Ultrasound-assisted gram-scale synthesis of nano-tubular ag-MOF and its hybridization with yellow/green carbon quantum dots: membrane-based turn-on fluorescence recognition for procaterol.

Procaterol, a third-generation long-acting β₂-adrenergic receptor agonist, is widely used in asthma and chronic obstructive pulmonary disease (COPD) treatment. Rapid and sensitive detection of Procaterol is essential for both clinical monitoring and environmental surveillance. This study presents a facile and gram-scale synthesis of tubular silver-based Metal-organic framework (MOF) MONT by employing an ultrasonic-assisted green synthesis strategy (20 kHz ultrasound, 100 W power, 30 min).

Comparative Transcriptomic and Metabolomic Analyses Reveal the Mechanism by Which Foam Macrophages Restrict Survival of Intracellular .

Objectives: This study aimed to investigate the impact of foam macrophages (FMs) on the intracellular survival of (MTB) and identify the molecular mechanisms influencing MTB survival.

Methods: An FM model was established using oleic acid induction. Transcriptomic and metabolomic analyses were conducted to identify the key molecular pathways involved in FM-mediated MTB survival.

Survey and analysis of the prevalence of tobacco use among patients with severe mental illness at a tertiary specialized psychiatric medical center in China.

Background: In China, the coexistence of mental illness and tobacco dependence is a major public health issue. With around 300 million smokers and over one million annual smoking-related deaths, the resulting social and economic burdens are considerable.

Methods: A cross-sectional study design was employed to gather data on tobacco use, readiness to quit smoking, and nicotine dependence from a random sample of 738 patients diagnosed with severe mental illness.

Surface Charge Affects the Intracellular Fate and Clearance Dynamics of CdSe/ZnS Quantum Dots in Macrophages.

The biological effects of nanoparticles are closely related to their intracellular content and location, both of which are influenced by various factors. This study investigates the effects of surface charge on the uptake, intracellular distribution, and exocytosis of CdSe/ZnS quantum dots (QDs) in Raw264.7 macrophages.

An open-source interactive AI framework for assisting automatic literature review in forensic medicine: Focus on brain injury mechanisms.

Background And Objective: Systematic reviews and meta-analyses are critical in forensic medicine; however, these processes are labor-intensive and time-consuming. ASReview, an open-source machine learning framework, has demonstrated potential to improve the efficiency and transparency of systematic reviews in other disciplines. Nevertheless, its applicability to forensic medicine remains unexplored.

Development and validation of a nomogram model to predict postoperative delirium after resection of esophageal cancer.

The study aimed to establish and validate a nomogram model to predict postoperative delirium (POD) among esophageal cancer resection patients. Clinical data of 396 patients with esophageal cancer who underwent esophagectomy from November 2020 to June 2023 in the electronic medical records of cardiothoracic Surgery, Affiliated Hospital of Jiangnan University. Participants were randomly divided into training and testing sets in a 7:3 ratio.

Biodegradable hollow MnO decorated by carbon dots with cholesterol depletion capability for cascaded amplification of sono-immunotherapy.

For the effective activation of the adaptive immune response, it is crucial to promote the maturation of dendritic cells (DCs) and subsequently initiate cytotoxic T lymphocytes. Nevertheless, the immunosuppressive tumor microenvironments (TME) are believed to hinder DC maturation, leading to a significant decrease in the effectiveness of immunotherapy. In this work, we present the first instance of combining ROS-triggered immunogenic cell death (ICD), cholesterol depletion, and STING activation to achieve the cascade amplification of antitumor immune response.

Predicting the ferroptosis-associated gene targets in atherosclerosis by integrating GWAS and eQTL studies summary data.

Atherosclerosis is a chronic, low-grade inflammatory disease affecting the arteries, which causes cardiovascular disease by narrowing the patient's arterial blood vessels, and is currently the number 1 disease killer in the United States. Nevertheless, developing new animal model approaches and novel therapeutic strategies requires time to treat affected individuals who do not benefit from statins. However, the exact mechanism behind AS pathology is still unknown.

