A serum TNFR2-based model effectively predicates preoperative microvascular invasion and stratifies the tumor recurrence risk in hepatocellular carcinoma.

Aim: Microvascular invasion (MVI) is a key risk factor for hepatocellular carcinoma (HCC) recurrence. There is a lack of methods to diagnose MVI preoperatively. The objective of this study was to develop a model for preoperative prediction of MVI in HCC.

Diagnostic value of CD20 combined with CD138 positive expression in patients with chronic endometritis.

Background: This study aims to evaluate the diagnostic value of CD138 and CD20 immunohistochemical staining in chronic endometritis (CE).

Methods: A total of 131 patients with reproductive needs, treated at the Second Affiliated Hospital of Fujian Medical University between August 2020 and July 2021, were enrolled. Patients were divided into a CD138-positive group (n=91) and a CD138-negative group (n=40).

Targeted Lung Premetastasis Niche: Mechanisms, Strategies, and Application.

Lung metastasis remains a leading cause of cancer mortality, driven not only by primary tumors but crucially by dynamic interactions within the premetastatic niche (PMN). Emerging evidence reveals that organ-specific PMN formation precedes tumor cell arrival, creating an immunosuppressive microenvironment conducive to metastatic colonization. Primary tumor-derived factors interact with lung resident cells, promoting the recruitment of bone marrow-derived cells and creating a favorable microenvironment for the migrating tumor cells.

Growth differentiation factor 7 alleviates the proliferation and metastasis of hepatocellular carcinoma.

Background And Aims: Inflammatory factors play significant roles in the development and occurrence of hepatocellular carcinoma (HCC). However, the tumor-protective functions of growth differentiation factors (GDFs) in HCC are yet to be clarified. In this study, we aimed to evaluate the expression levels of 10 GDFs in tumor and paratumor tissues from patients with HCC and perform and experiments to elucidate the role of GDF7 in regulating the proliferation and metastasis of HCC.

Associations of dietary total, heme, non-heme iron intake with diabetes, CVD, and all-cause mortality in men and women with diabetes.

Background: Iron metabolism disorders significantly increase the risk of diabetes and its related complications by inducing oxidative stress, inflammation, insulin resistance, and disturbances in glucose and lipid metabolism. However, whether dietary iron intake can influence progression of diabetes remains unclear. The present study aims to investigate the relationship between total iron, heme iron, and non-heme iron intake and diabetes, CVD, and all-cause mortality among men and women with diabetes in the U.

A Janus hydrogel that enables wet tissue adhesion and resists abdominal adhesions.

Hydrogels have indeed achieved significant advancements, yet their clinical translation has been hampered by their inherent limitations in wet adhesion properties. Furthermore, the design of adhesive hydrogels that can resist postoperative adhesions remains an intricate challenge. In this study, we introduce a Janus hydrogel (JGP) that offers a novel approach to address these challenges.

CCL16 inhibits tumor proliferation and metastasis in HCC by impacting CK19 phenotype.

Background And Aims: Cytokeratin 19-positive (CK19+) hepatocellular carcinoma (HCC) is an aggressive subtype with poor outcomes. The initiation and development of CK19+ HCC in the background of liver cirrhosis remains unclear. This study investigated the role of the cirrhosis-related gene C-C motif chemokine ligand 16 (CCL16) in the development of CK19+ HCC.

Underwater Highly Pressure-Sensitive Fabric Based on Electric-Induced Alignment of Graphene.

Wearable pressure sensors have received widespread attention owing to their potential applications in areas such as medical diagnosis and human-computer interaction. However, current sensors cannot adapt to extreme environments (e.g.

3D Zero Poisson's Ratio Honeycomb Structure for Morphing Wing Applications.

Such as flying creatures, morphing aircraft can expand their aerodynamic flight envelopes by changing aerodynamic shapes, significantly improving the scope of application and flight efficiency. A novel 3D Zero Poisson's Ratio (ZPR) honeycomb structure is designed to meet the flexible deformation requirements of morphing aircraft. The 3D ZPR honeycomb can deform in the three principal directions with smooth borders and isotropic.

METTL3-mediated m6A mRNA contributes to the resistance of carbon-ion radiotherapy in non-small-cell lung cancer.

Lung cancer is one of the leading causes of death among cancer patients worldwide. Carbon-ion radiotherapy is a radical nonsurgical treatment with high local control rates and no serious adverse events. N6-methyladenosine (m6A) modification is one of the most common chemical modifications in eukaryotic messenger RNA (mRNA) and has important effects on the stability, splicing, and translation of mRNAs.

Activation of Gonadotropin-releasing Hormone Receptor Impedes the Immunosuppressive Activity of Decidual Regulatory T Cells via Deactivating the Mechanistic Target of Rapamycin Signaling.

Understanding maternal immune tolerance is crucial for the development of therapeutics for immunological pregnancy complications. Decidual regulatory T cells (Tregs) play a pivotal role in the maintenance of maternal immune tolerance. Using a murine allogeneic pregnancy model in the current study, we identified the up-regulation of gonadotropin-releasing hormone receptor (GnRHR) in decidual T cell subsets including CD4 conventional T cells, CD8 T cells, and CD4Foxp3 Tregs.

Deciphering extrachromosomal circular DNA in Arabidopsis.

Extrachromosomal circular DNA (eccDNA) is independent of the chromosome and exists in many eukaryotes. However, the nature and origin of eccDNA in plants remains unclear. In this study, we sequenced 12 samples from four tissues (leaf, flower, stem and root) with three biological replicates.

Inhibition of leucine-rich repeats and calponin homology domain containing 1 accelerates microglia-mediated neuroinflammation in a rat traumatic spinal cord injury model.

