosis, and enzyme-linked immunosorbent assay (ELISA) for matrix metallopeptidase 2 (MMP-2) and 9 (MMP-9) were assessed.

Effect of weimaining on apoptosis and Caspase-3 expression in a breast cancer mouse model.

Ethnopharmacological Relevance: Weimaining (WMN) is a condensed Tannin compound extracted from Fagopyrum cymosum (Trevir.) Meisn., which comes from the roots of buckwheat, a type of Chinese herbal medicine, was first recorded in "Bencao Shiyi".

Niu-Huang-Shen suppresses hepatocellular carcinoma cell growth and metastasis by regulating Yap1 expression.

Hepatocellular carcinoma (HCC) is one of the most common cancers types. Niu-Huang-Shen (NHS), a Chinese medicine, has been reported to exert antipyretic, anti-inflammatory and vasodilatation effects. However, whether NHS has inhibitory effects on HCC cell phenotypes has remained elusive.

Neuroprotective effects of autophagy induced by rapamycin in rat acute spinal cord injury model.

Background/aims: To explore the effects of rapamycin-induced autophagy on apoptosis in a rat model of acute spinal cord injury (SCI), and to explore the effect of rapamycin on apoptosis in primary spinal cord cell culture.

Methods: SCI was induced at T10 in female adult Sprague-Dawley rats. After injury was induced, the rats were injected with rapamycin and/or methylprednisolone and were sacrificed at various days after injury.

Adaptive synchronization and pinning control of colored networks.

A colored network model, corresponding to a colored graph in mathematics, is used for describing the complexity of some inter-connected physical systems. A colored network is consisted of colored nodes and edges. Colored nodes may have identical or nonidentical local dynamics.

Synchronization of a network coupled with complex-variable chaotic systems.

In this paper, synchronization of a network coupled with complex-variable chaotic systems is investigated. Adaptive feedback control and intermittent control schemes are adopted for achieving adaptive synchronization and exponential synchronization, respectively. Several synchronization criteria are established.

[Effects of multi-nutrients supplementation on the nutritional status and antioxidant capability of healthy adults].

Objective: To determine whether daily multivitamins/minerals supplement can improve nutrient status, plasma, antioxidant enzymes activity and total antioxidant capacity in healthy adults.

Methods: One hundred and fifty-one healthy adults living in a normal lifestyle with a mean age of 28 (20 - 50) years were recruited from Guangzhou. The subjects were divided into the supplement group and the control ineral supplements.