A novel immunological perspective on female-specific cancers: Exploring the signaling pathways of tertiary lymphoid structures and their clinical applications.

Female-specific cancers, including breast, cervical, and ovarian cancers, are significant contributors to global morbidity and mortality. Despite advancements in conventional treatment modalities, challenges such as recurrence and therapeutic resistance remain prevalent, highlighting the urgent need for novel therapeutic strategies. Tertiary lymphoid structures (TLS), which act as immune microstructures within the tumor microenvironment, have emerged as key regulators of antitumor immunity.

Towards Multispecific Anti-Gp120 Artificial Antibody.

Broadly neutralizing antibodies are potential therapeutic drugs that can recognize and block viral entry. But many viruses, such as human immunodeficiency virus (HIV), mutate rapidly and thus evade therapeutic antibodies. Multispecific antibodies and combinations of different antibodies can effectively suppress immune evasion.

Novel colchicine ethosomes cataplasm for the treatment of acute gouty arthritis.

Gout is a disease caused by the deposition of sodium urate (MSU) crystals in the joints and tissues. Colchicine (COL) has become the first-line drug for the treatment of acute gout due to its low price and efficacy. However, colchicine is highly cytotoxic and oral administration is prone to cause severe adverse effects on the gastrointestinal tract, liver and kidney.

The Status of Medication Literacy in Young Patients With Hypertension and Its Relationship With Medication Adherence.

Background: Previous investigators have shown that low levels of ML were associated with poor medication adherence, but few have explored this relationship in young patients with hypertension.

Objective: In this study, our objective was to analyze the current state of ML in young Chinese patients with hypertension and determine its relationship with medication adherence.

Methods: This was a prospective observational study using data from young patients with hypertension in Jiangsu Province, China.

Regulation of alkyl hydroperoxidase D by AhpdR in the antioxidant enzyme system of Pseudomonas aeruginosa.

An overabundance of reactive oxygen species (ROS) can disrupt the initial redox equilibrium within cells, resulting in metabolic issues, cellular harm, and potentially death. Pseudomonas aeruginosa is a widespread gram-negative environmental pathogen that causes serious infectious diseases in humans. P.

Interactions between polystyrene nanoparticles and human intestinal epithelial Caco-2 cells.

Nanoplastics enter the human body mainly by ingestion through the gastrointestinal tract and thus the uptake and release of nanoplastics in intestinal cells have been studied. However, the fate of nanoplastics in intestinal cells remains poorly understood, particularly how they are exocytosed. Herein, we investigated the uptake, distribution, and exocytosis of nanoplastics in Caco-2 cells using 70 nm red fluorescent polystyrene (R70PS) as a nanoplastic model.

A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest.

Global climate change is characterized by increased extreme temperatures affecting insects at all trophic levels. Zinc finger proteins (ZFPs) are key regulators of gene expression and cell differentiation in eukaryotes, essential for stress resistance in both animals and plants. Using CRISPR/Cas9 for gene deletion, this study predicted and examined the structure of ZFP320 in the diamondback moth () and investigated its function in temperature stress response through a comprehensive age-stage, two-sex life table analysis.

Gold Nanoparticle-Based Artificial Antibodies as Stable Substitutes for Antibodies in the Immunoassay.

Sandwich enzyme-linked immunosorbent assay (ELISA) is a widely used powerful method to detect antigens in complicated environments, due to the high sensitivity and specificity of monoclonal antibodies. Yet, the intrinsic instability of antibodies limits the applications of sandwich ELISA. To overcome the shortcomings of antibodies, we previously demonstrated that a class of gold nanoparticle (AuNP)-based artificial antibody, named goldbody, can be created by "Goldization" technology, i.

