Integrated analysis of m6A regulator mediated RNA methylation and key immune related genes in ischemic stroke.

Unlabelled: The role of RNA N6-methyladenosine (m6A) modifications in modulating the immune microenvironment during ischemic stroke (IS) pathogenesis remains poorly characterized. This investigation systematically explores m6A-mediated immune regulation in IS and identifies critical immune-related biomarkers. Transcriptomic profiles from 108 IS samples were analyzed to discern m6A regulatory patterns.

Seasonal Variation in In Hospite but Not Free-Living, Symbiodiniaceae Communities Around Hainan Island, China.

Coral reefs are increasingly threatened by global climate change, and mass bleaching and mortality events caused by elevated seawater temperature have led to coral loss worldwide. Hainan Island hosts extensive coral reef ecosystems in China, yet seasonal variation in Symbiodiniaceae communities within this region remains insufficiently understood. We aimed to investigate the temperature-driven adaptability regulation of the symbiotic Symbiodiniaceae community in reef-building corals, focusing on the environmental adaptive changes in its community structure in coral reefs between cold (23.

Radar-Based Cross-Domain Human Behavior Recognition Using Physics-Informed Data Augmentation and Multi-Source Domain Adversarial Neural Network.

Falling is a common but fatal human behavior in life. With the rapid growth of the aging population, fall-related human behavior recognition has been extensively investigated using radar. Nevertheless, human behavior recognition frequently exhibits suboptimal generalization capabilities due to the scarcity of labeled data.

Enhanced Bifunctional Electrocatalysis of VN via D-Band Engineering for Rechargeable Zn-Air Batteries.

The insufficient d-electron density in V metal atoms limits O dissociation, constraining the kinetics and activity of the oxygen reduction reaction and the oxygen evolution reaction in VN-based catalysts. Herein, Co dopant was employed to modulate the electronic characteristics of the d-orbitals and associated free energy of VN, thereby further enhancing its catalytic activity. XPS and XANES spectra revealed that Co atoms replaced V and strongly bonded with neighboring N atoms, effectively regulating the local electronic structure.

Network toxicology reveals collaborative mechanism of 4NQO and ethanol in esophageal squamous cell carcinogenesis.

Tobacco smoking and alcohol consumption are significant risk factors for esophageal squamous cell carcinoma (ESCC), with their Collaborative interaction amplifying carcinogenic risks. To elucidate the role and underlying mechanisms of tobacco-alcohol co-exposure in ESCC pathogenesis, we treated the immortalized human normal esophageal epithelial cell line NE-1 and C57BL/6 mice with 4-nitroquinoline-1-oxide (4NQO, a tobacco carcinogen analog) combined with ethanol. Results demonstrated that 4NQO-ethanol co-exposure accelerated esophageal squamous carcinogenesis.

Harnessing artificial intelligence for brain disease: advances in diagnosis, drug discovery, and closed-loop therapeutics.

Brain diseases pose a significant global health challenge due to their complexity and the limitations of traditional medical strategies. Recent advancements in artificial intelligence (AI), especially deep learning models like Convolutional Neural Networks (CNNs), Recurrent Neural Networks (RNNs), and Graph Neural Networks (GNNs), offer powerful new tools for analysis. These neural networks are effective at extracting complex patterns from high-dimensional data.

MYB20, an R2R3-type MYB transcription factor, negatively regulates salt and cold stress tolerance in pears.

As one of the largest families of transcription factors (TFs) in plants, MYB TFs play an important role in regulation responses to environmental stressors. However, the precise function of MYB TFs in abiotic stress responses in pear is not yet well-understood. Here, we identify an R2R3-type MYB TF, PpcMYB20, in pear that is significantly induced by multiple abiotic stresses, as evidenced by elevated expression after NaCl, cold, PEG6000, and abscisic acid (ABA) treatments.

Genomic insights into deleterious mutations and their impact on agronomic traits during pear domestication.

The pear ( spp.), a perennial fruit tree, is subjected to genetic alterations over decades or even centuries to adapt to complex climatic and cultivation conditions. Genome-wide studies of deleterious mutations remain limited in perennial fruit trees, particularly regarding the effects of domestication on deleterious mutations.

A Novel Injectable Composite Hydrogel HAp-GelMA Co-Doped Magnesium/Zinc Promotes Bone Generation and Angiogenesis Synergistically.

