Implementing Undergraduate Medical Education Reform to Enhance Problem-Solving and Practical Skills.

Background: Undergraduate medical education in China faces significant gaps in integrating theory with practice, with 32.7% of graduates requiring remedial procedural training.

Objective: To implement and evaluate a competency-based reform targeting problem-solving and practical skills.

PD-L1/ITGB4 Axis Modulates Sensitivity of Hepatocellular Carcinoma to Sorafenib via FAK/AKT/mTOR Signaling Pathway.

Background: Hepatocellular carcinoma (HCC) frequently develops resistance to sorafenib, a first-line treatment for advanced HCC. While PD-L1 contributes to immune evasion and direct tumor survival, its role in modulating sorafenib resistance via non-immunological pathways remains unclear. This study investigates the PD-L1/ITGB4 axis in regulating sorafenib sensitivity.

Rare Primary Diffuse Large B-Cell Lymphoma Confined to Bone Marrow: Features and Prognosis.

Background: Primary bone marrow diffuse large B-cell lymphoma (PBM-DLBCL) represents an uncommon yet clinically aggressive hematologic malignancy. Despite its significant clinical impact, this entity lacks standardized diagnostic criteria in current WHO classifications.

Methods: We performed a retrospective analysis of 55 PBM-DLBCL cases from our institutional database and published literature (2001-2022) to characterize disease features and identify prognostic factors, with particular focus on assessing how different treatment regimens influence therapeutic efficacy and long-term outcomes.

MicroRNA Let-7b-5p Targets IGF1R to Inhibit the Progression of Hepatocellular Carcinoma Through the AKT/mTOR Pathway.

Background: Hepatocellular carcinoma (HCC) remains a major global health burden, with microRNA Let-7b-5p (Let-7b-5p) emerging as a potential tumor suppressor. However, its precise role and molecular mechanisms in HCC progression remain unclear, necessitating further investigation.

Methods: We assessed Let-7b-5p expression in HCC versus normal hepatocytes via qRT-PCR and employed functional assays (clone formation, EdU, scratch, Transwell, apoptosis) to examine its effects on proliferation, migration, and cell death.

An AIE-activated fluorescent sensor for one-step concurrent detection and bioimaging of perfluorooctanoic acid and perfluorooctane sulfonate in vitro and in vivo.

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) have emerged as global concerns due to their environmental persistence and associated toxicity. Therefore, accurate detection of PFOA/PFOS in water samples and biological systems is of great importance. In this study, we have designed and synthesized a novel fluorescent sensor ES based on tetraphenyl derivatives for one-step concurrent detection of PFOA and PFOS.

Occupational nanoparticles: major sources, physicochemical properties, multi-organ toxic effects, and associated mechanisms.

Increased exposure to nanoscale particles (NPs) in living and occupational environments has produced various harmful effects in recent years. Owing to their small particle size and physicochemical properties, NPs can evade engineered defenses, exhibit greater toxicity, and affect the physiological functions of multiple organs in the human body through the circulatory system and biological barriers. Therefore, we should pay attention to the multi-organ toxicity effects caused by NPs and their mechanisms.

Light regulates SlCOP1-mediated degradation of SlJAF13, a transcription factor essential for anthocyanin biosynthesis in Aft tomato fruit.

When they are exposed to light, the fruit of tomato plants (Solanum lycopersicum) carrying the dominant gene Anthocyanin fruit (Aft) accumulate anthocyanins. As the regulatory mechanism underlying this accumulation remains unclear, the role played by light in the regulation of SlJAF13, a bHLH transcription factor responsible for anthocyanin biosynthesis in tomato fruit peel, was examined. Gene expression analysis, GUS staining, and immunoblotting assays revealed that light enhanced the stability of SlJAF13 protein at a post-transcriptional level rather than transcriptionally.

Psychological Effects of Noise Exposure on Personnel in Central Sterile Supply Department in China.

Objective: This study aimed to investigate whether noise exposure in hospital sterile supply centers exacerbated the psychological burden on staff, potentially mediated by concerns about physiological health.

