Targeting the mPGES-PGE-EP4 pathway with MF63, MK886, and Grapiprant as a potential therapeutic strategy for Escherichia coli-induced endometritis in dairy cows.

Escherichia coli (E. coli) is the primary causative agent of endometritis in dairy cows. Antibiotics and non-steroidal anti-inflammatory drugs (NSAIDs) are the main treatments; however, prolonged antibiotic use promotes bacterial resistance, while NSAIDs can delay ovulation and impair reproduction.

Exogenous prostaglandin D as a modulator in bovine endometritis: implications for reducing antibiotic use in dairy cattle.

Introduction: Bovine endometritis is a common postpartum uterine infection that significantly impacts the health and production performance of dairy cows, leading to economic losses for farms. Bovine endometritis is closely associated with pathogenic microorganisms, disturbances in uterine microecology, and localized inflammatory damage. () is the primary pathogenic bacterium responsible for bovine endometritis.

Investigating the role of the mPGES-PGE₂-EP4 pathway in -induced mastitis in dairy cows: insights for non-antibiotic therapeutic strategies.

() is the primary causative agent of bovine mastitis. Currently, antibiotic therapy remains the cornerstone of mastitis treatment; necessitating the identification of alternative therapeutic options. This study employed cultured bovine bone marrow-derived macrophages (BMDMs) to systematically assess the potential of microsomal prostaglandin e synthase-1 (mPGES-1) inhibitors (MF63, MK886) and EP4 receptor inhibitor (Grapiprant) in modulating inflammatory responses and reducing tissue damage.

A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples.

Conventional laboratory methods for handling valuable biological samples typically use pipettes or needles, which are prone to issues such as dead volume and sample waste. Furthermore, the sampling and processing of pathogenic bacteria, such as () in environmental wastewater, require labor-intensive procedures with multiple complex steps. To overcome these limitations, we developed a pipette integrated with a microfluidic chip for bacteria sampling and delivery.

A microfluidic system for rapid enrichment and sensitive detection of E. coli based on bilayer membrane and high flux droplets.

Rapid and sensitive detection of Escherichia coli (E. coli) is crucial for ensuring water safety. Traditional culture-based detection technologies are time-consuming, cumbersome, and difficult to meet immediate needs, while existing instrument-based detection is costly, time-consuming, and struggle to process complex samples.

Dual roles of the TLR2/TLR4/NLRP3-H-PGDS-PGD axis in regulating the inflammatory response in Escherichia coli-infected bovine bone marrow-derived macrophages and endometrial tissue.

Endometritis in dairy cows is associated with pathogenic microorganisms, local inflammatory injuries, and uterine microecological disorders. Escherichia coli (E. coli) is the primary pathogen responsible for bovine endometritis onset; however, the underlying pathomechanisms remain unclear.

Mechanisms of action of the proline hydroxylase-adenosine pathway in regulating apoptosis and reducing myocardial ischemia-reperfusion injury.

The aim of this study is to explore the protective mechanism of proline hydroxylase (PHD) in reducing myocardial ischemia-reperfusion injury (MIRI) through the hypoxia-inducible factor (HIF)-1α-adenosine-MAPK/ERK signaling pathway, with the goal of identifying potential drug targets and therapeutic strategies for the clinical management of MIRI. A rat model of MIRI was established using 45 male Sprague-Dawley (SD) rats, which were randomly divided into the following three groups: sham operation (n = 15), MIRI model (n = 15), and MIRI + FG-4592 preconditioning (n = 15) groups. Cardiac function was assessed by echocardiographic measurements of the left ventricular end-diastolic diameter (LVIDd), left ventricular contractile diameter (LVIDs), left ventricular shortening fraction (FS), and left ventricular ejection fraction (EF).

An efficient, high-throughput enrichment system for the rapid detection of E. coli at low concentrations in water.

Certain virulent strains of Escherichia coli (E. coli), notably the enterohemorrhagic serotype O157:H7, are recognized for causing diarrhea, gastroenteritis, and a range of illnesses that pose significant risks to public health and the safety of drinking water supplies. Early detection and management of E.

Preliminary exploration of metabolomics mechanisms in patients with patent foramen ovale and migraine.

This study aimed to investigate the metabolic mechanisms underlying the combination of patent foramen ovale (PFO) and migraine by assessing metabolite expression before and after interventional occlusion surgery. The study included 11 PFO patients from the Heart Center of Xinjiang Medical University Affiliated Hospital of Traditional Chinese Medicine, who underwent transcatheter PFO intervention and occlusion surgery between January 2018 and February 2023, and 11 healthy controls. Blood samples were collected pre-surgery, 3 days post-surgery, and 30 days post-surgery for metabolomics analysis.

A Large-field droplets for high-throughput Escherichia coli identification within one field of view.

Droplet microfluidic chips have emerged as an efficient platform for single-cell analysis due to their high sensitivity, efficiency, and throughput, showing significant potential in pathogen detection. However, current droplet microfluidic chips encounter challenges in large-scale droplet quantification and precise imaging, rendering them unsuitable for the high-throughput pathogen detection required for a large number of samples. To address these issues, this study developed a high-precision fluorescence imaging system utilizing a confocal reflective fluorescence approach, which is an advanced microscopy technique that combines confocal microscopy and reflected fluorescence imaging.

Lane Attribute Classification Based on Fine-Grained Description.