Bone is a crucial organ in the human body; however, conventional bone repair materials, such as autologous and allogeneic bone, are associated with challenges like limited availability and infection risks. Therefore, the development of novel biomaterials for bone repair is essential. This study employed a hydrothermal method to synthesize hydroxyapatite (HAp) doped with various elements and characterized its physicochemical properties.

EPHA4 signaling dysregulation links abnormal locomotion and the development of idiopathic scoliosis.

Idiopathic scoliosis (IS) is the most common form of spinal deformity with unclear pathogenesis. In this study, we first reanalyzed the loci associated with IS, drawing upon previous studies. Subsequently, we mapped these loci to candidate genes using either location-based or function-based strategies.

Two novel phages infecting isolated from the epipelagic ocean.

, an aerobic anoxygenic photoheterotrophic bacterial genus, plays a vital role in carbon and energy cycling in marine environments. However, their phage predators remain poorly understood, with only two strains previously reported. This study isolated and characterized a novel phage, vB_EauS-R34L1 (R34L1), and its sub-strain vB_EauS-R34L2 (R34L2), from coastal seawater.

Application of responsive nano-drug carriers in bone tumor chemotherapy.

Bone tumors, especially malignant ones, have high mortality and disability rates, and traditional treatment methods have limitations. Responsive nano-drug carriers achieve targeted delivery and controlled release of drugs by precisely responding to the tumor microenvironment or external stimuli, thereby improving drug efficacy and reducing toxic side effects. These carriers, including liposomes, micelles, and nano-gels, can respond to internal stimuli such as pH, redox, enzymes, and hypoxia, as well as external stimuli such as light, magnetic fields, and ultrasound.

Mitochondrial metabolic rescue in post-COVID-19 syndrome: MR spectroscopy insights and precision nutritional therapeutics.

Post-COVID-19 Condition (PCC), impacting 30-90% of survivors, is characterized by persistent fatigue and metabolic dysfunction, often linked to underlying mitochondrial impairment. This review examines current evidence on mitochondrial-targeted nutrition therapies, with a focus on magnetic resonance spectroscopy (MRS) as a tool for assessing metabolic recovery. Key findings highlight reduced adenosine triphosphate (ATP) production, heightened oxidative stress, and disrupted mitochondrial biogenesis- metabolic abnormalities that closely mirror those seen in chronic fatigue syndromes.

Research Progress in Medical Biomaterials for Bone Infections.

Bone infection is a debilitating condition characterized by inflammation of the bone and its marrow. It poses significant challenges in clinical practice due to its recalcitrant nature and difficulty in eradicating the infecting microorganisms. Recent advancements in the field of medical biomaterials have shown hope in the treatment of bone infections.

Nanomaterials reshape the pulmonary mechanical microenvironment: novel therapeutic strategies for respiratory diseases.

Respiratory diseases, including chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and lung cancer, exhibit elevated death rates and pathological intricacy, requiring advancements that surpass the constraints of traditional therapies. This study comprehensively outlines the novel applications of nanomaterials in respiratory medicine by accurately modulating the pulmonary mechanical microenvironment, encompassing alveolar surface tension, extracellular matrix rigidity, and the immune-fibroblast interaction network. The precise delivery, stimuli-responsive characteristics, and biomimetic design of nanomaterials markedly improve drug concentration at the lesion site and mitigate fibrosis, inflammation, and malignant tumor advancement by disrupting mechanical signaling pathways.

Spatiotemporal distribution, composition, and influencing factors of lipophilic marine algal toxins in the Beibu Gulf, China: Implications for necessity of offshore seawater monitoring.

Lipophilic marine algal toxins (LMATs) are persistent, bioaccumulative metabolites that pose significant threats to marine biodiversity and seafood safety. To comprehensively understand LMATs pollution in offshore natural fishing grounds, we investigated the spatiotemporal distribution and composition of 13 LMATs in surface seawater in Beibu Gulf's offshore area, along with their key influencing factors and latitudinal variations across coastal regions in China. We detected 20-methyl spirolide G (SPX20G) for the first time in coastal waters in China, alongside other frequently detected LMATs, including okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-2 (PTX2), azaspiracid-1 (AZA1), azaspiracid-2 (AZA2), gymnodimine (GYM), and 13-desmethyl spirolide C (SPX1).

NPC1L1 on pancreatic adenocarcinoma cell functions as a two-pronged checkpoint against antitumor activity.