Methods: Between July 5 and August 4, 2023, 308 staffs from central sterile supply departments in 30 hospitals in Hainan were chosen by convenient sampling method. They completed self-administered questionnaires to assess noise exposure status and used the Self-Reporting Inventory-90 to quantify the psychological state of staff across three dimensions: somatization, anxiety, and sleep and diet.

Effects of TNF-α on Behaviour and Inflammation in Rats with Rotator Cuff Injury through NGF.

Objective: Rotator cuff injury is a common injury that includes inflammation, partial tearing, or complete tearing of the rotator cuff tendon. In cases of rotator cuff tears (RCTs), Tumor Necrosis Factor-alpha (TNF-α) can trigger the release of nerve growth factor (NGF). TNF-α is an important inflammatory mediator that affects rotator cuff activity and increased NGF expression is observed in RCTs.

Managing Post-Stroke Fatigue Using a Mobile Health Called iHealth After Intracerebral Hemorrhage.

Background: Post-stroke Fatigue (PSF) after Intracerebral Hemorrhage (ICH) is a long-term symptom in stroke survivors. However, the pathogenesis of PSF remains inadequately understood and sufficient evidence-based treatments are lacking. Mobile health (mHealth) technology offers a promising approach to expanding access to high-quality and culturally tailored evidence-based mental care.

Hypoxia-Challenged Pancreatic Adenocarcinoma Cell-Derived Exosomal circR3HCC1L Drives Tumor Growth Via Upregulating PKM2 Through Sequestering miR-873-5p.

Pancreatic adenocarcinoma (PAAD) is a fatal disease with poor survival. Increasing evidence show that hypoxia-induced exosomes are associated with cancer progression. Here, we aimed to investigate the function of hsa_circ_0007678 (circR3HCC1L) and hypoxic PAAD cell-derived exosomal circR3HCC1L in PAAD progression.

Investigation and analysis of magnetic resonance imaging experience and psychological status of patients.

Objective: To analyze factors influencing the service experience of magnetic resonance imaging (MRI) examination and psychological status of patients admitted to a hospital and propose targeted solutions, and optimize the examination process and nursing by analyzing the MRI examination experience and psychological effect on patients.

Methods: The MRI examination rooms of two tertiary general hospitals in Haikou City were sampled at random, and 206 patients who met the study criteria were surveyed on site.

Results: (1) The item with the lowest mean score for patient examination services was whether earplugs were provided to the patient during the examination (B8 = 0.

Imaging, pathology, and diagnosis of solitary fibrous tumor of the pancreas: A case report and review of literature.

Background: A solitary fibrous tumor (SFT) is often located in the pleura, while SFT of the pancreas is extremely rare. Here, we report a case of SFT of the pancreas and discuss imaging, histopathology, and immunohistochemistry for accurate diagnosis and treatment.

Case Summary: A 54-year-old man presented to our hospital with pancreatic occupancy for over a month.

A Case Report of Concurrent Transplant Renal Artery Stenosis, Renal Cell Carcinoma, and Papillary Thyroid Cancer After Renal Transplantation: A Literature Review.

Background: Kidney transplantation is the preferred treatment option for eligible patients with end-stage renal disease. With advanced transplantation technology and novel immunosuppressive agents, kidney transplant recipients survive significantly longer. However, the chance of developing malignant tumors has increased, posing a serious challenge to the survival of transplanted kidneys and patients.

EGFR mediates epithelial‑mesenchymal transition through the Akt/GSK-3β/Snail signaling pathway to promote liver cancer proliferation and migration.

Epidermal growth factor receptor (EGFR) is expressed in various types of cancer and is associated with the malignant biological behavior of cancer cells. In the present study, the expression of EGFR in hepatocellular carcinoma (HCC) tissues and liver cancer cells was detected by immunohistochemical staining, western blotting and immunofluorescence. Furthermore, a lentivirus was transduced into HepG2 liver cancer cells to knock down EGFR expression.

Muc2 mucin O-glycosylation interacts with enteropathogenic Escherichia coli to influence the development of ulcerative colitis based on the NF-kB signaling pathway.

Background: Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine characterized by a compromised intestinal epithelial barrier. Mucin glycans are crucial in preserving barrier function during bacterial infections, although the underlying mechanisms remain largely unexplored.

