[Retracted] Expression of α‑fetoprotein in gastric cancer AGS cells contributes to invasion and metastasis by influencing anoikis sensitivity.

Following the publication of this paper, it was drawn to the Editor's attention by a concerned reader that several instances of duplicated data existed within several of the figures in this article; specifically: i) two pairs of data panels in the four‑panel Fig. 2B (so affecting all the panels in this figure part, which showed the results from migration and invasion assay experiments) were overlapping; ii) a pair of data panels for the fluorescence experiments shown in Fig. 4 were also found to be overlapping; and iii) one set of protein bands in the western blots featured in Fig.

Selective P450 Hydroxylation of the Spiro[3.3]heptane Core as a Route to Potential Drug Fragment Molecules.

Engineered P450 enzyme variants, developed from an initial screening panel of 42 enzymes, convert benzyl spiro[3.3]heptane-2-carboxamide into three distally monohydroxylated regioisomers with essentially complete enantioselectivity. Two α-hydroxyamide derivatives are also produced.

Prediction of potential suitable habitats of Malania oleifera under future climate scenarios based on the MaxEnt model.

Malania oleifera is a nationally Category II protected wild plant in China and a Vulnerable species on the IUCN Red List, specifically distributed in the karst forest, playing a crucial role in maintaining biodiversity and ecological balance in karst fragile ecosystems. In this study, the potential suitable habitats for M. oleifera were simulated by the MaxEnt model using 126 distribution records and 19 environment variables.

Ultrasound enhanced the conjugation of epigallocatechin gallate on whey protein isolate and its influence on the emulsifying property and allergenicity.

Background: The conjugation of polyphenols to proteins provides a method for modifying the structure and properties of proteins.

Methods: This study investigated the roles of ultrasound in the conjugation of epigallocatechin gallate (EGCG) with whey protein isolate (WPI) and its effects on the structural characteristics and properties.

Results And Discussion: The formation of EGCG-WPI conjugates (EW) resulted in a decrease in free amino groups and thiol groups in WPI, accompanied by an increase in size and thermal stability.

Immune Checkpoint Inhibitor-Associated Colitis with Fever as the First Symptom.

Background: In recent years, the application of immunotherapeutic agents in the treatment of non-small cell lung cancer has advanced the current treatment landscape. However, side effects associated with immunotherapy should not be underestimated, as these effects can be life-threatening once they occur. In this article, we report a case of immune checkpoint inhibitor-related colitis presenting with fever as the initial symptom.

Factors related to dropout intention of medical college postgraduates in China: A comparison between students who receive standardized training and non-standardized training.

Introduction: The global shortage of healthcare workers significantly impacts patient well-being and physician workforce development. Ensuring the graduation of every medical student is a critical way to maintain an adequate physician workforce. However, previous studies have shown that dropout intention is very high among medical college postgraduates in China.

Reference intervals for coagulation parameters in chinese adults stratified by sex and age.

Coagulation parameters are critical for the diagnosis and management of bleeding disorders, yet existing reference intervals (RIs) often neglect demographic variations. This study established sex- and age-specific RIs for six coagulation parameters, including lupus anticoagulant (LA) ratio, protein C/S (PC/PS) activity, factor VIII/IX (FVIII/FIX) activity, and von Willebrand factor antigen (vWF Ag), in healthy Chinese adults from Shenzhen and Beijing. Coagulation parameter distributions were explored using kernel density plots and boxplots, with statistical comparisons across sex or age groups.

Clinical features and genetic spectrum of children with primary ciliary dyskinesia in central China: a referral center retrospective analysis.

Background: Despite growing awareness of primary ciliary dyskinesia (PCD) in northern China, few cases have been reported in central China, the most populous region in the country. This study aimed to describe the clinical phenotype and genotype of children with PCD in central China.

Methods: We retrospectively recruited 15 patients with PCD from January 2018 to July 2024.

lncRNA Impaired Male Fertility by Interfering with Sexual Behaviors in Mice.

