The role of LncRNA TCONS_00058979 in the inflammatory response of LPS-induced bovine mammary epithelial cells.

This study aimed to elucidate the functional mechanisms and molecular regulatory pathways of long non-coding RNA TCONS_00058979 in bovine mastitis. By establishing a lipopolysaccharide (LPS)-induced inflammatory model in bovine mammary epithelial cells (bMECs), we assessed cell viability, proliferation, and apoptosis using CCK-8 assay, EdU labeling, and flow cytometry, respectively, and predicted potential interacting proteins through RNA pull-down assays combined with bioinformatics analysis. Results showed that knockdown of lncRNA TCONS_00058979 significantly enhanced viability and proliferation, inhibited apoptosis, and downregulated mRNA and protein expression of inflammatory cytokines IL-1β, IL-6, and IL-8 in LPS-treated bMECs, while overexpression exhibited opposite effects.

YTHDF2-mediated mA modification regulates mRNA stability of to modulate cell death in induced bovine mastitis.

Background: ()-induced bovine mastitis is a major challenge for dairy production, causing significant economic losses. The regulatory mechanisms underlying host cell apoptosis and inflammation during infection remain unclear. Therefore, this study investigates the role of N6-methyladenosine (mA) modification and its reader protein YTHDF2 in regulating mRNA stability, apoptosis, and inflammation in bovine mammary epithelial cells (Mac-T cells) under challenge.

Transcriptome profiling of mRNA responding to inflammation in bovine mammary epithelial cells induced by lipopolysaccharide.

Bovine mastitis, caused by pathogen infection in mammary tissue, incurs significant economic losses. Bovine mammary epithelial cells (bMECs) act as both lactation organs and primary defense against pathogens. Lipopolysaccharide (LPS), a virulence factor of Escherichia coli (E.

Miniaturized Tunable Laser Spectrometer for the Simultaneous Detection of Water Ice and Hydrogen-Oxygen Isotopes for the Chang'E-7 Lunar Soil Water Molecule Analyzer.

Distinguishing the origin of lunar water ice requires in situ isotopic measurements with high sensitivity and robustness under extreme lunar conditions; however, challenges such as uncertain water contents and isotopic fractionation induced by regolith particles restrict isotopic analysis. Herein, we present a miniaturized tunable diode laser absorption spectrometer (TDLAS) developed as the core prototype for the Chang'E-7 Lunar Soil Water Molecule Analyzer (LSWMA). The wavelength range of the instrument is 3659.

A wireless and passive RFID tag sensor for the detection of Pb in soil combining chemiresistive sensing and impedance mismatch: A new method for onsite detection of hazardous metals.

In this work, a wireless and passive radio frequency identification (RFID) tag sensor, which integrated a reduced graphene oxide/ion-selective membrane (rGO/ISM) chemiresistive sensing component, was developed for the onsite detection of Pb in soil. Additionally, a new detection method combining impedance mismatch and spectral sensing technique for Pb was proposed. Furthermore, the sensing mechanism of the RFID tag sensor was investigated in terms of the field-effect transistor (FET) transfer curve and antenna reflection coefficient.

The Platelet Activation Signaling Pathway Regulated by Fibrinogen and Homo-Gamma-Linolenic Acid (C20:3)-Associated Lipid Metabolism Is Involved in the Maintenance of Early Pregnancy in Chinese Native Yellow Cattle.

Identifying the specific factors secreted during early pregnancy is an effective method for pregnancy detection in cattle, helping to reduce empty pregnancies in the industry. To systematically investigate metabolic variations between early pregnancy and the estrous cycle and their relationship with pregnancy progression, this study utilized four-dimensional data-independent acquisition (4D-DIA) proteomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomics to analyze serum samples collected from Chinese native yellow cattle at day 0 and day 21 post-mating, combining bioinformatics analysis with experimental validation. The platelet activation signaling pathway and angiogenesis-related proteins were significantly upregulated.

Proteomics and metabolomics reveal the role of miR-320b in regulating inflammation of bovine mammary epithelial cells.

MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that are widely found in organisms and play an important regulatory role in various biological processes, especially immune and inflammatory responses. However, the function of miR-320b in the inflammatory responses of bovine mammary epithelial cells (bMECs) remains to be elucidated. In this study, we examined the miR-320b mimic transduction group (miR-320b_mimic) and negative control mimic transduction group (NC_mimic) of lipopolysaccharide-treated bMECs using data-independent acquisition (DIA) proteomics and untargeted metabolomics.

Label-free quantitative proteomics analysis of bovine mammary glands induced with Escherichia coli.

Objective: To establish a model of Escherichia coli-induced bovine mammary gland inflammation and investigate alterations in protein expression in bovine mammary glands under different health conditions.

Methods: 6 Chinese Holstein cows were selected and randomly assigned to 2 groups. Bovine mammary glands were induced with either 105 CFU/mL E coli (n = 3) or 1 X PBS (n = 3), and label-free quantitative proteomics was applied to explore the effect of E coli on the expression of proteins in bovine mammary glands.

Analysis of Differentially Expressed Proteins in Bovine Mammary Glands Infected with .

