2',4'-dihydroxychalcone induces ferroptosis through ERO1A/GPX4 regulatory axis in cholangiocarcinoma.

Background: Cholangiocarcinoma (CCA) , an aggressive cancer often detected late, carries a grim prognosis. 2',4'-Dihydroxychalcone (2',4'-DHC), a flavonoid monomer isolated from Empetrum nigrum, has demonstrated notable anti-tumor activity in multiple cancer types. However, its therapeutic mechanism in cholangiocarcinoma remains poorly understood, especially regarding the regulation of ferroptosis, a mechanism that has not yet been fully elucidated in this disease.

Bidirectional Mendelian randomization analysis of the causal relationship between inflammatory bowel disease and Hashimoto thyroiditis.

This study aimed to explore the bidirectional causal relationship between inflammatory bowel disease (IBD) and Hashimoto thyroiditis (HT) using Mendelian randomization (MR) analysis. While both conditions are characterized by persistent inflammation and immune dysregulation, the direction of causality remains unclear. We performed a 2-sample bidirectional MR analysis using summary statistics from genome-wide association studies for IBD and HT.

Study on the Effect of Antibiotics on the Efficacy of PD-1 Inhibitors and Its Regulatory Mechanism via the Intestinal Bacterial Community.

This study investigated the impact of antibiotics on the therapeutic efficacy of PD-1 inhibitors and their association with the intestinal microbiota. Using a Hepa1-6 hepatocellular carcinoma mouse model, various antibiotics (vancomycin, colistin, and combination antibiotics) were administered alongside a PD-1 inhibitor. The results indicated that antibiotic treatment significantly altered the intestinal microbiota composition, with vancomycin and the combination antibiotics notably reducing PD-1 inhibitor efficacy.

Study on the Promotion of Gas Hydrate Generation by Three Different Electric Field Waveform Signals in Synergy with Surfactants.

The rapid development of the natural gas hydrate industry has put forward higher requirements for hydrate promotion technology. The exploration of methods that can simultaneously enhance both the hydrate formation rate and the final gas and water conversion efficiency has become a critical research focus. This study systematically investigated the synergistic effects of electric field (EF) signals, including three distinct waveforms (square, sine, ramp wave) at six different voltage levels, combined with four concentration gradients of the cationic surfactant called hexadecyl trimethylammonium bromide (CTAB) on the CH hydrate formation process.

Impaired ARID1A expression attenuated the immune response in gastric cancer via histone acetylation.

Background: The primary objective of this study was to examine whether ARID1A mutations confer a fitness advantage to gastric cancer from an immunological perspective, along with elucidating the underlying mechanism. Additionally, we aimed to identify the clinical potential of combining epigenetic inhibitors with immune checkpoint inhibitors to improve the efficacy of immunotherapy for gastric cancer.

Methods: The correlation between ARID1A gene expression and gastric cancer patient survival was analyzed using the GEO dataset GSE62254.

Assessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems.

In this experiment, we employed Real-time PCR(RT-PCR) and metagenomic Next-Generation Sequencing (mNGS) techniques to detect the presence of Norovirus, Rotavirus Group A, Adenovirus Group F, and Astrovirus in untreated sewage from three major hospitals. A comparison with clinical lab test outcomes revealed Norovirus as having the highest infection rate, followed by Adenovirus Group F and Rotavirus Group A. Despite not testing for Astrovirus in clinical labs, its sewage detection rate was surpassed only by Norovirus, suggesting a potentially high clinical infection rate.

Cordycepin reprogramming lipid metabolism to block metastasis and EMT via ERO1A/mTOR/SREBP1 axis in cholangiocarcinoma.

Cholangiocarcinoma (CCA) with a high malignancy is usually diagnosed as advanced and is prone to metastasis and leads to a poor prognosis. It is reported that cordycepin has anti-tumor effect. However, the molecular targets and mechanisms of cordycepin in inhibiting CCA metastasis remains unclear.

Lipid metabolism reprogramming in colorectal cancer.

The hallmark feature of metabolic reprogramming is now considered to be widespread in many malignancies, including colorectal cancer (CRC). Of the gastrointestinal tumors, CRC is one of the most common with a high metastasis rate and long insidious period. The incidence and mortality of CRC has increased in recent years.

