Chromosome-level genome assembly of the Phoxinus lagowskii.

Phoxinus lagowskii is an economically important freshwater fish species native to China. In this study, a chromosome-level genome assembly of P. lagowskii was assembled using PacBio and Hi-C sequencing technologies.

Environmental levels of bis(2-ethylhexyl)-tetrabromophthalate (TBPH) cause thyroid toxicity and early carcinogenic signals in juvenile fish.

Exposure to novel brominated flame retardants (NBFRs) may engender thyroid dysfunction and even increase thyroid cancer risk. However, information regarding their thyroid toxic or disorder potential is primarily limited to thyroid hormone homeostasis. Here, we conducted a 28-day exposure experiment with juvenile rare minnows (Gobiocypris rarus) to 0.

Molecular characterization and expression analysis of IL-21 and its four receptor genes in yellow catfish (Pelteobagrus fulvidraco) in response to Edwardsiella ictaluri infection.

Interleukin 21 (IL-21) is a member of the IL-2 family and a pleiotropic cytokine that exerts its effects by binding to its receptor complex (IL-21Rα and γ chain). Due to the critical role and multifunctionality of IL-21, its functions have been extensively studied in mammals; however, there has been limited research on IL-21 in teleosts. In this study, the cDNA sequences of Pf_IL-21, Pf_IL-21Rα.

Pollution characteristics and health risk assessment of organophosphate esters in agricultural products from different regions of China.

Organophosphate esters (OPEs) have been extensively utilized as flame retardants and plasticizers, raising significant concerns because of their persistence in the environment, mobility, and potential health risks. This study systematically analyzed the occurrence, spatial distribution, and health risks of 21 target OPEs in 232 agricultural product samples (including vegetables, fruits, grains, and animal products) collected from five regions in China: Shanghai, Liaoning, Qinghai, Sichuan, and Tibet. The results showed that the ∑ OPEs concentrations ranged from ND (not detected) to 2381.

Molecular characterization, expression analysis and function identification of Pf_IL-12p35, Pf_IL-23p19 and Pf_IL-12p40 genes in yellow catfish (Pelteobagrus fulvidraco).

The interleukin-12 (IL-12) family is a class of heterodimeric cytokines that play crucial roles in pro-inflammatory and pro-stimulatory responses. Although some IL-12 and IL-23 paralogues have been found in fish, their functional activity in fish remains poorly understood. In this study, Pf_IL-12p35a/b, Pf_IL-23p19 and Pf_IL-12p40a/b/c genes were cloned from yellow catfish (Pelteobagrus fulvidraco), four α-helices were found in Pf_IL-12p35a/b and Pf_IL-23p19.

Thermocline stratification favors phytoplankton spatial overlap and species diversity in a subtropical deep reservoir.

Spatial segregation of species along opposing resource gradients is a major research topic in ecology as it enables the coexistence and maintenance of high diversity. Thermocline stratification causes heterogeneous resource distribution, however, the effect of thermocline stratification on phytoplankton dynamic in-depth profiles is still unclear. To understand the underlying mechanism behind the effects of thermocline stratification on phytoplankton species diversity in stratified lakes, the monthly changes in thermocline parameters, deep chlorophyll maxima (DCM) parameters, spatial overlap (SO) among major phytoplankton taxonomic groups, and phytoplankton species diversity were evaluated in Lake Qiandaohu in the Zhejiang Province of China from April 2017 to December 2018.

Predictable shifts in diversity and ecosystem function in phytoplankton and zooplankton communities along thermocline stratification intensity continua.

Thermocline stratification is a global threat to water quality in drinking water reservoirs, though its underlying mechanisms are not clear. The impacts of thermocline stratification intensity on biodiversity-ecosystem relationships were assessed using phytoplankton and zooplankton indicators from four stages of stratification in Lake Qiandaohu. There were significant differences in biomass, species diversity, and resource use efficiency (RUEpp = phytoplankton biomass/total phosphorus) for phytoplankton between continuous stratification and mixing periods, but only in FDis and RaoQ diversity indices for zooplankton.

Probiotic Lactobacillus rhamnosus alleviates the neurotoxicity of microcystin-LR in zebrafish (Danio rerio) through the gut-brain axis.

Microcystin-LR (MCLR) is one of the most toxic cyanobacterial toxins and is harmful to the central nervous system of fish. Probiotic additives can improve neuroendocrine function in fish. Although both MCLR and probiotics aim at the nervous system, whether they interact with each other and the mechanisms remain unexplored.

