Mapping the global research landscape and trends of older people living alone: a bibliometric analysis.

Background: The global aging trend is becoming increasingly severe, leading to a rise in the number of older adults living alone. As research on this population grows, a comprehensive analysis is essential.

Objective: This study examines the current state of research on older adults living alone, identifies key trends and emerging topics, and provides a foundation for future investigations.

Utilization of rehabilitation services among older adults with physical disabilities: the interactive influence of regional development and socioeconomic position.

Background: The utilization of rehabilitation services by older adults with disabilities is an important health task in the context of population aging and disability expansion. This study aims to explore whether there are structural differences in the relationship between the socioeconomic position (SEP) of older adults with physical disabilities (PD) and their utilization of rehabilitation services across urban and rural areas.

Methods: A total of 19,782 observations of older adults with PD from the 2007-2013 China National Survey on Disability and the 2006 Second National Sample Survey on Disability were included in this study.

Acupuncture as an Adjunctive Therapy to Etoricoxib for Patients with Acute Gouty Arthritis: Protocol for a Multicenter Randomized Controlled Trial.

Purpose: Acute gouty arthritis (AGA) is often managed with non-steroidal anti-inflammatory drugs. However, patients still tolerate severe pain before the analgesic takes effect. Early adjuvant analgesia is necessary in the pharmacological management of AGA.

Temporal Effects of Disease Signature Genes and Core Mechanisms in the Hyperacute Phase of Acute Ischemic Stroke: A Bioinformatics Analysis and Experimental Validation.

The pathophysiological progression during the hyperacute phase of acute ischemic stroke (AIS) critically determines clinical outcomes. Identification of phase-specific biomarkers and elucidation of their temporal regulatory mechanisms are pivotal for optimizing therapeutic interventions. Disease signature genes and their mechanisms of action were screened based on the Gene Expression Omnibus database.

A selenium nanozyme: Light/mercury dual-enhanced oxidase mimicry for simultaneous ultra-sensitive detection and efficient removal of mercury ions, and superior photocatalytic bacterial disinfection.

Wastewater from aquatic product processing, often laden with heavy metals like Hg²⁺ and organic waste that promotes harmful bacterial proliferation, poses substantial environmental and public health risks if left untreated. This study introduces a novel gallic acid-modified chitosan stabilized selenium nanoparticle (GA-CS@SeNPs) capable of simultaneously detecting and removing Hg²⁺ while eradicating harmful bacteria. GA-CS@SeNPs exhibit light-stimulated and light/Hg²⁺ dual-enhanced oxidase-like activity, with a remarkable adsorption capacity of 1895.

Anti-RPL30 as a novel biomarker for enhanced diagnosis of autoantibody-negative primary biliary cholangitis.

Background: The diagnosis of primary biliary cholangitis (PBC) remains challenging, particularly in cases where anti-mitochondrial antibody (AMA), anti-mitochondrial E2 subunit antibody (AMA-M2), anti-glycoprotein 210 (anti-gp210), and anti-speckled protein 100 (anti-Sp100) are all negative. In such instances, the condition is often confirmed through a liver needle biopsy.

Aim: To identify additional plasma biomarkers for non-invasive diagnostic methods of PBC.

The role of myeloperoxidase in the pathogenesis of stroke.

Stroke is the leading cause of mortality and morbidity worldwide, significantly impacting human welfare and overall health. Myeloperoxidase (MPO), a heme peroxidase secreted by neutrophils, plays a crucial role in the body's defense mechanisms, exhibiting pro-inflammatory and pro-oxidative properties. Additionally, MPO compromises the structural integrity and functional capacity of blood vessels, potentially leading to the formation and dislodgement of atherosclerotic plaques, vascular stenosis, thrombosis, and ultimately contributing to stroke occurrence.

Monitoring the Growth and Habitat Shifts of Epiphyllous Liverworts in Subtropical Forests of China.

