Novel triple-target panels utilizing methylation-sensitive restriction enzyme-based quantitative PCR for detecting advanced cervical precancers and cancers among high-risk HPV-positive women.

Background: Optimal triage option for high-risk HPV-positive (hrHPV) women remains uncertain. We aimed to utilize methylation-sensitive restriction enzyme-based quantitative PCR (MSRE-qPCR) technique and develop triple-target human gene methylation panels to improve detection of advanced cervical precancers and cancers (CIN3 +) among hrHPV women.

Methods: Eighteen candidate genes were detected by MSRE-qPCR in cervical samples from hrHPV women.

A Long-Lasting Rechargeable Seawater Zn-Air Battery Enabled by Vacancy-Driven CoO-MoS Bifunctional Cathodes.

The global energy crisis and unsustainable reliance on fossil fuels make it particularly urgent to develop efficient renewable energy storage solutions. Aqueous zinc-air batteries (ZABs) have become extremely promising candidates due to their low cost, abundant surface resources, inherent safety, and high theoretical energy density. This study explores the feasibility of using seawater as an electrolyte for ZABs, with the aim to reduce costs and alleviate competition for limited freshwater resources.

Causal links between long-term exposure to the chemical constituents of PM and lung cancer mortality in southern China.

While studies reported the impact of particulate matter (PM) on lung cancer mortality, evidence for PM constituents remains limited. We collected data from a cohort of 182,009 participants enrolled during 2013-2015 until 2020 in Guangzhou, China. We explored the impact of individual PM constituents on lung cancer mortality using marginal structural Cox proportional hazard models, an established causal inference approach, and evaluated their joint effect using quantile-based g-computation.

F-box proteins at the crossroads of ubiquitination and tumor immunity: regulatory networks and immunotherapy strategies.

As critical substrate-recognition subunits of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex, F-box proteins mediate the ubiquitination and subsequent degradation of specific target proteins, playing pivotal roles in cell cycle regulation, signal transduction (e.g., MAPK and NF-κB pathways), and immune homeostasis.

HPV genotyping agreement between paired cervical cytological sample and biopsy across lesion severity and vaccination.

Background: Cytological samples are genotyped to inform clinical management of HPV-infected women due to their accessibility. Conversely, HPV genotypes identified in biopsies are deemed directly associated with cervical lesions. Thus, investigating genotyping agreement between these two sample types and potential influence of lesion severity and vaccination status on their degree of concordance is essential for understanding their diagnostic reliability.

HPV integration status conversion and CIN2 + cancer risk stratification based on HPV integration levels among HPV integration-positive women: a 1-year follow-up study.

Background: HPV integration is a crucial genetic step in cervical carcinogenesis and the level of HPV integration increases with the grade of precancerous lesion. This study aimed to conduct risk stratification based on HPV integration levels and HPV integration status conversion among HPV integration-positive women after 1 year of follow-up.

Methods: This prospective cohort study was conducted in Tongji Hospital between June 2020 and August 2022 and included 1297 consecutive HPV-positive women.

The effect of socioeconomic status on postpartum depression: a parallel mediation model.

Background: Previous studies have proposed that socioeconomic status (SES) can affect postpartum depression (PPD) through the mediators of satisfaction with material needs, healthcare, maternity leave, and postpartum social support. However, empirical data to validate these propositions has been lacking. This study aims to examine if all the four mediators can significantly mediate the effects of SES on PPD, as well as to compare the effect sizes of these mediators using a parallel mediation model.

Association of Genetically Predicted Obesity and Stool Frequency: Evidence From an Observational and Mendelian Randomization Study.

Background/aims: Obesity is associated with several gastrointestinal (GI) disorders and has been identified as a potential risk factor for various GI symptoms. Bowel frequency is an important indicator of bowel function. However, the causal link between obesity and gastrointestinal motility remains uncertain.

Lipid and polyphenol removal on the structural, physico-chemical and technological properties of passion fruit epicarp flour.

