A Hybrid Protein-Oxygen Nanomedicine Overcomes Osimertinib Resistance in NSCLC via HIF-1α/VEGF/EGFR Inhibition.

Purpose: Osimertinib, established as the frontline treatment for advanced non-small cell lung cancer (NSCLC), can effectively prolong progression-free survival. However, it faces the problem of reduced treatment persistence due to acquired drug resistance. Meanwhile, tumor hypoxia is also a key driver of drug resistance.

Investigation and risk factor analysis of anxiety and depression in patients with lower extremity arteriosclerosis obliterans.

Background: The prevalence of lower extremity arteriosclerosis obliterans (LEASO) in China is progressively increasing with the increment in age. Certain patients may be diagnosed with lower extremity arterial stenosis and occlusion that resist complete curative efforts, which will invariably impose a substantial psychological strain on them.

Aim: To investigate the anxiety and depression conditions among patients with LEASO and analyze the associated risk factors.

prdm1a drives a fate switch between hair cells of different mechanosensory organs.

Vertebrate inner ear mechanosensory hair cells detect sound and gravitational forces. Additionally, fishes have homologous lateral line hair cells in the skin that detect water vibrations for orientation and predator avoidance. Hair cells in the lateral line and ear of fishes and other non-mammalian vertebrates regenerate readily after damage, but mammalians lack this ability, causing deafness and vestibular defects.

Stem and progenitor cell proliferation are independently regulated by cell type-specific cyclinD genes.

Regeneration and homeostatic turnover of solid tissues depend on the proliferation of symmetrically dividing adult stem cells, which either remain stem cells or differentiate based on their niche position. Here we demonstrate that in zebrafish lateral line sensory organs, stem and progenitor cell proliferation are independently regulated by two cyclinD genes. Loss of ccnd2a impairs stem cell proliferation during development, while loss of ccndx disrupts hair cell progenitor proliferation but allows normal differentiation.

Two Distinct Oxidation Dispersion Mechanisms in Pd-CeO_{2} Mediated by Thermodynamic and Kinetic Behaviors of Highly Dispersed Pd Species.

Using environmental scanning transmission electron microscopy and a global optimization algorithm, we unraveled the preoxidation dispersion and direct dispersion mechanisms in Pd-CeO_{2}(100) systems, mediated by the behaviors of highly dispersed Pd species. At lower temperatures, Pd nanoparticles first undergo oxidation, followed by the dispersion of PdO nanoparticles. In contrast, at higher temperatures, Pd nanoparticles directly convert into highly distributed cationic Pd species.

drives a fate switch between hair cells of different mechanosensory organs.

Vertebrate mechanosensory hair cells (HCs) in the ear detect sound and gravitational forces. Additionally, fish have homologous lateral line HCs in the skin that detect water vibrations for orientation and predator avoidance. HCs in fish and other non-mammalian vertebrates regenerate to restore function after damage, but mammalian HCs lack this ability, causing deafness and vestibular defects.

Liquid-liquid phase separation of DDX3X: mechanisms, pathological implications, and therapeutic potential.

DDX3X is a multifunctional DEAD-box RNA helicase that plays pivotal roles in diverse biological processes, including RNA metabolism, translation regulation, and the progression of various diseases such as intellectual disabilities, inflammation, viral infections, and cancer. Over the past decades, extensive research has unveiled the physiological and pathological functions of DDX3X. However, the precise molecular mechanisms underlying these processes remain incompletely understood, limiting the development of targeted therapies for DDX3X-related diseases.

Interface engineering to regulate oxidation dynamics of supported nanoparticles.

Understanding the oxidation of metal nanoparticles is crucial for various applications, particularly in heterogeneous catalysis, such as catalytic oxidation reactions, where metal nanoparticles are typically dispersed on supports. However, the dynamics of interaction between nanoparticles and oxygen, especially under the influence of supporting materials, remain poorly understood, significantly hindering the precise comprehension and regulation of nanoparticle oxidation dynamics. Here, we elucidate two distinct oxidation dynamics in supported nanoparticles using aberration-corrected environmental (scanning) transmission electron microscopy (E(S)TEM), i.

Expression and Effects of FUS/Sfrp5/Wnt5 in Atherosclerosis Development.

Objective: Atherosclerosis (AS) contributes significantly to the development of cardiovascular disease. Despite recent advances in medical treatment, the outcomes for patients with AS are still unsatisfactory. This study aims to enhance our understanding of AS and pave the way for the development of more effective therapeutic approaches to improve patient outcomes and ultimately reduce the burden of this devastating condition.

