Prognostic Impact of Non-Impacted Third Molar Extraction on Non-Surgical Periodontal Therapy for Adjacent Second Molars: A Prospective Split-Mouth Study.

Aims: To compare the outcomes of non-surgical periodontal therapy (NSPT) alone and NSPT plus adjacent non-impacted third molar (N-M3) removal on second molars (M2s).

Materials And Methods: In this prospective split-mouth study, 40 patients with periodontitis who had N-M3s on both sides of the same jaw were enrolled. For each patient, a full-mouth NSPT was performed, with one N-M3 extracted (N-M3-) and the other N-M3 retained (N-M3+).

Assessing biomass retention-driven enhancement of bioenergy and nutrient recovery potential in anaerobic membrane bioreactors for sustainable sewage sludge management.

Sustainable management of sewage sludge (SewS) requires efficient strategies for energy recovery and nutrient reutilization. An anaerobic membrane bioreactor (AnMBR) and a continuous stirred tank reactor (CSTR) were operated to evaluate the advantages of membrane-integrated systems in SewS management. The AnMBR achieved a methane yield of 313.

Fouling dynamics varied by filtration cycles in high-solid anaerobic membrane bioreactors for sewage sludge treatment.

Anaerobic membrane bioreactors (AnMBRs) are highly effective in treating sewage sludge (SewS) by promoting the rate-limiting hydrolysis process through biosolids concentration. The treatment of SewS in this study by the high-solid AnMBR achieved an exceptional COD removal efficiency of 99.1 % and a substantial bioenergy yield of 157.

Functional avidity enhancement of a T-cell receptor targeting the KRAS cancer neoantigen.

Engineered T cell receptors (TCRs) targeting neoantigens represent a transformative approach in cancer immunotherapy, yet their clinical potential is limited by low natural TCR avidity and the risk of off-target toxicity from over-engineered TCRs with excessive high-affinity. Here, we developed a TCR engineering platform to enhance the functional avidity of a TCR targeting the KRAS G12D mutation (KRAS) while avoiding reactivity to the wild-type (WT) peptide. We separately constructed CDR3α- and CDR3β-focused TCR libraries derived from an HLA-A*11:01-restricted KRAS-specific TCR and screened them using alternating positive and negative selection: KRAS-pulsed antigen-presenting cells (APCs) drove functional avidity, while KRAS-pulsed APCs eliminated cross-reactive clones.

Case Report: Acute superior mesenteric artery embolism combined with abdominal aortic aneurysm in an elderly female patient.

Background: Both acute superior mesenteric artery embolism (ASMAE) and abdominal aortic aneurysm (AAA) are insidious conditions that can lead to fatal outcomes. The coexistence of ASMAE and AAA in a single patient is rare.

Methods And Results: A 78-year-old female patient presented to our hospital due to abdominal pain for 10 h, with a diagnosis of AAA 2 h prior.

Screening and identification Hub genes associated with immune cell infiltration and critical biomarkers in osteosarcoma.

Purpose: Osteosarcoma (OS) exhibits limited immune cell infiltration that directly contributes to poor prognosis. This study sought to screen and identify pivotal biomarkers of OS immune infiltration and early diagnosis of OS.

Methods: The immune cell infiltration profiles with transcriptome sequencing data from 88 OS samples were explored with CIBERSORT algorithm.

Robust aeration coefficient control based on pH feedback towards stable NO-N/NH-N ratio in large pilot-scale partial nitritation reactor treating food waste digestate.

Two-stage partial nitritation/anammox (PNA) system is a promising technology for treating food waste digestate (FWD). Maintaining a stable NO-N/NH-N ratio in partial nitritation (PN) is essential for the successful implementation of two-stage PNA. Although the aeration coefficient (AC) is a key regulatory parameter, its stability is challenged by fluctuations in chemical oxygen demand (COD).

Tumour-infiltrating immune cells as a novel prognostic model for bladder cancer.

Bladder cancer (BLCA) is the tenth most commonly diagnosed cancer and poses a significant challenge due to its complexity and associated high morbidity and mortality rates in the absence of optimal treatment. The tumor microenvironment (TME) is recognized as a critical factor in tumor initiation, progression and therapeutic response, and offers numerous potential targets for intervention. A comprehensive understanding of immune infiltration patterns in BLCA is essential for the development of effective prevention and treatment strategies.

