Metabolomics analysis and mRNA/miRNA profiling reveal potential cardiac regulatory mechanisms in Yili racehorses under different training regimens.

Yili horses, a versatile breed from Xinjiang, China, are renowned for their racing abilities. However, studies on the links between cardiac morphology, function, and metabolic profiles with performance are limited. This study combined echocardiographic, transcriptomic, and metabolomic analyses to explore these relationships in high-level, average, and untrained Yili horses.

1D/3D MOF-919-Sc Networked Octahedron-Derived 2D/3D ScO@CuO Layered Octahedron for Polarity-Switchable Photoelectrochemical Sensing.

Engineering a multidimensional polarity-switchable material to achieve the full range of transportation and comprehensive benefits is highly expected, which should provide an advanced switcher for sensing. This work, for the first time, reports the synthesis of the 2D/3D ScO@CuO layered octahedron through simple carbonization of a 1D/3D scandium-doped MOF-919 (MOF-919-Sc) networked octahedron. Based on the as-synthesized 2D/3D ScO@CuO layered octahedron-induced photocurrent-polarity switching of a TiO dodecahedron, a novel polarity-switchable photoelectrochemical (PEC) sensor is constructed for the detection of kanamycin (Kana), combined with target-triggered enzyme cleavage-assisted signal amplification.

Congrong Shujing Granules ameliorates mitochondrial associated membranes to against MPP-induced neurological damage in the cellular model of Parkinson's disease.

Context: Congrong-Shujing Granules (CRSJG) are known to protect dopaminergic neurons in Parkinson's disease (PD), and the mechanism may be related to the improvement of mitochondria-associated membranes (MAMs).

Objective: To investigate the impact of CRSJG-medicated serum on MAMs in the 1-methyl-4-phenylpyridinium (MPP)-induced PD cell model.

Materials And Methods: Human neuroblastoma (SH-SY5Y) cells were treated with 1,000 μmol/L MPP for 24 h, resulting in three experimental groups: MPP, CRSJG, and 2-APB.

Genome-wide association study reveals as a novel susceptibility gene associated with primary spontaneous pneumothorax in the Chinese Han population.

Background: Primary spontaneous pneumothorax is a rare disease commonly found in young adults with unknown etiology. We aimed to investigate the susceptibility genes and the downstream signaling involved in the development of primary spontaneous pneumothorax.

Methods: We conducted the first large-scale genome-wide association study (GWAS) composed of 2223 patients and 3838 controls.

Effects of Different Stages of Training on the Intestinal Microbes of Yili Horses Analyzed Using Metagenomics.

: The aim of this study was to investigate the effects of different stages of training on the intestinal microbial abundance of Yili horses. : Ten Yili horses, all aged 2 years old and weighing 305 ± 20 kg, were selected and divided into a training group and an untrained group. The training group performed riding training 6 days a week, and the untrained group moved freely in the activity circle every day.

One-pot synthesis of enhanced dye-sensitized persistent luminescence nanoparticles to alleviate concentration quenching.

ZnGaO:Cr (ZGC) persistent luminescence (PersL) nanoparticles (PLNPs) are extensively researched in the fields of bioimaging and therapy due to their simple preparation and uniform morphology. Typically, the luminescence intensity increases with the Cr concentration of luminescent ions up to an optimal range of 0.4-0.

Hierarchically amorphous cellulose acetate porous membranes with spectral selectivity for all-weather radiative cooling.

Passive radiative cooling has emerged as a promising approach for cooling without energy consumption. However, developing all-weather passive radiative cooling materials in response to diverse weather conditions remains a significant challenge, since such materials must simultaneously exhibit semi-emissive and transparent properties in the mid-infrared spectrum and high reflectivity to sunlight. Herein, we present a hierarchically amorphous cellulose acetate porous membranes (HAPM), fabricated using a solvent-template-assisted evaporation-induced phase separation (ST-EIPS) strategy, for all-weather passive radiative cooling.

The Impact of the Competition on miRNA, Proteins, and Metabolites in the Blood Exosomes of the Horse.

Purpose: Horse racing may cause stress-induced physiological changes and tissue damage in horses, but the changes in miRNA expression, protein expression, and metabolic substances in the plasma exosomes of the horse after racing are still unclear. This study detected miRNA, protein expression, and metabolic substances in the plasma exosomes of horses before and after competition, providing new insights for post-race recovery and care of horses.

Method: Eight three-year-old horses that had undergone training were selected as the research subjects, with four horses that had not competed as the control group and four horses that had participated in the competition for half an hour as the training group.

