Piezoresistive MOF for Intraocular Pressure Monitoring.

Continuous monitoring of intraocular pressure (IOP) is critical for the diagnosis and management of glaucoma. Flexible piezoresistive wearable devices, featuring high sensitivity and simple signal processing, enable such continuous monitoring. Recently, conductive metal-organic frameworks (MOFs) have shown promising potential in electronic devices due to their coordination self-assembly, which allows integration with various complex substrates for wearable sensor applications.

Mass spectrometry-based characterization of histone post-translational modification.

Histone post-translational modifications (PTMs) play critical roles in regulating chromatin dynamics and gene expression. Increasing evidence demonstrates that the dysregulation of histone PTMs is closely associated with the pathogenesis of various diseases. Traditional methods for detecting histone PTMs, such as western blot (WB) and chromatin immunoprecipitation sequencing (ChIP-seq), are often limited by their dependence on specific antibodies and relatively low analytical throughput.

Dl-3-n-butylphthalide attenuates acute myocardial infarction by inhibiting the binding of Nrf2/Keap1.

Acute myocardial infarction (AMI) is a prevalent cardiovascular condition often encountered in clinics. It is characterized by an imbalance in the myocardial oxygen supply and demand and is thought to be associated to cell apoptosis and oxidative stress. Dl-3-n-butylphthalide (NBP), a natural product extracted from celery for the clinical treatment of ischemic stroke, may mitigate the effects of myocardial infarction.

High-Sensitivity All-Fiber Sensor Smart Gloves for Hand Perception.

Flexible piezoresistive sensors (FPSs) with high sensitivity and conformability are crucial for achieving fine operations in electronic gloves. In this work, a chemical grafting method is used to ensure strong interfacial bonding between the conductive phase and the flexible polyimide (PI) matrix with a high glass transition temperature (). This design helps to tackle the stress relaxation and interfacial debonding problems commonly faced by FPSs.

Uncovering differences in cadmium accumulation capacity of different cultivars at the level of root cell types.

Water spinach () can accumulate cadmium (Cd) even in mildly contaminated soils, but the roles of its root tip cell types in Cd fixation and transport remain unclear. Single-cell RNA sequencing revealed nine cell types in root tips in both the QLQ cultivar (low Cd accumulation) and the T308 cultivar (high Cd accumulation). High expression of and in the QLQ epidermis was associated with enhanced lignin deposition, which may facilitate fixation of Cd and reduce its translocation to the shoot.

Bioinspired Interlocked Nanostructured Piezoresistive Composite for Monitoring of Renal Pelvic Pressure.

Inspired by the structure of and based on guidance of molecular dynamics simulations, a hierarchical nanospike structure on micrometer-sized coaxial fibers has been designed at the molecular scale. A piezoresistive composite membrane of in situ-grown PDA-PPy on a TPU@PES coaxial fiber has been prepared, exhibiting good anticreep performance, high sensitivity, and fast response. The matrix material is designed as coaxial fibers, which consist of an inner PES core that provides anticreep mechanical support and an outer thermoplastic polyurethane shell that offers a large specific surface area and rich graft reaction sites.

Quantitation of global histone post-translational modifications reveal anti-inflammatory epigenetic mechanisms of liquiritigenin based on the optimized super-SILAC strategy.

Liquiritigenin (LIQ) is a dihydroflavonone monomer compound with a planar ring structure that exhibits potent anti-inflammatory activity. The post-translational modifications (PTMs) of histones are closely associated with inflammatory diseases. To explore the relationships between the anti-inflammatory effects and epigenetic regulatory mechanisms of LIQ, we optimized the super stable isotope labeling by amino acids in cell culture (super-SILAC) method combined with a compound stimulation strategy.

Typhimurium persistently infects host via its effector SseJ-induced PHB2-mediated mitophagy.

Despite decades of research on effective methods to resist serovar Typhimurium (. Typhimurium) pathogenicity, the mechanisms of . Typhimurium-host interactions have not been fully determined.