Understanding the extent to which species can adjust their ranges in response to climate change and track areas of suitable climatic conditions is vital for conservation efforts. Nonetheless, the observed changes in species distribution may also result from inadequate field data. This is particularly relevant for epiphyllous liverworts, which exhibit a poikilohydric lifestyle that makes them more vulnerable to climatic fluctuations than many other higher plants.

Thermal sensitization of acupoints in patients with knee osteoarthritis: A cross-sectional case-control study.

Objective: Varied acupoint selections represent a potential cause of the uncertainty surrounding the efficacy of acupuncture for knee osteoarthritis (OA). Skin temperature, a guiding factor for acupoint selection, may help to address this issue. This study explored thermal sensitization of acupoints used for the treatment of knee OA.

Hypoxia-induced PRMT1 methylates HIF2β to promote breast tumorigenesis via enhancing glycolytic gene transcription.

Hypoxia-induced metabolic reprogramming is closely linked to breast cancer progression. Through transcriptomic analysis, we identified PRMT1 as a direct target of hypoxia-inducible factor 1α (HIF1α) under hypoxic conditions in breast cancer cells. In turn, PRMT1 enhances the expression of HIF1α-driven glycolytic genes.

Temporal trends of avoidable mortality among children under-five in China from 2004 to 2021.

Background: Avoidable mortality (AM) refers to deaths preventable through effective prevention measures or timely medical treatment, and reducing AM is crucial for improving child survival rates. We conducted an analysis of the situation and temporal trends of AM among under-five (U5) children in China.

Methods: Data were extracted from the China Death Surveillance Dataset.

WCSGNet: a graph neural network approach using weighted cell-specific networks for cell-type annotation in scRNA-seq.

Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for understanding cellular heterogeneity, providing unprecedented resolution in molecular regulation analysis. Existing supervised learning approaches for cell type annotation primarily utilize gene expression profiles from scRNA-seq data. Although some methods incorporated gene interaction network information, they fail to use cell-specific gene association networks.

Danggui-Shaoyao-San Can Ameliorate Alzheimer's Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology.

Background: Danggui-Shaoyao-San (DSS) is a traditional Chinese medicine prescription with a history of nearly 2000 years, originally widely used for gynecological diseases, and in recent years research has found that DSS also has a good therapeutic effect on Alzheimer's disease (AD).

Purpose: The objective is to investigate the metabolic components of the DSS in the blood and the potential mechanisms for AD.

Materials And Methods: Liquid chromatography‒mass spectrometry (LC-MS) combined with gas chromatography‒mass spectrometry (GC-MS) based non-targeted metabolomics were used to conduct in-depth research.

Antibacterial and Cytotoxic Methylthioether-Containing Cytochalasins from AS-506, an Endozoic Fungus Associated with Deep-Sea Sponge of Magellan Seamounts.

Ten cytochalasin derivatives, including six new methylthioether-containing chaetoglobosins (thiochaetoglobosins A-F, ), a new related congener (18-nor-prochaetoglobosin II, ), and three known unsulfured counterparts (), were isolated and identified from AS-506, an endozoic fungus isolated from a deep-sea sponge, which was collected from Magellan Seamounts in the Western Pacific Ocean. Their structures were determined by extensive interpretation of the spectroscopic and X-ray crystallographic data, as well as by ECD calculations. Structurally, thiochaetoglobosins A-F () represent the first examples of chaetoglobosin derivatives containing a methylthioether group in the molecules, while 18-nor-prochaetoglobosin II () is the first 18-nor-chaetoglobosin derivative.

Personalized Vascularized Tumor Organoid-on-a-Chip for Tumor Metastasis and Therapeutic Targeting Assessment.

While tumor organoids have revolutionized cancer research by recapitulating the cellular architecture and behaviors of real tumors in vitro, their lack of functional vasculature hinders their attainment of full physiological capabilities. Current efforts to vascularize organoids are struggling to achieve well-defined vascular networks, mimicking the intricate hierarchy observed in vivo, which restricts the physiological relevance particularly for studying tumor progression and response to therapies targeting the tumor vasculature. An innovative vascularized patient-derived tumor organoids (PDTOs)-on-a-chip with hierarchical, tumor-specific microvasculature is presented, providing a versatile platform to explore tumor-vascular dynamics and antivascular drug efficacy.