Mastitis is among the most prevalent diseases in dairy cows, leading to substantial issues such as decreased milk yield and quality, reduced reproductive performance, early culling, and increased production costs. , a naturally occurring opportunistic pathogen, is majorly responsible for mastitis in dairy cows. Mammary tissues from healthy cows were used as a control group (M_C, = 3), and mammary glands of Chinese Holstein cows infected with (10 colony-forming unit/mL) were used as experimental groups (M_S, = 3).

Profile of circular RNAs in bovine mammary tissues infected with Staphylococcus aureus.

Mastitis is one of the most common diseases in cattle. The causes are complex, and the disease tends to recur and its difficult to cure, resulting in significant economic losses to the global dairy industry each year. Circular RNAs (circRNAs), a class of endogenous non-coding RNAs (ncRNAs), have recently been recognized as key regulators in inflammatory diseases.

Identification of potential key circular RNAs associated with Escherichia coli-infected bovine mastitis using RNA-sequencing: preliminary study results.

Escherichia coli (E. coli) is commonly found in dairy farms and can invade mammary gland tissue, often causing acute clinical mastitis. Mammary infections with E.

LncRNA HULIB promotes LPS induced inflammatory response in bovine mammary epithelial cells via PP2AB.

Bovine mastitis is regulated by genetic and environmental factors. Long non-coding RNAs (LncRNAs), which regulate various biological processes (immune system and biological development), have been found to play a role in bovine mammary inflammation responses. Here, a novel functional lncRNA, named lncRNA HULIB, was identified as a regulator during bovine mastitis.

Transcriptome analysis reveals the regulation of miR-19b on inflammation in bovine mammary epithelial cells.

MicroRNAs (miRNAs) are involved in various biological processes where they regulate the expression of mRNAs. Bovine mammary epithelial cells (bMECs) are functional cells that mediate mammary inflammatory immunity. Although numerous miRNAs regulate the function of bMECs, the role of miR-19b in bMECs has not been reported.

The role of microRNAs in the regulation of critical genes and signalling pathways that determine endometrial receptivity.

Endometrial receptivity is the ability of the endometrium to accept embryos. Thus, endometrial receptivity dysfunction is an important factor leading to embryo implantation failure. A good endometrial receptivity provides a suitable environment for embryo implantation, improving the embryo implantation rate.

Matching comparative advantages to special economic zones for sustainable industrialization.

African countries are consistently trying to leverage industrialization to advance their economic development. Despite possessing favorable factors such as abundant natural resources, a surplus of low-cost labor, and an increasing number of established Special Economic Zones (SEZs), African countries are yet to fully maximize their potential and achieve sustainable and inclusive industrialization. This study explores the comparative advantages of African countries to determine the types of SEZs that can effectively accelerate industrialization.

LncRNA CA12-AS1 targets miR-133a to promote LPS-induced inflammatory response in bovine mammary epithelial cells.

Bovine mastitis seriously affects milk production and quality and causes huge economic losses in the dairy industry. Recent studies have shown that long non-coding RNAs (lncRNAs) may regulate bovine mastitis. In this study, the expression of lncRNA CA12-AS1 was significantly upregulated in LPS-induced bovine mammary epithelial cells (bMECs) but negatively correlated with the expression of miR-133a, suggesting that it may be related to the inflammatory response in bMECs.

Simulation and prediction of changes in maximum freeze depth in the source region of the Yellow River under climate change.

The source region of the Yellow River (SRYR) is located at the edge of the Qinghai-Tibet Plateau (QTP), which is completely covered by frozen ground. Due to relatively higher temperatures, the frozen ground in the SRYR is particularly fragile and susceptible to the impacts of global climate change. This study discusses the maximum freeze depth (MFD) of frozen ground in the SRYR, including analysis of measured data at the stations, comparison of simulation models, and projection of future changes.

Correction: Jiao et al. Bta-miR-223 Targeting the RHOB Gene in Dairy Cows Attenuates LPS-Induced Inflammatory Responses in Mammary Epithelial Cells. 2022, , 3144.

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Bta-miR-199a-3p Inhibits LPS-Induced Inflammation in Bovine Mammary Epithelial Cells via the PI3K/AKT/NF-κB Signaling Pathway.

Mastitis is characterized by inflammatory damage to mammary gland tissue, which could decline milk production and quality and significantly affect the economic benefits of ranching. MicroRNAs (miRNAs), such as miR-199a-3p, are novel therapeutic targets in inflammation, and their regulation is an effective strategy for inflammation control. Despite its importance in humans and animals, the molecular mechanism of bovine miR-199a-3p (bta-miR-199a-3p) in dairy cow mastitis and bovine mammary epithelial cell (bMEC) inflammation is unclear.

RNA-seq reveals the role of miR-199a-3p in regulating inflammation of bovine mammary epithelial cells.

MicroRNAs (miRNAs) are involved in the regulation of a variety of biological processes. However, the research on the regulatory role of bovine mammary epithelial cells (bMECs) is scarce. To date, there are no reports about the role of miR-199a-3p in bMECs.

Bta-miR-223 Targeting the RHOB Gene in Dairy Cows Attenuates LPS-Induced Inflammatory Responses in Mammary Epithelial Cells.

Bovine mammary epithelial cells (bMECs) are part of the first line of defense against pathogens. In recent studies, bta-miR-223 has been reported to activate congenital and innate immunity against inflammatory damage during the pathogenesis of mastitis in dairy cows. The purpose of this study was to identify the regulatory mechanism of bta-miR-223 and its downstream target genes in inflammatory bMECs.