Newly identified c-di-GMP pathway putative EAL domain gene STM0343 regulates stress resistance and virulence in Salmonella enterica serovar Typhimurium.

S. Typhimurium is a significant zoonotic pathogen, and its survival and transmission rely on stress resistance and virulence factors. Therefore, identifying key regulatory elements is crucial for preventing and controlling S.

Flexural Behavior of BFRP Bar-Recycled Tire Steel Fiber-Reinforced Concrete Beams.

This research advocates for the use of basalt fiber-reinforced polymer (BFRP) bars and recycled tire steel fibers to reinforce concrete beams. Six concrete beams were constructed using different volume contents of recycled tire steel fibers (0, 0.5%, 1.

Tantalum-stabilized ruthenium oxide electrocatalysts for industrial water electrolysis.

The iridium oxide (IrO) catalyst for the oxygen evolution reaction used industrially (in proton exchange membrane water electrolyzers) is scarce and costly. Although ruthenium oxide (RuO) is a promising alternative, its poor stability has hindered practical application. We used well-defined extended surface models to identify that RuO undergoes structure-dependent corrosion that causes Ru dissolution.

Smart Driving Hardware Augmentation by Flexible Piezoresistive Sensor Matrices with Grafted-on Anticreep Composites.

Signal drift and hysteresis of flexible piezoresistive sensors pose significant challenges against the widespread applications in emerging fields such as electronic skin, wearable equipment for metaverse and human-AI (artificial intelligence) interfaces. To address the creep and relaxation issues associated with pressure-sensitive materials, a highly stable piezoresistive composite is proposed, using polyamide-imide (PAI) fibers as the matrix and in situ grafted-polymerized polyaniline (PANI) as the semi-conducting layer. The PAI with large rigid fluorenylidene groups exhibits a high glass transition temperature of 372 °C (PAI 5-5), which results in an extremely long relaxation time at room temperature and consequently offers outstanding anti-creep/relaxation performances.

Multi-step HPLC fractionation enabled in-depth and unbiased characterization of histone PTMs.

Histone post-translational modifications (PTMs) are critical epigenetic regulatory factors. Histone PTMs are highly dynamic and complicated, encompassing over 30 structurally diverse modifications across nearly 180 amino acid residues, which generated extensive information regarding histone marks. In proteomics-based characterization of histone PTMs, chemical derivatization and antibody-based affinity enrichment were frequently utilized to improve the identification depth.

Establishing Virtual Bioequivalence and Clinically Relevant Specifications for Omeprazole Enteric-Coated Capsules by Incorporating Dissolution Data in PBPK Modeling.

Currently, Biopharmaceutics Classification System (BCS) classes I and III are the only biological exemptions of immediate-release solid oral dosage forms eligible for regulatory approval. However, through virtual bioequivalence (VBE) studies, BCS class II drugs may qualify for biological exemptions if reliable and validated modeling is used. Here, we sought to establish physiologically based pharmacokinetic (PBPK) models, in vitro-in vivo relationship (IVIVR), and VBE models for enteric-coated omeprazole capsules, to establish a clinically-relevant dissolution specification (CRDS) for screening BE and non-BE batches, and to ultimately develop evaluation criteria for generic omeprazole enteric-coated capsules.

Substrate and Functional Diversity of Protein Lysine Post-translational Modifications.

Lysine post-translational modifications (PTMs) are widespread and versatile protein PTMs that are involved in diverse biological processes by regulating the fundamental functions of histone and non-histone proteins. Dysregulation of lysine PTMs is implicated in many diseases, and targeting lysine PTM regulatory factors, including writers, erasers, and readers, has become an effective strategy for disease therapy. The continuing development of mass spectrometry (MS) technologies coupled with antibody-based affinity enrichment technologies greatly promotes the discovery and decoding of PTMs.

Chiral Osmium(II)/Salox Species Enabled Enantioselective γ-C(sp)-H Amidation: Integrated Experimental and Computational Validation For the Ligand Design and Reaction Development.

