Uncovering key biomarkers, potential therapeutic targets and development of deep learning model in heart failure.

Heart failure (HF) represents a significant public health concern, characterized by elevated rates of mortality and morbidity. Recent advancements in gene sequencing technologies have led to the identification of numerous genes associated with heart failure. By utilizing available gene expression data from the Gene Expression Omnibus (GEO) database, we conducted a screening for differentially expressed genes (DEGs) related to heart failure.

Decoupling aggregation in dense protein solutions: Structural reconfiguration through disulfide reduction weakens nanoscale adhesion forces.

High-concentration protein solutions are indispensable in food engineering for their nutritional density, yet excessive viscosity hinders processing efficiency and compromises textural quality. A green and scalable strategy is proposed for reducing the viscosity of high-concentration cod protein (CPs) solutions through structural reengineering driven by reductive disulfide bond cleavage. NaSO selectively disrupts disulfide bonds, inducing conformational unfolding to expose buried hydrophobic domains.

Controlled aggregation of whey protein toward the exceptional tolerance of high ionic strength upon heating.

Ready-to-drink whey protein beverages featuring electrolyte replenishment and whey protein intake are experiencing increasing popularity in the market for sports nutrition. However, such preference was challenged by NaCl-promoted thermotropic gelation. In this study, whey protein aggregates (WPA) with improved tolerance to NaCl-promoted thermal aggregation were successfully fabricated by regulating pH and protein concentration during preheating modification.

Umami-Transformer: A deep learning framework for high-precision prediction and experimental validation of umami peptides.

In food field, both identification of umami peptides and their sensory evaluation are limited by low efficiency of traditional methods and subjectivity of human-based assessments. To overcome these issues, Umami-Transformer was developed by integrating Transformer architecture with eight physicochemical descriptors. A classification accuracy of 0.

Isolation, identification and comparative genomic analysis of from Mongolian horse vagina.

Introduction: Reproductive health in mares is pivotal for the sustainability of the equine industry, yet vaginal microbiota dysbiosis remains an underrecognized contributor to infections such as endometritis and bacterial vaginosis. While spp. dominate healthy vaginal ecosystems in humans and livestock, their role in equine reproductive health, particularly in resilient breeds like Mongolian mares, is poorly understood.

Effects of polysaccharides and proteins from Tricholoma matsutake on gelatinization, rheology, structural properties, and in vitro digestibility of rice flour.

The growing demand for low-glycemic-index functional foods motivates the exploration of natural starch modifiers that balance digestibility and physicochemical performance. This study investigated the effects of Tricholoma matsutake polysaccharides (TMP) and proteins (TMPN) on the gelatinization, rheology, structural properties, and in vitro digestibility of rice flour (RF). TMP and TMPN 5 % (w/w) were incorporated into RF, their impacts were analyzed using rapid viscosity analysis (RVA), dynamic rheology, differential scanning calorimetry (DSC), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), in vitro digestion assays, and α-amylase inhibition assays.

From Aggregation-Prone to Stabilized Whey Protein: Deciphering the Role of Disulfide Bonds and Individual Proteins in Thermal Resistance Ability.

Whey protein (WP) has been gaining popularity in high-protein beverages due to its nutritional advantages. However, its heat-induced aggregation behavior, particularly in salt-containing systems, remains a key barrier to commercialization. Previously, modified whey protein (MWP) with thermostability in the presence of NaCl was synthesized through controlled aggregation.

Identifying emphysema risk using brominated flame retardants exposure: a machine learning predictive model based on the SHAP methodology.

Background: Emphysema is a major contributor to lung disease progression and is associated with significant health risks, including exacerbations, mortality, and lung cancer. While environmental exposures, such as brominated flame retardants (BFRs), have been suggested as risk factors, their role in emphysema prediction has been largely overlooked. This study aimed to develop a machine learning (ML) model to predict emphysema risk incorporating BFRs exposure data and demographic characteristics.

Advanced strategies for the processing and utilization of star Anise (Illicium verum hook. F.): blanching, drying and bioactive compounds extraction.

Star anise, a globally popular spice, is subjected to blanching followed by drying to enhance its shelf life and prepare it for essential oil extraction. The essential oil derived from star anise is rich in several important bioactive compounds, particularly shikimic acid. The techniques employed in process such as blanching, drying, and extraction significantly influence the content and bioactivity of the resulting extracts.

