Effects of Combined Salt and Heat Stress on Agronomic Traits, Photosynthetic Parameters, and Physiological Biochemistry in Six Alfalfa ( L.) Cultivars.

Climate change due to global warming increases the susceptibility of plants to multiple combined stresses. Soil salinization and high temperature stresses that co-occur in arid/semiarid regions severely restrict the growth and development of plants. Although alfalfa ( L.

E7 peptide and magnesium oxide-functionalized coaxial fibre membranes enhance the recruitment of bone marrow mesenchymal stem cells and promote bone regeneration.

Background: The repair of bone defects remains a significant clinical challenge. Although magnesium (Mg)-based biomimetic scaffolds are widely utilized for bone defect repair, the release of Mg²⁺ ions often leads to an alkaline microenvironment, thereby adversely affecting bone regeneration. Regenerative medicine strategies that leverage the recruitment of endogenous bone marrow mesenchymal stem cells (BMSCs) offer a novel approach to treating bone defects.

[Effects of different concentrations of ropivacaine in femoral nerve block on early motor function following total knee arthroplasty].

Objective: To compare the effects of different concentrations of ropivacaine femoral nerve block on postoperative pain and early exercise fllowing total knee arthroplasty(TKA).

Methods: A total of 90 patients who underwent primary TKA between September 2022 and February 2023 were consecutively enrolled in this study. The cohort consisted of 34 males and 56 females, with a mean age of (66.

The Effects of and on the Characteristics and Microbial Community of Silage.

The utilization of nettle () as feed is restricted by its material properties (antibacterial activity and high buffering capacity). This study hypothesized that the use of lactic acid bacteria (LAB) attached to nettles can improve these problems. (LG), (PP), and LG + PP (LP) isolated from nettles were inoculated into nettle silage to explore nutrient retention and the microbial community structure.

Effect of Inoculation with and Cellulase on the Quality of Mulberry Silage.

Lactic acid bacteria (LAB) and cellulase have been used as additives to improve the fermentation quality of mulberry silage. This study investigated the dynamics of fermentation characteristics and bacterial communities during 60-day ensiling through three established treatment groups: Control (no inoculation), (LP) inoculation as well as combination of and cellulase inoculation group (LPC). The results showed that compared with the Control group, the LP and LPC treatments significantly reduced the loss of dry matter, soluble carbohydrates, and crude protein ( < 0.

Self-assembled copper-amino acid nanoleaves for targeted treatment of deep-seated bacterial infections via chemodynamic therapy and cuproptosis-like death.

Transition metal ions such as Cu are promising broad-spectrum bactericidal agents. However, transition metal ions are not used to treat deep-seated bacterial infections in clinic owing to easy deactivation by proteins and lack of targeting ability. Herein, hyaluronic acid (HA)-modified copper-N-acetyl-l-cysteine (NAC) nanoleaves (CNH NLs) are developed to treat deep-seated bacterial infections by targeted delivery of Cu to infection sites.

Fusobacterium mortiferum and its metabolite 5-aminovaleric acid promote the development of colorectal cancer in obese individuals through Wnt/β-catenin pathway by DKK2.

Colorectal cancer (CRC) is one of the most prevalent cancers worldwide, with high incidence and mortality rates. An increasing body of research suggests that obesity is a significant risk factor for the development of CRC. Moreover, recent findings have highlighted the close association between the gut microbiota and both obesity and CRC.

Involvement of 4-pentenoic acid in causing quality deterioration of nettle silage: study of antibacterial mechanism.

Unlabelled: Nettle showed several benefits for animals as an unconventional feed source, but it is difficult to ensile in order to be better utilized. Recently, nettle has been utilized to alternate alfalfa as ruminant feed, but it is usually poor in ensiling quality. To determine the endogenous component influencing ensiling, we investigated the effect of on the characteristics of nettle silage and the mechanism of action of these substances against .

Side-Chain Engineering of NIR-II-Emissive Aggregation-Induced Emission Luminogens to Boost Photodynamic and Photothermal Antimicrobial Therapy.

The development of antibiotic resistance has made multidrug-resistant bacterial and fungal infections one of the most serious health problems worldwide. Photothermal therapy (PTT) and photodynamic therapy (PDT) have received increasing attention in antimicrobial fields due to their precision treatment and less susceptibility to inducing resistance. In particular, developing second near-infrared (NIR-II, 1000-1700 nm)-emissive semiconducting polymers for phototheranostics is highly desirable but remains challenging due to the lack of rational molecular design guidelines.

Responses of the physiological characteristics and endophytic bacteria of Urtica cannabina to simulated drought stress.

Drought is widespread worldwide and has a negative impact on the growth and development of plants. As a kind of high-quality feed resource with great potential, nettle is also facing the severe test of drought stress. At present, more and more attention has been paid to the strategy of microbial drought resistance, which is expected to bring a turning point for alleviating the survival pressure of nettles under drought.

Prognostic role of STMN1 expression and neoadjuvant therapy efficacy in breast cancer.

Purpose: breast cancer is common and highly malignant, currently, STMN1 was found to be associated with several human malignancies. The purpose of this study is to investigate STMN1 expression in breast cancer and explore its role in disease progression and its interaction with neoadjuvant therapy efficacy.

Methods: we analyzed the tissue STMN1 mRNA expression in BC tissue samples from 105 patients received with neoadjuvant therapy using qPCR between 2019 and 2022.

Injectable Nanocomposite Hydrogels for Intervertebral Disc Degeneration: Combating Oxidative Stress, Mitochondrial Dysfunction, and Ferroptosis.

