Effects of application of on strawberry growth and rhizosphere microbial community structure in greenhouse.

D74 has good growth-promoting effects on a variety of crops. We investigated the growth-promoting effects of D74 on strawberry and rhizosphere microbial community under facility-cultivated condition. The results showed that: 1) D74 treatment significantly increased the dry and fresh weights of roots at the flowering stage by 40.

Biochemical characterization and molecular docking of a novel alkaline-stable keratinase from Amycolatopsis sp. BJA-103.

Amycolatopsis sp. BJA-103 was isolated for its exceptional feather-degradation capability, leading to the purification, cloning, and heterologous expression of the keratinase enzyme, KER0199. Sequence analysis places KER0199 within the S8 protease family, revealing <60 % sequence similarity to known proteases.

Metabolic crosstalk between roots and rhizosphere drives alfalfa decline under continuous cropping.

Considerable biological decline of continuously cropped alfalfa may be tightly linked to rhizosphere metabolism. However, plant-soil feedbacks and age-related metabolic changes in alfalfa stands remain unexplored. The aim of this study was to identify the linkages of rhizosphere and root metabolites, particularly autotoxins and prebiotics, to alfalfa decline under continuous cropping.

Harnessing biosynthesized selenium nanoparticles for recruitment of beneficial soil microbes to plant roots.

Root exudates can benefit plant growth and health by reshaping the rhizosphere microbiome. Whether nanoparticles biosynthesized by rhizosphere microbes play a similar role in plant microbiome manipulation remains enigmatic. Herein, we collect elemental selenium nanoparticles (SeNPs) from selenobacteria associated with maize roots.

Biological Decline of Alfalfa Is Accompanied by Negative Succession of Rhizosphere Soil Microbial Communities.

The growth and biological decline of alfalfa may be linked to the rhizosphere microbiome. However, plant-microbe interactions in the rhizosphere of alfalfa and associated microbial community variations with stand age remain elusive. This study explored the successional pattern of rhizosphere microbial communities across different aged alfalfa stands and its relationship with alfalfa decline.

Rhizosphere microbial ecological characteristics of strawberry root rot.

Introduction: Strawberry ( × ananassa Duch.) holds a preeminent position among small fruits globally due to its delectable fruits and significant economic value. However, strawberry cultivation is hampered by various plant diseases, hindering the sustainable development of the strawberry industry.

Widely targeted metabolomic, transcriptomic, and metagenomic profiling reveal microbe-plant-metabolic reprogramming patterns mediated by Streptomyces pactum Act12 enhance the fruit quality of Capsicum annuum L.

Plant growth-promoting rhizobacteria, such as Streptomyces pactum Act12, promote crop growth and stress resistance, but their contribution to fruit quality is still poorly understood. Herein we conducted a field experiment to ascertain the effects of S. pactum Act12-mediated metabolic reprogramming and underlying mechanisms in pepper (Capsicum annuum L.

Potential applications of microbial enhanced oil recovery to heavy oil.

Heavy oil accounts for around one-third of total global oil and gas resources. The progressive depletion of conventional energy reserves has led to an increased emphasis on the efficient exploitation of heavy oil and bitumen reserves in order to meet energy demand. Therefore, it is imperative to develop new technologies for heavy oil upgrading and recovery.

Biocontrol of Root Diseases and Growth Promotion of the Tuberous Plant Aconitum carmichaelii Induced by Actinomycetes Are Related to Shifts in the Rhizosphere Microbiota.

Soil Actinomycetes have been used as biocontrol agents against soil-borne plant diseases, yet little is known about their effects on the structure of the rhizosphere microbiota and the long-term effects on crop yield and disease intensity after the application of Actinomycetes is stopped. Here, we conducted 3-year plot experiments to investigate the roles of two Actinomycetes strains (Streptomyces pactum Act12 and Streptomyces rochei D74) in the biocontrol of soil-borne root diseases and growth promotion of monkhood (Aconitum carmichaelii). We also examined their long-term effects after soil application of a mixed Actinomycetes preparation (spore powder) was completed.

