mbSparse: an autoencoder-based imputation method to address sparsity in microbiome data.

The involvement of gut microbiota in host physiological activities is crucial, yet the high sparsity of microbiome data, marked by numerous zeros in count matrices, presents huge analytical challenges. To overcome this, we developed mbSparse, an imputation algorithm that leverages deep learning rather than traditional predefined count distributions. Utilizing a feature autoencoder for learning sample representations and a conditional variational autoencoder (CVAE) for data reconstruction, mbSparse effectively integrates these processes to enhance imputation.

Implications of Activity of Daily Living for Clinical Care of Older Adults Undergoing Urological Surgery-A Cross-Sectional Study.

Aim: This study aims to evaluate the clinical effectiveness of nursing classification based on Activities of Daily Living (ADL) scores to improve the quality of nursing care.

Design: A cross-sectional study.

Methods: This study was conducted from April 2023 to April 2024, involving a total of 570 older urological patients.

Ruxolitinib suppresses tumor growth in PTEN-deficient glioblastoma by inhibiting the STAT3-PDL1 axis-mediated the M2 polarization of macrophages.

Background: Glioblastoma (GBM) is the most malignant form of brain tumor, and GBM patients with poorer prognosis and highly immunosuppressive tumor microenvironment (TME) often exhibit PTEN deficiency in their tumor tissues. Therefore, new therapeutic strategies targeting immunosuppressive TME maybe useful in PTEN-deficient GBM.

Methods: Bioinformatics was used to assess gene expression, survival time and immunoinfiltration in PTEN-deficient GBM.

Utilizing Corneal Fluorescein and Conjunctival Lissamine Green Staining Combined with in vivo Confocal Microscopy for Grading the Severity of Dry Eye Disease.

Objective: To explore the application of corneal fluorescein sodium (CFS)-conjunctival lissamine green (CLG) staining combined with in vivo confocal microscopy (IVCM) in clinical grading of the severity of dry eye disease (DED).

Methods: Eleven normal persons (4 males and 7 females) and thirty-two mild, moderate, and severe DED patients (aged 22 to 56 years, and mean age 45.4 ± 12.

DFT mechanistic insights into the formation of the metal-dioxygen complex [Co(12-TMC)O] using HO as an [O] unit source.

The reaction of [M(L)] with HO as an [O] unit source and NEt as a base is a widely used biomimetic transition metal-peroxo and -superoxo complex [M(L)O] synthesis method, but the mechanism and accurate stoichiometry of the synthesis remain elusive. In this study, we performed DFT calculations to deeply understand the mechanism, using the synthesis of the cobalt-peroxo complex [Co(12-TMC)O] (12-TMC = (1,4,7,10-tetramethyl-1,4,7,10-tetraazacyclododecane)) from the reaction of [Co(12-TMC)] and HO in the presence of NEt as an example. The study found that cobalt-peroxo complex formation proceeds three stages: (Stage I) the conversion of [Co(12-TMC)] and HO to [Co(12-TMC)OH] and OOH˙ radical, (Stage II) the coordination of OOH˙ to [Co(12-TMC)] to give [Co(12-TMC)OOH], followed by deprotonation with NEt, affording [Co(12-TMC)O], and (Stage III) the transformation of [Co(12-TMC)OH] which is generated in Stage I to [Co(12-TMC)O].

Analysis of the Mechanism Underlying Radiotherapy Resistance Caused by Oligodendroglia Cells in Glioblastoma by Applying the Single-cell RNA Sequencing Technology.

Background: Glioblastoma (GBM) is an aggressive malignancy. The inherent resistance of GBM to radiotherapy poses great challenges for clinical treatment.

Objectives: The primary objective of this study is to explore the molecular mechanisms of radiotherapy resistance in GBM and identify the key influencing factors that contribute to this phenomenon.

Identifying and validating necroptosis-associated features to predict clinical outcome and immunotherapy response in patients with glioblastoma.

Background: Necroptosis is a type of programmed cell death involved in the pathogenesis of cancers. This work developed a prognostic glioblastoma (GBM) model based on necroptosis-related genes.

Methods: RNA-Seq data were collected from the TCGA database.

Role of NADPH Oxidase 4 on Dry Eye Syndrome in Mice.