Noninvasive early prediction of preeclampsia in pregnancy using retinal vascular features.

Preeclampsia (PE), a severe hypertensive disorder during pregnancy, significantly contributes to maternal and neonatal mortality. Existing prediction biomarkers are often invasive and expensive, hindering their widespread application. This study introduces PROMPT (Preeclampsia Risk factor + Ophthalmic data + Mean arterial pressure Prediction Test), an AI-driven model leveraging retinal photography for PE prediction, registered at ChiCTR (ChiCTR2100049850) in August 2021.

Percutaneous transluminal angioplasty with stenting for the treatment of lower limb arteriosclerosis obliterans.

Introduction: With the advancement of minimally invasive techniques, percutaneous transluminal angioplasty with stenting (PTAS) has emerged as a significant treatment approach for lower limb arteriosclerosis obliterans (ASO).

Aim: The aim of this study was to evaluate the clinical efficacy of PTAS in patients with lower limb ASO.

Materials And Methods: A total of 96 ASO patients admitted to our hospital between January 2021 and December 2022 were included in this study.

Charged Insulating Skeleton-Enabled Deep Deposition and Robust Interface for Stable Lithium Metal Anodes.

Conductive 3D collectors for lithium metal anodes always lead to the direct deposition of lithium on top of the skeleton, which contributes to aggressive volume change and an unstable interface layer. Herein, we design a three-dimensional collector composed of an insulating glass fiber skeleton and conductive copper substrate (NGF@Cu) to guide a bottom-up deep deposition of lithium metal. This composite structure with abundant pores realizes a directed ion-electron path and deep lithium deposition, fully harnessing its internal space to accommodate lithium.

The combination of immune checkpoint inhibitor and chemotherapy may be efficacious for advanced non-small cell lung cancer with near-loop insertions of exon 20: A retrospective analysis.

ObjectiveThe sensitivity of immune checkpoint inhibitors (ICIs) as monotherapy is low in non-small cell lung cancer (NSCLC) patients with epidermal growth factor receptor () exon 20 insertion mutations (ex20ins). This study aims at investigating the effectiveness of the combination of ICI and chemotherapy (ICI-combined regimen) in a real-world population of NSCLC patients harboring near-loop insertions of exon 20.MethodsWe conducted a retrospective study of advanced NSCLC with ex20ins from April 2016 to March 2021 at Guangdong Provincial People's Hospital, Southern Medical University, China.

Luminescent Metal-Organic Framework with Negative Electrostatic Pores for Highly Selective GDP Sensing.

Electrostatic potential (ESP) plays an essential role in studying interactions among molecules. Developing probe materials capable of selectively detecting analytes by aligning their molecular ESP with the electrostatic interaction of the host probe material is critically important for identifying analogous analytes; however, relevant research is extremely lacking. In this work, we synthesized a luminescent metal-organic framework (LMOF, Cd-DBDP) featuring negative electrostatic pore environments achieved by incorporating numerous electronegative oxygen atoms and N-containing aromatic rings from organic linkers.

Correlation of neutrophil percentage-to-albumin ratio with lung function in American adults: a population study.

Background: Chronic respiratory diseases pose a significant threat to global health, underscoring the urgent need for effective preventative and therapeutic interventions. The neutrophil-to-albumin ratio (NPAR), an emerging biomarker for inflammation and nutritional status, has shown promising associations with respiratory health, necessitating an investigation into its potential for predicting lung function decline.

Objective: This study aimed to delineate the relationship between the NPAR and pulmonary function within a sample of the American adult population and assess the viability of the NPAR as a prognostic indicator for compromised lung function.

Integrated bioinformatics analysis of the effects of chronic pain on patients with spinal cord injury.

Background: Spinal cord injury (SCI) poses a substantial challenge in contemporary medicine, significantly impacting patients and society. Emerging research highlights a strong association between SCI and chronic pain, yet the molecular mechanisms remain poorly understood. To address this, we conducted bioinformatics and systems biology analyses to identify molecular biomarkers and pathways that link SCI to chronic pain.

SNORD113-114 cluster maintains haematopoietic stem cell self-renewal via orchestrating the translation machinery.