Saccharopolyspora qinghaiensis sp. nov., a novel actinobacterium isolated from a salt lake.

A novel halophilic, Gram-positive and aerobic actinobacterium, designated strain AFM 20147, was isolated from a sediment sample collected from Xiaochaidan Salt Lake of Qinghai, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AFM 20147 belongs to the genus Saccharopolyspora, shows high sequence similarities to Saccharopolyspora griseoalba AFM 10238 (99.41%) and Saccharopolyspora halophila YIM 90500 (98.

Role of Streptomyces pactum in phytoremediation of trace elements by Brassica juncea in mine polluted soils.

The industrial expansion, smelting, mining and agricultural practices have increased the release of toxic trace elements (TEs) in the environment and threaten living organisms. The microbe-assisted phytoremediation is environmentally safe and provide an effective approach to remediate TEs contaminated soils. A pot experiment was conducted to test the potential of an Actinomycete, subspecies Streptomyces pactum (Act12) along with medical stone compost (MSC) by growing Brassica juncea in smelter and mines polluted soils of Feng County (FC) and Tongguan (TG, China), respectively.

α-Glucosidase inhibitors and phytotoxins from Streptomyces xanthophaeus.

Twenty-four metabolites 1-24 were isolated from the fermentation broth of Streptomyces xanthophaeus. Their structures were elucidated on the basis of spectroscopic analysis and by comparison of their NMR data with literature data reported. Daidzein (1), genistein (2) and gliricidin (3) inhibited α-glucosidase in vitro with IC values of 174.

[Construction of SPA7074-deficient mutant of biocontrol strain Streptomyces pactum Act12 and characterization of its secondary metabolites].

Objective: To disrupt spa7074, which encodes a member of the TetR family transcriptional factors, in biocontrol strain Act12 and characterize the secondary metabolites in the mutant strain.

Methods: We disrupted the gene spa7074 by homologous recombination. The secondary metabolites of the mutant strain Δspa7074 and Act12 were detected by HPLC.

Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery.

Background: Lipopeptides are known as promising microbial surfactants and have been successfully used in enhancing oil recovery in extreme environmental conditions. A biosurfactant-producing strain, Bacillus atrophaeus 5-2a, was recently isolated from an oil-contaminated soil in the Ansai oilfield, Northwest China. In this study, we evaluated the crude oil removal efficiency of lipopeptide biosurfactants produced by B.

Gabosines P and Q, new carbasugars from Streptomyces sp. and their α-glucosidase inhibitory activity.

Two new polyoxygenated cyclohexenone 'ketocarbasugars', named gabosines P and Q (1 and 2), were isolated from the culture of the actinomycete Streptomycetes strain no. 8, along with two known cyclic dipeptides. The structures and absolute configurations of the new metabolites were determined by spectroscopic data (1D- and 2D-NMR, HR-ESI-MS, and IR), chemical transformation, and electronic circular dichroism (ECD).

Saccharopolyspora griseoalba sp. nov., a novel actinomycete isolated from the Dead Sea.

A novel halotolerant actinomycete, designated strain AFM 10238, was isolated from a sediment sample collected from the Dead Sea of Israel. The isolate grew at 15-45 °C, pH 6-12 and with 0-15 % (w/v) NaCl. Strain AFM 10238 contains meso-diaminopimelic acid as cell wall diamino acid, and galactose and arabinose as the whole cell sugars.

Drought-tolerant Streptomyces pactum Act12 assist phytoremediation of cadmium-contaminated soil by Amaranthus hypochondriacus: great potential application in arid/semi-arid areas.

Microbe-assisted phytoremediation provides an effective approach to clean up heavy metal-contaminated soils. However, severe drought may affect the function of microbes in arid/semi-arid areas. Streptomyces pactum Act12 is a drought-tolerant soil actinomycete strain isolated from an extreme environment on the Qinghai-Tibet Plateau, China.

