Mechanistic Insights into Aflatoxin B and M Degradation by HNGD-Mq02 from Wheat Koji and Its Application in Food Detoxification.

Aflatoxin B (AFB), the most toxic and prevalent mycotoxin in food and feed, and its hydroxylated metabolite aflatoxin M (AFM), common in dairy products, pose serious health risks. Effective degradation of these toxins is thus a key goal in food safety. In this study, HNGD-Mq02 was isolated and assessed for its ability to degrade AFB and AFM.

High-Performance Polyimide Design: Machine Learning-Accelerated Multiobjective Optimization.

Polyimides (PIs) with superior transparency, mechanical robustness, and thermal stability are essential for advanced applications such as flexible electronics, foldable displays, and aerospace components, but simultaneous optimization of these properties remains challenging. Here, we present a data-driven multiobjective optimization framework for systematic polyimide design, supported by high-quality data sets extracted with the assistance of large language models. Leveraging robust machine learning models validated by the experimental synthesis and evaluation of five PI films, our method precisely predicts material performance and effectively deciphers complex structure-property relationships influenced by charge transfer complexes.

Analysis of dynamic contrast-enhanced T1-weighted imaging parameters in type II TIC breast lesions.

Background: In dynamic contrast-enhanced T1-weighted imaging (DCE-T1WI) of breast lesions, type III time-intensity curves (TICs) are associated with malignant lesions, and type I TICs are associated with benign lesions, but the association of type II curves with the status of breast lesions remains controversial. This study aimed to analyze the semi-quantitative parameters derived from DCE-T1WI in patients with type II TIC breast lesions and to develop a nomogram for the benign/malignant classification of lesions with type II TICs.

Methods: Data of patients with type II TIC breast lesions were retrospectively collected.

The Magnaporthe oryzae effector Pwl2 alters HIPP43 localization to suppress host immunity.

The rice blast fungus Magnaporthe oryzae secretes a battery of effector proteins to facilitate host infection. Among these effectors, pathogenicity toward weeping lovegrass 2 (Pwl2) was originally identified as a host specificity determinant for the infection of weeping lovegrass (Eragrostis curvula) and is also recognized by the barley (Hordeum vulgare) Mla3 resistance protein. However, the biological activity of Pwl2 remains unknown.

Response of earthworm enzyme activity and gut microbial functional diversity to carbendazim in the manured soil.

The effect of pesticide pollution on environmental microorganisms in soil has become the focus of widespread concern in society today. The response of earthworm gut and surrounding soil microbial functional diversity and enzyme activity to carbendazim (CBD) was studied in a soil-earthworm ecosystem amended with manure. In the experiment, CBD was added to the manured soil (MS).

Characterization, Mechanism, and Application of Dipeptidyl Peptidase III: An Aflatoxin B-Degrading Enzyme from HNGD-TM15.

Aflatoxin B is a notorious mycotoxin with mutagenicity and carcinogenicity, posing a serious hazard to human and animal health. In this study, an AFB-degrading dipeptidyl-peptidase III mining from HNGD-TM15 (ADPP III) with a molecular weight of 79 kDa was identified. ADPP III exhibited optimal activity toward AFB at 40 °C and pH 7.

Thermo-Alkali-Tolerant Recombinant Laccase from and Its Degradation Potential against Zearalenone and Aflatoxin B.

The enzymatic biodegradation of mycotoxins in food and feed has attracted the most interest in recent years. In this paper, the laccase gene from was cloned and expressed in BL 21(D3). The sequence analysis indicated that the gene consisted of 1533 bp.

The phosphorylation landscape of infection-related development by the rice blast fungus.

Many of the world's most devastating crop diseases are caused by fungal pathogens that elaborate specialized infection structures to invade plant tissue. Here, we present a quantitative mass-spectrometry-based phosphoproteomic analysis of infection-related development by the rice blast fungus Magnaporthe oryzae, which threatens global food security. We mapped 8,005 phosphosites on 2,062 fungal proteins following germination on a hydrophobic surface, revealing major re-wiring of phosphorylation-based signaling cascades during appressorium development.

Characterization, Structural Analysis, and Thermal Stability Mutation of a New Zearalenone-Degrading Enzyme Mined from .

Zearalenone (ZEN) is a widespread mycotoxin that causes serious damage to animal husbandry and poses a threat to human health. A screen of ZEN-degrading soil bacteria yielded YT-4, which yielded 80% ZEN degradation after 6 h and 95% after 36 h. The gene sequence encoding the degradative enzyme ZENY was mined from the genome of YT-4 and expressed in yeast.

Mechanism of inactivation of Aspergillus flavus spores by dielectric barrier discharge plasma.

