Ultra-sensitive solid-state nanochannel electrochemical biosensor based on interfacial charge density modulation and signal synergistic amplification for microRNA-155 detection.

Solid-state nanochannel sensors have emerged as a promising platform for next-generation disease marker detection. However, significant challenges remain in detecting low-abundance miRNAs in biological systems. This study presents an ultra-sensitive electrochemical sensing system based on interfacial charge density modulation and signal synergistic amplification within nanochannel for precise detection of microRNA-155 (miR-155).

Metabolic and hepatic biomarkers associated with MASLD in the Chinese population.

Metabolic dysfunction-associated steatotic liver disease (MASLD) has become the most prevalent chronic liver disease globally, imposing significant economic burdens. In this study, we analyzed serum indicators-including ALT, AST, De-Ritis ratio, fasting blood glucose (FBG), lipid profiles, and blood pressure-among 162,699 Chinese individuals from Ping An Healthcare Diagnostics Center, stratified by gender, age, and weight. Comparative analysis revealed significant differences in these biomarkers between MASLD and Normal groups, enabling the identification of markers associated with MASLD subhealth.

Multi-omics analysis to a sludge composting system reveals thermophilic bacteria promote free amino acid production and compost quality.

Recycling waste nutrients from waste activated sludge (WAS) back to agricultural land through composting embodies the core principle of circular economy. However, large quantities of nitrogen are lost as nitrogenous gases during composting, reducing the fertilization performance of the final products. Here, the N cycle and amino acid (AA) biotransformation profiles in an industrial-scale sludge composting plant (130t/d) were investigated using replicated and temporally sampled multi-omics datasets.

Novel classification for microspherophakia and its related surgical implications: a retrospective cohort study.

Background: A novel classification for microspherophakia (MSP) was developed using a nomogram model to stratify patients and evaluate surgical outcomes.

Methods: The study involved 99 eyes from 64 MSP patients, divided into training (64 eyes) and validation (35 eyes) cohorts. Primary outcome included the best corrected distance visual acuity (BCVA) at the 1-year follow-up, and the secondary outcome was intraocular pressure (IOP) control.

Longitudinal Observation of Posterior Capsule Opacification in Pediatric Eyes with Marfan Syndrome after In-the-bag Intraocular Lens Implantation.

Purpose: To identify risk factors for posterior capsule opacification (PCO) after in-the-bag intraocular lens (IOL) implantation in pediatric patients with Marfan syndrome (MFS) and ectopia lentis (EL).

Setting: Eye & ENT Hospital of Fudan University, Shanghai, China.

Design: Prospective cohort study.

Retinal layer thinning as a predictor of demyelinating diseases of the central nervous system: Insights from a decade-long cohort study.

Background: Early diagnosis and differentiation of demyelinating diseases of the central nervous system (CNS) are essential for timely treatment but challenged with the limited availability of non-invasive, real-time methods to assess the architecture of the CNS. This study compared the retinal layers between patients with CNS demyelinating diseases and the general population and evaluated the predictive value of these structures in prospective diagnosis.

Methods: This UK Biobank study, incorporating optical coherence tomography images, analyzed patients with CNS demyelinating diseases identified at recruitment and during follow-up.

Accuracy of Intraocular Lens Power Calculation in Microspherophakia Patients: Comparison of 7 Formulas.

Introduction: The purpose of this study was to compare the accuracy of seven formulas for intraocular lens power calculation in patients with microspherophakia (MSP).

Methods: A retrospective case series included 44 eyes from 28 patients with MSP. The mean prediction error (PE) was calculated, and the accuracy was determined by formula performance index (FPI), median absolute error (MedAE), and percentage of eyes with a PE within ±0.

Corneal Biomechanics Are Associated With FBN1 Mutations in Patients With Marfan Syndrome and Ectopia Lentis.

Background: We investigated the corneal biomechanical properties and their genotype-phenotype correlation correlations in patients with Marfan syndrome (MFS) and ectopia lentis (EL).

Methods: Patients with MFS with EL underwent panel-based next-generation sequencing in this retrospective cohort study. The FBN1 genotypes were categorized into the dominant-negative (DN) group and the haploinsufficiency (HI) group.

Advanced Artificial-Intelligence-Based Jiang Formula for Intraocular Lens Power in Congenital Ectopia Lentis.

Purpose: The purpose of this study was to develop an artificial intelligence (AI)-based intraocular lens (IOLs) power calculation formula for improving the accuracy of IOLs power calculations in patients with congenital ectopia lentis (CEL).

Methods: A total of 651 eyes with CEL that underwent IOLs implantation surgery were included in this study. An AI-based ensemble formula-the Jiang Formula, was developed using a training dataset of 520 eyes and evaluated on a testing dataset of 131 eyes.

Corrigendum: Clinical ocular prediction model of postoperative ametropic amblyopia in patients with congenital ectopia lentis.

[This corrects the article DOI: 10.3389/fmed.2024.

Modular Access to Silicon-Containing Amino Acids and Peptides by Cobalt Catalysis.

A regioselective cobalt-catalyzed three-component silylamidation that rapidly and reliably incorporates dioxazolones and silylzinc pivalates into unconjugated alkenyl amides is disclosed. Notably, the unique power of this protocol is demonstrated by the possibility of achieving peptide ligation using peptide-containing dioxazolones or alkenyl amides as the coupling partners. Moreover, this approach is distinguished by its mild condition, synthetic simplicity, and ample scope, thus providing a new platform for modular access to silicon-containing amino acid derivatives and peptides.

Reprogramming of iPSCs to NPCEC-like cells by biomimetic scaffolds for zonular fiber reconstruction.