As an indispensable part of the vehicle environment perception task, road traffic marking detection plays a vital role in correctly understanding the current traffic situation. However, the existing traffic marking detection algorithms still have some limitations. Taking lane detection as an example, the current detection methods mainly focus on the location information detection of lane lines, and they only judge the overall attribute of each detected lane line instance, thus lacking more fine-grained dynamic detection of lane line attributes.

Targeting inhibits lung adenocarcinoma cell proliferation and progression by interacting with Y-box binding protein 1.

Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality worldwide. Increasing evidence suggests that long noncoding RNAs play crucial roles in lung cancer pathogenesis. We previously identified a novel lncRNA, , which is associated with tumor cell proliferation.

Biosynthesis and Biotechnological Synthesis of Hydroxytyrosol.

Hydroxytyrosol (HT), a plant-derived phenolic compound, is recognized for its potent antioxidant capabilities alongside a spectrum of pharmacological benefits, including anti-inflammatory, anti-cancer, anti-bacterial, and anti-viral properties. These attributes have propelled HT into the spotlight as a premier nutraceutical and food additive, heralding a new era in health and wellness applications. Traditional methods for HT production, encompassing physico-chemical techniques and plant extraction, are increasingly being supplanted by biotechnological approaches.

High lymphocyte signature genes expression in parathyroid endocrine cells and its downregulation linked to tumorigenesis.

Background: To date, because of the difficulty in obtaining normal parathyroid gland samples in human or in animal models, our understanding of this last-discovered organ remains limited.

Methods: In the present study, we performed a single-cell transcriptome analysis of six normal parathyroid and eight parathyroid adenoma samples using 10 × Genomics platform.

Findings: We have provided a detailed expression atlas of parathyroid endocrine cells.

Effect of Exogenous and Endogenous Ectoine on Development, Metabolism, and Pigment Stability.

, a key player in fermented food production, is known for generating pigments (MPs) and monacolin K (MK), possessing bioactive properties. However, the limited stability of MPs and mycotoxin citrinin (CTN) constrain the industry. Extremolytes like ectoine, derived from bacteria, exhibit cytoprotective potential.

The Effect and Mechanism of POSTN and Its Alternative Splicing on the Apoptosis of Myocardial Cells in Acute Myocardial Infarction: A Study in Vitro.

Our study aimed to investigate key molecular targets in the pathogenesis of AMI, and provide new strategy for the treatment. In this work, the myocardial ischemia and hypoxia model was constructed by using HL-1 mouse cardiomyocytes. The over-expressing POSTN wild-type, mutant and negative control lentiviruses (GV492-POSTNWT,GV492-POSTN-MUT, GV492-NC) was conducted and transfected.

Histone Acetyltransferase Rtt109 Regulates Development, Morphogenesis, and Citrinin Biosynthesis in .

Histone acetyltransferase (HAT) has been reported to be pivotal for various physiological processes in many fungi. However, the functions that HAT Rtt109 perform in edible fungi and the underlying mechanism remains unclear. Here, we identified the gene in , constructed the knockout strain (Δ) and its complementary strain (Δ:com) by CRISPR/Cas9 methods, and functionally characterized the roles that Rtt109 play in .

Syringic Acid Alleviates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating Gut Microbiota.

Inflammatory bowel disease is known to be associated with alterations in gut microbiota. The bioactive compound syringic acid has been shown to alleviate inflammatory bowel disease, but its interaction with gut microbiota and mechanism of action remain unclear. To address this, we conducted a study in which we investigated the potential benefits of syringic acid in a mouse model of dextran sulfate sodium-induced colitis through gut microbiota modulation.

A Novel Microbial Consortia Catalysis Strategy for the Production of Hydroxytyrosol from Tyrosine.

Hydroxytyrosol, a valuable plant-derived phenolic compound, is increasingly produced from microbial fermentation. However, the promiscuity of the key enzyme HpaBC, the two-component flavin-dependent monooxygenase from often leads to low yields. To address this limitation, we developed a novel strategy utilizing microbial consortia catalysis for hydroxytyrosol production.

[Long-term intermittent fasting induces abnormal lipid accumulation in mouse liver].

Short-term intermittent fasting (IF) is beneficial to weight control in patients with nonalcoholic fatty liver disease, but the impact of long-term IF is not clear. In this study, healthy C57BL/6N mice with 4-month alternate day fasting (ADF) were used to study the effects of long-term IF on systemic and liver lipid metabolism. The results showed that, compared with the Ad Libitum group, the weight and food conversion rate of mice in the ADF group were markedly decreased and increased respectively, and the liver index and the liver content of triglyceride were significantly increased by pathological examination.

Preconceptional exposure of adult male rats to bisphenol S impairs insulin sensitivity and glucose tolerance in their male offspring.

Since the use of bisphenol A (BPA) has been restricted because of its endocrine disruptor properties, bisphenol S (BPS) has been widely used as a substitute of BPA. However, BPS exerts similar effects on metabolic health as BPA. The effects of maternal exposure to BPA and BPS on the metabolic health of offspring have been largely documented during the past decade.

Candidate genes and their alternative splicing may be potential biomarkers of acute myocardial infarction: a study of mouse model.

Background: Acute myocardial infarction (AMI) is one of the leading causes of death in human being, and an effective diagnostic biomarker is still lacking. Whilst some gene association with AMI has been identified by RNA sequencing (RNA-seq), the relationship between alternative splicing and AMI is not clear.

Methods: We retrieved myocardial tissues within 24 h from mice with induced AMI and sham, and analysed the differentially expressed genes (DEGs) and differential alternative splicing genes (DASGs) by RNA-seq.