Microvesicle-transferred mitochondria trigger cGAS-STING and reprogram metabolism of macrophages in sepsis.

The inflammatory cytokine storm is a hallmark of sepsis and is highly correlated with organ injury. Therefore, inhibiting inflammatory cytokine production is a straightforward strategy for effectively treating this disease. In this study, we found that microvesicles from lipopolysaccharide (LPS)-primed macrophages could transfer mitochondria to other macrophages and alter their biological functions.

Tumor-targeted nano-delivery system of camptothecin nanocrystals for the effective treatment of triple negative breast cancer.

Clinical studies have shown that camptothecin and its derivatives have good antitumor activity for triple negative breast cancer (TNBC). However, the poor water solubility and low therapeutic selectivity limited their use in the clinic. Drug delivery systems are of great significance in enhancing drug solubility and precisely controlling drug release, thus increasing the therapeutic efficacy and reducing the toxicity.

From operating room to recovery: Evidence and gaps in cardiac surgical nursing integrative psychological support.

This review explored the application and effectiveness of and research gaps in integrative psychological support in patients undergoing cardiac surgery during the perioperative period. These patients often encounter psychological issues such as anxiety and depression, which affect their postoperative recovery and quality of life. Previous research indicated that preoperative psychological assessments and interventions significantly enhance patients' psychological states and postoperative outcomes.

Two DNA-binding One Zinc Finger transcription factors, MdCDOF3 and MdDOF3.6, accelerate leaf senescence by activating in response to sorbitol signaling in apple.

Leaf senescence, an essential component of the plant life cycle, seriously affects the productivity of numerous commercial crops, with cytokinins serving as crucial regulators in delaying this process. Here, we observed that apple () leaves exhibiting deficiencies in sorbitol synthesis due to antisense inhibition of () presented an increase in cytokinin content and exhibited a delay in leaf senescence, in contrast to wild-type (WT) leaves. Transcriptome analysis indicated that the expression of (), encoding a key enzyme in the cytokinin degradation pathway, was significantly downregulated in the antisense lines.

Fluorescence Visual Identification of Four Main Varieties of Tibetan Medicinal Berberis cortex Based on Site-Specific PCR Technology.

Background: Berberis cortex is a typical multi-origin species in Tibetan medicine, with varying medicinal effects among different species. Establishing a rapid and accurate identification method for the major species of Tibetan medicinal B. cortex, including Berberis vernae Schneid.

Using proteomics and single-cell sequencing to analyze the pathogenesis of recurrent implantation failure associated with uterine natural killer cells.

Problem: Recurrent implantation failure (RIF) affects about 10% of infertility patients and may involve mid-luteal phase endometrial natural killer (NK) cells. The pathogenesis of NK cells in RIF remains unclear.

Method Of Study: Our study integrated proteomics data from endometrial tissues of six RIF patients and six controls, with single-cell sequencing insights.

Dynamic CtDNA monitoring in adjuvant therapy and recurrence for colorectal liver metastases (PKUCRLM-01): a prospective study.

Introduction: Effective prediction methods for monitoring the risk of recurrence of colorectal cancer liver metastasis (CRLM) and guiding postoperative adjuvant treatment (ACT) are currently lacking. This study aimed to evaluate the value of postoperative dynamic circulating tumor DNA (ctDNA) monitoring in guiding ACT and predicting recurrence compared with other clinicopathological factors.

Methods: This prospective study enrolled 414 consecutive patients with CRLM who underwent radical resection.

Predictive Factors for Chemotherapy Response in Colorectal Liver Metastasis: A Retrospective Study Utilizing Next-Generation Sequencing.

Background: This study aimed to identify predictive factors for chemotherapy response in colorectal liver metastasis (CRLM) patients.

Methods: Eligible participants with CRLM who had undergone at least two systemic chemotherapy cycles postdiagnosis were retrospectively analyzed. They were categorized as responders and nonresponders based on tumor size reduction.

Enrichment of two important metabolites D-galacturonic acid and D-glucuronic acid inhibits MdHb1-mediated fruit softening in apple.

In apples, fruit firmness is a crucial quality trait influencing fruit storability, transportability, shelf life and consumer preference. However, the genetic network underlying this trait remains unclear. Therefore, the present study investigated the changes in apple fruit at different stages of postharvest storage using a combination of transcriptomic and metabolomic analyses.

[Advances in research on gender differences in autism spectrum disorders].

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social impairments, repetitive behaviors, and restricted interests. Studies have shown that it is more prevalent in males than females. Although this issue has attracted academic attention since the 20th century, the specific mechanisms underlying the gender differences in ASD remain unclear.

Genome-wide identification and unveiling the role of MAP kinase cascade genes involved in sugarcane response to abiotic stressors.

Background: The MAP Kinase cascade system is a conserved signaling mechanism essential for plant development, growth, and stress tolerance. Thus far, genes from the MAPK cascade have been identified in several plant species but remain uncharacterized in the polyploid Saccharum spp. Hybrid R570 genome.

[Two cases of creatine deficiency syndrome caused by gene mutations and literature review].

Objectives: To summarize the clinical manifestations and genetic characteristics of creatine deficiency syndrome (CDS) caused by gene mutations.

Methods: A retrospective analysis was conducted on the clinical and genetic data of two children diagnosed with GAMT deficiency-type CDS at the Children's Medical Center of Xiangya Hospital, Central South University, from December 2020 to December 2024.

Results: The two patients presented with symptoms in infancy, and both had compound heterozygous mutations in the gene.

gen. et sp. nov., a New Cup Moth from Southern China Based on Morphological and Molecular Analysis (Lepidoptera: Zygaenoidea: Limacodidae).