This study aims to investigate the effect of NADPH oxidase 4 (NOX4)-mediated inflammation on concanavalin A (ConA)-induced dry eye syndrome (DES) in mice. Thirty-six mice were randomly divided into Control, Model, no-load Control, and NOX4 interference group. Adenovirus was injected (10 μL) into the lacrimal glands of both eyes of mice in no-load Control group and NOX4 interference group.

Enhanced Reactivities of Iron(IV)-Oxo Porphyrin Species in Oxidation Reactions Promoted by Intramolecular Hydrogen-Bonding.

High-valent iron-oxo species are one of the common intermediates in both biological and biomimetic catalytic oxidation reactions. Recently, hydrogen-bonding (H-bonding) has been proved to be critical in determining the selectivity and reactivity. However, few examples have been established for mechanistic insights into the H-bonding effect.

Wearable Sensor-Based Residual Multifeature Fusion Shrinkage Networks for Human Activity Recognition.

Human activity recognition (HAR) based on wearable sensors has emerged as a low-cost key-enabling technology for applications such as human-computer interaction and healthcare. In wearable sensor-based HAR, deep learning is desired for extracting human active features. Due to the spatiotemporal dynamic of human activity, a special deep learning network for recognizing the temporal continuous activities of humans is required to improve the recognition accuracy for supporting advanced HAR applications.

Expression of TXLNA in brain gliomas and its clinical significance: a bioinformatics analysis.

Background: To analyze the expression of TXLNA in brain gliomas and its clinical significance.

Methods: Gene Expression Profiling Interactive Analysis（GEPIA）and Chinese Glioma Genome Atlas（CGGA）databases were retrieved as the methods. To assess the disparity between TXLNA expression in glioma and normal brain tissue.

Proteomic analysis of anti-aging effects of Dendrobium nobile Lindl. alkaloids in aging-accelerated SAMP8 mice.

Senescence-accelerated mouse prone 8 (SAMP8) mice exhibit cognitive defects and neuron loss with aging, and were used to study anti-aging effects of Dendrobium nobile alkaloids (DNLA). DNLA (20 and 40 mg/kg) were orally administered to SAMP8 mice from 6 to 10 months of age. At 10-month of age, behavioral tests via Y-maze and Open-field and neuron damage via Nissl staining were evaluated.

Prediction of drug-target interactions via neural tangent kernel extraction feature matrix factorization model.

Drug discovery is a complex and lengthy process that often requires years of research and development. Therefore, drug research and development require a lot of investment and resource support, as well as professional knowledge, technology, skills, and other elements. Predicting of drug-target interactions (DTIs) is an important part of drug development.

Photo/Ni dual-catalyzed radical defluorinative sulfonylation to synthesize -difluoro allylsulfones.

Radical defluorinative functionalization of α-trifluoromethyl styrenes represents an effective way toward -difluoroalkenes. There are general interests in developing novel synthetic protocols for defluorinative functionalization with various types of radicals. However, reports on the preparation of -difluoro allylsulfones an -centered radical pathway are limited.

Prophylactic IVC filter placement in patients with severe intracranial, spinal cord, and orthopedic injuries at high thromboembolic event risk: A utilization and outcomes analysis of the National Trauma Data Bank.

Purpose: To determine relationships between prophylactic inferior vena cava filter (IVCF) insertion and pulmonary embolism (PE), deep venous thrombosis (DVT), and in-hospital mortality outcomes in patients with severe traumatic pelvic/lower extremity, intracranial, and spinal cord injuries.

Methods: Adult patients with severe traumatic pelvic/lower extremity, intracranial, and spinal cord injuries admitted to level I-IV trauma centers were selected from the National Trauma Data Bank (NTDB). IVCFs that were inserted both ≤48 h after admission and before a lower extremity venous ultrasound were defined as prophylactic.

Comprehensive analysis: Necroptosis-related lncRNAs can effectively predict the prognosis of glioma patients.

Glioma is the most common and fatal primary brain tumor in humans. A significant role for long non-coding RNA (lncRNA) in glioma is the regulation of gene expression and chromatin recombination, and immunotherapy is a promising cancer treatment. Therefore, it is necessary to identify necroptosis-related lncRNAs in glioma.

