Ultrasound-guided selective supraclavicular nerve block combined with modified clavipectoral fascial plane block for midshaft clavicule fracture surgery: a case report.

Background: Conventional anesthesia for clavicular fracture surgery poses significant challenges. General anesthesia risks pulmonary complications, while traditional nerve blocks may cause diaphragmatic paralysis or motor impairment. Ultrasound-guided techniques improve precision, but standalone superficial cervical plexus or interscalene brachial plexus blocks often fail to fully anesthetize the deep clavicular region, especially in polytrauma patients.

A solid AIE sensor for visual monitoring of food freshness.

In this work, we developed a fluorescence probe HCPM with AIE effect. The probe HCPM in solid state showed obvious fluorescence colormetric response from orange-red to green for cadaverine vapor with high selectivity and could quantitative detect cadaverine vapor and a visual detection platform was constructed by combining it with a smartphone. Also, the probe HCPM exhibited significant fluorescence colormetric responses for 12 BAs and could detect the different concentrations of cadaverine in EtOH/HO (1/9, v/v, pH = 7.

Genome-wide analysis of the TCP transcription factor family in mung bean and its dynamic regulatory network under salt stress.

The TCP gene family encodes plant-specific transcription factors that regulate plant growth, development, and stress responses. Although this gene family has been widely studied in various species, its function in mung bean () remains unclear. In this study, we identified 26 VrTCP genes, which were classified into two groups: Class I (PCF subfamily) and Class II (CYC/TB1 and CIN subfamilies).

Sensor with Strong Solid Emission for Fluorescence Colormetric Detection of 1, 4-Dioxane in Water and Vapor Based on the Keto-Enol Isomerisation.

In this work, an easy prepared solid fluorescent sensor 3-(benzo[d]thiazol-2-yl)-1-hydroxy-4-oxo-3,4-dihydrophthalazine-6-carboxylic acid (BPCA) was designed and synthesized and the structure was proved by IR, UV-Vis, NMR, HRMS and elemental analysis, which displayed high selectivity and sensitivity for fluorescence colormetric from green to blue sensing 1, 4-dioxane in water, and the detection limit was obtained 0.009% and the stability constant was 7.4 × 10 M.

Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis.

Bacteriocytes are specialized insect cells adapted to harbor symbionts. However, their low number in individual whiteflies makes obtaining enough for transcriptome sequencing challenging. Here, we present a protocol for the isolation of whitefly bacteriocytes.

Telomere-to-telomere, gap-free genome of mung bean () provides insights into domestication under structural variation.

Mung bean (), an essential annual legume, holds substantial value in global agriculture due to its short growth cycle, low input requirements, and nutritional benefits. Despite extensive domestication, the genetic mechanisms underlying its morphological and physiological evolution remain incompletely understood. In this study, we present a gap-free, telomere-to-telomere genome assembly of the mung bean cultivar 'Weilv-9', achieved through the integration of PacBio HiFi, Oxford Nanopore, and high-throughput chromosome conformation capture (Hi-C) sequencing technologies.

Analysis of non-tuberculous mycobacteria types in high tuberculosis endemic areas.

Background: The incidence of non-tuberculous mycobacteria (NTM) has been rising globally, posing significant challenges for diagnosis and treatment, particularly in regions with high tuberculosis (TB) incidence. This study aims to investigate the epidemiological, demographic, and clinical characteristics of non-tuberculous mycobacterial lung disease (NTM-PD) in areas with a high incidence of TB.

Method: This study was conducted at the Affiliated Hospital of Zunyi Medical University from January 2017 to December 2021.

[Correlation Analysis of Serum Complement Level and Prognosis in Diagnosis of Aggressive Non-Hodgkin Lymphoma].

Objective: To explore the relationship between serum complement levels at diagnosis and prognosis in patients with aggressive non-Hodgkin lymphoma(NHL).

