Exploring the multidimensional symptom experience in patients with inflammatory bowel disease-a contemporaneous network analysis.

Aim: To explore and visualize the relationships among multiple symptoms in patients with inflammatory bowel disease (IBD) and present empirical evidence for establishing personalized and precise symptom management strategies.

Design: This is a quantitative research study conducted between May 2024 and March 2025 using a correlational research design.

Methods: A total of 324 individuals diagnosed with IBD and hospitalized in Shanghai completed the Symptom Cluster Scale for Inflammatory Bowel Disease (SCS-IBD).

Comparing predictive accuracy of carotid artery ultrasonography with inferior vena cava ultrasonography for predicting postinduction hypotension in older patients: a prospective observational study.

Objectives: Hypotension frequently occurs following the initiation of general anaesthesia, particularly in older individuals, and is linked to adverse postoperative consequences when it is severe. This study was designed to compare the predictive effects of carotid ultrasound measurements and inferior vena cava (IVC) ultrasound measurements on postinduction hypotension (PIH) in older patients.

Design: Prospective observational study.

Differential plasma proteome analysis reveals key proteins associated with insulin resistance in acne vulgaris patients.

Acne vulgaris (AV) is a chronic inflammatory skin disease with a complex pathogenesis, and its association with insulin resistance (IR) remains unclear. This study aimed to identify differential proteins linking AV and IR to improve diagnostic and therapeutic strategies. Plasma proteomic profiling by using LC-MS/MS was performed on 90 AV patients (with IR [n = 40], without IR [n = 50]) and 30 healthy controls (with IR [n = 11], without IR [n = 19]).

Single-cell perspective on the Monocyte-to-HDL cholesterol ratio as a metastasis biomarker in papillary thyroid cancer.

Background: Papillary thyroid carcinoma (PTC) is a globally widespread inflammation-related cancer, where lymph node metastasis (LNM) poses significant challenges to the prognosis of PTC. The role of the monocyte-to-high-density lipoprotein cholesterol ratio (MHR), a novel inflammation marker attracting increasing attention, in PTC remains unclear.

Methods: Clinical data analysis was adopted to preliminarily explore the relationship between clinical features and LNM of PTC.

GATA8-Mediated Antiviral Defence Is Countered by Tomato Chlorosis Virus-Encoded Pathogenicity Protein p27.

Tomato chlorosis virus (ToCV), a phloem-restricted RNA virus within the genus Crinivirus of the family Closteroviridae, exhibits a broad host range and severely impacts the yield and quality of multiple crops. Viral infection directly alters endogenous phytohormone levels, which are intricately associated with viral mobility, replication, symptom development and defence mechanisms. Previous studies have demonstrated that GATA transcription factors regulate several hormone signalling pathways in plants.

Chinese herbal medicine (Tangshen Formula) formula treatment of patients with diabetic kidney disease: a systematic review and meta-analysis.

Background/objective: Diabetic Kidney Disease (DKD) is a severe complication of diabetes mellitus and is one of the main causes of end-stage renal disease globally. Tangshen Formula (TSF) plays an important role in the treatment of DKD. The purpose of this study was to evaluate the efficacy and safety of TSF compound therapy in treating DKD patients with macroalbuminuria through systematic review and meta-analysis methods.

Pathogenicity factor p22 of tomato chlorosis virus interferes with abscisic acid-mediated antiviral defenses by disrupting the function of WRKY81 in tomato.

Abscisic acid (ABA) is essential for plant resistance to both biotic and abiotic stresses, but its regulation during virus infection and role in antiviral defense remain poorly understood. Here, we report that overexpression of SlWRKY81 led to increased ABA accumulation, whereas silencing SlWRKY81 reduced ABA levels in tomato plants. Further analysis revealed that SlWRKY81 directly binds the promoters of ABA biosynthesis genes SlABA2 and SlABA3, thereby activating their expression.

Quantitative analysis of trends and inequalities in disease burden and care quality of gynecological cancers, 1990-2021.