New biomarker: the gene HLA-DRA associated with low-grade glioma prognosis.

Background: Low-grade gliomas (LGG) are WHO grade II tumors presenting as the most common primary malignant brain tumors in adults. Currently, LGG treatment involves either or a combination of surgery, radiation therapy, and chemotherapy. Despite the knowledge of constitutive genetic risk factors contributing to gliomas, the role of single genes as diagnostic and prognostic biomarkers is limited.

Utilization and Comparative Effectiveness of Uterine Artery Embolization versus Hysterectomy for Severe Postpartum Hemorrhage: A National Inpatient Sample Study.

Purpose: To identify differences in mortality or length of hospital stay for mothers treated with uterine artery embolization (UAE) or hysterectomy for severe postpartum hemorrhage (PPH), as well as to analyze whether geographic or clinical determinants affected the type of therapy received.

Materials And Methods: This National Inpatient Sample study from 2005 to 2017 included all patients with live-birth deliveries. Severe PPH was defined as PPH that required transfusion, hysterectomy, or UAE.

Enthalpy-Entropy Compensation Effect in Oxidation Reactions by Manganese(IV)-Oxo Porphyrins and Nonheme Iron(IV)-Oxo Models.

"Enthalpy-Entropy Compensation Effect" (EECE) is ubiquitous in chemical reactions; however, such an EECE has been rarely explored in biomimetic oxidation reactions. In this study, six manganese(IV)-oxo complexes bearing electron-rich and -deficient porphyrins are synthesized and investigated in various oxidation reactions, such as hydrogen atom transfer (HAT), oxygen atom transfer (OAT), and electron-transfer (ET) reactions. First, all of the six Mn(IV)-oxo porphyrins are highly reactive in the HAT, OAT, and ET reactions.

Identifying Intermediates in Electrocatalytic Water Oxidation with a Manganese Corrole Complex.

Water nucleophilic attack (WNA) on high-valent terminal Mn-oxo species is proposed for O-O bond formation in natural and artificial water oxidation. Herein, we report an electrocatalytic water oxidation reaction with Mn tris(pentafluorophenyl)corrole () in propylene carbonate (PC). O was generated at the Mn potential with hydroxide, but a more anodic potential was required to evolve O with only water.

Predictors of Successful Yttrium-90 Radioembolization Bridging or Downstaging in Patients with Hepatocellular Carcinoma.

Purpose: This study aims to identify clinical and imaging prognosticators associated with the successful bridging or downstaging to liver transplantation (LT) in patients undergoing Yttrium-90 radioembolization (Y90-RE) for hepatocellular carcinoma (HCC).

Methods: Retrospectively, patients with Y90-RE naïve HCC who were candidates or potential candidates for LT and underwent Y90-RE were included. Patients were then divided into favorable (maintained or achieved Milan criteria (MC) eligibility) or unfavorable (lost eligibility or unchanged MC ineligibility) cohorts based on changes to their MC eligibility after Y90-RE.

SLC15A4 Serves as a Novel Prognostic Biomarker and Target for Lung Adenocarcinoma.

Background: SLC15A family members are known as electrogenic transporters that take up peptides into cells through the proton-motive force. Accumulating evidence indicates that aberrant expression of SLC15A family members may play crucial roles in tumorigenesis and tumor progression in various cancers, as they participate in tumor metabolism. However, the exact prognostic role of each member of the SLC15A family in human lung cancer has not yet been elucidated.

Recent Advances in Predicting Protein S-Nitrosylation Sites.

Protein S-nitrosylation (SNO) is a process of covalent modification of nitric oxide (NO) and its derivatives and cysteine residues. SNO plays an essential role in reversible posttranslational modifications of proteins. The accurate prediction of SNO sites is crucial in revealing a certain biological mechanism of NO regulation and related drug development.

Diagnostic and prognostic role of NR3C4 in breast cancer through a genomic network understanding.

The androgen receptor (AR, NR3C4) is believed to participate in the development of breast cancer, but its molecular mechanism and role in prognosis is still controversial and opaque. This study aimed to explore the expression, associations with clinicopathologic features and underlying molecular mechanisms of AR in breast cancer. The present study investigated invasive breast carcinoma through comprehensive bioinformatics.