UPP1 is a dual biomarker of prognosis and immune microenvironment in IDH wild-type glioblastoma.

Glioblastoma multiforme (GBM) is the most common and aggressive malignant primary brain tumor. Current therapies (temozolomide/radiotherapy) often encounter resistance, necessitating novel molecular targets. Bioinformatics analysis was performed for the data obtained from TCGA, COSMIC, cbioPortal, and MethSurv databases.

Prognostic value of LncRNA DLGAP1-AS2 in bladder cancer and its regulatory effect on tumor progression.

Background: Bladder cancer (BLCA) represents one of the most prevalent malignant neoplasms within the urinary system, and its incidence is progressively rising annually in China.

Objectives: This research investigated the clinical significance of DLGAP1-AS2, and examined its potential mechanisms in modulating the behavior of BLCA cells.

Methods: This study collected cancerous and normal tissue samples from 86 BLCA patients.

Identification of IgE Epitopes and Analysis of Critical Amino Acid Residues in Fish Parvalbumin.

The increasing incidence of fish allergy, primarily triggered by parvalbumin (PV), has become a significant public health issue. Although novel PVs are increasingly being characterized, the allergenic epitopes have not been comprehensively identified. This study aimed to explore the IgE epitopes of PV and identify critical amino acid residues, while systematically assessing their connection to the IgE-binding capacity.

Qidong Huoxue decoction protects against acute lung injury by promoting SIRT1-mediated p300 deacetylation.

Background: Qidong Huoxue Decoction (QD), a traditional Chinese medicine, has been used clinically to treat acute lung injury (ALI); however, its anti-inflammatory mechanisms remain poorly understood.

Purpose: This study aimed to elucidate the mechanism by which QD mitigates ALI, focusing on its anti-inflammatory effects.

Methods: A combination of serum pharmacochemistry, acetyl-proteomic sequencing, and experimental validation was used to investigate the therapeutic effects of QD on ALI.

Acupuncture blocks the STAT3-MMP-2 pathway in a guinea pig model of lens-induced myopia.

Objective: To investigate the effects of manual acupuncture (MA) on the signal transduction and activator of transcription (STAT)3 - matrix metalloproteinase (MMP)-2 pathway in a guinea pig model of lens-induced myopia (LIM).

Methods: A total of 36 male pigmented guinea pigs (aged 2 weeks) were randomly assigned to the normal control (NC) (n = 12), LIM (n = 12) and LIM + MA (n = 12) groups. The NC group received no intervention.

Perioperative recognition and management of rare irrigation-related complications in unilateral biportal endoscopy under general anesthesia: two case reports.

Background: Unilateral biportal endoscopy (UBE) is a widely used minimally invasive technique for treating lumbar disc herniation, employing continuous fluid infusion to maintain a clear surgical field. However, complications related to fluid infusion can occur rarely during or after the procedure, presenting significant challenges in perioperative management.

Case Presentations: Here, we report two cases of postoperative irrigation-related complications.

Case Report: Two cases of atypical neurofibroma neoplasm of uncertain biological potential in the nasal cavity and a literature review.

Objective: This study aims to investigate the clinical diagnosis, treatment processes, and pathological characteristics of two cases of atypical neurofibroma neoplasm of uncertain biological potential (ANNUBP) located in the nasal cavity.

Materials And Methods: We retrospectively analyzed the medical history, imaging studies, pathological results, and follow-up data of two patients diagnosed with nasal ANNUBP who were admitted to Qingdao University Hospital between June and October 2023. A review of relevant literature was conducted to summarize their clinical characteristics, diagnosis, treatment, and prognosis.

Altered Default Mode Network Connectivity in Mild Cognitive Impairment: Insights from Resting-State fMRI Studies.

Mild Cognitive Impairment (MCI) is marked by a measurable decline in cognitive function that exceeds typical age-related changes but does not yet qualify as dementia. The brain's Default Mode Network (DMN) remains active during rest and plays a crucial role in introspective processes, such as memory retrieval and self-referential thinking. Resting-state functional magnetic resonance imaging (rs-fMRI) is a non-invasive neuroimaging technique that measures spontaneous fluctuations in blood oxygenation, providing insights into functional connectivity within brain networks.

Gold Cluster-Based Profiling of Early Stage Acute Kidney Injury through Second Near-Infrared Fluorescence Imaging and Mass Spectrometry Imaging.

Acute kidney injury (AKI) poses a major challenge due to the rapid deterioration of renal function, for which the optimal intervention timing has already passed until clinical diagnostics sense abnormalities. Meanwhile, the lack of means to visually understand early-stage AKI at the molecular level hinders the development of interventive strategies. Herein, we developed a high-orthogonal dual-mode imaging strategy integrating second near-infrared (NIR-II) fluorescence imaging and mass spectrometry imaging (MSI) based on gold clusters for profiling early-stage AKI.

Efficient face information encryption and verification scheme based on full homomorphic encryption.

Emerging global privacy mandates enforce strict requirements for biometric data protection, requiring encrypted processing throughout storage and computation phases. While full homomorphic encryption (FHE)-based face recognition ensures security on platforms like Elastic Compute Service (ECS), three critical compliance gaps persist: 1) about 500× ciphertext expansion in high-dimensional facial features, exceeding practical deployment thresholds, 2) vulnerabilities in live verification scenarios documented by ENISA (83% failure rate in 2024 audits), and 3) absence of mechanisms satisfying IND-CPA and IND-CCA2 standards. These limitations collectively undermine both regulatory adherence and operational efficiency in practical biometric systems.

LncRNA LINC01128 promotes prostate cancer cell proliferation, metastasis, and epithelial-mesenchymal transition by modulating miR-27b-3p.