Background: Cervical (CC), ovarian (OC), and uterine (UC) cancers are major contributors to global gynecological cancer (GC) burden. This research intended to update trends in disease burden and care quality for these cancers and quantify cross-country inequalities between 1990 and 2021.

Methods: We performed a secondary analysis utilizing the Global Burden of Disease 2021 database.

Dietary bile acid supplementation improves the intestinal health and growth performance of piglets partially through the FXR/AQPs pathway.

Background: Maintaining the integrity of the structure and function of piglet intestines is crucial for their growth and health. This study aims to evaluate the effects of an antibiotic free diet supplemented with bile acid on gut health and growth performance of weaned piglets, and to explore their regulatory mechanisms.

Methods: Thirty-two weaned piglets were randomly divided into two groups and fed either a basal diet or a basal diet supplemented with 350 mg/kg bile acid.

Adherence to the planetary health diet is associated with slower cognitive decline: a prospective cohort analysis of Chinese older adults.

Background: The EAT-Lancet commission has proposed a planetary health reference diet (PHD) aiming to improve human health and global environmental sustainability. Emerging evidence suggests that high-quality diet is a modified risk factor cognitive decline. However, population-based evidence in relation to the association between this diet and cognitive decline is scarce.

Lung Tissue Extracellular Vesicles-Mediated Delivery of miR-128-3p as a Novel Mechanism of Acute Lung Inflammation.

Background: Emerging evidence links macrophage overactivation to sepsis-associated acute lung injury (ALI), yet the role of lung tissue-derived extracellular vesicles (Ti-EVs) in this process remains unclear. This study combines transcriptomic profiling and functional validation to reveal how Lung Ti-EVs mediate macrophage polarization through miRNA-dependent NLRP3 inflammasome activation.

Methods: We established a sepsis mouse model, extracted and characterized lung tissue-derived EVs, performed high-throughput transcriptome sequencing and bioinformatics analysis.

Efficacy and safety of ziresovir in hospitalised infants aged 6 months or younger with respiratory syncytial virus infection in China: findings from a phase 3 randomised trial with 24-month follow-up.

Background: Respiratory syncytial virus (RSV) is a particularly dangerous infection in some populations, including very young infants. This study examined the efficacy and safety of ziresovir in hospitalised infants aged 6 months or younger with RSV infection.

Methods: In this double-blind, randomised, placebo-controlled trial conducted across 28 hospitals in China, patients aged 1-24 months hospitalised for virologically confirmed RSV infection were randomly allocated (2:1) to receive ziresovir (10-40 mg by weight twice daily) or placebo orally for 5 days, with 2 years of follow-up.

Immunotherapy-associated autoimmune hemolytic anemia induced by anti-PD-1 therapy in esophageal cancer: A case report and literature review.

Rationale: Numerous immune checkpoint inhibitors have been approved for clinical use in metastatic advanced esophageal cancer. While immunotherapy brings therapeutic benefits, immune-related adverse events (irAEs) should nevertheless not be overlooked. This paper reports on the first documented case of Autoimmune hemolytic anemia (AIHA) caused by anti-programmed cell death protein-1 therapy in esophageal squamous cancer.

Case report: Alectinib-associated intestinal ulceration and colitis in a patient with non-small cell lung cancer and effective treatment with Mesalazine.

Background: Alectinib is effective in extending the survival of patients with anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) and generally has manageable side effects. However, intestinal ulcers and colitis are rare but serious adverse reactions linked to Alectinib, meriting further investigation into their causes.

Case Presentation: We report the case of a 62-year-old woman with NSCLC and brain metastases, who tested positive for ALK.

Transcriptional Profiling of Abomasal Mucosa from Young Calves Experimentally Infected with .

, also known as the brown stomach worm, causes significant pathology in the abomasum, resulting in production and nutritional losses in cattle. Alternative control measures, such as vaccination, are urgently needed because of rapidly growing anthelmintic drug resistance. There is a need to understand host responses to the infection, especially immune responses, to advance vaccine discovery and design.