A new genus and species of Limacodidae, gen. et sp. nov.

A linker histone acts as a transcription factor to orchestrate malic acid accumulation in apple in response to sorbitol.

High carbohydrate availability promotes malic acid accumulation in fleshy fruits, but the underlying mechanism is not known. Here, we show that antisense repression of ALDOSE-6-PHOSPHATE REDUCTASE in apple (Malus domestica) decreases the concentrations of sorbitol and malate and the transcript levels of several genes involved in vacuolar malate transport, including the aluminum-activated malate transporter (ALMT) gene MdALMT9 (Ma1), the P-ATPase gene MdPH5, the MYB transcription factor gene MdMYB73, and the cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1, in fruit and leaves. We identified a linker histone H1 variant, MdH1.

Global research on oral cancer: A bibliometric analysis based on 82 highly cited publications from 2014 to 2024.

Objective: Oral cancer refers to a group of malignancies. The disease's complexity requires a multidisciplinary approach, encompassing oncology, dentistry, epidemiology, molecular biology, and other fields. Given this multifaceted nature, bibliometrics has emerged as a crucial tool to navigate the vast array of academic literature surrounding oral cancer.

High-precision identification of highly similar Pinelliae Rhizoma and adulterated Rhizoma pinelliae pedatisectae through deep neural networks based on vision transformers.

Pinelliae Rhizoma is a key ingredient in botanical supplements and is often adulterated by Rhizoma Pinelliae Pedatisectae, which is similar in appearance but less expensive. Accurate identification of these materials is crucial for both scientific and commercial purposes. Traditional morphological identification relies heavily on expert experience and is subjective, while chemical analysis and molecular biological identification are typically time consuming and labor intensive.

Cellular plasticity and fate determination in gastric carcinogenesis.

Cellular plasticity serves as a crucial biological phenomenon in humans, integral to tissue repair and maintenance of dynamic environmental homeostasis post-injury. However, dysregulated activation of this beneficial mechanism can pave the way for tumorigenesis and cancer progression. In this review, we synthesize recent advancements concerning the properties and roles of gastric epithelial cells, with a special emphasis on cellular plasticity and fate specification during the progress of gastric tumorigenesis.

Total flavonoids of Astragalus protects glomerular filtration barrier in diabetic kidney disease.

Background: Diabetic kidney disease (DKD) is a prevalent complication of diabetes and the leading cause of end-stage renal disease. Recent evidence suggests that total flavonoids of Astragalus (TFA) has promising effects on diabetes; however, its influence on DKD and the underlying mechanism remains unclear.

Methods: In this study, we induced the DKD model using streptozotocin (STZ) in male C57BL/6J mice and utilized glomerular endothelial cell (GEC) lines for in vitro investigations.

Multi-Omics Research Accelerates the Clarification of the Formation Mechanism and the Influence of Leaf Color Variation in Tea () Plants.

Tea is a popular beverage with characteristic functional and flavor qualities, known to be rich in bioactive metabolites such as tea polyphenols and theanine. Recently, tea varieties with variations in leaf color have been widely used in agriculture production due to their potential advantages in terms of tea quality. Numerous studies have used genome, transcriptome, metabolome, proteome, and lipidome methods to uncover the causes of leaf color variations and investigate their impacts on the accumulation of crucial bioactive metabolites in tea plants.

Two new species and a new record of the genus Cabardites Volynkin & ern (Lepidoptera, Erebidae, Arctiinae) from China.

Two new species of the genus Cabardites Volynkin & ern, 2021, C. xinyui sp. nov.

[Protective effect of Liujing Toutong Tablets on rats with permanent cerebral ischemia via NF-κB signaling pathway].

This study investigated the neuroprotective effects and underlying mechanism of Liujing Toutong Tablets(LJTT) on a rat model of permanent middle cerebral artery occlusion(pMCAO). The pMCAO model was established using the suture method. Eighty-four male SPF-grade SD rats were randomly divided into a sham operation group, a model group, a nimodipine group(0.

[Prediction analysis of quality markers and resource evaluation of Artemisiae Argyi Folium based on chemical composition and network pharmacology].

This study is based on ultra-high-performance liquid chromatography(UPLC), gas chromatography-mass spectrometry(GC-MS), and network pharmacology methods to analyze and predict potential quality markers(Q-markers) of Artemisiae Argyi Folium. First, UPLC and GC-MS techniques were used to analyze the content of 12 non-volatile components and 8 volatile components in the leaves of 33 Artemisia argyi germplasm resources as candidate Q-markers. Subsequently, network pharmacology was employed to construct a "component-target-pathway-efficacy" network to screen out core Q-markers, and the biological activity of the markers was validated using molecular docking.

A segmentation model to detect cevical lesions based on machine learning of colposcopic images.

Background: Semantic segmentation is crucial in medical image diagnosis. Traditional deep convolutional neural networks excel in image classification and object detection but fall short in segmentation tasks. Enhancing the accuracy and efficiency of detecting high-level cervical lesions and invasive cancer poses a primary challenge in segmentation model development.

Association of soluble suppression of tumorigenicity 2 protein with new-onset atrial fibrillation in patients with acute ST-segment elevation myocardial infarction undergoing primary PCI.

Background: Previous studies have indicated that the soluble suppression of tumorigenicity 2 protein (sST2) is associated with new-onset atrial fibrillation (NOAF) in patients diagnosed with coronary artery disease (CAD). However, the predictive value of sST2 in patients with acute ST-segment elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI) has not been well studied.

Methods: A total of 580 patients with STEMI undergoing primary PCI were consecutively recruited between January 2021 and January 2023.