Biodegradation of paraffin wax by crude Aspergillus enzyme preparations for potential use in removing paraffin deposits.

Paraffin deposition problems have plagued the oil industry. Whist mechanical and chemical methods are problematic, microbiological method of paraffin removal is considered an alternative. However, studies have mainly investigated the use of bacteria, with little attention to the potential of fungi.

Oligotrophy is Helpful for the Isolation of Bioactive Actinomycetes.

It is necessary to develop new methods for the isolation of unknown actinomycetes from soils. To evaluate the effects of oligotrophic medium on the isolation of soil actinomycetes and develop a new isolation method, the Gause's synthetic medium was diluted to one tenth the recommended concentration in the present study. Soil dilution plate technique was used to isolate actinomycetes from the soil samples.

Paenibacillus quercus sp. nov., isolated from rhizosphere of Quercus aliena var. acuteserrata.

A Gram-positive, spore-forming, rod-shaped and motile bacterium, designated strain 1-25(T), was isolated from the rhizosphere of Quercus aliena var. acuteserrata in Taibai Mountain, Shaanxi Province, China. 16S rRNA gene sequence analysis showed that strain 1-25(T) belongs to the genus Paenibacillus.

[Effects of actinomycetes agent on ginseng growth and rhizosphere soil microflora].

Taking the ginseng in Xiao Xing' an Mountains of Northeast China as test object, this paper studied the effects of applying Streptomyces pactum (Act12) on ginseng growth and on the soil microflora in root zone and root surface. After treated with Act12, the yield and quality of ginseng' s medicinal part improved, the induced enzyme activities in leaves and the root activity increased, and the numbers and proportions of soil bacteria and actinomycetes increased significantly while those of soil fungi decreased. Compared with the control, the soil microflora in treatment Act12 changed.

[Microecological mechanisms of red-leaf disease occurrence in Salvia miltiorrhiza Bge].

A comparative study was made on the nutrient content in rhizosphere soil and the microflora in rhizosphere soil and on rhizoplane of healthy and red-leaf diseased Salvia miltiorrhiza plants, aimed to approach the microecological mechanisms of red-leaf disease occurrence in S. miltiorrhiza. The N, P, K, and Mn contents in the diseased plant leaves were significantly lower than those in the healthy plant leaves (P < 0.

[Identification of two Fusarium isolates and their crude toxin allelopathic effect on Cucumis melo seedlings].

Eight fungi isolates were obtained from Fusarium-infected Cucumis melo (melon) plants and their rhizosphere soils. Taking melon cultivar 'Xitian 1' as test material, the re-inoculation and seed germination experiments were conducted to investigate the pathogenicity and growth inhibition effect of these fungi isolates on melon. Through the determination of the induced enzyme activities, resistant substance contents, and cell membrane permeability of potted melon roots, the allelopathic effect of the crude toxins of two harmful fungi was studied, and according to the morphological characteristics and Internal Transcribed Spacer (ITS) sequencing, the two harmful fungi were identified.

Bioactive alkaloids produced by Pseudomonas brassicacearum subsp. Neoaurantiaca, an endophytic bacterium from Salvia miltiorrhiza.

Eleven compounds were isolated from the culture of an endophytic bacterium Pseudomonas brassicacearum subsp. Neoaurantiaca in Salvia miltiorrhiza Bunge. Their structures were elucidated by spectroscopic methods as cyclo-(Gly-L-Ala) (1), cyclo-(L-Ala-L-Ala) (2), cyclo-(L-Pro-Gly) (3), cyclo-(L-Pro-L-Ser) (4), cyclo-(L-Ala-trans-4-hydroxy-L-Pro) (5), cyclo-(L-Val-L-Pro) (6), cyclo-(Gly-L-Tyr) (7), cyclo-(L-Ala-L-Tyr) (8), cyclo-(L-Tyr-trans-4-hydroxy-L-Pro) (9), 3-methylhydantoin (10) and 2-piperidinone (11).