Porous microscaffold enables spontaneous hematopoietic cell differentiation and continuous macrophage production from hPSCs for therapy.

Macrophages play critical roles in various physiological and pathological processes, yet their scalable production for therapeutic applications remains a challenge. Here, we present a porous microscaffold (PMS) that enables robust hematopoietic differentiation from human pluripotent stem cell (hPSC)-derived mesoderm. Within PMS, mesoderm cells self-organize into vascular structures reminiscent of the early aorta-gonad-mesonephros (AGM) niches, promoting efficient endothelial-to-hematopoietic transition with minimal cytokine supplementation.

Hydrological niche segregation and water-use strategies of coexisting plant species evidenced by water-stable isotopes in a hyper-arid endorheic basin.

Better understanding of the mechanisms of plant species' coexistence and close interactions between vegetation and groundwater in drylands is indispensable for predicting the potential consequences of environmental changes. These interrelationships, however, remain an understudied topic. In this study, 262 sample plots along water table depth (WTD) gradients (0 to 5 m, 5 to 10 m, 10 to 20 m, and > 20 m) were investigated in a hyper-arid endorheic basin (the Shule River Basin, SRB) in northwestern China.

Genome-wide analysis of the DnaJ gene family and their expression profiles under salt stress in potato.

Background: Saline stress is one of the primary environmental factors affecting global food security, significantly jeopardizing crop quality and yield. DnaJ proteins (DnaJs) are a type of protein that plays an important role in dealing with salt stress for plants. Nevertheless, the comprehensive exploration of the DnaJ gene family and its role in salt stress in potato (Solanum tuberosum L.

BPA disrupts crustacean reproduction: Tissue-specific accumulation, oxidative stress, and gene dysregulation in Macrobrachium nipponense.

Bisphenol A (BPA), a pervasive environmental endocrine disruptor, increasingly threatens aquatic ecosystems due to its reproductive toxicity. This study investigates how BPA disrupts ovarian development in Macrobrachium nipponense - by analyzing its tissue-specific bioaccumulation (ovaries, hepatopancreas, fertilized eggs), antioxidant responses, and developmental gene regulation. Experimental results demonstrated that after 10-day exposure to 10, 100, and 1000 μg/L BPA, significant BPA accumulation was observed in both ovaries and hepatopancreas, while fertilized eggs showed notable accumulation only at the highest concentration (1000 μg/L) (P < 0.

Prognostic analysis and development of a predictive model for pulmonary invasive mucinous adenocarcinoma.

Background: Pulmonary invasive mucinous adenocarcinoma (IMA), a rare subtype of lung adenocarcinoma, exhibits distinct clinicopathological features. However, its prognostic determinants remain poorly understood due to its low incidence and the limited availability of longitudinal survival data. Diagnostic challenges complicate the management of patients with IMA, as it often mimics inflammatory or infectious pulmonary lesions.

Lumbar disc herniation reabsorption: a review of clinical manifestations, mechanisms, and conservative treatments.

Lumbar disc herniation (LDH) is a common and frequently occurring condition primarily caused by lumbar intervertebral disc degeneration (LIVDD) and protrusion of the nucleus pulposus (NP), with low back pain and lower limb pain as the main clinical manifestations. It is characterized by a prolonged disease course and a high recurrence rate, with patients often experiencing long-term suffering, significantly impairing their quality of life and mental health. Studies have found that some LDH patients, without undergoing surgery or chemonucleolysis, experience spontaneous shrinkage and resorption of the intervertebral disc (IVD) tissue, along with relief of back and leg pain-a phenomenon referred to as LDH reabsorption.

[PI-RADS v2.1 score combined with PSA density for diagnosis of clinically significant prostate cancer in the PSA grey zone by MRI-TRUS cognitivefusion-guided transperineal targeted prostate biopsy].

Objective: To assess the value of the Prostate Imaging Reporting and Data System version 2.1 (PI-RADS v2.1) score combined with PSA density (PSAD) in the diagnosis of clinically significant prostate cancer (CSPCa) in the PSA grey zone by MRI-TRUS cognitive fusion-guided transperineal targeted prostate biopsy.

The relationship between air pollution and dementia in mood disorders: The moderating role of sleep patterns-- A prospective study from the UK Biobank.