Background: Spinal cord injury (SCI) triggers the primary mechanical injury and secondary inflammation-mediated injury. Neuroinflammation-mediated insult causes secondary and extensive neurological damage after SCI. Microglia play a pivotal role in the initiation and progression of post-SCI neuroinflammation.

Spatio-temporal analysis of socio-economic characteristics for pulmonary tuberculosis in Sichuan province of China, 2006-2015.

Background: The disease burden caused by pulmonary tuberculosis (TB) in Sichuan province still persisted at a high level, and large spatial variances were presented across regional distribution disparities. The socio-economic factors were suspected to affect the population of TB notification, we aimed to describe TB case notification rate (CNR) and identify which factors influence TB epidemic are necessary for the prevention and control of the disease in Sichuan province.

Methods: A retrospective cross-sectional study and an ecological spatial analysis was conducted to quantify the presence and location of spatial clusters of TB by the Moran's I index and examined these patterns with socio-economic risk factors by hierarchical Bayesian spatio-temporal model.

Traditional three-dimensional printing technology versus three-dimensional printing mirror model technology in the treatment of isolated acetabular fractures: a retrospective analysis.

Objective: This study was performed to compare the clinical outcomes of traditional three-dimensional (3D) printing technology and 3D printing mirror model technology in the treatment of isolated acetabular fractures.

Methods: Prospectively maintained databases were reviewed to retrospectively compare patients with an isolated acetabular fracture who were treated with traditional 3D printing technology (Group T) or 3D printing mirror model technology (Group M) from 2011 to 2017. In total, 146 advanced-age patients (146 hips) with an isolated acetabular fracture (Group T, n = 72; Group M, n = 74) were assessed for a mean follow-up period of 29 months (range, 24-34 months).

Plasma level manifesting as an indicator in progression of breast cancer.

To investigate whether plasma level could be an indicator in clinical progression of breast cancer. Plasma level of expression was detected by quantitative real time PCR and the level of c-myc protein in breast cancer tissues was detected by immunohistochemistry. The expression level of mRNA in supernatant of cancer cells culture was measured compared with the nonbreast cancer cells.

Research advancements in the neurological presentation of flaviviruses.

Owing to the large-scale epidemic of Zika virus disease and its association with microcephaly, properties that allow flaviviruses to cause nervous system diseases are an important area of investigation. At present, although potential pathogenic mechanisms of flaviviruses in the nervous system have been examined, they have not been completely elucidated. In this paper, we review the possible mechanisms of blood-brain barrier penetration, the pathological effects on neurons, and the association between virus mutations and neurotoxicity.

N-acetyltransferases from three different organisms displaying distinct selectivity toward hexosamines and N-terminal amine of peptides.

N-acetyltransferases are a family of enzymes that catalyze the transfer of the acetyl moiety (COCH) from acetyl coenzyme A (Acetyl-CoA) to a primary amine of acceptor substrates from small molecules such as aminoglycoside to macromolecules of various proteins. In this study, the substrate selectivity of three N-acetyltransferases falling into different phylogenetic groups was probed against a series of hexosamines and synthetic peptides. GlmA from Clostridium acetobutylicum and RmNag from Rhizomucor miehei, which have been defined as glucosamine N-acetyltransferases, were herein demonstrated to be also capable of acetylating the free amino group on the very first glycine residue of peptide in spite of varied catalytic efficiency.

A novel enzymatic method for synthesis of glycopeptides carrying natural eukaryotic N-glycans.

A novel enzymatic approach was developed for facile production of glycopeptides carrying natural eukaryotic N-glycans. In this approach, peptides can be GlcNAcylated at one or two natural N-glycosylation sites via two-step enzymatic reactions catalyzed by an evolved N-glycosyltransferase (ApNGT) and a glucosamine N-acetyltransferase (GlmA), respectively. The resulting GlcNAc-peptides were further modified by an endo-β-N-acetylglucosaminidase M mutant (EndoM) to generate glycopeptides.

Production of homogeneous glycoprotein with multisite modifications by an engineered -glycosyltransferase mutant.

Naturally occurring -glycoproteins exhibit glycoform heterogeneity with respect to -glycan sequon occupancy (macroheterogeneity) and glycan structure (microheterogeneity). However, access to well-defined glycoproteins is always important for both basic research and therapeutic purposes. As a result, there has been a substantial effort to identify and understand the catalytic properties of -glycosyltransferases, enzymes that install the first glycan on the protein chain.

Histone deacetylase inhibitor, Romidepsin (FK228) inhibits endometrial cancer cell growth through augmentation of p53-p21 pathway.

Objective: Romidepsin (FK228), a Histone Deacetylase (HDAC) inhibitor, has been used for anti-cancer therapies. However, the anti-cancer effect of FK228 and its underlying mechanism in endometrial carcinoma (EC) have not been studied. The aime of this study was to investigate the anti-cancer effects of FK228 and the associated mechanism(s) in EC.

Tuberculosis burden in China: a high prevalence of pulmonary tuberculosis in household contacts with and without symptoms.

Background: In the context of decreasing tuberculosis prevalence in China, we examined the effectiveness of screening household contacts of tuberculosis patients.

Methods: A tuberculosis survey was conducted in 2008. All 3,355 household contacts of notified tuberculosis cases were examined with a questionnaire interview, chest X-ray and three sputum smear tests.

[Safety and immunological effect of domestic split influenza virus vaccine].

Objective: To evaluate the safety and immunological effect of domestic split influenza virus vaccine.

Methods: All 606 subjects were divided into three groups by under 6, 16-60 and above 60 years old. Each age group was divided as study group (n = 213), control group 1 (n = 195) and control group 2 (n= 198) by Table of Random Number, one domestic vaccine and two imported vaccines were respectively inoculated in three group people.