Do submerged macrophyte species influence crustacean zooplankton functional group richness and their resource use efficiency in the low-light environment?

During the high grazing of epiphytic zooplankton in submerged macrophyte beds, the changes in crustacean zooplankton functional groups are crucial for stabilizing a clear water state in shallow lakes. However, submerged macrophytes often experience low-light stress due to many ecological processes. It is unclear whether submerged macrophytes alter the zooplankton functional group and their resource use efficiency in the low-light environment.

Tris(1,3-dichloro-2-propyl) phosphate causes female-biased growth inhibition in zebrafish: Linked with gut microbiota dysbiosis.

Tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) is ubiquitous in aquatic environment, but its effect on intestinal health of fish has yet not been investigated. In the present study, the AB strain zebrafish embryos were exposed to environmentally realistic concentrations (0, 30, 300, and 3000 ng·L) of TDCIPP for 90 days, after which the fish growth and physiological activities were evaluated, and the intestinal microbes were analyzed by 16S rRNA gene high-throughput sequencing. Our results manifested that the body length and body weight were significantly reduced in the female zebrafish but not in males.

Molecular Characterization of Nine TRAF Genes in Yellow Catfish () and Their Expression Profiling in Response to Infection.

The yellow catfish () is an economic fish with a large breeding scale, and diseases have led to huge economic losses. Tumor necrosis factor receptor-associated factors (TRAFs) are a class of intracellular signal transduction proteins that play an important role in innate and adaptive immune responses by mediating NF-κB, JNK and MAPK signaling pathways. However, there are few studies on the TRAF gene family in yellow catfish.

Molecular and functional characterization of teleost-specific Interleukin-17N in yellow catfish (Pelteobagrus fulvidraco).

In mammals, six interleukin-17 (IL-17) genes, as potent pro-inflammatory cytokines, all accelerate the inflammatory responses. In teleosts, seven IL-17 genes have been found in various species, but little is known about the function of teleost-specific IL-17N. In this study, teleost IL-17N and IL-17A/F2 genes all had six conserved cysteine residues forming three intrachain disulfide bridges, the length of three exons of teleost IL-17N gene was similar to that of teleost IL-17A/F2 gene, and the neighbor-joining (NJ) phylogenetic tree showed that teleost IL-17N was clustered with vertebrate IL-17A/F, implying that teleost IL-17N gene may be a paralog of teleost IL-17A/F gene.

Dualistic effects of bisphenol A on growth, photosynthetic and oxidative stress of duckweed (Lemna minor).

In this study, we exposed duckweed (Lemna minor), a floating freshwater plant, to BPA at different concentrations (0, 1, 5, 20, and 50 mg/L) for 7 days so as to investigate the effects of BPA on its growth, photosynthesis, antioxidant system, and osmotic substances. It was found that BPA had the acute toxic effects of "low promotion and high inhibition" on growth and photosynthesis. Specifically, BPA at a low concentration (5 mg/L) significantly promoted the plant growth and improved the concentration of photosynthetic pigments (chlorophyll a, b, and total Chl ) of L.

Expression and Function Analysis of Interleukin-17A/F1, 2, and 3 Genes in Yellow Catfish (): Distinct Bioactivity of Recombinant IL-17A/F1, 2, and 3.

In mammals, Interleukin-17 cytokine family plays critical roles in both acute and chronic inflammatory responses. In fish species, three Interleukin-17A/F (IL-17A/F) genes have been identified to be homologous to mammalian IL-17A and IL-17F, but little is known about their functional activity. In this study, _IL-17A/F1, 2 and 3 genes were cloned from yellow catfish () and they differed in protein structure and exon length, implying that they may have divergent bioactivity.

Organic carbon quantity and quality jointly triggered the switch between dissimilatory nitrate reduction to ammonium (DNRA) and denitrification in biofilters.

The effect of organic carbon (OC) quality and quantity on switch between dissimilatory nitrate reduction to ammonium (DNRA) and denitrification (DEN) was studied in biofilter systems. High OC in matrix could promote significantly nitrate (NO-N) removal due to the reinforce of DEN. Sodium acetate (SA) addition in influent further fueled NO-N removal in groups with low OC in matrix but increased ammonium (NH-N) and nitrite (NO-N) accumulation in groups with high OC in matrix.

Molecular characterization, expression analysis and function identification of Pf_TNF-α and its two receptors Pf_TNFR1 and Pf_TNFR2 in yellow catfish (Pelteobagrus fulvidraco).