Dielectric barrier discharge plasma (DBDP) displays strong against fungal spores, while its precise mechanism of spore inactivation remains inadequately understood. In this study, we applied morphological, in vivo and in vitro experiments, transcriptomics, and physicochemical detection to unveil the potential molecular pathways underlying the inactivation of Aspergillus flavus spores by DBDP. Our findings suggested that mycelium growth was inhibited as observed by SEM after 30 s treatment at 70 kV, meanwhile spore germination ceased and clustering occurred.

Thiamethoxam dynamics in pepper plants: Deciphering deposition and dissipation pattern across diverse planting modes and regions.

To reduce the application dosage of thiamethoxam (TMX), we investigated the deposition and dissipation patterns in a pepper-planted ecosystem under different planting modes across four regions in China, namely Hainan (HN), Zhejiang (ZJ), Anhui (AH) and Hebei (HB). This study focused on the deposition and dissipation of TMX at concentrations of 63.00, 47.

Mining Lactonase Gene from Aflatoxin B-Degrading Strain and Degrading Properties of the Recombinant Enzyme.

Mycotoxins are toxic secondary metabolites mainly produced by filamentous fungal species that commonly contaminate food and feed. Aflatoxin B (AFB) is extremely toxic and seriously threatens the health of humans and animals. In this work, the HNGD-A6 was obtained and showed a 94.

A molecular mechanosensor for real-time visualization of appressorium membrane tension in Magnaporthe oryzae.

The rice blast fungus Magnaporthe oryzae uses a pressurized infection cell called an appressorium to drive a rigid penetration peg through the leaf cuticle. The vast internal pressure of an appressorium is very challenging to investigate, leaving our understanding of the cellular mechanics of plant infection incomplete. Here, using fluorescence lifetime imaging of a membrane-targeting molecular mechanoprobe, we quantify changes in membrane tension in M.

Correction: Genomic surveillance uncovers a pandemic clonal lineage of the wheat blast fungus.

[This corrects the article DOI: 10.1371/journal.pbio.

Development and validation of a nomogram for postoperative sleep disturbance in adults: a prospective survey of 640 patients undergoing spinal surgery.

Background: Postoperative sleep disturbance (PSD) is a prevalent clinical complication that may arise due to various factors. The purpose of this investigation is to identify the risk factors for PSD in spinal surgery and establish a risk prediction nomogram.

Methods: The clinical records of individuals who underwent spinal surgery from January 2020 to January 2021 were gathered prospectively.

Successful mechanical thrombectomy in acute bilateral M1 middle cerebral artery occlusion: a case report and literature review.

Background: Acute bilateral occlusion of the middle cerebral artery (MCA) is a very rare condition, and most cases are accompanied by a poor prognosis. However, mechanical thrombectomy (MT) for bilateral MCA is challenging. Here, we report a case of acute unilateral MCA occlusion with sequential acute occlusion of the bilateral MCA during intravenous thrombolysis (IVT).

The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors.

The rice blast fungus Magnaporthe oryzae causes a devastating disease that threatens global rice (Oryza sativa) production. Despite intense study, the biology of plant tissue invasion during blast disease remains poorly understood. Here we report a high-resolution transcriptional profiling study of the entire plant-associated development of the blast fungus.

Research progress of ochratoxin a bio-detoxification.

Ochratoxins (OTs) is an extremely toxic mycotoxin in which Ochratoxin A (OTA) is the most toxic and prevalent in the ochratoxin family. OTA is among the five most critical mycotoxins that are subject to legal regulations. Animals and humans may be exposed to OTA through dietary intake, inhalation, and dermal contact.

Using drug-coated balloons for symptomatic vertebral artery origin stenosis: A systematic review and meta-Analysis.

Background: Endovascular stenting has emerged as an effective treatment for patients with symptomatic vertebral artery origin stenosis (VAOS), but the incidence of severe restenosis is concerning. Angioplasty alone with a drug-coated balloon (DCB) is a potential treatment for VAOS. The purpose of this systematic review and meta-analysis was to assess the utility of DCB angioplasty for VAOS.

Tracing and quantifying the source of heavy metals in agricultural soils in a coal gangue stacking area: Insights from isotope fingerprints and receptor models.

Historic coal gangue stacking probably brings heavy metals (HMs) into the surrounding agricultural soil, posing potential harm to human and environmental health. For better controlling and preventing agricultural soil HMs pollution, the screening of priority pollutants and identification of their pollution pathways are urgent in coal gangue stacking areas. Thus, this study selected a coal gangue stacking area in Chongqing, China as the research object and conducted the pollution evaluation, spatial distribution and source apportionment of the HMs (Cd, Cr, Ni, Cu, Zn, As, Pb and Hg) in surrounding agricultural soil.