The effects of defatting and dephenolization treatments on the structural, physico-chemical and technological properties of passion fruit epicarp flour (PFEF) were evaluated in this research. The FT-IR, SEM and XRD results illustrated that defatted and defree phenolics did not change the basic chemical structure of PFEF, while debound phenolics treatment hydrolyzed the ester bonds between dietary fiber and polyphenols and hydrolyzed the amorphous region of dietary fiber, resulting in self-assembly into a larger aggregate with a compact texture. All three kinds of treatment could improve the flowability of passion fruit epicarp flour to some extent.

Loss of survivorship in nasopharyngeal carcinoma attributable to fine particulate matter and its constituents.

Background: Nasopharyngeal carcinoma (NPC) is a prevalent malignant tumor in East Asia, particularly impacting China. The association between multiple constituents of fine particulate matter (PM) and the survival time of NPC patients remains unclear, which poses a challenge for targeted public health interventions.

Methods: An accelerated failure-time model with a 12-year cohort design was used to analyze the impact of long-term PM and its constituents on the survival time of 1492 NPC patients.

CRISPR/Cas9-mediated editing of the melanization gene ebony in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata.

The melanization process, which is essential for the proper functioning of the cuticle, has been extensively investigated for its enzymatic roles and physiological effects. Henosepilachna vigintioctopunctata, a significant pest species, presents considerable economic threats. However, due to the variable efficiency of RNA interference for genetic manipulation, establishing a CRISPR/Cas9 system is crucial for providing a more precise and reliable method for functional genomics in this non-model insect.

[Expert consensus on ethical requirements for artificial intelligence (AI) processing medical data].

As artificial intelligence technology rapidly advances, its deployment within the medical sector presents substantial ethical challenges. Consequently, it becomes crucial to create a standardized, transparent, and secure framework for processing medical data. This includes setting the ethical boundaries for medical artificial intelligence and safeguarding both patient rights and data integrity.

Transcription factor MEF2D regulates aberrant expression of ACSL3 and enhances sorafenib resistance by inhibiting ferroptosis in HCC.

Background: Sorafenib is a first-line treatment for hepatocellular carcinoma (HCC); however, acquired resistance often results in a poor prognosis, indicating a need for more effective therapies. Sorafenib induces cell death through an iron-dependent mechanism known as ferroptosis, which is closely associated with the onset and progression of HCC.

Methods: This study investigated the role of ACSL3 in sorafenib resistance and ferroptosis in HCC.

The Active Ingredients of Semantic Naming Treatment: Evidence From Mandarin-English Bilingual Adults With Aphasia.

Purpose: Following the Rehabilitation Treatment Specification System (RTSS) framework, the current study investigated the active ingredients in the modified semantic feature analysis (mSFA) targeting either noun or verb retrieval in Mandarin-English bilingual adults with aphasia (BWA).

Method: Twelve Mandarin-English BWA completed mSFA treatment for nouns and verbs. Eight of them completed both noun and verb treatment, while four completed either type of treatment.

Smoking cessation and mortality risk reduction in older adults with long-term smoking history.

Background: The association between smoking cessation and decreased mortality existed among former smokers has been well documented. However, evidence is limited for smokers with long-term exposure. This study aims to quantify the association between smoking cessation and mortality by years since quitting in older adults with long-term smoking history.

Analysis of Related Factors Influencing Hypertension Classification among Centenarians in Hainan, China.

Background: As a population ages, blood pressure levels gradually increase, leading to a higher incidence of hypertension and increased cardiovascular diseases risk. This study examines factors affecting hypertension grading among centenarians in the Hainan Province.

Methods: Data from 2014 to 2016 were accessed from the cross-sectional database "Hypertension Levels and Epidemiological Characteristics of the Elderly and Centenarians in Hainan province of China".

Comparative evaluation of two clinical sampling techniques for HPV detection in male genital sites: a randomized controlled study.

Aims: The optimal sampling methods for detecting human papillomavirus (HPV) in male genital sites remain unclear. This study aimed to assess the performance, acceptability, and comfort of two sampling techniques for male genital HPV detection.

Methods And Results: A total of 490 men aged 18-45 were randomly assigned in a 1:1 ratio to undergo either the rub-brush (nail file followed by swab) or brush-only method (swab only) for sampling at external genitalia sites (PGS) and perineum/perianal (PA) sites.