Effects of Matsutake-Derived Insoluble Fiber on the Pasting Properties, Structural Characteristics, and In Vitro Digestibility of Rice Flour.

This study explores the effects of -derived insoluble dietary fiber (TMIDF) on the pasting behavior, structural properties, and in vitro digestibility of rice flour. The incorporation of 5% TMIDF significantly increased the peak viscosity (from 2573.21 to 2814.

A novel strategy based on mouse organoid biosensor for detecting umami substances and their synergistic effect.

Conventional analysis systems face challenges in quantifying the umami perception and synergy within complex food matrices, especially mimicking human taste discrimination. We thereby developed an umami biosensor based on taste bud organoids and flow cytometry (TOS-FC), which exhibits a broad dynamic range (10 to 2 × 10 M for monosodium glutamate (MSG)), strong linear correlation (R > 98 %), and stable operability beyond sensor lifespan. The TOS-FC platform successfully quantified three representative umami stimuli-MSG, inosine 5'-monophosphate (IMP), and glutamic acid-proline dipeptide (EP, a representative umami peptide).

ZmSSRP1, transactivated by OPAQUE11, positively regulates starch biosynthesis in maize endosperm.

Starch synthesis is crucial for crop yield and quality. This study reveals an O11-ZmSSRP1 module of kernel starch biosynthesis in maize (Zea mays). We identify STARCH SYNTHESIS REGULATING PROTEIN 1 (ZmSSRP1) positively regulates amylose and amylopectin-dependent starch synthesis in maize endosperm.

Comparing the kangduo surgical robot-01 and da vinci xi system for endometrial cancer surgery: a multi-center, randomized, parallel-controlled, noninferiority trial.

Background: The outcomes of patients who undergo surgical staging for endometrial cancer using the KangDuo Surgical Robot-01 (KD-SR-01) have not been compared with those of patients who undergo this procedure using the da Vinci Xi system. The aim of this study is to evaluate the efficacy and safety of the KD-SR-01 system for surgical staging of endometrial cancer by comparing short-term outcomes to those of counterparts who underwent surgery using the da Vinci Xi system.

Materials And Methods: This multi-center, randomized, noninferiority trial was conducted at four hospitals.

Probiotic Potential of M6 Isolated from Equines and Its Alleviating Effect on DSS-Induced Colitis in Mice.

Colitis in equines has high morbidity and mortality rates, which severely affects the development of the equine-breeding industry. With the issuance of antibiotic bans, there is an urgent need for healthier and more effective alternatives. In recent years, probiotics have been widely used as microbial feed additives in animal husbandry, playing a crucial role in preventing and treating diarrhea and regulating host immune function.

Associations of biological aging with the morbidity and all-cause mortality of patients with lung cancer.

Accelerated biological aging has been suggested to be an important risk factor for age-related diseases, but its role in the development of lung cancer is still unclear. The aim of this study was to analyze the relationship between biological aging and lung cancer and its impact on mortality using the NHANES database. We calculated the chronological age-adjusted biological age of 40,765 participants in the U.

Carboxymethyl-β-cyclodextrin grafted chitosan embedded oyster (Crassostrea gigas) ferritin can alleviate lead-induced liver injury by oral administration.

Ferritin is expected to alleviate lead-induced oxidative damage. Despite being a relatively stable macromolecular protein that can withstand high temperatures and extreme pH conditions without its structure and activity being compromised, oral administration of ferritin may still result in its degradation within the gastrointestinal tract, reducing its bioavailability. In recent years, microcapsules have emerged as an effective approach in oral protein delivery to enhance bioavailability.

Directional control of the formation rate, and structural and mechanical properties of yuba films via disulfide bond- or ionic interaction-mediated soy protein aggregation.

Background: Yuba is a worldwide popular soybean product, but its development is currently challenged by the time-consuming fabrication process.

Results: In this study, we proposed a simple yet effective strategy to enhance the formation rate and tensile strength of yuba films by introducing calcium ions and sodium metabisulfite (SM). Results show that calcium ion increased the density and continuity of the yuba films, while SM led to the formation of larger protein particles.