Inhibition of TGFβ1/Smad pathway by NF-κB induces inflammation leading to poor wound healing in high glucose.

This study mainly analyzed the relationship between nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor-β (TGFβ1)/Smad under high glucose environment and its influence on wound healing. Fibroblast NIH-3T3 was used to analyze the effect of high concentration glucose (20 nmol/mL) on cell viability, migration ability, inflammation level and NF-κB pathway. Pyrrolidinedithiocarbamate (PDTC) was used to inhibit NF-κB for rescue experiments.

Comparison of the Effects of Open Surgery and Minimally Invasive Surgery on the Achilles Tendon Rupture Healing Based on Angiogenesis.

Objective: To compare the effect of three different surgical methods on rabbit Achilles tendon rupture.

Methods: The Achilles tendon transection model was constructed by cutting off the inner half of the Achilles tendon. Rabbits were divided into 4 groups: model group, open surgery (OS) group, minimally invasive surgery (MS) group, and conservative treatment (CT) group.

MiR-192-5p Alleviated Fibrosis and Inflammatory Responses of Tendon Cells by Targeting NFAT5.

Objective: To explore the effect of microRNA (miR)-192-5p on the inflammatory and fibrotic responses of tendon cells.

Methods: Tendon cells were treated with transforming growth factor-1 (TGF-1). The expression of miR-192-5p and nuclear factor of activated T cells 5 (NFAT5) in tendon cells were detected by RT-qPCR.

[Cordycepin inhibits the proliferation and migration of human gastric cancer cells by suppressing lipid metabolism via AMPK and MAPK activation].

Objective To explore the inhibitory effect of cordycepin on the proliferation and migration of gastric cancer cells and its molecular mechanism. Methods MGC-803 cells were treated with 0, 25, 50, 100 μmol/L of cordycepin and HGC-27 cells with 0, 5, 25, 50 μmol/L of cordycepin for 48 hours. The proliferation ability of MGC-803 and HGC-27 cells was detected by MTT assay and EdU assay; the colony formation ability of cells was detected by colony formation assay; both wound healing assay and cell migration assay were applied to detect the cell migration ability of MGC-803 and HGC-27 cells; the chromatin agglutination was detected by Hoechst 33342 staining; the apoptosis of gastric cancer cells was detected by annexin V-FITC/PI double labeling combined with flow cytometry; Western blot was used to measure the protein expression levels of lipid metabolism-related proteins including sterol regulatory element binding transcription factor 1 (SREBF1), fatty acid synthase (FASN), and acetyl coA carboxylase 1 (ACC1), epithelial-mesenchymal transition (EMT)-related proteins including E-cadherin, vimentin, Snail, Slug, matrix metalloproteinase 2 (MMP2), MMP9, AMPK, and phosphorylated AMPK (p-AMPK), MAPK signaling pathway-related proteins including JNK, phosphorylated JNK (p-JNK), p38 MAPK, and p-p38 MAPK, and apoptosis-related proteins including cleaved caspase-9 (c-caspase-9), c-caspase-3, and cleaved poly (ADP-ribose) polymerase (c-PARP).

Crowberry inhibits cell proliferation and migration through a molecular mechanism that includes inhibition of DEK and Akt signaling in cholangiocarcinoma.

Background: Cholangiocarcinoma (CCA) is a rare biliary adenocarcinoma related to poor clinical prognosis. Crowberry is an herbal medicine used to control inflammatory diseases and reestablish antioxidant enzyme activity. Although crowberry shows significant therapeutic efficacy in various tumors and diseases, its anticancer effects and specific molecular mechanisms in CCA are poorly understood.

Molecular insight into carbon dioxide hydrate formation from saline solution.

Carbon dioxide hydrate has been intensively investigated in recent years because of its potential use as gas and heat storage materials. To understand the hydrate formation mechanisms, the crystallization of CO hydrate from NaCl solutions was simulated at a molecular level. The influence of temperature, pressure, salt concentration and CO concentration on CO hydrate formation was evaluated.