Purpose: This study aims to quantify dementia risk in mood disorder patients, to examine the association between air pollution and dementia risk, and to assess whether sleep patterns incorporating five specific sleep behaviors modifies this relationship.

Methods: This prospective cohort study enrolled 459,635 individuals from the UK Biobank, 46,256 mood disorder patients at baseline; of those, 1494 developed dementia during follow-up. Air pollution exposure (PM, PM, PM, NO, and NO) was estimated using land-use regression models.

Letter to the editor: Sex difference in the association between dietary iron intake and bone mineral density in adolescents aged 12-19: A cross-sectional study.

We read with great interest the article by Li et al. (2025) [1] titled "Sex difference in the association between dietary iron intake and bone mineral density in adolescents aged 12-19: A cross-sectional study", recently published in the Journal of Trace Elements in Medicine and Biology. The authors present compelling evidence for a sex-specific relationship between dietary iron intake and bone mineral density (BMD), identifying a significant positive association in male adolescents but not in females.

Temporal variation in the association between heatwave and mortality from mental disorders: population-based evidence from a megacity of China.

Background: Prolonged hot weather poses a threat to mental health. However, little is known about whether patients with mental disorders have adapted to prolonged hot weather, namely a heatwave. We aimed to analyse temporal patterns in the effect of heatwaves on mental disorders and decompose the effect of heatwave components.

Influence of Recycled Tire Steel Fibers on the Mechanical Properties and Carbon Emissions of High-Performance Cement-Based Materials.

To address the issues of high carbon emissions from concrete and high energy consumption in the manufacturing of traditional steel fibers, this study investigates the feasibility of replacing industrial steel fibers (ISF) with recycled tire steel fibers (RSF) in high-performance cement-based materials. The study examines the effects of fiber type and dosage on the mechanical properties within the systems of ultra-high-performance concrete (UHPC) and slurry-infiltrated fiber concrete (SIFCON) and analyzes the carbon emission levels using the Life Cycle Assessment (LCA) method. Research results indicate that the compressive and tensile strengths of SIFCON are significantly higher than those of UHPC.

Cellular senescence and disulfide death-related genes as biological markers of breast cancer prognosis.

Objective: Breast cancer is a heterogeneous disease with diverse prognosis and treatment outcomes. The aim of this study was to reveal the genes related to cellular senescence and disulfide death in breast cancer and to explore their potential mechanisms and clinical applications in breast cancer.

Methods: In this study, we screened differential genes associated with cellular senescence and disulfide death based on publicly available data, constructed a protein-protein interaction network (PPI Network), and explored the functions of differential genes by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis.

Mechanisms and Factors Influencing Resorption of Herniated Part of Lumbar Disc Herniation: Comprehensive Review.

Abstract: This review systematically examines the mechanisms underlying the spontaneous resorption of lumbar disc herniation (LDH), focusing on vascularization, autophagy, apoptosis, macrophage activity, and the therapeutic potential of traditional Chinese medicine (TCM). We emphasize that resorption is influenced by factors such as herniation type, disease duration, and imaging biomarkers (eg, the bull's-eye sign). Additionally, this review summarizes recent progress in the study of resorption mechanisms following lumbar disc herniation, providing a reference for future clinical research.

Neonatal-Inspired Reprogramming of Microglial Pan-Programmed Cell Death Enhances Regeneration in Adult Spinal Cord Injury.

In adult mammals, programmed cell death (PCD) facilitates tissue remodeling and regeneration in spinal cord injury (SCI), but excessive activation impedes SCI repair. However, no comprehensive pan-PCD atlas exists that encompasses diverse cell death patterns to fully elucidate PCD in adult SCI and develop strategies for modulating the excessive PCD response. Here, we identified neonatal mice with balanced PCD post-SCI as an ideal model for adult SCI.

Unilateral biportal endoscopy for the treatment of symptomatic spinal epidural lipomatosis: a case report and literature review.

Background: Spinal epidural lipomatosis (SEL) is rare and often ignored or misdiagnosed. Traditional open surgery is considered the standard procedure for treating symptomatic SEL. However, open surgery is often associated with substantial trauma and a long recovery period.