A Novel Cystatin Gene from Sea Cucumber (): Characterization and Comparative Expression with Cathepsin L During Early Stage of Hypoxic Exposure-Induced Autolysis.

Autolysis in sea cucumber has long been a threat to raw material storage and product processing. The involvement of endogenous cysteine protease in sea cucumber autolysis has been proved extendedly. However, as an essential part of the mechanism of autolysis, the role of its endogenous inhibitor has seldom been reported.

Artificial Intelligence in CT Angiography for the Detection of Coronary Artery Stenosis and Calcified Plaque: A Systematic Review and Meta-analysis.

Purpose: We aimed to evaluate the diagnostic performance of artificial intelligence (AI) in detecting coronary artery stenosis and calcified plaque on CT angiography (CTA), comparing its diagnostic performance with that of radiologists.

Methods: A thorough search of the literature was performed using PubMed, Web of Science, and Embase, focusing on studies published until October 2024. Studies were included if they evaluated AI models in detecting coronary artery stenosis and calcified plaque on CTA.

β-Cyclodextrin/resistant dextrin induced disparate gelling behaviors of high-protein liquid systems.

High-protein, high-fiber foods are in the spotlight because of their health properties. Due to aggregation during heat treatment when dietary fiber is added, the amount of soy protein that can be used in the formulation of high-protein, high-fiber foods is limited. The effects of two types of soluble dietary fibers, β-cyclodextrin (β-CD) and resistant dextrin (RD), on the tunable thermal stability of soy proteins (SPs) and preheat-modified soy protein particles (SPPs), as well as the underlying mechanisms, were investigated.

Comparison of three extraction methods, DMU-SPME, SAFE, and SDE, for the analysis of flavor compounds in soy sauce.

The dual mode unity solid-phase microextraction (DMU-SPME) was evaluated against traditional solvent-based methods, including solvent-assisted flavor evaporation (SAFE) and simultaneous distillation extraction (SDE), to explore its potential as an alternative. Compared to SAFE and SDE, DMU-SPME is a green extraction technology for volatile organic compounds. The results demonstrated that DMU-SPME significantly outperformed both SAFE and SDE in extracting medium-volatility compounds (retention time of 20-40 min), identifying 55 unique volatiles.

Microalgae in health care and functional foods: β-glucan applications, innovations in drug delivery and synthetic biology.

Microalgae are emerging as a key player in healthcare, functional foods, and sustainable biotech due to their capacity to produce bioactive compounds like β-glucans, omega-3 fatty acids, and antioxidants in an eco-friendly manner. This review comprehensively discusses the role of microalgae in healthcare and functional foods, focusing particularly on β-glucan therapeutics, drug delivery innovations, and synthetic biology applications. In healthcare, microalgae-derived compounds show immense promise for treating diseases, boosting immunity, and tackling oxidative stress.

Rennet-assisted modification for enhanced freeze-thaw stability in sodium caseinate-stabilized high internal phase emulsions.

Poor freeze-thaw stability seriously limits the application of Pickering emulsions in the frozen food industry. This study developed sodium caseinate-stabilized high internal phase emulsions (NaCas-HIPEs) with enhanced freeze-thaw stability through rennet modification. The freeze-thaw stability and 3D printing properties of NaCas-HIPEs significantly improved as rennet addition increased from 0 % to 0.

Soluble nano-sized aggregates of Alaska pollock proteins engineered by the refolding process of pH-shifting.

Alaska pollock proteins (PPs) are aquatic proteins with a compact structure, which leads to poor solubility and limited application in food industries. This study explored the structural assembly and gelation behavior of the PP-soluble aggregates prepared by the refolding processing of acidic (pH 1.5-7) or alkaline (pH 12-7) pH-shifting treatment.

Impact of Cooking Processes on Volatile Flavor Compounds and Free Amino Acids in Fish Sauce.

Fish sauce is a widely used condiment in cooking. However, the effects of various cooking processes on its quality remain poorly understood. This study evaluated the quality of fish sauce subjected to various cooking methods (boiling for 10, 30, and 60 min and stir-frying for 10, 30, and 60 s) using sensory evaluation, electronic nose, gas chromatography-mass spectrometry, and free amino acid analysis.