Unveiling purine metabolism dysregulation orchestrated immunosuppression in advanced pancreatic cancer and concentrating on the central role of NT5E.

Background: The dismal efficacy of immunotherapy for Pancreatic cancer (PC) can be predominantly ascribed to its distinctive cold-tumor properties. The by-products of purine metabolic reprogramming are extensively engaged in tumor immune modulation, influencing the functions and recruitment of immune cells and molding an immune microenvironment that is propitious for tumor growth.

Methods: We harnessed single-cell transcriptomics and spatial transcriptomics to concurrently analyze the purine metabolism (PM) features of the PC microenvironment.

Machine learning based predictive model and genetic mutation landscape for high-grade colorectal neuroendocrine carcinoma: a SEER database analysis with external validation.

Background: High-grade colorectal neuroendocrine carcinoma (HCNEC) is a rare but aggressive subset of neuroendocrine tumors. This study was designed to construct a risk model based on comprehensive clinical and mutational genomics data to facilitate clinical decision making.

Methods: A retrospective analysis was conducted using data from the Surveillance, Epidemiology, and End Results (SEER) database, spanning 2000 to 2019.

Presence of nonimpacted third molars affect the response of neighboring teeth to nonsurgical periodontal therapy.

Background: This study focused on whether the effectiveness of nonsurgical periodontal therapy (NSPT) on second molars (M2s) varies with the status of adjacent nonimpacted third molars (N-M3s).

Methods: In this retrospective cohort study, 776 regions from 268 patients were evaluated. Based on the adjacent N-M3 status, quadrants with M2s were categorized into three groups: N-M3 group (present N-M3), A-M3 group (absent M3), and R-M3 group (removed N-M3).

Specific molecular imaging of BALB/c model mice with Graves' ophthalmopathy based on high expression of insulin-like growth factor 1 receptor.

Objective: At present, most of the targeted imaging based on insulin-like growth factor 1 receptor (IGF-1R) is for tumor research, and there is no IGF-1R-targeted imaging for Graves' ophthalmopathy(GO). This study aims to develop a peptide probe, Tc-Z-GGGC, targeting the IGF-1R, and to achieve specific imaging in Graves' disease (GD) animal models exhibiting GO.

Methods: 99mTc-ZIGF1R:4551-GGGC probe was synthesized using a direct labeling method and its labeling efficiency assessed via instant thin-layer chromatography (ITLC).

Melittin suppresses ovarian cancer growth by regulating SREBP1-mediated lipid metabolism.

Background: Melittin, a major peptide component of bee venom, has demonstrated promising anti-cancer activity across various preclinical cell models, making it a potential candidate for cancer therapy. However, its molecular mechanisms, particularly in ovarian cancer, remain largely unexplored. Ovarian cancer is a life-threatening gynecological malignancy with poor clinical outcomes and limited treatment options.

Effects of acupuncture at limb Acupoints-Guangming (GB37) on UDVA, CS, and EEG microstate in myopia.

Introduction: Acupuncture is beneficial in improving visual function for myopi periocular acupoints Taiyang can improve contrast sensitivity (CS). In this study, we aim to further investigate the impact of acupuncture at the limbs acupoints-Guangming(GB37) acupoint on visual function, and the neural mechanism of acupuncture at the GB37 acupoint improving visual function through electroencephalography (EEG) microstate.

Methods: A total of 22 myopia were recruited.

A pilot study of precision treatment for patients with lung cancer pain by Longteng Tongluo recipe using serum genomics.

Objective: To investigate the efficacy of Longteng Tongluo recipe (, LTTL) combined with three-step analgesia for the treatment of lung cancer pain, and the changes in serum miRNA expressions before- and after treatment with LTTL and its correlation with lung cancer pain. The possible mechanism underlying LTTL effects on the treatment of lung cancer pain was conducted.

Methods: The pilot study was conducted at the oncology ward of the Yueyang Hospital and the Longhua Hospital between March 2018 and October 2019.

The role of KLF5 in gut microbiota and lung adenocarcinoma: unveiling programmed cell death pathways and prognostic biomarkers.

Lung adenocarcinoma (LUAD) is the most important subtype of lung cancer. It is well known that the gut microbiome plays an important role in the pathophysiology of various diseases, including cancer, but little research has been done on the intestinal microbiome associated with LUAD. Utilizing bioinformatics tools and data analysis, we identified novel potential prognostic biomarkers for LUAD.

