Evidence for trans-generational immune priming against in the oyster .

Cumulative evidence have demonstrated the occurrence of trans-generational immune priming (TGIP) in invertebrates; however, the detailed substances transferred, and the mechanism of this transmission remain unclear. In the present study, we first tested TGIP in the offspring of after parental challenge with during the spawning season. In the maternal oyster primed with , the enzyme activities (lysozyme and SOD), NO level, the expression of immune genes (, , and ), as well as the antibacterial activities were significantly enhanced in the eggs of -primed female oysters, indicating that stimulation promoted the immunity tendentiously transferred to eggs during the spawning season.

Emerging role of lipid droplets in obscure puffer immune response against .

Unlabelled: As dynamic and functionally active organelles, lipid droplets (LDs) mainly function in lipid anabolism, while recent studies showed that mammalian LDs also actively participated in innate immunity; however, the specific roles and regulation mechanism remain relatively unexplored, and the existing studies were mainly limited to mammals. In the present study, we first found that , a serious pathogen in marine environment, could induce LDs accumulation in the liver of obscure puffer on the histology, morphology and molecular levels, and the induction mainly conducted by promoting the synthesis of neutral lipids. Moreover, the antibacterial activity of LD proteins was significantly enhanced upon stimulation, and showed broad-spectrum characteristic.

Metabolic syndrome is associated with worse prognosis in elderly patients with hepatocellular carcinoma.

Background: Metabolic syndrome (MetS), a constellation of metabolic abnormalities such as obesity, hypertension, dyslipidemia, and insulin resistance, has been implicated in cancer progression. However, its impact on the prognosis of hepatocellular carcinoma (HCC) in elderly patients remains unclear. This study evaluates the relationship between MetS and survival outcomes in elderly patients undergoing hepatectomy for HCC.

Type I interferon (IFNd) enhanced the innate immune response and lipid droplets (LDs) formation in obscure puffer Takifugu obscurus.

As one member of type I IFN, IFNd showed huge distinctive responses and activity during the viral or bacterial infection in various fish species. Our previous study identified IFNd from obscure puffer Takifugu obscurus (ToIFNd), and the function and regulation of ToIFNd was further investigated in the present study. The transcriptional levels of ToIFNd were significantly induced post bacteria Vibrio harveyi and virus simulator Poly (I:C) stimulation in the head kidney.

derived outer membrane vesicles mediated bacterial virulence, antibiotic resistance, host immune responses and clinical applications.

is a gram-negative pathogen that can cause multiple diseases including sepsis, urinary tract infections, and pneumonia. The escalating detections of hypervirulent and antibiotic-resistant isolates are giving rise to growing public concerns. Outer membrane vesicles (OMVs) are spherical vesicles containing bioactive substances including lipopolysaccharides, peptidoglycans, periplasmic and cytoplasmic proteins, and nucleic acids.

Roles of S100A1 and S100A10 from hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) in immune response to Vibrio infection.

The S100 proteins are highly conserved EF-hand calcium-binding proteins found only in vertebrates. In the current study, two S100 genes (S100A1 and S100A10) were successfully identified and characterized from hybrid grouper Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀. The deduced S100A10 protein contained two EF-hand domains, and S100A1 only possessed the N-terminal EF-hand.

A preliminary investigation on the protective effects of β-glucan and mannan induced trained immunity in pufferfish Takifugu obscurus.

Immune stimuli are able to trigger long-term protective effects through mechanisms of trained immunity, which has attracted increasing attention. Although the existence of trained immunity has evidenced in teleost fish, while there were no such reports in pufferfish (Takifugu obscurus) so far. Therefore, the present study aimed to evaluate the induction of β-glucan and mannan on the trained immunity and their protective efficacy against Vibrio harveyi re-stimulation in pufferfish.

Identification and characterization of 16 tripartite motif-containing proteins from Takifugu obscurus.

Tripartite motif-containing (TRIM) proteins play important roles in apoptosis, development, autophagy, and innate immunity in vertebrates. In this study, a total of 16 TRIM genes were cloned and identified from obscure puffer Takifugu obscurus. Multiple alignment results showed that most of the deduced ToTRIM proteins contained three typical motifs, a really interesting new gene (RING) zinc-finger domain, one B-box, and a coiled-coil domain, which together formed the TRIM motif found in this large family of proteins.

Folic acid mitigates the developmental and neurotoxic effects of bisphenol A in zebrafish by inhibiting the oxidative stress/JNK signaling pathway.

Bisphenol A (BPA) is a widespread environmental endocrine disruptor (EED) that can cause various environmental and health issues by inducing oxidative stress. The c-Jun N-terminal kinase (JNK) signaling pathway plays a crucial role in oxidative stress-mediated cellular damage. Although folic acid (FA) has demonstrated antioxidant properties, its potential protective effects against BPA-induced developmental and neurotoxicity, as well as the mechanisms involved in the JNK signaling pathway, are still not completely understood.

Description of sp. nov. and proposal of the genera , , and as synonyms of .

Two Gram-stain-negative, aerobic, ovoid to short rod-shaped bacterial strains, designated as WL0062 and WL0115, were isolated from coastal zone of the Yellow Sea, Jiangsu Province, PR China, respectively. Strain WL0062 grew optimally at 28 °C, pH 7.0-8.

Pan-cancer analysis of the role of MPP7 in human tumors.

MAGUK p55 subfamily member 7, a part of the membrane palmitoylated protein subfamily, is an essential adapter that promotes epithelial cell polarity and has increasing significance in multiple cancers, including esophageal cancer, clear cell renal cell carcinoma, breast cancer, and pancreatic ductal adenocarcinoma. This paper aims to determine the effect of the MAGUK p55 subfamily member 7 in various tumor types using The Cancer Genome Atlas and Genotype-Tissue Expression database. A variety of software and web platforms, such as cBioPortal, GEPIA2, TIMER2, UALCAN, R, STRING, and DAVID, were used to obtain and analyze data.