Methods: A cohort comprising 15 patients diagnosed with UC and 15 healthy individuals was recruited.

Circ_103809 Aggravates the Malignant Phenotype of Pancreatic Cancer Through Modulating miR-197-3p/TSPAN3 Axis.

Pancreatic cancer (PC) is a malignant tumor with insidious clinical manifestations and dismal prognosis. Emerging reports have demonstrated that circRNAs exert pivotal biological function in PC. Here, we investigated the crucial biological role and underlying regulatory mechanisms of differentially expressed circ_103809 in PC.

Bacterial distribution and drug resistance in blood samples of children in Jiangxi Region, 2017-2021.

Objective: This study aims to investigate the distribution and drug resistance of bacteria in clinical blood culture specimens from children in Jiangxi province in recent years and to provide a foundation for preventing and treating bloodstream infection diseases in children.

Methods: The study involved a statistical analysis of the isolation and drug resistance of bacterial strains obtained from blood culture specimens of children in Jiangxi province between 2017 and 2021. The analysis was performed using the WHONET 5.

The transcription factor Foxp1 regulates aerobic glycolysis in adipocytes and myocytes.

In recent years, lactate has been recognized as an important circulating energy substrate rather than only a dead-end metabolic waste product generated during glucose oxidation at low levels of oxygen. The term "aerobic glycolysis" has been coined to denote increased glucose uptake and lactate production despite normal oxygen levels and functional mitochondria. Hence, in "aerobic glycolysis," lactate production is a metabolic choice, whereas in "anaerobic glycolysis," it is a metabolic necessity based on inadequate levels of oxygen.

CD83 B cells alleviate uveitis through inhibiting DCs by sCD83.

Soluble CD83 (sCD83) exerts immunosuppressive functions in many autoimmune diseases, including experimental autoimmune uveitis (EAU), but the cells and mechanisms involved are unclear. This study showed that CD83 B cells were the main sources of sCD83. They alleviated the symptoms of EAU and decreased the percentage of T cells and DCs in the eyes and lymph nodes.

IGF-1R down regulates the sensitivity of hepatocellular carcinoma to sorafenib through the PI3K / akt and RAS / raf / ERK signaling pathways.

Background: Insulin-like growth factor-1 receptor (IGF-1R) promotes cell proliferation and migration and inhibitsapoptosis, all of which can contribute to the development of cancers.

Method: This study investigated the effect and mechanism of IGF-1R in mediating the desensitization of hepatocellular carcinoma (HCC) to sorafenib.

Results: IGF-1R, highly expressed in the HCC cell lines SK-Hep1 and HepG2, promotes cell proliferation, migration, and anti-apoptosis through PI3K / Akt and RAS / Raf / ERK signaling pathways, resulting in HCC resistance to sorafenib.

A model of abusive supervision, self-efficacy, and work engagement among Chinese registered nurses: The mediating role of self-efficacy.

Abusive supervision could negatively influence individual work attitudes, behaviors, and work outcomes. Self-efficacy and work engagement can help to increase nursing performance. But few studies have attempted to determine the specific mechanism between them in China.

Literature review of imaging, pathological diagnosis, and outcomes of metachronous lung and pancreatic metastasis of cecal cancer.

Background: Pancreatic metastasis from colorectal cancer is extremely rare. Here, we report a case of colorectal cancer with lung and pancreatic metastasis and analyze the histopathology, immunohistochemistry, and next-generation sequencing (NGS) to generate a differential diagnosis and treatment of metastatic colon cancer.

Case Presentation: AC1 A 78-year-old man was admitted because of a recently elevated carcinoembryonic antigen.

CircLMTK2 Silencing Attenuates Gemcitabine Resistance in Pancreatic Cancer by Sponging miR-485-5p and to Target PAK1.

Pancreatic cancer (PC) has a high degree of malignancy and poor prognosis, and countless patients have distant metastasis when diagnosed. Gemcitabine (GEM) chemotherapy is one of the main ways of treatment. However, PC cells have been displayed chemoresistance to GEM during treatment.