Ectopia lentis (EL), characterised by impaired zonular fibers originating from non-pigmented ciliary epithelial cells (NPCEC), presents formidable surgical complexities and potential risks of visual impairment. Cataract surgery is the only treatment method for EL, but it leads to the loss of accommodative power of the lens post-operatively. Furthermore, the challenge of repairing zonular ligaments remains a significant global issue.

Improving the accuracy of lens formulas for in-the-bag intraocular lens implantation in Marfan syndrome patients with ectopia lentis.

Purpose: To improve the accuracy of intraocular lens (IOL) power calculation formulas by modifying the effective lens position (ELP) equations for patients with Marfan syndrome (MFS) and ectopia lentis (EL) undergoing in-the-bag IOL implantation.

Setting: Eye and ENT Hospital of Fudan University, Shanghai, China.

Design: Retrospective cohort study.

lncRNA LINC02323 predicts adverse neoadjuvant chemotherapy outcomes of gastric cancer patients and regulates cell sensitivity to 5-fluorouracil by negatively modulating miR-139-3p.

Background/objective: Drug resistance is a challenging problem in the clinical chemotherapy of gastric cancer. Identification of predictive biomarkers for chemotherapy outcomes could improve therapeutic efficacy and patient prognosis. This study aimed to assess the significance of long non-coding RNA (lncRNA) LINC02323 in gastric cancer progression and neoadjuvant chemotherapy and to explore its potential regulatory mechanism.

Clinical ocular prediction model of postoperative ametropic amblyopia in patients with congenital ectopia lentis.

Introduction: Despite prompt and appropriate surgical management, a considerable proportion of patients with congenital ectopia lentis (CEL) suffer from postoperative ametropic amblyopia. To predict and identify at-risk patients early, and ensure timely amblyopia treatment, we conducted a thorough investigation into the onset and progression patterns of postoperative amblyopia in patients with CEL. Moreover, an ocular prediction model was constructed for amblyopia.

Revealing the interplay of dissolved organic matters variation with microbial symbiotic network in lime-treated sludge landscaping.

Lime pretreatment is commonly used for sludge hygienization. Appropriate lime dosage is crucial for achieving both sludge stabilization (lime dosage >0.2 g/g-TS) and promoting plant and soil health during subsequent landscaping (lime dosage <0.

Spatial and temporal evolution patterns and driving mechanisms of rural settlements: a case study of Xunwu County, Jiangxi Province, China.

Urbanization and industrialization have driven rapid socio-economic development, leading to a significant population shift from rural areas to cities. This demographic transition has resulted in substantial changes in rural settlements, presenting considerable challenges for rural revitalization. Given the spatial differentiation, it is crucial to analyze the spatiotemporal evolution patterns and driving mechanisms of rural settlements under various development scenarios and at multiple scales within a county.

Genotype Associated With Visual Prognosis in Patients With Congenital Ectopia Lentis Following Lens Surgery: A Prospective Cohort Study.

Purpose: To investigate the relationship between visual prognosis and genotype in patients undergoing lens surgery for congenital ectopia lentis (EL).

Design: Prospective clinical cohort study.

Methods: Patients with congenital EL who underwent lens removal and intraocular lens implantation received panel-based next-generation sequencing.

ErbB4 deficiency exacerbates olfactory dysfunction in an early-stage Alzheimer's disease mouse model.

Olfactory dysfunction is increasingly recognized as an early indicator of Alzheimer's disease (AD). Aberrations in GABAergic function and the excitatory/inhibitory (E/I) balance within the olfactory bulb (OB) have been implicated in olfactory impairment during the initial stages of AD. While the neuregulin 1 (NRG1)/ErbB4 signaling pathway is known to regulate GABAergic transmission in the brain and is associated with various neuropsychiatric disorders, its specific role in early AD-related olfactory impairment remains incompletely understood.

Genotype-phenotype profile of global ASPH-associated ectopia lentis and clinical findings from a Chinese cohort.

Background: Traboulsi syndrome is an under-recognized syndromic form of ectopia lentis (EL) caused by the aspartate beta-Hydroxylase (ASPH) variant. The genotype-phenotype profile of ASPH-associated disease is poorly understood due to the rarity of the condition.

Methods: We conducted targeted next-generation sequencing and bioinformatics analysis to identify potentially pathogenic ASPH variants in the cohort.

Molecular insights into effects of chemical conditioning on dissolved organic phosphorus transformation and bioavailability during sludge composting.

Phosphorus is enriched in waste activated sludge (WAS) during wastewater treatment, and organic phosphorus (OP) is a potential slow-release P fertilizer. The chemical coagulants used in sludge dewatering leave numerous residues in WAS that affect sludge composting. In this study, the effects of polyaluminum chloride (PAC) and polyferric sulfate (PFS) on the bioconversion of dissolved OP (DOP) during sludge composting were investigated.

Gut microbiome-mediated monocytes promote liver metastasis.

The gut microbiome plays an important role in tumor growth by regulating immune cell function. However, the role of the gut microbiome-mediated monocytes in liver metastasis remains unclear. In this study, we found that fecal microbiome transplantation (FMT) from the stool of patients with liver metastasis (LM) significantly promoted liver metastasis compared with healthy donors (HD).

Influencing factors of effective lens position in patients with Marfan syndrome and ectopia lentis.

Aims: The aim of this study was to analyse the effective lens position (ELP) in patients with Marfan syndrome (MFS) and ectopia lentis (EL).

Methods: Patients with MFS undergoing lens removal and primary intraocular lens (IOL) implantation were enrolled in the study. The back-calculated ELP was obtained with the vergence formula and compared with the theoretical ELPs.