Haematopoietic stem cells (HSCs) self-renew and differentiate to replenish the pool of blood cells, which require a low but finely tuned protein synthesis rate. Nonetheless, the translatome landscape in HSCs and how the translation machinery orchestrates HSC self-renewal remain largely elusive. Here we perform ultra-low-input Ribo-seq in HSCs, progenitor and lineage cells, and reveal HSC-specific translated genes involved in rRNA processing.

PEARL: A Multicenter Phase 2 Study of Lorlatinib in Patients with ALK-Rearranged NSCLC and Central Nervous System Disease.

Background: Patients with ALK-rearranged non-small cell lung cancer (ALK+ NSCLC) with symptomatic brain (BM) and leptomeningeal (LM) metastases are underrepresented in clinical trials due to poor performance status. Additionally, the need for improved and validated assessment criteria for evaluating central nervous system (CNS) response remains critical. Lorlatinib has demonstrated systemic activity in patients with ALK+ NSCLC.

The effects of plasma from patients with active thyroid-associated orbitopathy on the survival and inflammation of melanoma-associated fibroblasts.

Background: Plasma from patients with active thyroid-associated orbitopathy (TAO-A) could cause inflammation to fibroblasts, and such a mechanism was explored in the context of melanoma.

Methods: Plasma samples collected from TAO-A patients and healthy control (HC) were primarily co-cultured with the melanoma-associated fibroblasts (MAFs) derived from melanoma patients. The survival and inflammation of the co-cultured MAFs were measured after confirming the levels of pro-inflammatory cytokines.

Effect of resting heart rate on the risk of metabolic syndrome in adults: a dose-response meta-analysis.

Aims: Given the notable rise in the prevalence of metabolic syndrome (MS) in China, it is urgent to identify early screening indicators. Extensive dose-response meta-analyses have been conducted to investigate the association between resting heart rate (RHR) and MS, and additional relevant studies have been updated in the last five years. Therefore, this paper aims to update the results of previous meta-analyses.

Stem and progenitor cell proliferation are independently regulated by cell type-specific genes.

Regeneration and homeostatic turnover of solid tissues depend on the proliferation of symmetrically dividing adult stem cells, which either remain stem cells or differentiate based on their niche position. Here we demonstrate that in zebrafish lateral line sensory organs, stem and progenitor cell proliferation are independently regulated by two genes. Loss of impairs stem cell proliferation during development, while loss of disrupts hair cell progenitor proliferation but allows normal differentiation.

Tumor immune microenvironment of NSCLC with EGFR exon 20 insertions may predict efficacy of first-line ICI-combined regimen.

Objectives: Non-small cell lung cancer (NSCLC) patients with exon 20 insertion mutations (ex20ins) of the epidermal growth factor receptor (EGFR) were resistant to monotherapy of immune checkpoint inhibitor (ICI). However, recent reports have shown that the combination of ICI and chemotherapy (ICI-combined regimen) exhibited certain efficacy for NSCLC with EGFR ex20ins. The mechanisms behind this phenomenon have not been thoroughly clarified.

Spatial Identification of Mott-Schottky Effect at Electrocatalytic Pd/Metal Oxide Interfaces for the Oxygen Reduction Reaction.

To elucidate the microstructure and charge transfer behavior at the interface of Pd/metal oxide semiconductor (MOS) catalysts and systematically explore the crucial role of the Mott-Schottky effect in the oxygen reduction reaction (ORR) electrocatalysis process, this study established a testing system for spatially identifying Mott-Schottky effects and electronic properties at Pd/MOS interfaces, leveraging highly sensitive Kelvin probe force microscopy (KPFM). This system enabled visualization and quantification of the surface potential difference and Mott-Schottky barrier height (Φ) at the Pd/MOS heterojunction interfaces. Furthermore, a series of Pd/MOS Mott-Schottky catalysts were constructed based on differences in work functions between Pd and n-type MOS.

A CeO (100) surface reconstruction unveiled by in situ STEM and particle swarm optimization techniques.

The reconstruction of the polar CeO (100) surface has been a subject of long-standing debates due to its complexity and the limited availability of experimental data. Herein, we successfully reveal a CeO (100)-(4 × 6) surface reconstruction by combining in situ spherical aberration-corrected scanning transmission electron microscopy, density functional theory calculations, and a particle swarm optimization-based algorithm for structure searching. We have further elucidated the stabilizing mechanism of the reconstructed structure, which involves the splitting of the filled Ce(4f) states and the mixing of the lower-lying ones with the O(2p) orbitals, as evidenced by the projected density of states.