Intervertebral disc degeneration (IVDD) is a major cause of low back pain, where oxidative stress and mitochondrial dysfunction are key contributors. Additionally, ferroptosis, an iron-dependent form of cell death, is identified as a critical mechanism in IVDD pathogenesis. Herein, the therapeutic potential of gallic acid (GA)-derived PGA-Cu nanoparticles, enhanced with functional octapeptide (Cys-Lys-His-Gly-d-Arg-d-Tyr-Lys-Phe, SS08) to build the mitochondria-targeted nanoparticles (PGA-Cu@SS08), and embedded within a hydrogel matrix to form a nanocomposite hydrogel, is explored.

The role of cyclin dependent kinase molecules in the pathogenesis and immune cell infiltration of TNBC in silicosis: Based on core stem cell related genes TPX2 and CCNA2.

Silicosis is a pulmonary fibrotic disease caused by long-term inhalation of silica dust. CDKs regulate the process of cell cycle by binding with cyclin. This study revealed the role of cyclin-dependent kinase molecules in the pathogenesis of TNBC in silicosis and analyzed its influence on immune cell infiltration.

AKT1 Phosphorylates FDX1 to Promote Cuproptosis Resistance in Triple-Negative Breast Cancer.

Cuproptosis, a recently defined copper-dependent cell death pathway, remains largely unexplored in tumor therapies, particularly in breast cancer. This study demonstrates that triple-negative breast cancer (TNBC) bears a relatively elevated copper levels and exhibits resistance to cuproptosis. Mechanistically, copper activates the AKT signaling pathway, which inhibits ferredoxin-1 (FDX1), a key regulator of cuproptosis.

Glutamate molecular structure and protein affect the inhibition of breast cancer cell metastasis: Cell-derived exosomes inhibitory effects through the MAPK signaling pathway.

The aim of this study was to investigate the inhibitory effect of glutamate molecular structure and protein on breast cancer cell metastasis and the potential inhibitory mechanism of cell-derived exosomes via MAPK signaling pathway. Breast cancer cell lines with high metastatic potential were selected by in vitro cell culture technique. The effects of specific inhibitors of glutamic acid on the proliferation and metastasis of breast cancer cells were studied.

Rubber band ligation or shaobei injection for the endoscopic treatment of symptomatic hemorrhoids? A retrospective study.

Background: It is unknown whether Shaobei injection is superior to band ligation for endoscopic symptomatic hemorrhoid treatment. We compared the clinical efficacy, safety, and health economics of the two techniques.

Aims: This study aims to compare the efficacy, safety and health economics of endoscopic injection of Shaobei and endoscopic ligation in the treatment of grade I-IV symptomatic hemorrhoids.

Analysis of treatment methods and relapse factors of postoperative anastomotic stenosis in colorectal cancer.

Background: Anastomotic stenosis following surgical intervention for colorectal cancer is a frequently encountered complication. Nevertheless, the optimal approach to effectively manage anastomotic stenosis at varying distances from the anal margin remains uncertain. The primary objective of this research endeavor was to explore the risk factors associated with recurrent anastomotic stenosis subsequent to colorectal cancer surgery, as well as to evaluate potential strategies for its management.

Synergistic wall digestion and cuproptosis against fungal infections using lywallzyme-induced self-assembly of metal-phenolic nanoflowers.

Fungi are very common infectious pathogens, which may cause invasive and potentially life-threatening infections. However, the efficacy of antifungal medications remains limited. Herein, a Cu-phenolic nanoflower is designed to combat fungal infections by combining cuproptosis and cell wall digestion.

Nanomaterial-based therapeutics for enhanced antifungal therapy.

The application of nanotechnology in antifungal therapy is gaining increasing attention. Current antifungal drugs have significant limitations, such as severe side effects, low bioavailability, and the rapid development of resistance. Nanotechnology offers an innovative solution to address these issues.

Direct visualization endoscopic retrograde appendicitis therapy for treatment of acute uncomplicated appendicitis.

Objective: To investigate the diagnostic and therapeutic value of direct visualization (single-use eyeMax subscope) endoscopic management of acute uncomplicated appendicitis.

Methods: Thirty-six patients diagnosed with acute uncomplicated appendicitis, confirmed by computed tomography or ultrasonography, from Jan 2023 to Feb 2024 were enrolled in this study. We collected demographics, colonoscopy findings, subscope findings, clinical outcomes of endoscopic retrograde appendicitis therapy (ERAT), and adverse events associated with ERAT.

An elastomer with in situ generated pure zwitterionic surfaces for fibrosis-resistant implants.

Polymeric elastomers are widely utilized in implantable biomedical devices. Nevertheless, the implantation of these elastomers can provoke a robust foreign body response (FBR), leading to the rejection of foreign implants and consequently reducing their effectiveness in vivo. Building effective anti-FBR coatings on those implants remains challenging.

Evaluation of the efficacy of autogenous tibial periosteal bone grafting in the treatment of osteochondral lesions of the talus and analysis of three-dimensional factors in the necrotic zone.

Purpose: This study aims to explore the clinical value of autogenous tibial periosteal bone grafting in the treatment of osteochondral lesions of the talus (OLT) and analyze the three-dimensional factors in the necrotic zone of the talus.

Methods: A retrospective analysis was performed on 36 patients who underwent autogenous tibial periosteal bone grafting in the Foot and Ankle Surgery Department of our hospital between September 2018 and September 2022. The American Orthopaedic Foot and Ankle Society (AOFAS), Visual Analogue Scale (VAS), and Chinese Short-Form 36 Health Survey (SF-36) were used to evaluate treatment efficacy prior to surgery and at the last follow-up.