 (Euphorbiaceae), a new species from Southwest China based on morphological characters and phylogenetic evidence.

M.T.An & J.

Overall Reporting Quality of Randomized Controlled Trials of Acupuncture for Knee Osteoarthritis: A Systematic Review.

Objective: To evaluate the reporting quality of randomized controlled trials (RCT) of acupuncture for knee osteoarthritis and explore factors associated with the reporting.

Study Design And Setting: Eight databases were searched from inception to August 2024 to assess the quality of acupuncture for knee osteoarthritis RCTs based on the CONSORT, the STRICTA, and the CONSORT-Outcomes. We performed regression analyses on pre-specified study characteristics to explore factors associated with reporting quality.

Longitudinal assessment of the progression of severe keratoconus based on corneal topography.

We aimed to assess long-term disease progression in patients with severe keratoconus (KC). Clinical records of 125 patients (201 eyes) with severe KC followed-up for > 12 months were retrospectively analyzed. From these, 28 patients (31 eyes) were included.

In-Depth Examination of the Functionality and Performance of the Internet Hospital Information Platform: Development and Usability Study.

Background: Internet hospitals (IHs) have rapidly developed as a promising strategy to address supply-demand imbalances in China's medical industry, with their capabilities directly dependent on information platform functionality. Furthermore, a novel theory of "Trinity" smart hospital has provided advanced guidelines on IH constructions.

Objective: This study aimed to explore the construction experience, construction models, and development prospects based on operational data from IHs.

Boosting bioelectricity generation in bioelectrochemical systems with nitrogen-doped three-dimensional graphene aerogel anode.

Bioelectricity generation by electrochemically active bacteria has become particularly appealing due to its vast potential in energy production, pollution treatment, and biosynthesis. However, developing high-performance anodes for bioelectricity generation remains a significant challenge. In this study, a highly efficient three-dimensional nitrogen-doped macroporous graphene aerogel anode with a nitrogen content of approximately 4.

Safety of low-intensity extracorporeal shock wave therapy in prostate disorders: in vitro and in vivo evidence.

Recent evidence suggests that low-intensity extracorporeal shock wave therapy (Li-ESWT) is a promising treatment for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS); however, its safety in pelvic organs, particularly prostate tissues and cells, remains unclear. The current study evaluates the risks of prostate cell damage or oncogenesis following the administration of Li-ESWT for prostatitis. To this end, a robust in vitro model (Cell Counting Kit-8 [CCK-8] assay, clone formation assay, cell scratch assay, lactate dehydrogenase [LDH] release assay, flow cytometry, and immunoblotting assay) was designed to examine the effects of Li-ESWT on cell proliferation, clonogenicity, migration, membrane integrity, and DNA damage.

Dynamics of endothelial cells migration in nature-mimicking blood vessels.

Endothelial cells (ECs) migration is a crucial early step in vascular repair and tissue neovascularization. While extensive research has elucidated the biochemical drivers of endothelial motility, the impact of biophysical cues, including vessel geometry and topography, remains unclear. Herein, we present a novel approach to reconstruct 3D self-assembly blood vessels-on-a-chip that accurately replicates real vessel geometry and topography, surpassing conventional 2D flat tube formation models.

Factors associated with out-of-school physical activity among Chinese children and adolescents: A stratified cross-sectional study.

Objective: This observational study examined the factors associated with the physical activity (PA) of children and adolescents outside of school within the framework of Problem Behavior Theory (PBT).

Methods: This cross-sectional study obtained data from 6528 children and adolescents aged 6-16 years recruited from ten schools in Shanghai, China. The questionnaire measured out-of-school PA and PBT-based correlates.