Methods: The clinical data of 102 patients with aggressive non-Hodgkin lymphoma diagnosed in the First Hospital of Lanzhou University from February 2017 to March 2023 were selected to analyze the correlation between serum complement C3 and C4 levels and prognosis of patients with aggressive NHL at the time of initial diagnosis. The optimal cut-off point of C3 and C4 were obtained by calculating the Jorden index through the receiver operating characteristic（ROC） curve, and 102 patients were divided into low C3 group (C3< 1.

Transcriptome profiling across 11 different tissues in Pisum sativum.

Objectives: Peas (Pisum sativum) are vital for human nutrition and have significantly contributed to the understanding of Mendelian inheritance laws. In this study, we performed transcriptome sequencing on 11 distinct pea tissues, offering an extensive gene expression dataset. This study not only provides valuable insights into the gene expression patterns across various pea tissues but also lays the foundation for future research aimed at understanding the molecular mechanisms underlying pea growth, development, and response to environmental stimuli.

An AIE probe for simultaneous monitoring of endogenous and exogenous hypochlorite and Zn at dual channels in living cells.

Zn and ClO may be associated with a variety of pathologies, and their simultaneous measurement is crucial for disease diagnosis and environmental protection. In this work, we synthesized an independent AIE probe, HNTE, through a one step reaction. The probe HNTE displayed a distinctive fluorescence color change from deep yellow to blue for ClO and to green for Zn.

An AIE probe for the fluorescence and colorimetric sensing of La.

In this work, a fluorescent probe with aggregation-induced emission effect was synthesized by grafting naphtho[2,3-]furan-1,3-dione and 2-hydrazinylbenzo[]thiazole. The probe could recognize La selectively and sensitively accompanied with an obvious fluorescence and color change from green to blue. Moreover, with the help of AIE properties, probe N achieved the detection of La in the solid state.

Development of an AIE-active fluorescent probe for the simultaneous detection of Al and viscosity and imaging in Alzheimer's disease model.

Alzheimer's disease is associated both with imbalances in Al production and changes in viscosity in cells. Their simultaneous measurement could therefore provide valuable insights into Alzheimer's disease pathology. Their simultaneous measurement would therefore be of great value in investigating the pathological mechanism of Alzheimer's disease.

Risk factors associated with intraoperative persistent hypotension in pancreaticoduodenectomy.

Background: Intraoperative persistent hypotension (IPH) during pancreaticoduodenectomy (PD) is linked to adverse postoperative outcomes, yet its risk factors remain unclear.

Aim: To clarify the risk factors associated with IPH during PD, ensuring patient safety in the perioperative period.

Methods: A retrospective analysis of patient records from January 2018 to December 2022 at the First Affiliated Hospital of Nanjing Medical University identified factors associated with IPH in PD.

Throat microbiota drives alterations in pulmonary alveolar microbiota in patients with septic ARDS.

Objectives: The translocation of intestinal flora has been linked to the colonization of diverse and heavy lower respiratory flora in patients with septic ARDS, and is considered a critical prognostic factor for patients.

Methods: On the first and third days of ICU admission, BALF, throat swab, and anal swab were collected, resulting in a total of 288 samples. These samples were analyzed using 16S rRNA analysis and the traceability analysis of new generation technology.

Chemical Flexibility of Atomically Precise Metal Clusters.

Ligand-protected metal clusters possess hybrid properties that seamlessly combine an inorganic core with an organic ligand shell, imparting them exceptional chemical flexibility and unlocking remarkable application potential in diverse fields. Leveraging chemical flexibility to expand the library of available materials and stimulate the development of new functionalities is becoming an increasingly pressing requirement. This Review focuses on the origin of chemical flexibility from the structural analysis, including intra-cluster bonding, inter-cluster interactions, cluster-environments interactions, metal-to-ligand ratios, and thermodynamic effects.

Pigmentiphaga kullae CHJ604 improved the growth of tobacco by degrading allelochemicals and xenobiotics in continuous cropping obstacles.

