Resistome and microbiome shifts in catfish rearing water: the influence of temperature and antibiotic treatments.

The increasing reliance on aquaculture for sustainable protein production highlights the need for responsible antibiotic use to manage bacterial infections, particularly in intensive farming systems. This study investigated the effects of three FDA-approved antibiotics (Aquaflor®, Romet®, Terramycin®) at common fish bacterial disease outbreak temperatures (20 °C, 25 °C, and 30 °C) on the microbiome and resistome of aquaculture water using a catfish model system. Metagenomic analyses evaluated the abundance, diversity, and mobility of antimicrobial resistance genes (ARGs) and antibiotic-resistant bacteria (ARB).

The impact of florfenicol treatment on the microbial populations present in the gill, intestine, and skin of channel catfish (Ictalurus punctatus).

Background: Florfenicol is a broad-spectrum antimicrobial approved in many countries for treating bacterial infections in production animals. Although florfenicol has been widely used in the US catfish industry, its impact on the native microbiota within catfish tissues remains largely unknown. Florfenicol treatment is followed by a mandatory withdrawal period to ensure drug residues fall below regulatory limits before harvest.

Incorporating Drought Thresholds Improves Model Predictions of Autumn Phenology in Tropical and Subtropical Forests.

Drought dramatically influences vegetation phenology, thereby impacting terrestrial carbon and water cycles. However, the mechanisms by which drought drives changes in autumn phenology remain unclear, hindering the accurate simulation of these processes in phenology models. In this study, we employed ridge regression analysis to quantify the dynamic effects of intensifying drought on the end-of-photosynthetic-growing-season (EOPS) and identified the drought threshold at which the vegetation's response to drought shifts.

Survival of Listeria monocytogenes on growing and harvested Trumpet Royale (Pleurotus eryngii), Alba Clamshell (Hypsizygus tessellatus), and Brown Clamshell (Hypsizygus tessellatus) mushrooms.

Outbreaks and recalls of Listeria monocytogenes associated with commercial mushrooms have been reported in recent years. Unfortunately, knowledge about the survival of L. monocytogenes on different mushrooms remains limited.

Effect of Bayer Red Mud on the Mechanical Strength of Grouting Material.

The massive stockpiles of Bayer-process red mud (BRM) severely compromise soil integrity, necessitating the urgent development of efficient large-scale utilization strategies. BRM contains large amounts of calcium, silicon, and aluminum. Theoretically, water glass and flue gas desulfurization gypsum (FGD) can increase the active substances in BRM, making it a cementitious raw material capable of replacing cement.

Microelectricity enhances aerobic granular sludge granulation and sulfamethazine degradation: Performance, mechanism, antibiotic resistance genes and microbial community.

With the widespread use of typical antibiotics such as sulfamethazine (SMT), it leads to their accumulation in the environment, increasing the risk of the spread of antibiotic resistance genes (ARGs). Aerobic granular sludge (AGS) has shown great potential in treating antibiotic wastewater. However, the long cultivation period of AGS, the easy disintegration of particles and the poor stability of degradation efficiency for highly concentrated antibiotic wastewater are still urgent problems that need to be solved, and it is important to explore the migration and changes of ARGs and microbial diversity in AGS systems.

Toward Sustainable Utilization and Production of Tartaric Acid.

Global efforts toward establishing a circular carbon economy have guided research interests towards exploring renewable technologies that can replace environmentally harmful fossil fuel-based production routes. In this context, sugar-based bio-derived substrates have been identified as renewable molecules for future implementation in chemical industries. Tartaric acid, a special C bio-compound with two hydroxyl and carboxylic groups in the structure, displays great potential for the food, polymer, and pharmaceutical industries due to its unique biological reactivity and performance-enhancing properties.

Development of an instrument to measure the competencies of health professionals in the process of evidence-based healthcare: A Delphi study.

Aims: To identify and reach consensus on dimensions and criteria of a competence assessment instrument for health professionals in relation to the process of evidence-based healthcare.

Design: A two-round Delphi survey was carried out from April to June 2023.

Methods: Consensus was sought from an expert panel on the instrument preliminarily established based on the JBI Model of Evidence-Based Healthcare and a rapid review of systematic reviews of relevant literature.

Warming increases the differences among spring phenology models under future climate change.

Phenological models are built upon an understanding of the influence of environmental factors on plant phenology, and serve as effective tools for predicting plant phenological changes. However, the differences in phenological model predictive performance under different climate change scenarios have been rarely studied. In this study, we parameterized thirteen spring phenology models, including six one-phase models and seven two-phase models, by combining phenological observations and meteorological data.

Formation of Listeria monocytogenes persister cells in the produce-processing environment.

Persisters are a subpopulation of growth-arrested cells that possess transient tolerance to lethal doses of antibiotics and can revert to an active state under the right conditions. Persister cells are considered as a public health concern. This study examined the formation of persisters by Listeria monocytogenes (LM) in an environment simulating a processing plant for leafy green production.

Development and validation of a questionnaire to evaluate the knowledge, attitude, behaviour and care preference of family members of Chinese older adults related to palliative care.

Aim: To develop and validate a questionnaire on knowledge, attitude, behaviour and care preference of family members of Chinese older adults related to palliative care.

Design: A descriptive study design and STROBE checklist were applied in this research.

Methods: The theoretical framework of the questionnaire was knowledge-attitude-behaviour model.

