High-Level Production of Abscisic Acid by Multi-Strategy Combination Optimization in Botrytis cinerea.

Abscisic acid (ABA), a crucial phytohormone, is industrially synthesized using Botrytis cinerea and has wide-ranging applications in agriculture, forestry, and environmental conservation. However, the industrial fermentation of ABA using B. cinerea still faces numerous challenges.

Recent advances and multiple strategies for the synthesis of terpenoid fragrances and flavors in model microorganisms.

Terpenoids, known for their structural and functional diversity, play a significant role in various fields such as energy, cosmetics, pharmaceuticals, and fragrances. With the increasing globalization of the fragrance and flavor market, there is an urgent need to develop microbial cell factories to sustainably produce terpenoids through fermentation using renewable raw materials. With advancements in biotechnology, constructing efficient microbial cell factories for the heterologous synthesis of terpenoids to meet production demands has become feasible.

Abscisic Acid and Its Producer : Recent Advances and Future Perspectives.

Abscisic acid (ABA), a pivotal plant growth regulator, is extensively utilized in agricultural production, ecological vegetation restoration, and landscape greening. Although remains the primary strain for industrial ABA production, its suboptimal efficiency limits large-scale applications. Recent advancements have elucidated the biosynthetic pathway of ABA.

A Genetic Algorithms-Based Neural Network Model to Monitor Gibberellic Acid GA3 Fermentation Process by Fusarium fujikuroi.

A genetic algorithm-optimized neural network (ANN-GA) was developed for real-time monitoring of gibberellin (GA3) production during Fusarium fujikuroi fermentation. This model addresses the limitations of traditional off-line detection methods, such as contamination risks and delayed feedback, by integrating six critical inputs-initial glucose concentration, fermentation time, temperature, pH, dissolved oxygen, and rotational speed-to predict glucose consumption and GA3 synthesis with an accuracy of 99.41%.

Transcriptome-Based Mining of the Strong Promoters for Hyperproduction of Gibberellin GA3 in .

Gibberellin GA3 is a plant growth regulator with significant applications in agriculture, and has gained attention as an excellent host for the industrial production of GA3. Although numerous gene-editing tools have been developed, the precise metabolic flux regulation in was significantly hindered because the endogenous promoters were rarely identified. In this study, a library containing 20 potential promoters was mined and constructed for the first time through transcriptome sequencing.

Recent advances in the application of CRISPR/Cas-based gene editing technology in Filamentous Fungi.

Filamentous fungi are essential industrial microorganisms that can serve as sources of enzymes, organic acids, terpenoids, and other bioactive compounds with significant applications in food, medicine, and agriculture. However, the underdevelopment of gene editing tools limits the full exploitation of filamentous fungi, which still present numerous untapped potential applications. In recent years, the CRISPR/Cas (clustered regularly interspaced short palindromic repeats) system, a versatile genome-editing tool, has advanced significantly and been widely applied in filamentous fungi, showcasing considerable research potential.

Enhancing abscisic acid production in through metabolic engineering based on a constitutive promoter library.

Abscisic acid (ABA) is an important plant growth regulator with broad applications in agriculture, forestry, and other fields. Currently, the industrial production of ABA primarily relies on microbial fermentation using , but its genetic toolbox is limited. To address this, we first screened 10 strong constitutive promoters from the genome of through transcriptomic analysis.

A comprehensive review on the application of neural network model in microbial fermentation.

The development of high-performance strains and the continuous breakthrough of strain screening technology also pose challenges to downstream fermentation optimization and scale-up. Therefore, neural network models are utilized to optimize the fermentation process to meet the goals of boosting yield or lowering cost, with the use of artificial intelligence technology in conjunction with the peculiarities of the fermentation process. High-performance strains' yield rise and fermentation process amplification will be sped up with the aid of neural network models.

Advances in Flavonoid and Derivative Biosynthesis: Systematic Strategies for the Construction of Yeast Cell Factories.

Flavonoids, a significant group of natural polyphenolic compounds, possess a broad spectrum of pharmacological effects. Recent advances in the systematic metabolic engineering of yeast cell factories (YCFs) provide new opportunities for enhanced flavonoid production. Herein, we outline the latest research progress on typical flavonoid products in YCFs.

A comprehensive review on the recent advances for 5-aminolevulinic acid production by the engineered bacteria.

5-Aminolevulinic acid (5-ALA) is a non-proteinogenic amino acid essential for synthesizing tetrapyrrole compounds, including heme, chlorophyll, cytochrome, and vitamin B12. As a plant growth regulator, 5-ALA is extensively used in agriculture to enhance crop yield and quality. The complexity and low yield of chemical synthesis methods have led to significant interest in the microbial synthesis of 5-ALA.

Establishment of a selectable marker recycling system for iterative gene editing in .

Gibberellic acid (GA3) is a vital plant growth hormone widely used in agriculture. Currently, GA3 production relies on liquid fermentation by the filamentous fungus . However, the lack of an effective selection marker recycling system hampers the application of metabolic engineering technology in , as multiple-gene editing and positive-strain screening still rely on a limited number of antibiotics.

Improving Gibberellin GA Production with the Construction of a Genome-Scale Metabolic Model of .

Liquid fermentation is the primary method for GA production using. However, production capacity is limited due to unknown metabolic pathways. To address this, we constructed a genome-scale metabolic model (CY1235) with 1753 reactions, 1979 metabolites, and 1235 genes to understand the GA regulation mechanisms.

High-Level Production of Patchoulol in via Systematic Engineering Strategies.

Patchoulol is an important sesquiterpene alcohol with a strong and lasting odor, which has led to prominent applications in perfumes and cosmetics. In this study, systematic metabolic engineering strategies were adopted to create an efficient yeast cell factory for patchoulol overproduction. First, a baseline strain was constructed by selecting a highly active patchoulol synthase.

High-yield α-humulene production in Yarrowia lipolytica from waste cooking oil based on transcriptome analysis and metabolic engineering.

Background: α-Humulene is an important biologically active sesquiterpene, whose heterologous production in microorganisms is a promising alternative biotechnological process to plant extraction and chemical synthesis. In addition, the reduction of production expenses is also an extremely critical factor in the sustainable and industrial production of α-humulene. In order to meet the requirements of industrialization, finding renewable substitute feedstocks such as low cost or waste substrates for terpenoids production remains an area of active research.

RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi.

Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with a wide market share. Due to intrinsic advantages, liquid fermentation of Fusarium fujikuroi has become the sole method for industrial GA3 production, but the broader application of GA3 is hindered by low titer. In this study, we combined atmospheric and room-temperature plasma (ARTP) with ketoconazole-based screening to obtain the mutant strain 3-6-1 with high yield of GA3.