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Article Abstract
Mycotoxins are toxic secondary metabolites mainly produced by filamentous fungal species that commonly contaminate food and feed. Aflatoxin B (AFB) is extremely toxic and seriously threatens the health of humans and animals. In this work, the HNGD-A6 was obtained and showed a 94.66% removal ability of AFB by employing extracellular enzymes as the degrading active substance. The degradation products were P1 (AFD, CHO) and P2 (CHNO), and their toxicity was greatly reduced compared to that of AFB. The AttM gene was mined by BlastP comparison and successfully expressed in BL21. AttM could degrade 86.78% of AFB at pH 8.5 and 80 °C, as well as 81.32% of ochratoxin A and 67.82% of zearalenone. The ability of AttM to degrade a wide range of toxins and its resistance to high temperatures offer the possibility of its use in food or feed applications.
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| http://dx.doi.org/10.1021/acs.jafc.3c05725 | DOI Listing |
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