Consumption of common illicit drugs in twenty-one cities in southwest China through wastewater analysis.

Wastewater-based epidemiology (WBE) was applied to estimate illicit drugs consumption at a provincial scale in southwest China. A large-scale wastewater sampling campaign was carried out from October to November in 2021 in 156 different wastewater treatment plants (WWTPs). Two 24-h composite influent wastewater samples were collected in each WWTP.

Genome-Wide Identification and Transcriptional Expression Profiles of in the Barley ( L.) Pan-Genome.

The gene family protein phosphatase 2C (PP2C) is related to developmental processes and stress responses in plants. Barley ( L.) is a popular cereal crop that is primarily utilized for human consumption and nutrition.

Resolving the Dust Bowl paradox of grassland responses to extreme drought.

During the 1930s Dust Bowl drought in the central United States, species with the C photosynthetic pathway expanded throughout C-dominated grasslands. This widespread increase in C grasses during a decade of low rainfall and high temperatures is inconsistent with well-known traits of C vs. C pathways.

[Efficacy of Autologous DC-CIK Cells Combined with Other Immune Cells in the Treatment of Hematological Malignancies -Retrospective Study].

Objective: To investigate the safety and clinical efficacy of autologous DC-CIK cells combined with other immune cells for patients with hematological malignancies and analyze patient prognosis.

Methods: 50 patients with hematological malignancies who received cellular immunotherapy from September 2014 to April 2016 were retrospectively studied in the First Affiliated Hospital of Xi'an Jiaotong University, 115 cases times of cellular immunotherapy were performed. According to the selected treatment, the patients were divided into the dual cell group (DC-CIK cell treatment) and the multi-cell group (DC-CIK cell combined with other immune cells); According to the treatment course, the patients were divided into the single course group (completed by ＜3 times) and the multiple course group.

Platinum-Ion-Mediated Self-Assembly of Hairpin Peptides and Synthesis of Platinum Nanostructures.

Nanostructures and nanomaterials based on peptide self-assembly have attracted tremendous interests due to the functionalities of peptide molecules. Furthermore, the self-assembled peptide nanostructures are also adopted to fabricate nanomaterials and nanodevices. In this work, the intramolecular folding and self-assembly of a β-hairpin peptide CBHH were first studied under the regulation of platinum ion.

A general gelation strategy for 1D nanowires: dynamically stable functional gels for 3D printing flexible electronics.

3D printing of functional inorganic nanowires has been accomplished using extruded nanowire-based inks obtained by incorporating nanofillers into polymeric matrices or thickeners. However, the presence of inactive additives poses a critical challenge for fully realizing the functionality of the nanowires in the printed structures, which remains a fundamental hurdle to overcome. Here, to construct 3D-printed electronics with high performance, we developed a versatile gelation strategy to obtain thixotropic nanowire gels through formation of dynamically stable 3D networks using small amounts of flexible, water-soluble and single-layer 2D nanosheets, such as graphene oxide and MXene, as physical cross-linkers.

High expression of type I inositol 1,4,5-trisphosphate receptor in the kidney of rats with hepatorenal syndrome.

Aim: To detect the expression of type I inositol 1,4,5-trisphosphate receptor (IPRI) in the kidney of rats with hepatorenal syndrome (HRS).

Methods: One hundred and twenty-five Sprague-Dawley rats were randomly divided into four groups to receive an intravenous injection of D-galactosamine (D-GalN) plus lipopolysaccharide (LPS; group G/L, = 50), D-GalN alone (group G, = 25), LPS alone (group L, = 25), and normal saline (group NS, = 25), respectively. At 3, 6, 9, 12, and 24 h after injection, blood, liver, and kidney samples were collected.

GC-MS Analysis of the Volatile Constituents in the Leaves of 14 Compositae Plants.

The green organs, especially the leaves, of many Compositae plants possess characteristic aromas. To exploit the utility value of these germplasm resources, the constituents, mainly volatile compounds, in the leaves of 14 scented plant materials were qualitatively and quantitatively compared via gas chromatography-mass spectrometry (GC-MS). A total of 213 constituents were detected and tentatively identified in the leaf extracts, and terpenoids (especially monoterpene and sesquiterpene derivatives), accounting for 40.

Dynamic control of the mevalonate pathway expression for improved zeaxanthin production in Escherichia coli and comparative proteome analysis.

Engineered heterologous multi-gene metabolic pathways often suffer from flux imbalance and toxic metabolites, as the production host typically lacks the regulatory mechanisms for the heterologous pathway. Here, we first coordinated the expression of all genes of the mevalonate (MEV) pathway from Saccharomyces cerevisiae using the tunable intergenic regions (TIGRs), and then dynamically regulated the TIGR-mediated MEV pathway to prevent the accumulation of toxic metabolites by using IPP/FPP-responsive promoter. After introduction of the dynamically controlled TIGR-mediated MEV pathway into Escherichia coli, the content and concentration of zeaxanthin in shaker flask cultures were 2.

CmWRKY15 Facilitates Alternaria tenuissima Infection of Chrysanthemum.

Abscisic acid (ABA) has an important role in the responses of plants to pathogens due to its ability to induce stomatal closure and interact with salicylic acid (SA) and jasmonic acid (JA). WRKY transcription factors serve as antagonistic or synergistic regulators in the response of plants to a variety of pathogens. Here, we demonstrated that CmWRKY15, a group IIa WRKY family member, was not transcriptionally activated in yeast cells.