Background: Prostate cancer (PCa) is a prevalent malignancy within the male reproductive system that poses a significant threat to patients' lives. The function of long non-coding RNA LINC01128 in PCa progression remains to be elucidated.

Objective: The objective was to evaluate the significance of LINC01128 in PCa and to elucidate the underlying mechanisms, thereby identifying a potential target for PCa treatment.

Investigating the influence of myofibrillar protein and chitosan interfacial distribution on the macroscopic characteristics of emulsions.

Myofibrillar protein (MP) and chitosan (CS) complexes, formed through electrostatic interactions, hold promise for creating novel emulsions. This study examines the emulsion system utilizing MP and CS as interfacial phases, exploring the distribution patterns of MP-CS (external MP), CS-MP (external CS), and pre-compounded MP/CS, along with varying MP-to-CS concentration ratios (5:1 to 1:5). MP/CS emulsions exhibited finer particle size and higher zeta potential compared to MP-CS and CS-MP bilayer emulsions, with the 1:1 ratio displaying optimal stability.

A dominant role of transcriptional regulation during the evolution of C photosynthesis in Flaveria species.

C photosynthesis exemplifies convergent evolution of complex traits. Herein, we construct chromosome-scale genome assemblies and perform multi-omics analysis for five Flaveria species, which represent evolutionary stages from C to C photosynthesis. Chromosome-scale genome sequence analyses reveal a gradual increase in genome size during the evolution of C photosynthesis attributed to the expansion of transposable elements.

Buckwheat protein-derived peptide ameliorates insulin resistance by directing O-linked N-acetylglucosamine transferase to regulate the SIRT1/PGC1α pathway.

The antidiabetic activity of the novel Buckwheat protein-derived peptide (Ala-Phe-Tyr-Arg-Trp, AFYRW) and its associated protein glycosylation have been verified. Our preliminary study demonstrates the potential of AFYRW as a therapeutic agent for diabetes, but the mechanism needs further investigation. Given the vital role of O-linked N-acetylglucosamine transferase (OGT) in diabetes mellitus and insulin resistance (IR), we focused on the underlying molecular mechanisms of them in ameliorating IR.

Targeting EPHB2/ABL1 restores antitumor immunity in preclinical models of ependymoma.

Ependymoma (EPN) is a common form of brain tumor in children, often resistant to available cytotoxic therapies. Molecular profiling studies have led to a better understanding of EPN subtypes and revealed a critical role of oncogenes ZFTA-RELA fusion and EPHB2 in supratentorial ependymoma (ST-EPN). However, the immune system's role in tumor progression and response to therapy remains poorly understood.

Leaching behavior and stabilization/solidification characterization of heavy metals from silicomanganese slag.

The utilization of silicomanganese slag (SMS) for the partial substitution of cement holds significant importance in handling environmental risks and achieving the harmless and resourceful utilization of industrial solid wastes. Nevertheless, an in-depth analysis of the leaching behaviors of heavy metals and the solidification/stabilization mechanisms in SMS and cementitious materials is still lacking. In this study, we adopted the toxicity characteristic leaching procedure and horizontal vibration method to simulate the natural leaching environment, thereby exploring the leaching risks related to heavy metals in the aforementioned materials.

Hysteroscopy improves surgical outcomes of hydatidiform moles while reducing postoperative residuals.

Objective: To evaluate the clinical efficacy of hysteroscopy in the treatment of molar pregnancy and postoperative residual tissue.

Methods: This retrospective study involved 68 patients who underwent treatment for molar pregnancy in Shanxi Children's Hospital Shanxi Maternal and Child Health Hospital from April 2020 to May 2022. Based on intervention methods, patients were divided into a conventional group (n=33) and a hysteroscopy group (n=35).

Deciphering the molecular landscape of rheumatoid arthritis offers new insights into the stratified treatment for the condition.

Background: For Rheumatoid Arthritis (RA), a long-term chronic illness, it is essential to identify and describe patient subtypes with comparable goal status and molecular biomarkers. This study aims to develop and validate a new subtyping scheme that integrates genome-scale transcriptomic profiles of RA peripheral blood genes, providing a fresh perspective for stratified treatments.

Methods: We utilized independent microarray datasets of RA peripheral blood mononuclear cells (PBMCs).

Identification of novel drug targets for multiple sclerosis by integrating plasma genetics and proteomes.

Background: Genome-wide association studies (GWAS) have revealed numerous loci associated with multiple sclerosis (MS). However, the challenge lies in deciphering the mechanisms by which these loci influence the target traits. Here, we employed an integrative analytical pipeline to efficiently transform genetic associations to identify novel proteins for MS.

Ophiopogonin D alleviates acute lung injury by regulating inflammation via the STAT3/A20/ASK1 axis.

Background: Acute lung injury (ALI) is characterized by acute pulmonary inflammatory infiltration. Alveolar epithelial cells (AECs) release numerous pro-inflammatory cytokines, which result in the pathological changes seen in ALI. Ophiopogonin D (OD), extracted from the roots of Ophiopogon japonicus (Thunb.

Mass Spectrometry Imaging Reveals the Morphology-Dependent Toxicological Effects of Nanosilvers on Multiple Organs of Adult Zebrafish ().

Nanosilvers with multifarious morphologies have been extensively used in many fields, but their morphology-dependent toxicity toward nontarget aquatic organisms remains largely unclear. Herein, we used matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to investigate the toxicological effects of silver nanomaterials with various morphologies on spatially resolved lipid profiles within multiple organs in adult zebrafish, especially for the gill, liver, and intestine. Integrated with histopathology, enzyme activity, accumulated Ag contents and amounts, as well as MSI results, we found that nanosilvers exhibit morphology-dependent nanotoxicity by disrupting lipid levels and producing oxidative stress.