Pyroptotic macrophages induce disruption of glutamate metabolism in periodontal ligament stem cells contributing to their compromised osteogenic potential.

Macrophage pyroptosis is of key importance to host defence against pathogen infections and may participate in the progression and recovery of periodontitis. However, the role of pyroptotic macrophages in regulating periodontal ligament stem cells (PDLSCs), the main cell source for periodontium renewal, remains unclear. First, we found that macrophage pyroptosis were enriched in gingiva tissues from periodontitis patients compared with those of healthy people through immunofluorescence.

NSUN5/TET2-directed chromatin-associated RNA modification of 5-methylcytosine to 5-hydroxymethylcytosine governs glioma immune evasion.

Malignant glioma exhibits immune evasion characterized by highly expressing the immune checkpoint CD47. RNA 5-methylcytosine(m5C) modification plays a pivotal role in tumor pathogenesis. However, the mechanism underlying m5C-modified RNA metabolism remains unclear, as does the contribution of m5C-modified RNA to the glioma immune microenvironment.

Dynamic Survival Risk Prognostic Model and Genomic Landscape for Atypical Teratoid/Rhabdoid Tumors: A Population-Based, Real-World Study.

Background: An atypical teratoid/rhabdoid tumor (AT/RT) is an uncommon and aggressive pediatric central nervous system neoplasm. However, a universal clinical consensus or reliable prognostic evaluation system for this malignancy is lacking. Our study aimed to develop a risk model based on comprehensive clinical data to assist in clinical decision-making.

Harnessing Mechanical Stress with Viscoelastic Biomaterials for Periodontal Ligament Regeneration.

The viscoelasticity of mechanically sensitive tissues such as periodontal ligaments (PDLs) is key in maintaining mechanical homeostasis. Unfortunately, PDLs easily lose viscoelasticity (e.g.

Integrated analysis of single-cell and bulk RNA sequencing data reveals the association between hypoxic tumor cells and exhausted T cells in predicting immune therapy response.

Continuous stimulation of tumor neoantigens and various cytokines in the tumor microenvironment leads to T cell dysfunction, but the specific mechanisms by which these key factors are distributed among different cell subpopulations and how they affect patient outcomes and treatment response are incompletely characterized. By integrating single-cell and bulk sequencing data of non-small cell lung cancer patients, we constructed a clinical outcome-associated T cell exhaustion signature. We discovered a significant association between the T cell exhaustion state and tumor cell hypoxia.

High expression of VTA1 is an adverse prognostic factor in lung adenocarcinoma.

Lung adenocarcinoma (LUAD) is a common pathological type of non-small cell lung cancer; identifying preferable biomarkers has become one of the current challenges. Given that VTA1 has been reported associated with tumor progression in various human solid cancers but rarely reported in LUAD, herein, RNA sequencing data from TCGA and GTEx were obtained for analysis of VTA1 expression and differentially expressed gene (DEG). Furthermore, functional enrichment analysis of VTA1-related DEGs was performed by GO/KEGG, GSEA, immune cell infiltration analysis, and protein-protein interaction (PPI) network.

Influence of Cuticular Waxes from Triticale on Rumen Fermentation: A Metabolomic and Microbiome Profiling Study.

Cuticular wax, a critical defense layer for plants, remains a relatively unexplored factor in rumen fermentation. We investigated the impact of cuticular wax on rumen fermentation using triticale as a model. In total, six wax classes were identified, including fatty acids, aldehydes, alkane, primary alcohol, alkyresorcinol, and β-diketone, with low-bloom lines predominated by 46.

Pharmacogenomic Analysis of Combined Therapies against Glioblastoma Based on Cell Markers from Single-Cell Sequencing.

Glioblastoma is the most common and aggressive form of primary brain cancer and the lack of viable treatment options has created an urgency to develop novel treatments. Personalized or predictive medicine is still in its infancy stage at present. This research aimed to discover biomarkers to inform disease progression and to develop personalized prophylactic and therapeutic strategies by combining state-of-the-art technologies such as single-cell RNA sequencing, systems pharmacology, and a polypharmacological approach.

Constructing an APOBEC-related gene signature with predictive value in the overall survival and therapeutic sensitivity in lung adenocarcinoma.

Background: APOBEC family play an important role in cancer mutagenesis and tumor development. The role of APOBEC family in lung adenocarcinoma (LUAD) has not been studied comprehensively.

Materials And Methods: The expression data of pan-cancer as well as LUAD was obtained from public databases.