Tracking the infection dynamics of in based on GFP labelling.

Introduction: root rot, caused by , has caused severe damage to the industry. Due to the unclear pathogenic mechanisms of on , the effective implementation of control measures has been greatly restricted.

Methods: An efficient protoplast preparation and genetic transformation system was established for FO-1, enabling real-time tracking of fungal colonization in .

Targeted therapy for recurrent and refractory Papillary craniopharyngioma: A Systematic Narrative Review.

Objective: To evaluate the efficacy and safety of targeted molecular therapy for recurrent and refractory papillary craniopharyngioma (PCP) and explore its potential as a neoadjuvant treatment.

Methods: This systematic and narrative review was performed following PRISMA guidelines to evaluate BRAF and MEK inhibitors in treating PCP. PubMed, EMBASE, and Web of Science were searched up to July 2025.

Degradation Mechanisms and Intelligent Recognition of PTFE Triboelectric Performance under Hygrothermal Cycling Environment.

In this study, we systematically investigate the triboelectric performance degradation of polytetrafluoroethylene (PTFE)-based triboelectric nanogenerators (TENGs) under high-low temperature cycling and humidity variations, a critical yet underexplored challenge in extreme environmental applications. Under accelerated aging conditions spanning 0-300 thermal cycles and 0-90% RH, PTFE films demonstrated progressively worsening electrical output, their short-circuit current declining steadily from 7.2 to 4.

LncRNA SNHG15 targets miR-200a-3p affects the proliferation, apoptosis, migration, and invasion of cervical cancer cells.

Background: Cervical cancer is a major challenge facing women's health worldwide. In China, its incidence and mortality rate rank first among female reproductive tract malignancies. SNHG15 has been found and proven to have important functions in many cancers.

Use of integrated spatial transcriptomics and histopathological analysis in adamantinomatous craniopharyngiomas to identify stromal cells as a new cellular source of leukemia inhibitory factor.

Objective: Adamantinomatous craniopharyngioma (ACP) is an epithelial tumor that occurs in the sellar region of the human brain. Despite resection, relapse is frequent with poor prognosis. To facilitate the development of new therapy for ACP, the authors examined the spatial distribution, cell of origin, and potential biological functions of leukemia inhibitory factor (LIF), an important stem cell self-renewal regulator, in a series of ACP tumors.

Comparative analysis of clinical features, methylation and immune microenvironment in pediatric and adult papillary craniopharyngiomas: results from a multicenter study.

Papillary craniopharyngioma (PCP) was previously believed to occur only in adults. Sporadic pediatric PCP (PPCP), confirmed by detection of the BRAF V600E mutation, has been reported since 2018. The differences between PPCP and adult PCP (APCP) remain unclear.

Targeted therapy of papillary craniopharyngioma.

This review provide a comprehensive overview of the molecular biology and therapeutic advances regarding papillary craniopharyngiomas (PCP), with a particular focus on the pivotal role of the BRAF V600E mutation in its pathogenesis. Histopathologically, PCPs are characterized by stratified squamous epithelium and are frequently associated with the BRAF V600E mutation. This mutation activates the MAPK/ERK signaling pathway, which drives tumor development and progression.

A customized image editing framework for diverse prohibited and restricted products in illegal online transactions.

The circulation of prohibited and restricted goods in online transactions seriously violates consumer rights and threatens public safety. However, the lack of a dataset for prohibited and restricted goods makes it difficult to regulate such illegal online transactions. Therefore, a multimodal dataset for prohibited and restricted goods is proposed, including 38,513 images and 77,026 texts.

Nonsteroidal anti-inflammatory drug use and acute kidney injury in nephrectomies: A retrospective propensity score-matched cohort study.

Introduction: Nonsteroidal anti-inflammatory drugs (NSAID) are analgesic and spare opioids, but it remains unclear whether perioperative NSAID use worsens renal function after nephrectomy. We therefore tested the hypothesis that perioperative use of NSAID is associated with acute kidney injury (AKI) after nephrectomy surgery.

Methods: This retrospective cohort study included patients ≥18 years old who had partial or radical nephrectomies.

