McsB regulates CtsR thermosensing through peripheral arginine phosphorylation.

When cells sense an elevated temperature in the environment, the bacterial master transcription repressor CtsR becomes phosphorylated and inactivated by the arginine kinase McsB to initiate the expression of heat-shock genes. Here, we utilize a fluorescence intensity shift assay (FISA) based on the photoisomerisation-related fluorescence enhancement (or previously protein-induced fluorescence enhancement, PIFE) effect to monitor the DNA-CtsR-McsB interactions in real time. Our single-molecule analysis reveals that CtsR binds rapidly and stably to the cognate DNA, and that McsB is able to transiently interact with CtsR in situ of the target DNA.

Changes in the genus in patients with autoimmune disease: a systematic review and meta-analysis.

Background: Autoimmune diseases have different pathogenic mechanisms but share underlying patterns of gut microbiome perturbation and intestinal barrier dysfunction. Recent evidence suggests that an arthritogenic strain of causes a local inflammatory response in the gut. Therefore, the aim of this review was to systematically summarize the relationships between and multiple autoimmune diseases.

Camel-Milk-Derived Extracellular Vesicles Mitigate High-Fat-Diet-Induced Obesity by Enhancing Thermogenesis and Modulating Lipid Metabolism in Mice.

Obesity is a significant global health concern linked to chronic diseases, with the thermogenic capacity of brown adipose tissue (BAT) being crucial for obesity treatment. This study investigates the potential of camel-milk-derived extracellular vesicles (mEVs) in mitigating high-fat-diet-induced obesity and metabolic dysfunction in mice. Our results show that oral administration of mEVs significantly reduces body fat percentage and lipid accumulation while lowering serum triglycerides, free fatty acids, and cholesterol levels.

Chemosensitizer Effects of Cisplatin- and 5-Fluorouracil-Treated Hepatocellular Carcinomas by Lidocaine.

Approximately 90% of liver cancer cases are classified as hepatocellular carcinomas (HCCs), with chemotherapy and immunotherapy being the most recommended treatment options. While conventional chemotherapy specifically targets rapidly dividing cancer cells, it can also impact on healthy cells that are proliferating quickly. This collateral damage to healthy cells, along with the potential for cancer cells to develop resistance, presents significant challenges for conventional chemotherapy in liver cancer patients.

Long-term trends in the burden of fracture of vertebral column in China: a comprehensive analysis from 1990 to 2021 based on the global burden of disease study 2021.

Background: Fracture of vertebral column (FVC) is a major public health problem, but the epidemiological investigation of FVC based on large population in China is still limited. This study aims to comprehensively analyze the disease burden of FVC in China from 1990 to 2021, so as to provide insights for public health policies and intervention measures.

Methods: Based on the Global Burden of Disease (GBD) database (1990-2021), the incidence, prevalence, and years lived with disability (YLDs) of FVC were evaluated.

Flourishing chemosynthetic life at the greatest depths of hadal trenches.

Hadal trenches, some of the Earth's least explored and understood environments, have long been proposed to harbour chemosynthesis-based communities. Despite increasing attention, actual documentation of such communities has been exceptionally rare. Here we report the discovery of the deepest and the most extensive chemosynthesis-based communities known to exist on Earth during an expedition to the Kuril-Kamchatka Trench and the western Aleutian Trench using the manned submersible Fendouzhe.

Acupuncture versus sham acupuncture in the treatment of insomnia for patients with Parkinson's disease: a randomized controlled clinical trial.

Background: Parkinson's disease-related insomnia (PD-I) has a profound impact on the overall well-being of patients. The findings of previous studies suggested that acupuncture may potentially improve the quality of sleep-in patients with PD. However, the clinical evidence to assess the effectiveness of acupuncture in managing PD-I remains undisclosed.

Gait dynamics and brain function abnormalities in Parkinson's disease with freezing of gait: a clinical study using resting-state fMRI and wearable devices.

Introduction: Parkinson's disease (PD)-associated freezing of gait (FoG) (PDFoG) refers to episodes where patients feel the urge to move but experience temporary immobility or markedly shortened steps. This leads to frequent falls and, eventually, the loss of walking ability, severely affecting patient quality of life and life expectancy. Despite its clinical importance, the neural mechanisms underlying PDFoG remain unclear.

Foxn3 is required to suppress aberrant ciliogenesis in nonphotoreceptor retinal neurons.