CRISPR/Cas13a-Programmed Cu NCs and -Scheme T-COF/AgS for Photoelectrochemical Biosensing of circRNA.

Circular RNAs (circRNAs), as a class of noncoding RNA molecules with a circular structure exhibit high stability and spatiotemporal-specific expression, making them ideal cancer biomarkers for liquid biopsy. Herein, a new photoelectrochemical (PEC) biosensor for a highly sensitive circRNA assay in the whole blood of lung cancer patients was designed based on CRISPR/Cas13a-programmed Cu nanoclusters (Cu NCs) and a -scheme covalent organic framework/silver sulfide (T-COF/AgS) composite. This -scheme T-COF/AgS composite accelerates electron transfer and produces an excellent initial photocurrent.

miR-199a-3p mitigates simulated microgravity-induced cardiac remodeling by targeting MEF2C.

Microgravity-induced cardiac remodeling and dysfunction present significant challenges to long-term spaceflight, highlighting the urgent need to elucidate the underlying molecular mechanisms and develop precise countermeasures. Previous studies have outlined the important role of miRNAs in cardiovascular disease progression, with miR-199a-3p playing a crucial role in myocardial injury repair and the maintenance of cardiac function. However, the specific role and expression pattern of miR-199a-3p in microgravity-induced cardiac remodeling remain unclear.

Enhancing Gpx1 palmitoylation to inhibit angiogenesis by targeting PPT1.

The significance of protein S-palmitoylation in angiogenesis has been largely overlooked, leaving various aspects unexplored. Recent identification of Gpx1 as a palmitoylated protein has generated interest in exploring its potential involvement in novel pathological mechanisms related to angiogenesis. In this study, we demonstrate that Gpx1 undergoes palmitoylation at cysteine-76 and -113, with PPT1 playing a crucial role in modulating the depalmitoylation of Gpx1.

CRISPR-Enhanced Photocurrent Polarity Switching for Dual-lncRNA Detection Combining Deep Learning for Cancer Diagnosis.

Abnormal expression in long noncoding RNAs (lncRNAs) is closely associated with cancers. Herein, a novel CRISPR/Cas13a-enhanced photocurrent-polarity-switching photoelectrochemical (PEC) biosensor was engineered for the joint detection of dual lncRNAs, using deep learning (DL) to assist in cancer diagnosis. After target lncRNA-activated CRISPR/Cas13a cleaves to induce DNAzyme bidirectional walkers with the help of cofactor Mg, nitrogen-doped carbon-Cu/CuO octahedra are introduced into the biosensor, producing a photocurrent in the opposite direction of CdS quantum dots (QDs).

Ckip-1 3'UTR alleviates prolonged sleep deprivation induced cardiac dysfunction by activating CaMKK2/AMPK/cTNI pathway.

Sleep deprivation (SD) has emerged as a critical concern impacting human health, leading to significant damage to the cardiovascular system. However, the underlying mechanisms are still unclear, and the development of targeted drugs is lagging. Here, we used mice to explore the effects of prolonged SD on cardiac structure and function.

A signal-switchable photoelectrochemical biosensor for ultrasensitive detection of long non-coding RNA in cancer cells.

Long non-coding RNA (LncRNA) as an emerging tumor biomarker plays a key factor in the early diagnosis of cancer. Herein, an innovative signal-switchable photoelectrochemical (PEC) biosensor based on ZrO@CuO bimetallic oxides and T7 Exo-assisted signal amplification is reported for the ultrasensitive and selective detection of lncRNA (HOX gene antisense intergenic RNA, HOTAIR) in cancer cells. Firstly, MOFs-derived TiO nanodisks as an excellent photoactive material show an anodic background signal.

Simulated spaceflight-induced cardiac remodeling is modulated by gut microbial-derived trimethylamine N-oxide.

Spaceflight is physically demanding and can negatively affect astronauts' health. It has been shown that the human gut microbiota and cardiac function are affected by spaceflight and simulated spaceflight. This study investigated the effects of the gut microbiota on simulated spaceflight-induced cardiac remodeling using 10° of head-down bed rest (HDBR) in rhesus macaques and 30° of hindlimb unloading (HU) in mice.

Vascular smooth muscle cell-specific miRNA-214 deficiency alleviates simulated microgravity-induced vascular remodeling.

The human cardiovascular system has evolved to accommodate the gravity of Earth. Microgravity during spaceflight has been shown to induce vascular remodeling, leading to a decline in vascular function. The underlying mechanisms are not yet fully understood.

HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formation.

Bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and osteoblast function play critical roles in bone formation, which is a highly regulated process. Long noncoding RNAs (lncRNAs) perform diverse functions in a variety of biological processes, including BMSC osteogenic differentiation. Although several studies have reported that HOX transcript antisense RNA (HOTAIR) is involved in BMSC osteogenic differentiation, its effect on bone formation in vivo remains unclear.

MOFs-derived CoO@MnO@Carbon dots with enhanced nanozymes activity for photoelectrochemical detection of cancer cells in whole blood.

Nanozymes have attracted widespread attention, and rationally designing high-activity nanozymes to improve their application performance are a long-term objective. Herein, taking metal-organic frameworks-derived CoO polyhedron with large surface area and high porosity as nanoconfinement carriers, CoO@MnO@CDs polyhedron was successfully synthesized by the room-temperature reduction of MnO ions and physical load of carbon dots (CDs). Through cancer cells-triggered double antibody sandwich strategy, the CoO@MnO@CDs polyhedron were introduced to the TiO nanoparticle (NPs) modified electrode, leading to the decreased photocurrent.

A dual-model immobilization-free photoelectrochemical/visual colorimetric bioanalysis based on microemulsion self-assemblies mediated multifunctional signal amplification strategy.

Herein, a novel silver ion-loaded gold microemulsion assemblies (Au/Ag MAs) mediated multifunctional signal amplification strategy was proposed to construct a sensitive immobilization-free photoelectrochemical (PEC)/colorimetric biosensor for carcinoembryonic antigen (CEA) detection. Through the sandwiched reaction among CEA, the CEA aptamer (DNA1) loaded on the Au nanoparticles (NPs) functionalized iron oxide (FeO) nanospheres and another CEA aptamer (DNA2) immobilized on Au/Ag MAs, a complex is formed and acquired by magnetic separation. Then, Au/Ag MAs of the complex are disassembled into Au NPs and Ag ions driven by an acetone response, and the obtained demulsification solution is transferred to the cadmium sulfide/cadmium telluride (CdS/CdTe) photoactive composites modified electrode.

Ultrasensitive iodide detection in biofluids based on hot electron-induced reduction of p-Nitrothiophenol on Au@Ag core-shell nanoparticles.

Surveillance of iodine intake is important because either inadequate or excessive amount of iodine may lead to thyroid malfunctions. Herein, we report a method for fast iodide quantification based on a plasmonic hot electron-driven chemical reaction, which occurs on Au@Ag core-shell nanoparticles (NPs) coated with p-nitrothiophenol (PNTP) molecules. Upon resonant light illumination, hot electron-hole pairs are generated in the NPs.

Recognition of an Ala-rich C-degron by the E3 ligase Pirh2.

The ribosome-associated quality-control (RQC) pathway degrades aberrant nascent polypeptides arising from ribosome stalling during translation. In mammals, the E3 ligase Pirh2 mediates the degradation of aberrant nascent polypeptides by targeting the C-terminal polyalanine degrons (polyAla/C-degrons). Here, we present the crystal structure of Pirh2 bound to the polyAla/C-degron, which shows that the N-terminal domain and the RING domain of Pirh2 form a narrow groove encapsulating the alanine residues of the polyAla/C-degron.

Mechanical stimulation controls osteoclast function through the regulation of Ca-activated Cl channel Anoctamin 1.

Mechanical force loading is essential for maintaining bone homeostasis, and unloading exposure can lead to bone loss. Osteoclasts are the only bone resorbing cells and play a crucial role in bone remodeling. The molecular mechanisms underlying mechanical stimulation-induced changes in osteoclast function remain to be fully elucidated.

Plasma Non-targeted Metabolomics Analysis of Yili Horses Raced on Tracks With Different Surface Hardness.

In this study, the plasma non-targeted metabolomics of Yili horses were characterized before and after exercise on tracks that differed in surface hardness to better understand exercise-related biochemical changes. Blood samples were obtained from eight trained Yili horses before and immediately after exercise. Samples were used for metabolomic analysis by ultra-performance liquid chromatography-Q-EXACTIVE mass spectrometry.

Osteoblast Derived Exosomes Alleviate Radiation- Induced Hematopoietic Injury.

As hematopoietic stem cells can differentiate into all hematopoietic lineages, mitigating the damage to hematopoietic stem cells is important for recovery from overdose radiation injury. Cells in bone marrow microenvironment are essential for hematopoietic stem cells maintenance and protection, and many of the paracrine mediators have been discovered in shaping hematopoietic function. Several recent reports support exosomes as effective regulators of hematopoietic stem cells, but the role of osteoblast derived exosomes in hematopoietic stem cells protection is less understood.