[Research progress in biomechanics of different fixation methods for medial opening-wedge high tibial osteotomy].

Objective: To summarize the biomechanical research progress on different fixation methods in medial opening-wedge high tibial osteotomy (MOWHTO) and provide references for selecting appropriate fixation methods in clinical applications of MOWHTO for treating knee osteoarthritis (KOA).

Methods: Recent domestic and international literature on the biomechanical studies of MOWHTO fixation methods was reviewed to analyze the characteristics and biomechanical performance of various fixation techniques.

Results: The medial-specific osteotomy plate system has become the mainstream due to its high stiffness and stability, but issues such as soft tissue irritation and stress shielding remain.

Exploring the Potential Value of Modulation of Cell Death in Immunotherapy of Gynecological Tumors.

Cell death plays a pivotal role in a multitude of biological processes, including embryonic development, organ maintenance, aging, immune response, and autoimmunity. These processes are underpinned by distinct molecular mechanisms and have significant implications for biological systems. Currently, research on regulatory cell death (RCD) is primarily focused on apoptosis, necroptosis, pyroptosis, ferroptosis, cuproptosis, and autophagy.

[Research progress in three-dimensional-printed bone scaffolds combined with vascularized tissue flaps for segmental bone defect reconstruction].

Objective: To review and summarize the research progress on repairing segmental bone defects using three-dimensional (3D)-printed bone scaffolds combined with vascularized tissue flaps in recent years.

Methods: Relevant literature was reviewed to summarize the application of 3D printing technology in artificial bone scaffolds made from different biomaterials, as well as methods for repairing segmental bone defects by combining these scaffolds with various vascularized tissue flaps.

Results: The combination of 3D-printed artificial bone scaffolds with different vascularized tissue flaps has provided new strategies for repairing segmental bone defects.

Comparative Efficacy of Swim-Up, Density-Gradient Centrifugation, and Microfluidic Sorting in Sperm Preparation, and the Impact on Motility, Morphology, and DNA Integrity.

Background:  Reactive oxygen species (ROS) are considered a major factor contributing to sperm DNA damage during sperm preparation for assisted reproductive technologies (ART). This study aimed to investigate whether microfluidic sorting can select sperm with a low DNA fragmentation index (DFI) and to explore the underlying mechanisms. We compared the effects of three sperm preparation methods-swim-up, density-gradient centrifugation, and microfluidic sorting-on sperm quality and DNA integrity.

Single-Cell Profiling of Tubular Epithelial Cells in Adaptive State in the Urine Sediment of Patients With Early and Advanced Diabetic Kidney Disease.

Introduction: Tubular epithelial cells (TECs) under adaptive state (aTECs) have been frequently reported in the injured kidney closely associated with disease progression. However, whether aTECs is present in the urine of patients with diabetic kidney disease (DKD) and its clinical implication have not been assessed.

Methods: Urine samples from patients with early and advanced DKD were collected and subjected to single-cell RNA sequencing (scRNA-seq).

Integrating bulk and single-cell RNA sequencing reveals SH3D21 promotes hepatocellular carcinoma progression by activating the PI3K/AKT/mTOR pathway.

As a novel genetic biomarker, the potential role of SH3D21 in hepatocellular carcinoma remains unclear. Here, we decipher the expression and function of SH3D21 in human hepatocellular carcinoma. The expression level and clinical significance of SH3D21 in hepatocellular carcinoma patients, the relationship between SH3D21 and the features of tumor microenvironment (TME) and role of SH3D21 in promoting hepatocellular carcinoma progression were analyzed based on the bulk samples obtained from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) databases.

[Mechanism of Daotan Xixin Decoction in treating APP/PS1 mice based on high-throughput sequencing technology and bioinformatics analysis].

This study aims to investigate the therapeutic effect and mechanism of Daotan Xixin Decoction on APP/PS1 mice. Twelve APP/PS1 male mice were randomized into four groups: APP/PS1 and low-, medium-, and high-dose Daotan Xixin Decoction. Three C57BL/6 wild-type mice were used as the control group.