The retinal photoreceptors possess specialized sensory cilia critical for phototransduction while the nonphotoreceptor cells typically exhibit simpler primary cilia or lack them altogether. This dichotomy in ciliary architecture underpins the functional specialization of retinal cell types, but how this dichotomy arises and is maintained remains elusive. This study explores the role of the transcription factor Foxn3 in establishing and maintaining this divergence.

Ultrasonography of the vagus nerve in Parkinson's disease: a systematic review and meta-analysis.

Objective: Previous studies suggest the presence of atrophy of vagus nerve (VN) in patients with Parkinson's Disease (PD). This study aims to comprehensively evaluate the changes in the VN detected by ultrasonography in PD patients.

Methods: A systematic review and meta-analysis was conducted.

Potential protective role of high mediterranean diet adherence on systemic lupus erythematosus: mediation by liver-kidney biomarkers in the UK biobank cohort.

Objectives: Despite advances in the treatment of systemic lupus erythematosus (SLE), the complexity and high mortality of the disease drive the need to explore primary prevention strategies. The Mediterranean diet (MD) has shown benefits for various diseases, but its role in SLE remains controversial, lacking evidence from large prospective studies. This study aims to investigate the association between MD and SLE risk and to analyze the potential mediating role of MD-related biomarkers.

A systematic review: Brain age gap as a promising early diagnostic biomarker for Alzheimer's disease.

Alzheimer's disease (AD) is a progressive neurodegenerative disorder for which there is currently no cure, and its incidence is on the rise. Early detection is essential for timely intervention and slowing the progression of the disease. While the brain structures of healthy aging individuals change gradually, the aging trajectories in AD patients deviate significantly.

Oligogalacturonides Operate as Endogenous Elicitors to Regulate Aluminum Tolerance in Pea (Pisum sativum).

Aluminum (Al) toxicity is a major limiting factor leading to crop yield reduction in acidic soils. The pectic polysaccharides, key components of plant cell walls, are considered the primary binding site for Al ions. Oligogalacturonide (OGA), the oligomers of alpha-1,4-linked galacturonosyl residues originating from the degradation of cell wall pectin (homogalacturonan), are able to elicit defense responses and protect plants against biotic stress, such as pathogen infections.

A battery-free wearable sweat lactate sensing patch for assessing muscle fatigue and recovery.

Long-term sports and rehabilitation training require effective monitoring of muscle fatigue and recovery. Blood lactate fails to accurately reflect muscle fatigue level over extended period. Sweat lactate is a promising alternative, but its potential for assessing multi-day muscle fatigue remains unexplored.

Fatal risk factors and the efficacy of glucocorticoid therapy in severe fever with thrombocytopenia syndrome: a multicenter retrospective cohort study.

Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne infectious disease characterized by rapid progression and high mortality. Glucocorticoids (GCs) can be used as anti-inflammatory agents for SFTS, but no standardized protocols have been proposed.

Methods: A total of 901 patients with SFTS diagnosed at two hospitals between July 2017 and October 2023 were included in this retrospective cohort study.

Risk factors for coronary artery calcification in Chinese patients undergoing maintenance hemodialysis: a meta-analysis.

Background: Chronic kidney disease (CKD) progression to end-stage renal disease (ESRD) increases cardiovascular disease (CVD) risk, with coronary artery calcification (CAC) affecting 70% of Chinese maintenance hemodialysis (MHD) patients. Prolonged MHD treatment is linked to calcium-phosphorus imbalance and accelerated CAC progression. However, conflicting findings on CAC risk factors persist due to methodological heterogeneity in existing studies.

ToxDL 2.0: Protein toxicity prediction using a pretrained language model and graph neural networks.

Motivation: Assessing the potential toxicity of proteins is crucial for both therapeutic and agricultural applications. Traditional experimental methods for protein toxicity evaluation are time-consuming, expensive, and labor-intensive, highlighting the requirement for efficient computational approaches. Recent advancements in language models and deep learning have significantly improved protein toxicity prediction, yet current models often lack the ability to integrate evolutionary and structural information, which is crucial for accurate toxicity assessment of proteins.

[Spatio-temporal Characteristics and Obstacle Factors of the Coupling Synergy Between New-type Urbanization and Low-carbonization of the Living Energy Consumption of Urban Residents].

