Decoupling of bacterial production and respiration in the surface water of the North Pacific Subtropical Gyre.

Unlabelled: Heterotrophic bacterial production and respiration, two important contributors to carbon cycling, play an important role in global biogeochemical cycles. However, recent research suggests that these two processes may be decoupled, and the underlying changes in community structure and their interactions remain unclear. In this study, two research expeditions to the North Pacific Subtropical Gyre (NPSG) during the summer and winter of 2020-2021 revealed seasonal shifts in bacterial metabolism and community structure in response to environmental factors.

Inhibition of hsa_circ_0003314 contributes to trophoblast cell migration and invasion and inhibits pyroptosis in preeclampsia.

Inflammation is a key contributor to the development of preeclampsia. Recent studies suggest that circular RNAs (circRNAs) may serve as potential therapeutic targets for this disease, though their specific functions remain incompletely understood. In this study, we investigated the role of hsa_circ_0003314 in preeclampsia pathogenesis.

Comparison of extracorporeal shock wave therapy and manual therapy on active trigger points of the sternocleidomastoid muscle in cervicogenic headache: A randomized controlled trial.

Objectives: This study aims to investigate the effect of extracorporeal shock wave therapy (ESWT) and manual therapy (MT) on active trigger points of the sternocleidomastoid muscle (SCM) in patients with cervicogenic headache (CEH).

Patients And Methods: A total of 42 patients were included (27 females, 15 males; mean age: 33.2±7.

Dynamics of Phytoplankton Communities and Their Characteristics of Realized Niches in a Drinking Reservoir.

Realized niches are crucial in defining their optimal conditions and serve as valuable tools for predicting the phytoplankton dynamics in relation to eutrophication, climate change, and harmful phytoplankton blooms. However, previous studies have largely focused on marine ecosystems, leaving freshwater systems less studied. In this study, we elucidate the patterns of phytoplankton community succession based on their niche characteristics in the Shanmei (SM) Reservoir, a drinking water source in Quanzhou, Fujian Province.

Bayesian nonparametric latent class analysis with different item types.

Latent class analysis (LCA) requires deciding on the number of classes. This is traditionally addressed by fitting several models with an increasing number of classes and determining the optimal one using model selection criteria. However, different criteria can suggest different models, making it difficult to reach a consensus on the best criterion.

Enhancing short-term algal bloom forecasting through an anti-mimicking hybrid deep learning method.

Accurately predicting algal blooms remains a critical challenge due to their dynamic and non-stationary nature, compounded by high-frequency fluctuations and noise in monitoring data. Additionally, a common issue in time-series forecasting is data replication, where models tend to replicate historical patterns rather than capturing true future variations, leading to inaccurate forecasts during abrupt changes. To address these challenges, we developed a hybrid deep learning model (TAB) that integrates a Temporal Convolutional Network (TCN), an attention mechanism, and Bidirectional Long Short-Term Memory (BiLSTM) network.

Effect and Mechanism of Apple Polyphenols in Regulating Intestinal Flora and Inhibiting the TLR4/NF-κB/TGF-β Signaling Pathway to Alleviate Alcoholic Liver Fibrosis.

Apple polyphenols (APs) have gained attention for their various bioactivities, while no studies on anti-liver fibrosis activity are reported. This study evaluated the protective effect of APs on liver fibrosis using LPS-treated activated HSC-T6 cells and alcohol-treated liver fibrosis (ALF) mice. The results indicated that APs inhibited HSC-T6 cells activation in vitro and reduced the level of serum hyaluronic acid (HA) (p < 0.

A pedigree analysis of Rotor hyperbilirubinemia combined with hepatitis B virus infection in a and gene mutations patient.

Background: Rotor syndrome (RS, OMIM#237450) is an extremely rare autosomal digenic recessive disorder characterized by mild non-hemolytic hereditary conjugated hyperbilirubinemia, caused by biallelic variation of and genes that resulted in OATP1B1/B3 dysfunction in the sinusoidal membrane leading to impaired bilirubin reuptake ability of hepatocytes.

Methods: One RS pedigree was recruited and clinical features were documented. Whole genome second-generation sequencing was used to screen candidate genes and mutations, Sanger sequencing confirmed predicted mutations.

Research waste among randomized controlled trials in ovarian cancer: A cross-sectional study.

Objective: To examine the association between trial characteristics and research waste in randomized controlled trials (RCTs) on ovarian cancer over the past two decades.

Methods: ClinicalTrials.gov was searched for RCTs registered between 2000 and 2020 using the keyword ovarian cancer.

PTK6: An emerging biomarker for prognosis and immunotherapeutic response in clear cell renal carcinoma (KIRC).

Kidney renal clear cell carcinoma (KIRC), one of the most prevalent form of kidney carcinoma, is highly aggressive cancer known for significant immune infiltration and high mortality rates. The absence of sensitivity to traditional therapy has spurred the search for new treatments. Protein Tyrosine Kinase 6 (PTK6) is implicated in promoting cancer growth, spread, and metastasis.

Biological Control of Avocado Branch Blight Caused by Using .

In recent years, avocado branch blight has gradually become one of the major diseases causing mortality of avocado trees, which seriously affects the economic development of avocado planting regions. In order to investigate the cause of the disease, the pathogens were isolated from the interroot of avocado trees with the onset of the disease and identified as . At the same time, three strains, YK194, YK201, and YK268, with better antagonistic effects and high stability against , were isolated from the rhizospheric soil of healthy avocado plants.

Amino Acid Derivatives of Ginsenoside AD-2 Induce HepG2 Cell Apoptosis by Affecting the Cytoskeleton.