Single-cell sequencing reveals that AK5 inhibits apoptosis in AD oligodendrocytes by regulating the AMPK signaling pathway.

Background: Neuroinflammation and abnormal energy metabolism have been shown to significantly contribute to the progression of Alzheimer's disease (AD). Adenylate kinase 5 (AK5), an enzyme predominantly expressed in the brain regulates ATP metabolism, has an unclear role in energy metabolism and neuroinflammation in AD.

Methods: The AD datasets were derived from the GEO public database to analyze the expression levels of AK5 in AD and normal samples and to assess the relationship between AK5 expression and the clinical characteristics of AD patients.

Evaluating the feasibility of AI-predicted bpMRI image features for predicting prostate cancer aggressiveness: a multi-center study.

Objective: To evaluate the feasibility of utilizing artificial intelligence (AI)-predicted biparametric MRI (bpMRI) image features for predicting the aggressiveness of prostate cancer (PCa).

Materials And Methods: A total of 878 PCa patients from 4 hospitals were retrospectively collected, all of whom had pathological results after radical prostatectomy (RP). A pre-trained AI algorithm was used to select suspected PCa lesions and extract lesion features for model development.

Peritoneal adipose stem cell-derived extracellular vesicles mediate the regulation of ovarian cancer cell proliferation and migration through EGFR-NF-κB signaling.

Peritoneal dissemination frequently develops in patients with ovarian cancer (OC) and is associated with recurrence and metastasis. However, the cellular components and mechanisms supporting OC peritoneal metastasis are poorly understood. To elucidate these, we utilized RNA sequencing to investigate the cellular composition and function.

Exosomes and non-coding RNAs: bridging the gap in Alzheimer's pathogenesis and therapeutics.

Alzheimer's disease (AD) is a neurodegenerative disease that primarily affects the elderly population and is the leading cause of dementia. Meanwhile, the vascular hypothesis suggests that vascular damage occurs in the early stages of the disease, leading to neurodegeneration and hindered waste clearance, which in turn triggers a series of events including the accumulation of amyloid plaques and Tau protein tangles. Non-coding RNAs (ncRNAs), including long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs), have been found to be involved in the regulation of AD.

Paromomycin targets HDAC1-mediated SUMOylation and IGF1R translocation in glioblastoma.

Objective: This study investigates the effects of Paromomycin on SUMOylation-related pathways in glioblastoma (GBM), specifically targeting HDAC1 inhibition.

Methods: Using TCGA and GTEx datasets, we identified SUMOylation-related genes associated with GBM prognosis. Molecular docking analysis suggested Paromomycin as a potential HDAC1 inhibitor.

Genome Mining Reveals Biosynthesis of the Antifungal Lipopeptaibols, Texenomycins, through a Hybrid PKS-NRPS System, in the Fungus .

Texenomycins are a family of linear lipopeptaibols with a long polyketide side chain at the N-terminus and 21 amino acid residues at the -terminus, presenting demonstrated potential as antibiotics against plant fungal pathogens. In this study, texenomycins were identified and isolated from the fungus strain TTI-0396 and showed effective antifungal properties against two plant pathogens and . Through analysis of the whole-genome data of strain TTI-0396, we discovered a hybrid PKS-NRPS system with the polyketide synthase (PKS: TexQ), thioesterase (TexO), acyl-CoA ligase (TexI), and three nonribosomal peptide synthetases (NRPSs: TexG, TexJ, TexV) in the gene cluster that were proposed to be responsible for the biosynthesis of texenomycins and another related lipopeptaibol, lipohexin.

Anti-tembusu virus of capsid-targeted viral inactivation delivered by lentiviral vector in vivo.

Tembusu virus (TMUV) is a member of genus flavivirus, which mainly causes decrease in production in egg ducks and neurological symptom in meatducks, causing serious economic losses to the poultry industry. Recently, the commercialized TMUV vaccines are mainly the WF100 live vaccine and the attenuated live vaccine (FX2010-180P), so it is particularly important to find new methods to combat TMUV. The capsid-targeted viral inactivation (CTVI) strategy is based on a viral core protein and an exogenous factor that can destroy viral DNA or RNA.