Establishment and validation of an liver model based extracellular matrix for hepatotoxicity prediction.

IntroductionThree-dimension (3D) cell culture presents a promising alternative of drug-induced liver injury (DILI). To advance preclinical toxicology research, we developed an liver model using HepG2 for toxic evaluation.MethodsThe model was constructed based on the extracellular matrix.

Reversible Thermochromic Organosilicon Fibers Based on TiO@AgI Composites: Preparation, Properties, and Potential Applications.

The application of reversible thermochromic fibers (RTFs) is expanding from the smart wearable field toward industrial temperature detection and safety warning; however, most RTFs suffer from low color change temperature thresholds and poor thermal stability, which restrict their applications in medium and high-temperature places (>100 °C). Currently, overheating of equipment is a key factor that causes safety accidents, and the development of reliable visualized temperature warning materials is of great practical significance for accident prevention. To address these issues, TiO@AgI is blended with a ternary organosilicon oil spinning solution via a high-temperature air-induced crosslinking method to obtain a reversible thermochromic organosilicon fiber (TSiF) suitable for use in medium and high-temperature environments owing to its thermal stability.

The rice AT-rich pincer-like element family of conserved noncoding sequences regulates chromatin loop formation.

The comprehensive annotation of regulatory elements in linear genomes is needed to elucidate the molecular mechanisms underlying chromatin loop formation in plants. Here, we characterized a novel family of conserved noncoding sequences (CNSs) in the rice (Oryza sativa) genome. These sequences, known as AT-rich pincer-like elements (APEs), are composed of 13-bp repeat unit arrays in a reverse-forward configuration.

Clinical characteristics and genomic epidemiological survey of tuberculosis in Wuzhou, China, 2022.

Tuberculosis (TB) is a serious respiratory disease posing significant public health threats, such as the variation in () lineages and their associated drug resistance across regions. In 2022, clinical data and culture-positive TB samples were collected from the Third People's Hospital in Wuzhou, China. drug resistance and lineage were analyzed using whole-genome sequencing, while logistic regression was applied to identify factors influencing patient outcomes.

Development of a disease diagnostic model to predict the occurrence of central precocious puberty of female.

Objectives: To develop a clinical model for predicting the occurrence of Central Precocious Puberty based on the breast development outcomes in chinese girls.

Methods: This is a retrospective study, which included a total of 1,001 girls aged 6-9 years old who visited the outpatient clinic of Beijing Children's Hospital from January 2017 to October 2022 for "breast development". Participants were categorized into pubertal development (PD) cohort and simple premature breast development (PT) according to the criteria, and information was collected and tested for relevant indicators.

Flexible, breathable, and durable superhydrophobic cotton fabric modified by behenic acid, tung oil, and ZIF-8 with anti-icing and self-cleaning properties.

Textiles with self-cleaning and anti-icing capabilities in cold climates are essential for outdoor workers and enthusiasts. Superhydrophobic modification of textile surfaces is effective in imparting these characteristics. Although there are numerous methods available for manufacturing superhydrophobic textiles, careful consideration is warranted for environmental concerns over fluorochemicals, stability of superhydrophobic coatings, and fabric breathability.

Identification and expression analysis of XIP gene family members in rice.

Xylanase inhibitor proteins (XIP) are widely distributed in the plant kingdom, and also exist in rice. However, a systematic bioinformatics analysis of this gene family in rice (OsXIP) has not been conducted to date. In this study, we identified 32 members of the OsXIP gene family and analyzed their physicochemical properties, chromosomal localization, gene structure, protein structure, expression profiles, and interaction networks.

Enhancement of pyranoanthocyanin formation in blueberry wine with non-Saccharomyces yeasts.

The development of blueberry wine provides an alternative method for maintaining the nutritional value and extending the shelf life of blueberries. However, anthocyanin loss and off-flavor compound generation during fermentation impair blueberry wine color and quality. Hydroxycinnamate decarboxylase from yeast can catalyze the conversion of hydroxycinnamic acids to vinylphenols, which later may condense with anthocyanins to form more stable vinylphenolic pyranoanthocyanins.

Anti-Obesity Effects of Dietary Fibers Extracted from Flaxseed Cake in Diet-Induced Obese Mice.

Although many efforts have been made to characterize the functional properties of flaxseed, knowledge concerning the properties of insoluble and soluble dietary fibers in flaxseed is still limited. Here, insoluble and soluble dietary fibers were extracted from flaxseed cake-a valuable resource that has not been fully exploited. Subsequently, their monosaccharide compositions, structural properties, and anti-obesity effects in male mice were characterized.

Honokiol attenuates mitochondrial fission and cell apoptosis by activating Sirt3 in intracerebral hemorrhage.

Background: Sirtuin-3 (Sirt3) has been documented to protect against mitochondrial dysfunction and apoptosis. Honokiol (HKL) is a Sirt3 pharmacological activator with reported neuroprotective effects in multiple neurological disorders. The present study aimed to explore the neuroprotective effects of HKL and the role of Sirt3 following intracerebral hemorrhage (ICH).

Orexin-A exerts neuroprotective effect in experimental intracerebral hemorrhage by suppressing autophagy OXR1-mediated ERK/mTOR signaling pathway.

Background: Orexin-A (OXA) is a polypeptide produced in the hypothalamus, which binds to specific receptors and exerts multiple physiological effects. Autophagy plays a vital role in early brain injury (EBI) after intracerebral hemorrhage (ICH). However, the relationship between OXA and autophagy after ICH has not been confirmed.