An innovative experimental apparatus for the analysis of sand production during natural gas hydrate exploitation.

Natural gas hydrate (NGH) will become a significant potential energy source in the post-oil era due to its large reserves, wide distribution, high energy density, and low pollution. Sand production is one of the main problems that cause the impossible long-term production of NGH. This paper presents an experimental apparatus that was developed to synthesize NGH and hydrate-bearing sediments and was applied to analyze the sand production and sand control mechanism during hydrate exploitation.

Dexamethasone promotes the endoplasmic reticulum stress response of bone marrow mesenchymal stem cells by activating the PERK-Nrf2 signaling pathway.

The pathogenesis of steroid-induced avascular necrosis of femoral head (SANFH) is complex, and there is a lack of effective early prevention method. The aim of the present study was to evaluate the effect of dexamethasone (DEX) on the biological behavior of bone marrow mesenchymal stem cells (BMSCs) and to explore the possibility of DEX in the clinical treatment of SANFH. The effect of DEX on the proliferation of BMSCs was evaluated by Counting Kit-8 assay, western blot assay, and enzyme-linked immunosorbent assay.

Recombinant enterotoxin-1 (rBFT-1) promotes proliferation of colorectal cancer via CCL3-related molecular pathways.

Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide and stands among the leading causes of cancer-related deaths. Although deregulation of the microbiota in the gastrointestinal tract has been frequently described in CRC, very little is known about the precise molecular mechanisms by which bacteria and their toxins modulate the process of tumorigenesis and behavior of cancer cells. In this study, we produced recombinant enterotoxin-1 (rBFT1) and demonstrate that rBFT1 could promote cell proliferation in colorectal cancer cells and accelerate tumor growth .

Multiscale analysis of the hydrate based carbon capture from gas mixtures containing carbon dioxide.

To reveal the kinetic performance of gas molecules in hydrate growth, hydrate formation from pure CO, flue gas, and biogas was measured using in-situ Raman and macroscopic methods at 271.6 K. In the in-situ Raman measurements, Raman peaks of gases in the hydrate phase were characterised and normalised by taking the water bands from 2800 to 3800 cm as a reference, whose line shapes were not found to have a noticeable change in the conversion from Ih ice to sI hydrate.

Formation of a Low-Density Liquid Phase during the Dissociation of Gas Hydrates in Confined Environments.

The large amounts of natural gas in a dense solid phase stored in the confined environment of porous materials have become a new, potential method for storing and transporting natural gas. However, there is no experimental evidence to accurately determine the phase state of water during nanoscale gas hydrate dissociation. The results on the dissociation behavior of methane hydrates confined in a nanosilica gel and the contained water phase state during hydrate dissociation at temperatures below the ice point and under atmospheric pressure are presented.

Upregulation of TIGIT and PD-1 in Colorectal Cancer with Mismatch-repair Deficiency.

: The role of T cell Ig and ITIM domain (TIGIT) and programmed cell death-1 (PD-1) in colorectal cancer (CRC) with mismatch repair deficiency is unknown.: This was a study of 60 CRC patients with mismatch repair deficiency and 30 healthy controls between June 2015 and October 2015.: The expression of Foxp3, PD-1, and TIGIT was higher in cancer tissues compared with adjacent mucosa (all < .

Molecular Dynamics Simulation Study on the Growth of Structure II Nitrogen Hydrate.

Crystal growth of N hydrate in a three-phase system consisting of N hydrate, liquid water, and gaseous N was performed by molecular dynamics simulation at 260 K. Pressure influence on hydrate growth was evaluated. The kinetic properties including the growth rates and cage occupancies of the newly formed hydrate and the diffusion coefficient and concentration of N molecules in liquid phase were measured.

Microscopic measurements on the decomposition behaviour of methane hydrates formed in natural sands.

In this work, the decomposition behaviour of methane hydrate in porous media was investigated microscopically using powder X-ray diffraction, cryogenic scanning electron microscopy and Raman spectroscopy. The effect of grain sizes on the decomposition of methane hydrate was measured. The results showed that bulk hydrates could exist stably at 223 K and atmospheric pressure because of the self-preservation effect.