(20(R)-dammarane-3β, 12β, 20, 25-tetrol, 25-OH-PPD) was structurally modified to introduce additional amino groups, which can better exert its anti-tumor effects in MCF-7, A549, LoVo, HCT-116, HT -29, and U-87 cell lines. We investigated the cellular activity of 15 different amino acid derivatives on HepG2 cells and the possible mechanism of action of the superior derivative . An MTT assay was used to detect the cytotoxicity of the derivatives.

Erythrocyte ENT1-AMPD3 Axis is an Essential Purinergic Hypoxia Sensor and Energy Regulator Combating CKD in a Mouse Model.

Significance Statement: Hypoxia drives kidney damage and progression of CKD. Although erythrocytes respond rapidly to hypoxia, their role and the specific molecules sensing and responding to hypoxia in CKD remain unclear. In this study, we demonstrated in a mouse model that erythrocyte ENT1-AMPD3 is a master energy regulator of the intracellular purinergic hypoxic compensatory response that promotes rapid energy supply from extracellular adenosine, eAMPK-dependent metabolic reprogramming, and O 2 delivery, which combat renal hypoxia and progression of CKD.

Tissue transglutaminase: a multifunctional and multisite regulator in health and disease.

Tissue transglutaminase (TG2) is a widely distributed multifunctional protein involved in a broad range of cellular and metabolic functions carried out in a variety of cellular compartments. In addition to transamidation, TG2 also functions as a Gα signaling protein, a protein disulfide isomerase (PDI), a protein kinase, and a scaffolding protein. In the nucleus, TG2 modifies histones and transcription factors.

Application of the 6S management strategies.

Aim: To explore the value of the 6S management strategies in COVID-19 wards.

Design: Nurses from the first Fujian medical team in aiding Hubei Province at Wuhan Jinyintan Hospital (2 February, 2020-24 March, 2020) were recruited and instructed to adopt the 6S management strategies for nursing management.

Methods: The paired-sample t-test was performed to compare the nursing quality, core capabilities of nurses, nursing preparation time, and nursing error rates before and after implementation.

Inulin ameliorates metabolic syndrome in high-fat diet-fed mice by regulating gut microbiota and bile acid excretion.

Inulin is a natural plant extract that improves metabolic syndrome by modulating the gut microbiota. Changes in the gut microbiota may affect intestinal bile acids. We suggest that inulin may improve metabolism by inducing bile acid excretion by gut microbes.

Stiffness changes in internal rotation muscles of the shoulder and its influence on hemiplegic shoulder pain.

Background: Hemiplegic shoulder pain (HSP) is a common complication in patients with stroke. The pathogenesis of HSP is complex, and muscle hypertonia, especially the hypertonia of internal rotation muscles of the shoulder, may be one of the important causes of shoulder pain. However, the relationship between muscle stiffness and HSP has not been well studied.

A human pan-cancer system analysis of heat shock protein family A member 5.

Heat shock protein family A member 5 (HSPA5) plays a pivotal role in the endoplasmic reticulum (ER) stress response and unfolded protein response (UPR), both of which are crucial for protein folding, assembly, and quality control within cells. In response to ER stress, HSPA5 becomes overexpressed to preserve cellular homeostasis. A previous study revealed a robust association between HSPA5 expression and various cancers.

Transcriptome profiling of genes regulated by phosphate-solubilizing bacteria P68 in potato ( L.).

The insoluble phosphorus in the soil is extremely difficult to be absorbed and used directly through the potato root system. Although many studies have reported that phosphorus-solubilizing bacteria (PSB) can promote plant growth and uptake of phosphorus, the molecular mechanism of phosphorus uptake and growth by PSB has not been investigated yet. In the present study, PSB were isolated from rhizosphere soil in soybean.

Identification of new immune subtypes of renal injury associated with anti-neutrophil cytoplasmic antibody-associated vasculitis based on integrated bioinformatics analysis.

Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a systemic autoimmune disease that may lead to end-stage renal disease. However, few specifific biomarkers are available for AAV-related renal injury. The aim of this study was to identify important biomarkers and explore new immune subtypes of AAV-related renal injury.

Pregnancy-induced changes to the gut microbiota drive macrophage pyroptosis and exacerbate septic inflammation.

The physiological and immune changes that occur during pregnancy are associated with worsened disease outcomes during infection and sepsis. How these perturbations exacerbate inflammation has not been explored. Here, using antibiotic treatment and fecal microbial transfers, we showed that sepsis susceptibility is driven by pregnancy-induced changes to gut microbiome in mice and humans.

Ginsenoside AD-2 Ameliorating Lipopolysaccharide-Induced Activation in HSC-T6 Cells and Carbon Tetrachloride-Induced Hepatic Fibrosis in Mice Regulation of VD-VDR Axis.

Ginsenoside 25-hydroxy protopanaxadiol (AD-2) isolated from ginseng was proved to have anti-hepatic fibrosis (HF) effect in our previous study. But the mechanism is unknown. The present study investigated the anti-HF effects and mechanisms of AD-2 on the lipopolysaccharide (LPS)-induced activation in HSC-T6 cells and carbon tetrachloride (CCl)-induced hepatic fibrosis (HF) in mice.

Update on new trend and progress of the mechanism of polysaccharides in the intervention of Alzheimer's disease, based on the new understanding of relevant theories: A review.

Alzheimer's disease (AD), a neurodegenerative disease with insidious onset and progressive progression, is the main type of dementia. Currently, there is no specific cure for the disease. At the same time, a series of drug developments based on the classic theory, the Aβ cascade hypothesis, have not completed phase III clinical trials, challenging the hypothesis.