Inhibition of aflatoxin B1-induced murine hepatocyte pyroptosis by Bacillus amyloliquefaciens by activation of the Nrf2/HO-1 pathway.

Human and animals are continuously exposed to variable levels of mycotoxin that occur naturally in the diet. Aflatoxin B1 (AFB1) is the most toxic and carcinogenic aflatoxin. Probiotics have a vital role in mycotoxin detoxification.

Structural characterisation of hawthorn polysaccharide and its mechanisms of action against non-alcoholic fatty liver disease.

Non-alcoholic fatty liver disease (NAFLD) represents a significant health risk to humans; however, effective pharmacological treatments are currently unavailable. Hawthorn polysaccharide (HP) possesses a range of beneficial properties that enhance cardiovascular health, diabetes management, antiviral activity, and antioxidant function. However, further investigations are required to clarify the anti-NAFLD effects of HP and elucidate the underlying mechanisms.

PP2A attenuates α-amanitin-induced liver injury by promoting autophagy and inhibiting apoptosis in mouse models.

Poisoning caused by the mushroom toxin α-amanitin accounts for ∼90 % of food poisoning deaths resulting from mushrooms in China. However, Drosophila melanogaster uses PP2A to mitigate effects of α-amanitin. Our objectives were to test the hypothesis that modulation of PP2A protects mammals against deleterious effects of α-amanitin.

Occurrence of antimicrobial resistance and class 1 integrons in isolated from bovine mastitis in China.

Integrons are important genetic elements that allow easy acquisition and dissemination of antimicrobial resistance genes. Studies reporting occurrence of integrons in () isolated from bovine mastitis in large dairy farms across China are scarce. The aim of this study was to investigate the occurrence of class 1 integrons (intI1), antimicrobial resistance (AMR) and associated genes in isolated from bovine mastitis and their associations.

isolated from clinical mastitis cases on large dairy farms in north China: phenotype, genotype of antimicrobial resistance and virulence genes.

() is bovine mastitis pathogen and has thus became a matter of concern to dairy farms worldwide in terms of economic loss. The aims of this study were to (a) determine virulence genes, and (b) characterize the antimicrobial resistance (AMR) profiles and AMR genes and (c) figure out the relationship between AMR phenotypes and genotypes of isolated from dairy cows in north China. A total of 20 virulence genes and 23 AMR genes of 140 isolates collected from 12 farms in six provinces were studied.

Schisandra chinensis inhibits the entry of BoHV-1 by blocking PI3K-Akt pathway and enhances the m6A methylation of gD to inhibit the entry of progeny virus.

Schisandra chinensis, a traditional Chinese medicine known for its antitussive and sedative effects, has shown promise in preventing various viral infections. Bovine herpesvirus-1 (BoHV-1) is an enveloped DNA virus that causes respiratory disease in cattle, leading to significant economic losses in the industry. Because the lack of previous reports on Schisandra chinensis resisting BoHV-1 infection, this study aimed to investigate the specific mechanisms involved.

Salidroside Inhibits α-Amanitin-Induced AML-12 Cell Apoptosis via the Regulation of PINK1/Parkin-Mediated Mitophagy and Mitochondrial Function.

Poisoning caused by the mushroom , due to the toxin α-amanitin, accounts for approximately 90% of food poisoning deaths in China with no specific antidotes. To investigate the role of salidroside (Sal) in α-amanitin (α-AMA)-induced mitophagy, mouse liver cells AML-12 were exposed to α-AMA in the presence of Sal or not. Intracellular reactive oxygen species (ROS) levels were measured using a ROS detection kit, mitochondrial activity was evaluated using a mitochondrial red fluorescent probe kit or JC-1 dye, and protein expression levels of PINK1, Parkin, LC3 II, P62, Bax, Bcl-2, Caspase 3, Cleaved-Caspase 3, PARP I, and Cleaved-PARP I were detected through Western blot.

Antioxidative Sirt1 and the Keap1-Nrf2 Signaling Pathway Impair Inflammation and Positively Regulate Autophagy in Murine Mammary Epithelial Cells or Mammary Glands Infected with .

mastitis in cattle infects mammary epithelial cells. Although oxidative responses often remove intracellular microbes, survives, but the mechanisms are not well understood. Herein, we aimed to elucidate antioxidative mechanisms during pathogenesis of after isolation from clinical bovine mastitis milk samples.

Quercetin protects cadmium-induced renal injuries in mice by inhibiting cell pyroptosis.

The toxic heavy metal cadmium (Cd) has a significant impact on kidney health. Documents manifested that non-toxic flavonoid quercetin can reduce Cd-induced kidney damage by reducing oxidative stress and inhibiting apoptosis, while the effect of quercetin on Cd-induced renal cell pyroptosis has not been elucidated. In this study, we established a model of Cd poisoning treated with quercetin both and .

Fungi, including Pithomyces chartarum cause facial eczema and inflammation in grazing sheep in Western China.

Facial eczema is often found in flocks of grazing sheep in China. To investigate fungi species those cause disease and pathological roles. Forage and soil samples were collected during the pathogenic season and cultured.

The prevalence of coagulase-negative staphylococcus associated with bovine mastitis in China and its antimicrobial resistance rate: a meta-analysis.

To contribute to the treatment decision and optimize coagulase-negative staphylococcus (CNS) control programs, we conducted a meta-analysis to investigate the epidemiology and antimicrobial resistance rates of coagulase-negative staphylococcus associated with bovine mastitis in China. Three databases (PubMed, Google scholar and China National Knowledge Infrastructure database) were utilized to obtain relevant publications. A total of 18 publications were included in our research, and 3 of them included antimicrobial resistant (AMR) test.

