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Article Abstract
Background: ()-induced bovine mastitis is a major challenge for dairy production, causing significant economic losses. The regulatory mechanisms underlying host cell apoptosis and inflammation during infection remain unclear. Therefore, this study investigates the role of N6-methyladenosine (mA) modification and its reader protein YTHDF2 in regulating mRNA stability, apoptosis, and inflammation in bovine mammary epithelial cells (Mac-T cells) under challenge.
Methods: MeRIP-seq, RIP-seq, and RT-qPCR were used to analyze mA-modified mRNA and its interaction with YTHDF2. Apoptosis, necrosis, and mitochondrial function were assessed using YO-PRO-1/PI staining and JC-1 assays.
Results: infection significantly downregulated expression in Mac-T cells, leading to destabilization of mA-modified mRNA. This resulted in increased reactive oxygen species (ROS) levels, mitochondrial dysfunction, and cell apoptosis. Overexpression of restored mRNA stability, reduced apoptosis, and preserved mitochondrial function.
Conclusion: YTHDF2 regulates mA-modified mRNA stability to modulate apoptosis and inflammation during infection. These findings provide new insights into understanding the molecular mechanisms of bovine mastitis and provide genetic markers for breeding mastitis-resistant dairy cows.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162568 | PMC |
| http://dx.doi.org/10.3389/fcimb.2025.1542647 | DOI Listing |
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