Plant autotoxicity is considered to be one of the important causes of continuous cropping obstacles in modern agriculture, which accumulates a lot of allelochemicals and xenobiotics and is difficult to solve effectively. To overcome tobacco continuous obstacles, a strain Pigmentiphaga kullae CHJ604 isolated from the environment can effectively degrade these compounds in this study. CHJ604 strain can degrade 11 types of autotoxicity allelochemicals and xenobiotics (1646.

A novel AIE material for sensing of Erythromycin in pure water by hydrogen bond in portable test strips and cellular imaging applications.

Erythromycin could be used to treat various bacterial infection, but it was harmful to the colonic microflora. Therefore, it is highly desirable to develop a fluorescence probe that could selectively and sensitively detect Erythromycin in pure water. In this work, a fluorescent probe named EHMC, which exhibited aggregation-induced emission (AIE) characteristic in solid state and water/EtOH binary solvent was developed for "turn on" sensing Erythromycin in pure water with high selectivity and sensitivity (detection limit: 1.

Circularly polarized luminescence enlargement from crystals to oriented films of enantiopure 2D hybrid perovskites.

Chiral 2D organic-inorganic hybrid perovskites (C-2D-OIHPs) with circularly polarized luminescence (CPL) have important promising applications in optical, electronic, and chiroptoelectronic devices. Herein, we report enantiomeric crystals of /-FMBA)PbBr. (FMBA = 4-fluorophenethylamine), which demonstrated bright room-temperature CPL emission.

Deafness-Associated ADGRV1 Mutation Impairs USH2A Stability through Improper Phosphorylation of WHRN and WDSUB1 Recruitment.

The ankle-link complex (ALC) consists of USH2A, WHRN, PDZD7, and ADGRV1 and plays an important role in hair cell development. At present, its architectural organization and signaling role remain unclear. By establishing Adgrv1 Y6236fsX1 mutant mice as a model of the deafness-associated human Y6244fsX1 mutation, the authors show here that the Y6236fsX1 mutation disrupts the interaction between adhesion G protein-coupled receptor V subfamily member 1 (ADGRV1) and other ALC components, resulting in stereocilia disorganization and mechanoelectrical transduction (MET) deficits.

Inhibiting 5-hydroxytryptamine receptor 3 alleviates pathological changes of a mouse model of Alzheimer's disease.

Extracellular amyloid beta (Aβ) plaques are main pathological feature of Alzheimer's disease. However, the specific type of neurons that produce Aβ peptides in the initial stage of Alzheimer's disease are unknown. In this study, we found that 5-hydroxytryptamin receptor 3A subunit (HTR3A) was highly expressed in the brain tissue of transgenic amyloid precursor protein and presenilin-1 mice (an Alzheimer's disease model) and patients with Alzheimer's disease.

Lymphocyte-C-reactive protein ratio can differentiate disease severity of COVID-19 patients and serve as an assistant screening tool for hospital and ICU admission.

In this study, we aimed to explore whether lymphocyte-C-reactive protein ratio (LCR) can differentiate disease severity of coronavirus disease 2019 (COVID-19) patients and its value as an assistant screening tool for admission to hospital and intensive care unit (ICU). A total of 184 adult COVID-19 patients from the COVID-19 Treatment Center in Heilongjiang Province at the First Affiliated Hospital of Harbin Medical University between January 2020 and March 2021 were included in this study. Patients were divided into asymptomatic infection group, mild group, moderate group, severe group, and critical group according to the Diagnosis and Treatment of New Coronavirus Pneumonia (ninth edition).

Bacteriocyte development is sexually differentiated in Bemesia tabaci.

Some symbiotic microbes are restricted to specialized host cells called bacteriocytes. However, the molecular and cellular mechanisms underlying the development of bacteriocytes are largely obscure. We find that maternally inherited bacteriocytes proliferate in adult females but degenerate in adult males of the whitefly Bemisia tabaci.