Thrombospondin-2 induces M2 macrophage polarization through fatty acid metabolism to drive lung adenocarcinoma proliferation.

Tumor-associated macrophages play a critical role in regulating the progression of lung adenocarcinoma (LUAD). Platelet-derived protein thrombospondin-2 (THBS2) has been identified as a tumor marker and is known to be overexpressed in LUAD. However, the specific role of THBS2 in M2 macrophage polarization within LUAD remains unclear.

Multi-responsive Pickering emulsions stabilized by amphiphilic cellulose nanocrystals for building smart release systems of hydrophobic drugs.

In this study, cellulose nanocrystals (CNC) were first extracted from peanut shells. Then, the monomers DMAEMA and MAA were grafted on the surface of CNC using reversible addition-fragmentation chain transfer (RAFT) polymerization to prepare multi-stimuli responsive nanoparticles (CNC/PDM), and they were used to stabilize Pickering emulsions. The effects of such factors as pH, nanoparticle concentration, water-to-oil ratio, and oil polarity on the stability of Pickering emulsions were investigated in detail.

Time-dependent RNA transcriptional profiling of abomasal mucosa in cattle infected with Ostertagia ostertagi.

In this study, we infected Holstein calves with Ostertagia ostertagi stage 3 larvae (L3) and determined gene expression profiles of abomasal fundic and pyloric mucosa by RNA sequencing (RNA-seq) at 3-5, 7-9, 10, and 21 days post-infection (dpi), which represent late L3 and early L4 (between 3-5 dpi), mid to late L4 (between 7-9 and 10 dpi) and adult stages (21 dpi) of the parasitic stage. Bioinformatics analyses were performed to profile the transcriptomic changes over time as well as between cattle abomasal tissues. The results will help understand the gastric responses of the host, especially the immune responses, at different phases of early nematode infection.

The feasibility of the deep hypothermal cryotherapy plus immune checkpoint inhibitor for treatment of severe malignant central airway obstruction.

Background: The recurrence shortly after interventional therapy poses a great challenge in managing malignant central airway obstruction (MCAO). While cryotherapy has shown potential benefits when combined with immunotherapy in lung cancer, its effectiveness for improving local control of malignant central airway tumors is not well understood. This study aims to evaluate the clinical efficacy and safety of combining these strategies.

Profiling Genome-Wide Methylation Patterns in Cattle Infected with .

DNA methylation (DNAm) regulates gene expression and genomic imprinting. This study aimed to investigate the effect of gastrointestinal (GI) nematode infection on host DNAm. Helminth-free Holstein steers were either infected with (the brown stomach worm) or given tap water only as a control.

Genome-Wide Association Analysis of Boar Semen Traits Based on Computer-Assisted Semen Analysis and Flow Cytometry.

Semen quality and persistence are critical for evaluating the usability of individual boars in AI, a standard practice in pig breeding. We conducted GWASs on various semen traits of Duroc boars, including MOT, DEN, ABN, MMP, AIR, and ROS levels. These traits were assessed using FCM and CASA.

N Protein of Tomato Spotted Wilt Virus Proven to Be Antagonistic Against Tomato Yellow Leaf Curl Virus in Nicotiana benthamiana.

Two phylogenetically unrelated viruses transmitted by different insect vectors, tomato spotted wilt virus (TSWV) and tomato yellow leaf curl virus (TYLCV), are major threats to tomato and other vegetable production. Although co-infections of TSWV and TYLCV on the same host plant have been reported on numerous occasions, there is still lack of research attempting to elucidate the mechanisms underlying the relationship between two viruses when they coexist in the same tomato or other plants. After assessing the effect of four TSWV-coded proteins on suppressing TYLCV in TSWV N transgenic Nicotiana benthamiana seedlings, the TSWV N protein proved to be effective in reducing TYLCV quantity and viral symptoms.