Pancreatic adenocarcinoma (PAAD) is a highly lethal malignancy with an immunosuppressive microenvironment and a limited immunotherapy response Cholesterol is necessary for rapid growth of cancer cells, and cholesterol metabolism reprogramming is a hallmark of PAAD. How PAAD cells initiate cholesterol reprogramming to sustain their growth demand and suppressive immunomicroenvironment remains elusive. In this study, we for the first time revealed that PAAD cells overcome cholesterol shortage and immune surveillance via ectopically overexpressing NPC1L1, a cholesterol transporter but function as a two-pronged checkpoint, which not only directly suppresses TCR activation of CD8T cells but also hijacks the intracellular cholesterol from CD8T cells.

Impact of COVID-19 on door-to-wire time in ST-segment elevation myocardial infarction treatment: the role of digital communication.

Introduction: ST-segment elevation myocardial infarction (STEMI) is a life-threatening cardiovascular emergency necessitating rapid reperfusion. During the COVID-19 pandemic, healthcare providers faced the challenge of ensuring timely STEMI interventions while managing the risk of viral transmission in hospitals. This study aims to analyze changes in the door-to-wire (D-to-W) time for STEMI treatment across three pandemic phases-early pre-epidemic phase (Group C), initial lockdown phase (Group A), and intermediate normalization phase (Group B).

Tunable Hydro/Dicarboxylation of Ethylene with CO via a Barrierless Radiolytic Free-Radicals Pathway.

The hydro/dicarboxylation of ethylene (CH) feedstock with CO to produce high-value carboxylic acids is an industrially relevant yet challenging reaction due to their extremely low reactivities. Herein, we present an effective strategy to synthesize propionic acid and succinic acid from a mixture of CO and CH catalyzed by high-energy water radiolysis. The process involves the generation of CO radical anions via the barrierless attachment of hydrated electrons to CO, facilitating an ambient, continuous carboxylation of CH with an efficiency of 81.

Lipid metabolic reprogramming and associated ferroptosis in osteosarcoma: From molecular mechanisms to potential targets.

Osteosarcoma is a common bone tumor in adolescents, which is characterized by lipid metabolism disorders and plays a key role in tumorigenesis and disease progression. Ferroptosis is an iron-dependent form of programmed cell death associated with lipid peroxidation. This review provides an in-depth analysis of the complex relationship between lipid metabolic reprogramming and associated ferroptosis in OS from the perspective of metabolic enzymes and metabolites.

A soft, ultra-tough and multifunctional artificial muscle for volumetric muscle loss treatment.

The escalating prevalence of skeletal muscle disorders highlights the critical need for innovative treatments for severe injuries such as volumetric muscle loss. Traditional treatments, such as autologous transplants, are constrained by limited availability and current scaffolds often fail to meet complex clinical needs. This study introduces a new approach to volumetric muscle loss treatment by using a shape-memory polymer (SMP) based on block copolymers of perfluoropolyether and polycaprolactone diol.

Case report: Microsatellite instability-high pancreas adenosquamous carcinoma with postoperative liver metastasis recurrence treated with multimodality therapy achieving complete pathological response.

Pancreatic adenosquamous carcinoma (PASC) is a rare subtype of pancreatic cancer (PC), with no established consensus on the optimal treatment for postoperative liver metastasis recurrence. We report a case of a 48-year-old male patient who underwent radical surgery and was pathologically diagnosed with microsatellite instability-high (MSI-H) PASC. The patient experienced liver metastasis recurrence following single-agent gemcitabine adjuvant chemotherapy.

Machine learning algorithms for the early detection of bloodstream infection in children with osteoarticular infections.

Background: Bloodstream infection (BSI) poses a significant life-threatening risk in pediatric patients with osteoarticular infections. Timely identification of BSI is crucial for effective management and improved patient outcomes. This study aimed to develop a machine learning (ML) model for the early identification of BSI in children with osteoarticular infections.

Multiomics identification of ALDH9A1 as a crucial immunoregulatory molecule involved in calcific aortic valve disease.

Mitochondrial dysfunction and immune cell infiltration play crucial yet incompletely understood roles in the pathogenesis of calcific aortic valve disease (CAVD). This study aimed to identify immune-related mitochondrial genes critical to the pathological process of CAVD using multiomics approaches. The CIBERSORT algorithm was employed to evaluate immune cell infiltration characteristics in CAVD patients.