Inflammation is a common manifestation of body immunity and mediates a cascade of cytokines. Tumor necrosis factor-α (TNF-α), as a multi-effect cytokine, plays an important role in the inflammatory response by interacting with its receptor (TNFR). In this study, Pf_TNF-α, Pf_TNFR1 and Pf_TNFR2 genes were cloned from yellow catfish (Pelteobagrus fulvidraco), and bioinformatics analyses showed that the three genes were conserved and possessed similar sequence characteristics as those of other vertebrates.

The efficacy of DNA barcoding in the classification, genetic differentiation, and biodiversity assessment of benthic macroinvertebrates.

Macroinvertebrates have been recognized as key ecological indicators of aquatic environment and are the most commonly used approaches for water quality assessment. However, species identification of macroinvertebrates (especially of aquatic insects) proves to be very difficult due to the lack of taxonomic expertise in some regions and can become time-consuming. In this study, we evaluated the feasibility of DNA barcoding for the classification of benthic macroinvertebrates and investigated the genetic differentiation in seven orders (Insecta: Ephemeroptera, Plecoptera, Trichoptera, Diptera, Hemiptera, Coleoptera, and Odonata) from four large transboundary rivers of northwest China and further explored its potential application to biodiversity assessment.

The Dynamic Immune Response of Yellow Catfish () Infected With Presenting the Inflammation Process.

is a highly destructive pathogen in cultured yellow catfish, thus it was very necessary to study the immune response of yellow catfish against bacterial infection. In this study, RNA-Seq technology was used to study the immune response in two distinct tissues of yellow catfish at eight different time points (h) after infection. The number of differentially expressed genes (DEGs) in the spleen and liver was low at 3 h and 6 h post-infection, respectively.

Interactions between commensal bacteria and viral infection: insights for viral disease control in farmed animals.

Viral diseases cause serious economic loss in farmed animals industry. However, the efficacy of remedies for viral infection in farmed animals is limited, and treatment strategies are generally lacking for aquatic animals. Interactions of commensal microbiota and viral infection have been studied in recent years, demonstrating a third player in the interaction between hosts and viruses.

Exposure to environmentally relevant concentrations of deltamethrin renders the Chinese rare minnow (Gobiocypris rarus) vulnerable to Pseudomonas fluorescens infection.

In this study, to assess the immunotoxicity of deltamethrin on fish, adult Chinese rare minnows (Gobiocypris rarus) were exposed to 0.1, 0.3, and 1 μg/L deltamethrin for 28 d.

Global diversity and genetic landscape of natural populations and hatchery stocks of largemouth bass micropterus salmoides across American and Asian regions.

Although largemouth bass Micropterus salmoides has shown its extremely economic, ecological, and aquacultural significances throughout the North American and Asian continents, systematic evaluation of genetic variation and structure of wild and cultured populations of the species is yet to be documented. In this study, we investigated the genetic structure of M. salmoides from 20 wild populations and five cultured stocks across the United States and China using eight microsatellite loci, which are standard genetic markers for population genetic analysis.

Comprehensive analysis of population genetics of in the Irtysh River: Abiotic and biotic factors.

As a widely distributed species along the Irtysh River, (Kaschtschenko, 1899) was used as a model to investigate genetic diversity and population structure as well as the influence of environmental factors on population genetics. In this study, we specifically developed 12 polymorphic microsatellite loci. The analysis of microsatellite and mtDNA markers revealed a high and a moderate genetic diversity across seven populations, respectively.

The immune responses of the Chinese rare minnow (Gobiocypris rarus) exposed to environmentally relevant concentrations of cypermethrin and subsequently infected by the bacteria Pseudomonas fluorescens.

In the present study, to assess the immunotoxicity of cypermethrin (CYP) in fish, Chinese rare minnows (Gobiocypris rarus) were exposed to environmentally relevant concentrations (0.15, 0.5, and 1.

Comparative transcriptome analysis reveals potential evolutionary differences in adaptation of temperature and body shape among four Percidae species.

Considering the divergent temperature habitats and morphological traits of four Percidae species: yellow perch (Perca flavescens), Eurasian perch (Perca fluviatilis), pike perch (Sander lucioperca), and ruffe (Gymnocephalus cernua), we stepped into the transcriptome level to discover genes and mechanisms that drive adaptation to different temperature environments and evolution in body shape. Based on 93,566 to 181,246 annotated unigenes of the four species, we identified 1,117 one-to-one orthologous genes and subsequently constructed the phylogenetic trees that are consistent with previous studies. Together with the tree, the ratios of nonsynonymous to synonymous substitutions presented decreased evolutionary rates from the D.