Associations between body mass index and pubertal development based on the outcomes of girls with early breast development.

Objective: To investigate the associations between physical and pubertal development based on the breast development outcomes in girls.

Methods: This was a retrospective study. A total of 452 girls aged 6~8 years were included.

The Mycotoxin Beauvericin Exhibits Immunostimulatory Effects on Dendritic Cells Activating the TLR4 Signaling Pathway.

Beauvericin (BEA), a mycotoxin of the enniatin family produced by various toxigenic fungi, has been attributed multiple biological activities such as anti-cancer, anti-inflammatory, and anti-microbial functions. However, effects of BEA on dendritic cells remain unknown so far. Here, we identified effects of BEA on murine granulocyte-macrophage colony-stimulating factor (GM-CSF)-cultured bone marrow derived dendritic cells (BMDCs) and the underlying molecular mechanisms.

Rapid Body-Wide Transcriptomic Turnover During Rhesus Macaque Perinatal Development.

An hourglass cup-shape pattern of regulation at the molecular level was detected during the development of the primate brain. Specifically, a peak of temporally differentially expressed genes around the time of birth has been observed in the human brain. However, to what extend this peak of regulation exists among species has not been investigated in great detail.

Inhibition of PTEN Ameliorates Secondary Hippocampal Injury and Cognitive Deficits after Intracerebral Hemorrhage: Involvement of AKT/FoxO3a/ATG-Mediated Autophagy.

Spontaneous intracerebral hemorrhage (ICH) commonly causes secondary hippocampal damage and delayed cognitive impairments, but the mechanisms remain elusive. Here, we sought to identify the molecular mechanisms underlying these hemorrhagic outcomes in a rat autologous blood model of ICH. First, a significant increase in phosphatase and tensin homolog (PTEN) expression was observed in nonhemorrhagic ipsilateral hippocampus.

The Association of Polymorphisms in Base Excision Repair Genes with Ovarian Cancer Susceptibility in Chinese Women: A Two-Center Case-Control Study.

Base excision repair (BER) acts upon the most important mechanism of the DNA repair system, protecting DNA stability and integrity from the mutagenic and cytotoxic effects. Multiple researches have indicated that single-nucleotide polymorphisms (SNPs) in the BER-related gene may be associated with the susceptibility of ovarian cancer. However, the results are controversial.

Insights into the metabolic characteristics of aminopropanediol analogues of SYLs as S1P modulators: from structure to metabolism.

SYL927 and SYL930, two aminopropanediol analogues, are novel Sphingosine-1-phosphate receptor 1 (S1P) modulators with higher selectivity and pharmacological activity compared with FTY720. Although the immunosuppressive activity of SYLs has been well demonstrated, information regarding the metabolic fates of the two chemicals is limited except for the CYP-catalyzed hydroxylation of SYL930. In this study, the biotransformation schemes of the two promising chemicals were investigated and compared using liver microsomes, S9 fractions and recombinant enzymes, and relevant molecular mechanism was primarily demonstrated by ligand-enzyme docking analysis (CDOCKER).

Effect of nanoliposomal entrapment on antioxidative hydrolysates from goose blood protein.

In this study, the encapsulation of goose blood hydrolysate (GBH) was performed within nanoliposomes. We investigated the physicochemical properties, stability, antioxidant indices, the morphology of nanoparticles, the digestion stability in simulated gastrointestinal fluid, differential scanning calorimetry (DSC) analysis, and Fourier transform infrared (FTIR) spectroscopy. GBH was successfully encapsulated into nanoliposomes using reverse-phase evaporation method.

Association of polymorphisms in MALAT1 with the risk of endometrial cancer in Southern Chinese women.

Background: Endometrial cancer is the most common gynecologic malignancy worldwide. Polymorphisms in MALAT1 have been demonstrated to play critical roles in cancer. However, the roles of MALAT1 polymorphisms in the etiology of endometrial cancer have not been well documented.

Association of polymorphisms in MALAT1 with the risk of endometriosis in Southern Chinese women.

Endometriosis is a common estrogen-dependent inflammatory disease characterized by the presence of endometrial-like tissue outside the uterine cavity, which causes infertility and pelvic pain. Polymorphisms in MALAT1 have been demonstrated to play crucial roles in many diseases. However, the roles of MALAT1 polymorphisms in the etiology of endometriosis have not been well documented.

A Study of the Mechanism of Binding between Neratinib and MAD2L1 Based on Molecular Simulation and Multi-spectroscopy Methods.

Background: Nilatinib is an irreversible tyrosine kinase inhibitor, which is used in the treatment of some kinds of cancer. To study the interaction between Neratinib and MAD2L1, a potential tumor target, is of guiding significance for enriching the medicinal value of Neratinib.

Method: The binding mechanism between Mitotic arrest deficient 2-like protein 1 (MAD2L1) and Neratinib under simulative physiological conditions was investigated by molecule simulation and multi-spectroscopy approaches.

Glioma stages prediction based on machine learning algorithm combined with protein-protein interaction networks.

Background: Glioma is the most lethal nervous system cancer. Recent studies have made great efforts to study the occurrence and development of glioma, but the molecular mechanisms are still unclear. This study was designed to reveal the molecular mechanisms of glioma based on protein-protein interaction network combined with machine learning methods.