Long non-coding (lnc) RNAs exhibit tissue-specific expression characteristics and have been shown to be involved in the regulation of various biological processes. The testis is one of the organs with the most abundant lncRNAs. However, the functions of many testis-specific or -enriched lncRNAs in male fertility remain undisclosed.

The Effects of Nitrogen Flow during Carbonization on Enrofloxacin, Methylene Blue, and Phospholipids Adsorption by Silk Sericin-Derived Carbon.

The effect of nitrogen flow during carbonization on bioderived carbon's adsorption performance has not been thoroughly investigated. To fill the gap, the adsorption behavior of silk sericin-based carbon prepared under three different nitrogen flow conditions: (i) without nitrogen flow, (ii) with continuous nitrogen flow, and (iii) with nitrogen flow ceased after heating to 150 °C, were evaluated. In aqueous environments, the CS prepared without nitrogen flow during carbonization exhibited the largest specific surface area and the highest adsorption capacities for methylene blue and enrofloxacin.

Current and Future Landscape of SERCAs' Functions in Non-Excitatory Cells and Diseases.

Calcium (Ca) signaling as the primary intracellular second messenger orchestrates a myriad of physiological processes. Maintaining Ca homeostasis relies on Ca channels, pumps, exchangers, and buffers. Sarco/endoplasmic reticulum Ca-ATPases (SERCAs or ATP2A) encoded by ATP2A1, ATP2A2, or ATP2A3 are primary Ca pumps localized on the endoplasmic reticulum (ER)/sarcoplasmic reticulum (SR) that actively sequester Ca from the cytoplasm back to the ER/SR, thereby preventing the detrimental overload of cytoplasmic Ca concentration.

ST-GTrans: Spatio-temporal graph transformer with road network semantic awareness for traffic flow prediction.

Accurate traffic prediction has significant implications for traffic optimization and management. However, few studies have thoroughly considered the implicit spatial semantic information and intricate temporal patterns. To address these challenges, we propose a spatio-temporal graph transformer with road network semantic awareness (ST-GTrans) for traffic flow prediction, an architecture that extends the transformer to effectively model spatio-temporal dependencies in traffic data.

Electro-Acupuncture Therapy Alleviates Post-Stroke Insomnia by Regulating Sirt1 and the Nrf2-ARE Pathway.

Post-stroke insomnia (PSI) is a common complication following stroke, which seriously affects patients' life quality. Electro-acupuncture (EA) is an innovative form of traditional Chinese acupuncture that combines electricity with needles to achieve the prevention and treatment of diseases. However, there is limited understanding regarding the treatment mechanism of EA in PSI.

Effects of the Loess Plateau on Habitat Quality of the West Qinling Mountains, China.

The West Qinling Mountains is the western extension of the Qinling Mountains, the geographic demarcation line between north and south China. Under the control of the Loess Plateau, the northern part of the West Qinling Mountains has obvious transitional features in terms of topography, climate, soil, and vegetation. In order to explore the effects of the Loess Plateau on habitat quality (HQ) of the West Qinling Mountains, we selected five typical counties with different percentages of the Loess Plateau area based on geomorphic types, climate, water system, vegetation zone, and elevation, and analyzed the spatial and temporal differentiation characteristics of HQ and their influence mechanisms with the help of the InVEST model and geographical detector (GD) model.

Evaluation of Different Drying Methods on the Quality Parameters of Fruits.

(Rupr. and Maxim.; AS) fruit, an underutilized byproduct, possesses significant bioactive potential, yet its quality is highly influenced by drying methods.

Inducing mononuclear cells of patients with CADASIL to construct a CSVD disease model.

Objective: To produce pluripotent stem cells from peripheral blood mononuclear cells (PBMCs) of a patient with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) and culture and differentiate them into vascular organoids, producing a disease model for cerebral small vessel disease (CSVD).