Clarifying the dynamics and mechanism of the coupling and coordination relationship between new-type urbanization and the low-carbonization of the living energy consumption of urban residents can provide basic data and theoretical support for the national low-carbon economy and other high-quality development strategies from the perspective of urbanization. The entropy method, coupling coordination model, kernel density estimation, and obstacle model were used to investigate the spatial and temporal characteristics, coupling coordination, and obstacle factors of the two systems of China's new-type urbanization and the low-carbonization of the living energy consumption of urban residents from 2005 to 2021. The results showed that： ① The overall level of new-type urbanization and low-carbonization of the living energy consumption of urban residents showed an upward trend, and both had obvious spatial differentiation characteristics.

Research progress on the application of virtual reality technology in patients with Duchenne muscular dystrophy.

Duchenne muscular dystrophy is a common hereditary muscular dystrophy, virtual reality technology as an emerging therapeutic method has been gradually applied to the rehabilitation treatment of Duchenne muscular dystrophy patients. This paper reviews the current management status of Duchenne muscular dystrophy patients, the application effect of virtual reality technology in the rehabilitation treatment of Duchenne muscular dystrophy patients, and points out the challenges faced by the application of virtual reality technology in the field of Duchenne muscular dystrophy, aiming to provide a basis for the care of muscular dystrophy patients.

Recurrent Keloids: Risk Factors and Treatment Efficacy.

Background: The protocols for managing recurrent keloids remain contentious. This retrospective study analyzed both recurrent and new keloids to identify risk factors for recurrence and to evaluate various treatment strategies for recurrent keloids.

Methods: Patients were enrolled from July 2021 to January 2024 at the Plastic Surgery Department of Tianjin Hospital.

Remimazolam induced cytotoxicity mediated through multiple stress pathways and acted synergistically with tyrosine kinase inhibitors in hepatocellular carcinoma.

The primary treatment for hepatocellular carcinoma (HCC) involves surgical removal of the primary tumor, but this creates a favorable environment for the proliferation and spread of residual and circulating cancer cells. The development of remimazolam-based balanced anesthesia is crucial for future antitumor applications. It is important to understand the mechanisms of cytotoxicity for HCC in detail.

Selective Hydrogenolysis of Biomass-Derived Aromatic Alcohols over 2D-MoCO MXene by a Reversible Redox-Relay Mechanism.

Selective CH-OH hydrogenolysis of biomass-derived aromatic alcohols to produce methyl aromatics is crucial for synthesizing sustainable fuels and chemicals; yet traditional metal-acid bifunctional catalysis possesses significant challenges owing to its complex reaction network. Herein, a 2D-MoCO MXene was fabricated, demonstrating an efficient hydrogenolysis of 5-hydroxymethyl furfural into 5-methyl furfural, achieving an impressive yield (99.5 %) at the mild reaction temperature of 90 °C.

Response of an obligate CAM plant to competition and increased watering intervals.

Climate change has exacerbated precipitation variability, profoundly impacting vegetation dynamics and community structures in arid ecosystems. There remains a notable knowledge gap regarding the ecological effects of altered precipitation on crassulacean acid metabolism (CAM) plants and their interactions with other photosynthetic types. This study investigated the response of the typical obligate CAM plant Orostachys fimbriata to extended watering intervals (WI4-WI8) and various competitive patterns (M-M) with the C grass Melilotus officinalis and the C grass Setaria viridis through greenhouse experiments.

Microorganism-mediated production of anthocyanins: Current progress and future prospects.

Anthocyanins are a type of water-soluble pigments widely distributed in colorful plants, which have been extensively used in food and cosmetics industry. The current production of anthocyanins heavily depends on extraction from plant materials, which leads to low purity and inconsistency among batches. Compared with conventional extraction, microorganism-mediated production of anthocyanins has advantages such as a short production cycle, high purity, low waste production, low energy requirements, and consistency between different batches.

Swords and shields: the war between Liberibacter asiaticus and citrus.

Citrus Huanglongbing (HLB) represents a significant threat to the citrus industry, mainly caused by the phloem-limited bacterium Liberibacter asiaticus (Las). In this review, we summarize recent advances in understanding the relationship between citrus and Las, particularly examining the functions of Sec-dependent effectors (SDEs) and non-classically secreted proteins (ncSPs) in virulence, as well as their targeted interactions with citrus. We further investigate the impact of SDEs on various physiological processes, including systemic acquired resistance (SAR), reactive oxygen species (ROS) accumulation, vesicle trafficking, callose deposition, cell death, autophagy, chlorosis and flowering.