Biodegradable Cascade-Amplified Nanotheranostics for Photoacoustic-Guided Synergistic PTT/CDT/Starvation Antitumor in the NIR-II Window.

The development of nanoassemblies, activated by the tumor microenvironment, capable of generating photothermal therapy (PTT) and amplifying the "ROS (·OH) storm," is essential for precise and effective synergistic tumor treatment. Herein, an innovative cascade-amplified nanotheranostics based on biodegradable Pd-BSA-GOx nanocomposite for NIR-II photoacoustic imaging (PAI) guides self-enhanced NIR-II PTT/chemodynamic therapy (CDT)/starvation synergistic therapy. The Pd-BSA-GOx demonstrates the ability to selectively convert overexpressed HO into strongly toxic ·OH by a Pd/Pd-mediated Fenton-like reaction at a lower pH level.

A multiplex one-step fluorescence quantitative differential diagnosis method for severe hand, foot and mouth disease caused by coxsackievirus A16.

Hand foot and mouth disease (HFMD) is a common childhood infectious disease which is caused by human enterovirus. The objective of this study was to develop a rapid, sensitive, and accurate method for detecting severe HFMD caused by coxsackievirus A16 (CV-A16). A closed-tube sensitive multiplex one-step reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was applied to detect CV-A16 in the early stage of severe HFMD.

Identification of two novel heterozygous variants of SMC3 with Cornelia de Lange syndrome.

Background: Cornelia de Lange syndrome (CdLS) is a multisystem genetic disorder, and cases caused by variants in the structural maintenance of chromosomes protein 3 (SMC3) gene are uncommon. Here, we report two cases of CdLS associated with novel pathogenic variants in SMC3 from two Chinese families.

Methods: Clinical presentations of two patients with CdLS were evaluated, and specimens from the patients and other family members were collected for Trio-based whole-exome sequencing.

The deubiquitinase USP7 and E3 ligase TRIM21 regulate vasculogenic mimicry and malignant progression of RMS by balancing SNAI2 homeostasis.

Background: Rhabdomyosarcoma (RMS) is a rare malignancy and the most common soft tissue sarcoma in children. Vasculogenic mimicry (VM) is a novel tumor microcirculation model different from traditional tumor angiogenesis, which does not rely on endothelial cells to provide sufficient blood supply for tumor growth. In recent years, VM has been confirmed to be closely associated with tumor progression.

Endogenous Melanin and Hydrogen-Based Specific Activated Theranostics Nanoagents: A Novel Multi-Treatment Paradigm for Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a systemic autoimmune disorder characterized by excessive proliferation of rheumatoid arthritis synovial fibroblasts (RASFs) and accumulation of inflammatory cytokines. Exploring the suppression of RASFs and modulation of the RA microenvironment is considered a comprehensive strategy for RA. In this work, specifically activated nanoagents (MAHI NGs) based on the hypoxic and weakly acidic RA microenvironment are developed to achieve a second near-infrared fluorescence (NIR-II FL)/photoacoustic (PA) dual-model imaging-guided multi-treatment.

Relationship between biofilm formation and antibiotic resistance of and updates on antibiofilm therapeutic strategies.

is a Gram-negative bacterium within the family that can cause multiple systemic infections, such as respiratory, blood, liver abscesses and urinary systems. Antibiotic resistance is a global health threat and warrants special attention due to its resistance to most modern day antibiotics. Biofilm formation is a critical obstruction that enhances the antibiotic resistance of .

UBD participates in neutrophilic asthma by promoting the activation of IL-17 signaling.

Neutrophilic asthma is a persistent and severe inflammatory lung disease characterized by neutrophil activation and the mechanisms of which are not completely elucidated. Ubiquitin D (UBD) is a ubiquitin-like modifier participating in infections, immune responses, and tumorigenesis, while whether UBD involves in neutrophilic asthma needs further study. In this study, we initially found that UBD expression was significantly elevated and interleukin 17 (IL-17) signaling was enriched in the endobronchial biopsies of severe asthma along with neutrophils increasing by bioinformatics analysis.

Identification and characterization of an L-type lectin from obscure puffer Takifugu obscurus in response to bacterial infection.

L-type lectins (LTLs) contain a carbohydrate recognition domain homologous to leguminous lectins, and have functions in selective protein trafficking, sorting and targeting in the secretory pathway of animals. In this study, a novel LTL, designated as ToERGIC-53, was cloned and identified from obscure puffer Takifugu obscurus. The open reading frame of ToERGIC-53 contained 1554 nucleotides encoding 517 amino acid residues.

Hollow glass microspheres embedded in porous network of chitosan aerogel used for thermal insulation and flame retardant materials.

In recent years, biopolymer aerogels as thermal insulation materials have received widespread attention due to natural abundance, cost-efficiency, and environment-friendly. However, the flammability and low strength hinder its practical application. Hollow glass microspheres (HGMs) as an inorganic thermal insulation filler have been filled in biopolymer aerogels to improve flame retardancy.

Vapor-etching honeycomb-like zinc plating layer for constructing anti-corrosion lubricant-infused surfaces.

Biomimetic lubricant-infused porous surfaces are developed and applied for omniphobicity and corrosion protection, which exhibit great advantages compared to superhydrophobic surfaces. Herein, superhydrophobic Fe@E-Zn@PFOA was prepared via the electrodeposition of laminated Zinc coating, further vapor etching, and post-modification with perfluoro caprylic acid. The facile, inexpensive, and environment-friendly water vapor etching process can form a porous honeycomb-like structure.