Methods: (1) PMBCs from patients clinically diagnosed with CADASIL (NOTCH3 p.R141C) were induced to differentiate into pluripotent stem cells (iPSCs); the quality and differentiation ability of the iPSCs were determined.

Loss of lncRNAs 1700101O22Rik and 1700027A15Rik causes sperm malformation and subfertility.

Background: The testis is a key reservoir of long non-coding RNAs, yet their physiological roles in male reproduction remain debated. Notably, long non-coding RNAs 1700101O22Rik (22Rik) and 1700027A15Rik (15Rik) are uniquely expressed in the mouse testis. Previous research indicates that both 22Rik and 15Rik play roles in male reproductive processes; however, it is still unclear whether their effects on fertility are cumulative or compensatory.

Molecular pathogenesis of Cryptosporidium and advancements in therapeutic interventions.

Cryptosporidiosis, caused by a Cryptosporidium infection, is a serious gastrointestinal disease commonly leading to diarrhea in humans. This disease poses a particular threat to infants, young children, and those with weakened immune systems. The treatment of cryptosporidiosis is challenging due to the current lack of an effective treatment or vaccine.

Development of competency framework for postgraduate anesthesia training in China: a Delphi study.

Background: Standardized postgraduate medical education (PGME) in anesthesiology is not well-defined in China. Establishing a competency framework for postgraduate anesthesia training (PGAT) is essential for standardizing and enhancing the quality of training and patient care.

Objectives: This study aimed to develop a competency framework for PGAT in China.

Correction: Performance of resistive index and semi-quantitative power doppler ultrasound score in predicting acute kidney injury: A meta-analysis of prospective studies.

[This corrects the article DOI: 10.1371/journal.pone.

Uncovering protein regulation during adventitious root formation in Platycladus orientalis cuttings.

Cell totipotency and pluripotency are the cellular basis for root regeneration in Platycladus orientalis (L.) Franco cuttings, and the regeneration of adventitious roots is a key determinant for improving stem-cutting. However, the cellular basis and physiological regulation of adventitious root formation are still ambiguous.

Examining the mental health trajectories of children and adolescents: a cross-cohort analysis.

Background: Epidemiological samples provide opportunity to understand the development of mental health trajectories to better understand whether such epidemiological data can help to plan and modify service delivery for youth mental health. Variation between countries is not well understood and thus applying evidence from other countries to national strategies limits support service policy and planning. We therefore examine developmental patterns of youth mental health across different countries using the Growing Up in Ireland (GUI) Cohorts, with comparison to existing UK longitudinal cohort data (Millennium Cohort Study, MCS; Growing up in Scotland, GUS).

CABS1 Is Essential for Progressive Motility and the Integrity of Fibrous Sheath in Mouse Epididymal Spermatozoa.

The calcium-binding protein spermatid-associated 1 (CABS1) localizes to the principal piece of mature sperm flagella. Deletion of CABS1 results in subfertility in male mice, possibly due to an impaired annulus in the sperm flagella. However, it is unknown whether there are other mechanisms by which CABS1 affects male fertility.

Histone demethylase JMJD1C advances macrophage foam cell formation and atherosclerosis progression by promoting the transcription of PCSK9.

Macrophage is considered as a critical driving factor in the progression of atherosclerosis (AS), and epigenetic heterogeneity contributes important mechanisms in this process. Here, we identified that a histone demethylase jumonji domain-containing protein 1 C (JMJD1C) is a promising biomarker for atherosclerotic cerebral infarction through clinical analysis. Then, AOPE mice fed with a high fat diet and RAW264.

Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates.

Substrate anchorage is essential for cell migration, and actin polymerization at cell front and myosin contractility at cell rear are known to govern cell forward movement. Yet their differential driving strategies for neutrophil migration on distinct adhesiveness substrates and their contributions to the migration-induced trail formation remain unclear. Here we explore the morphological changes, migration dynamics, and trail formation of neutrophils on ICAM-1 and PLL substrates, with a focus on the relationships among adhesive forces, traction forces, and out-of-plane forces.