Overweight in mediating the association between depression and new-onset diabetes: A population-based research from Health and Retirement Study.

Background: Several studies have suggested a close link between depression, overweight, and new-onset diabetes, particularly among middle-aged and older populations; however, the causal associations remain poorly understood.

Aim: To investigate the role of overweight in mediating the association between depression and new-onset diabetes in middle-aged and older populations.

Methods: Data of 9426 individuals aged ≥ 50 years from the 1998-2016 Health and Retirement Study database were analyzed.

[Clinical observation on treatment of patients with kidney- deficiency type ankylosing spondylitis by using Bushen Tongdu external treatment].

Objectives: To observe and evaluate the effectiveness and safety of Bushen Tongdu (reinforcing the kidney- and regulating the Governor Vessel) external treatment for patients with kidney- deficiency type ankylosing spondylitis (AS).

Methods: Patients with Kidney- deficiency type AS were randomly divided into an observation group(36 cases, 4 cases dropped out) and a control group(36 cases, 3 cases dropped out). The observation group were received the Bushen Tongdu external treatment which was a combination of Ren Du Zhou-Tian moxibustion and acupoint embedding therapy, i.

Intelligent Recognition and Segmentation of Blunt Craniocerebral Injury CT Images Based on DeepLabV3+ Model.

Objectives: To achieve intelligent recognition and segmentation of common craniocerebral injuries (hereinafter referred to as "segmentation") by training convolutional neural network DeepLabV3+ model based on CT images of blunt craniocerebral injury (BCI), and to explore the value of deep learning in automated diagnosis of BCI in forensic medicine.

Methods: A total of 5 486 CT images of BCI from living persons were collected as the training set, validation set and test set for model training and performance evaluation. Another 255 CT images of BCI and 156 normal craniocerebral CT images from living persons were collected as the blind test set to evaluate the ability of the model to segment the five types of craniocerebral injuries including scalp hematoma, skull fracture, epidural hematoma, subdural hematoma, and brain contusion.

A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer.

The tumor microenvironment (TME) is pivotal in non-small cell lung cancer (NSCLC) progression, influencing drug resistance and immune cell behavior through complex ligand-receptor (LR) interactions. This study developed an epithelial LR-related prognostic risk score (LRrisk) to identify biomarkers and targets in NSCLC. We identified twenty epithelial LRs with significant prognostic implications and delineated three molecular NSCLC subtypes with distinct outcomes, pathological characteristics, biological pathways, and immune profiles.

Combined Effect of Nanoparticles of Silver and Silica to HeLa Cells: Synergistic Internalization and Toxicity.

The wide range of applications and the enormous production of nanomaterials have raised the possibility that humans may simultaneously contact with various nanomaterials through multiple routes. Although numerous toxicity studies have been conducted on the toxicity of nanomaterials, knowledge of the combined toxicity of nanomaterials remains limited. Herein, the combined toxic effects of the two types of the most widely used nanomaterials, silver and silica, were studied on HeLa cells.

Establishment of a rapid method for the detection of canis based on recombinase-mediated thermostable nucleic acid amplification technology.

Objective: To establish a rapid detection method for canine using recombinase-aided amplification (RAA) technology.

Methods: The outer membrane protein 25 gene fragment (Omp25) of canis was targeted. Primers and fluorescent probes were designed and synthesized, and recombinant plasmids were constructed as standards.

Shenlian Decoction Ameliorates LPS-Related Inflammation in db/db Mice: Coupling Network Pharmacology With Experimental Verification.

Shenlian (SL) decoction, a renowned traditional Chinese formula for diabetes mellitus, has also been employed to treat intestinal disorders. Previous studies have demonstrated the efficacy of SL decoction in regulating blood glucose and intestinal bacteria. Nevertheless, further analysis is required to elucidate the mechanistic link between SL decoction-mediated improvement of intestinal function and treatment of Type 2 diabetes mellitus (T2DM).