Herein, multiple types of chiral Os(II) complexes have been designed to address the appealing yet challenging asymmetric C(sp)-H functionalization, among which the Os(II)/Salox species is found to be the most efficient for precise stereocontrol in realizing the asymmetric C(sp)-H amidation. As exemplified by the enantioenriched pyrrolidinone synthesis, such tailored Os(II)/Salox catalyst efficiently enables an intramolecular site-/enantioselective C(sp)-H amidation in the γ-position of dioxazolone substrates, in which benzyl, propargyl and allyl groups bearing various substituted forms are well compatible, affording the corresponding chiral γ-lactam products with good er values (up to 99 : 1) and diverse functionality (>35 examples). The unique performance advantage of the developed chiral Os(II)/Salox system in terms of the catalytic energy profile and the chiral induction has been further clarified by integrated experimental and computational studies.

Enantioselective Tsuji-Trost α-Fluoroallylation of Amino Acid Esters with Gem-Difluorinated Cyclopropanes.

A novel enantioselective Tsuji-Trost-type cross coupling reaction between gem-difluorinated cyclopropanes and N-unprotected amino acid esters enabled by synergistic Pd/Ni/chiral aldehyde catalysis is presented herein. This transformation streamlined the diversity-oriented synthesis (DOS) of optically active α-quaternary α-amino acid esters bearing a linear 2-fluoroallylic motif, which served as an appealing platform for the construction of other valuable enantioenriched compounds. The key intermediates were confirmed by HRMS detection, while DFT calculations revealed that the excellent enantioselectivity was attributed to the stabilizing non-covalent interactions between the Pd(II)-π-fluoroallyl species and the Ni(II)-Schiff base complex.

Targeting the NLRP3 inflammasome in psoriasis.

The NLRP3 inflammasome, a complex consisting of the nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing protein 3, has emerged as a critical mediator of pathological inflammation and a significant therapeutic target for various inflammatory diseases. Psoriasis, a chronic inflammatory skin condition without a definitive cure, has shown promising results in animal models through the inhibition of the NLRP3 inflammasome. This review aims to explore the development of the NLRP3 inflammasome in psoriasis and the molecular mechanisms responsible for its inhibition by natural products and small molecules currently being developed for psoriasis treatment.

BolA promotes the generation of multicellular behavior in S. Typhimurium by regulating the c-di-GMP pathway genes yeaJ and yhjH.

The generation of multicellular behavior enhances the stress adaptability, antibiotic resistance, and pathogenic potential of Salmonella enterica serovar Typhimurium (S. Typhimurium), which is challenging for its prevention and control. Therefore, determination of the mechanism of multicellular behavior development is urgently required.

High Levels of Antibiotic Resistance in MDR-Strong Biofilm-Forming Typhimurium ST34 in Southern China.

enterica subsp. enterica serovar Typhimurium (. ) is an important zoonotic pathogen with important public health significance.

Ultra-Tough Waterborne Polyurethane-Based Graft-Copolymerized Piezoresistive Composite Designed for Rehabilitation Training Monitoring Pressure Sensors.

Effective training is crucial for patients who need rehabilitation for achieving optimal recovery and reducing complications. Herein, a wireless rehabilitation training monitoring band with a highly sensitive pressure sensor is proposed and designed. It utilizes polyaniline@waterborne polyurethane (PANI@WPU) as a piezoresistive composite material, which is prepared via the in situ grafting polymerization of PANI on the WPU surface.

BolA affects the biofilm formation ability, outer membrane permeability and virulence, thus is required for the adaptability of Salmonella enterica serotype Typhimurium to the harsh survival environment.

Salmonella enterica serotype Typhimurium, an important foodborne pathogen with high adaptability to the host's internal and external survival environment, seriously threatens public health. Therefore, to understand the mechanism underlying the high adaptability, this study investigated the transcription factor BolA by constructing BolA deletion strain 269△BolA, complemented strain 269BolAR and overexpression strain 269BolA+ based on WT269. BolA significantly inhibited motility; at 6 h, the BolA overexpression strain (269BolA+) showed 91.