Structural regulation of VScathodes for enhanced aqueous zinc-ion battery performance.

VShas garnered significant attentions in aqueous zinc ion batteries (AZIBs) due to its unique structural features and high theoretical capacity. Unfortunately, the volumetric changes and sluggish kinetics of VSduring the electrochemical process often lead to material degradation and structural collapse, thereby limiting the performance of AZIBs. To address these challenges, we propose a structural engineering strategy for VSto regulate its microstructure through a simple hydrothermal method.

Establishing and mapping heat-sensitive disease spectrum in eastern China: A comprehensive analysis of 1.4 million deaths involving 14 major disease categories.

Background: Although high temperatures can affect multiple systems and organs, the comprehensive assessment of heat-sensitive diseases remains unclear. We aimed to establish the heat-related sensitive disease spectrum and assess the relative importance of affected diseases from the health risk and burden perspectives.

Methods: A space-time-stratified case-crossover analysis was used to examine the short-term association between high temperatures and cause-specific deaths in Jiangsu Province, China during the warm season of 2016 to 2019.

Multisite Chronic Pain and the Risk of Breast Cancer and Its Subtypes: A Mendelian Randomization Study.

Background: Chronic pain (CP) is widespread and a major cause of disability. However, its genetic and environmental risk factors, as well as its relationship with breast cancer (BC), remain unclear. The study is the first to apply Mendelian randomization (MR) to explore the causal relationship between CP and BC.

Study on Early-Age Capillary Pressure and Plastic Shrinkage Properties of High-Volume Fly Ash Concrete.

There is a lack of research on the early plastic deformation and capillary pressure of high-volume fly ash concrete (HVFAC) under varying ambient temperatures. This study aims to investigate the effects of water-binder ratio, fly ash admixture, and ambient temperature on the air entry time , capillary pressure, and plastic shrinkage of HVFAC. Nine different fly ash concrete materials were designed and analyzed to determine the early plastic deformation and capillary pressure of HVFAC under different ambient temperatures.

Experimental study on the influence of sample preparation and testing methods on the shear mechanical properties of silt.

To investigate the influence of sample preparation conditions and testing methods on the shear properties of compacted silt, samples with different moisture contents were prepared by saturation and drying methods. The shear mechanical properties of the samples were tested by direct shear and triaxial compression tests. When the designed moisture content w of the sample is lower than the optimal moisture content w, the structural strength of the drying sample is higher than that of the water-added sample, and the brittle failure is more obvious in the drying method, and the shear strength and parameters are larger.

Identification of aging-related biomarkers for intervertebral disc degeneration in whole blood samples based on bioinformatics and machine learning.

Introduction: Aging is characterized by gradual structural and functional changes in the body over time, with intervertebral disc degeneration (IVDD) representing a key manifestation of spinal aging and a major contributor to low back pain (LBP).

Methods: This study utilized bioinformatics and machine learning approaches to identify aging-related biomarkers associated with IVDD in whole blood samples. By analyzing GEO datasets alongside aging-related databases such as GeneCards, HAGR, and AgeAnno, we identified 15 aging-related differentially expressed genes (AIDEGs).

[Orthopedic manipulation combined with percutaneous reduction and Kirschner wire internal fixation for Sanders typeⅡand Ⅲ calcaneal fractures].

Objective: To explore clinical effects of bone setting manipulation combined with pry reduction and Kirschner needle internal fixation in treating SandersⅡ-Ⅲ calcaneal fracture.

Methods: Clinical data of 52 patients with types Sanders Ⅱand Ⅲ calcaneal fracture (foot) treated with bone-setting manipulation combined with pry reduction and Kirscher needle internal fixation from July 2017 to July 2019 were retrospectively analyzed, including 43 males and 9 females, aged from 31 to 72 years old with an average of (50.83±10.

Recent applications of stimulus-responsive smart hydrogels for osteoarthritis therapy.

Osteoarthritis is one of the most common degenerative joint diseases, which seriously affects the life of middle-aged and elderly people. Traditional treatments such as surgical treatment and systemic medication, often do not achieve the expected or optimal results, which leads to severe trauma and a variety of side effects. Therefore, there is an urgent need to develop novel therapeutic options to overcome these problems.