The prevalence of bovine mastitis-associated in China and its antimicrobial resistance rate: A meta-analysis.

In this study, to optimize the control program, a meta-analysis was conducted to investigate the epidemiology and antimicrobial resistance (AMR) profile of -associated bovine mastitis in China from 2000 to 2020. A total of 33 publications from PubMed, Google Scholar, and China National Knowledge Infrastructure (CNKI) database were included in our research, among which nine publications included the AMR test. The pooled prevalence of was 36.

The Prevalence of spp. Associated With Bovine Mastitis in China and Its Antimicrobial Resistance Rate: A Meta-Analysis.

Understanding distribution of bovine mastitis pathogen spp. can contribute to the treatment decision and the control within programs of bovine mastitis, we conducted a meta-analysis to investigate the epidemiology and antimicrobial resistance rates of spp. associated with bovine mastitis in China.

The prevalence, molecular characterization and antimicrobial resistance profiling of isolated from clinical mastitis cases on large dairy farms in China.

This research communication aims to characterize the prevalence, molecular characterization and antimicrobial resistance profiling of Streptococcus agalactiae isolated from clinical mastitis in China. A total of 140 Strep. agalactiae isolates were identified from 12 out of 201 farms in 6 provinces, overall herd prevalence was 18.

Biological Characteristics and Pathogenicity of Isolated From Clinical Bovine Mastitis in a Chinese Dairy Herd.

() was first reported in ovine subclinical mastitis milk and post-mortem examination organs in Spain and the United Kingdom in 1999; subsequently, it appeared in cattle, horse, goat, and human. However, isolation and characterization of the pathogen from clinical bovine mastitis is unknown. The objective of this study was to identify the pathogen in clinical bovine mastitis.

Autophagy Promotes α-Amanitin-Induced Apoptosis of Hepa1-6 Liver Cells.

It is estimated that 90% of deaths from food poisoning in China can be attributed to poisoning, whose main toxin is α-amanitin. Studies showed that apoptosis plays a critical role in liver injuries induced by α-amanitin. Although the relationship between autophagy and apoptosis in different liver models has been addressed many times, whether autophagy plays a pro or con effect on α-amanitin-induced apoptosis has not been clarified.

Mycoplasma bovis subverts autophagy to promote intracellular replication in bovine mammary epithelial cells cultured in vitro.

Mycoplasma species are the smallest prokaryotes capable of self-replication. To investigate Mycoplasma induced autophagy in mammalian cells, Mycoplasma bovis (M. bovis) and bovine mammary epithelial cells (bMEC) were used in an in vitro infection model.

Facile hydrothermal synthesis of TiCT-TiO nanocomposites for gaseous volatile organic compounds detection at room temperature.

The gaseous volatile organic compounds (VOCs) sensors with high-selectivity and low-power consumption have been expected for practical applications in environmental monitoring and disease diagnosis. Herein, we demonstrate a room-temperature VOCs gas sensor with enhanced performance based on TiCT-TiO nanocomposites. The TiCT-TiO nanocomposites with regular morphology are successfully synthesized via a facile one-step hydrothermal synthesis strategy by using TiCT itself as titanium source.

Association of NOS2A gene polymorphisms with susceptibility to bovine tuberculosis in Chinese Holstein cattle.

Bovine tuberculosis (bTB) is a global zoonotic disease that has detrimental economic impacts worldwide. The NOS2A gene plays a key role in immunological control of many infectious diseases. However, research on the association between NOS2A polymorphisms and bTB infection in Holstein cattle reared on the Yunnan-Guizhou plateau of China is scarce.

Enhanced ammonia sensing performance based on MXene-TiCT multilayer nanoflakes functionalized by tungsten trioxide nanoparticles.

Low-power consumption and high sensitivity are highly desirable for a vast range of NH sensing applications. As a new type of two-dimension (2D) material, TiCT is extensively studied for room temperature NH sensors recently. However, the TiCT MXene based gas sensors suffer mainly from low sensitivity.

Characterization of Streptococcus lutetiensis isolated from clinical mastitis of dairy cows.

Streptococcus lutetiensis, previously termed Streptococcus bovis type II/1, has rarely been associated with bovine mastitis. The objectives of this work were to characterize the molecular diversity, antimicrobial resistance profiles, virulence genes of Strep. lutetiensis (n = 37) isolated from bovine clinical mastitis, as well as its pathogenic effects in a murine mastitis model.

Mycoplasma bovis-generated reactive oxygen species and induced apoptosis in bovine mammary epithelial cell cultures.

Mycoplasma bovis is an important cause of bovine mastitis in China and worldwide. We hypothesized that M. bovis damages bovine mammary epithelial cells (bMEC), with the degree of damage varying among field isolates.

Antimicrobial resistance profiles of 5 common bovine mastitis pathogens in large Chinese dairy herds.

The prevalence of antimicrobial resistance (AMR) is increasing in human and animal pathogens, becoming a concern worldwide. However, prevalence and characteristics of AMR of bovine mastitis pathogens in large Chinese dairy herds are still unclear. Therefore, our objective was to determine the AMR profile of bacteria isolated from clinical mastitis in large (>500 cows) Chinese dairy herds.

The Characteristics of Staphylococcus aureus Small Colony Variant Isolated from Chronic Mastitis at a Dairy Farm in Yunnan Province, China.

Staphylococcus aureus is a major causative agent leading to bovine mastitis and has specific phonotypical characteristics including small colony, slow growth, and decreased hemolysis, therefore named as the small colony variants (SCVs). Out of 30 tested samples of the chronic S. aureus cases, one strain of SCVs (S.