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Article Abstract
Objective: To explore the effect of microRNA (miR)-192-5p on the inflammatory and fibrotic responses of tendon cells.
Methods: Tendon cells were treated with transforming growth factor-1 (TGF-1). The expression of miR-192-5p and nuclear factor of activated T cells 5 (NFAT5) in tendon cells were detected by RT-qPCR. The expressions of inflammatory and fibrosis-related factors were detected by RT-qPCR and Western blot. MiR-192-5p binds to NFAT5 targeting by TargetScan and dual-luciferase reporter gene assay. The expression of the NFAT5 gene was detected by RT-qPCR and Western blot. Detection of apoptosis in tendon cells by flow cytometry.
Results: MiR-192-5p was downregulated in tendon cells, and the expression level gradually decreased with the prolong of TGF-1 treatment. The expression of NFAT5 increased with the treatment time of TGF-1. The expression of miR-192-5p decreased collagen III (COLIII), smooth muscle actin (-SMA), matrix metalloproteinase- (MMP-) 1, and MMP-8 expression, thereby inhibiting TGF-1-induced fibrosis in tendon cells. The expression of miR-192-5p decreased the expression of tumor necrosis factor- (TNF-), interleukin (IL)-6, and IL-1, thereby alleviating TGF-1-induced inflammatory response and reduce apoptosis in tendon cells. NFAT5 is a direct target of miR-192-5p in tendon cells. The upregulation of NFAT5 reversed the effect of miR-192-5p on the fibrotic activity and inflammatory response of TGF-1-stimulated tendon cells.
Conclusions: MiR-192-5p alleviates fibrosis and inflammatory responses of tendon cells by targeting NFAT5.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276496 | PMC |
| http://dx.doi.org/10.1155/2022/6481846 | DOI Listing |
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