Cross-reactivity mediated by SARS-CoV-2 antibodies: Mechanism and impact of elevated HIV ELISA false-positive rates in the late-phase COVID-19 pandemic.

This study analyzed the correlation between false-positive HIV ELISA results (using Bio-Rad reagents) and SARS-COV-2 antibody levels in 301 unpaid apheresis platelet donors with prior infection or vaccination, enrolled from Zhejiang Blood Center between February 1 and May 31, 2023. Trends in both the HIV ELISA false-positive rate and SARS-COV-2 antibody levels were assessed. The false-positive rate rose in early 2023, peaking at 0.

Purine metabolism in tumorigenesis and its clinical implications.

Metabolic reprogramming constitutes a hallmark of malignant neoplasms. Purine metabolism emerges as a pivotal regulator in cellular metabolic networks through multiple mechanisms, including dysregulation of de novo biosynthesis/salvage pathway coordination, adenosine-mediated immunosuppressive microenvironment formation, and collective contributions to tumorigenesis and malignant progression. During metastatic progression, purine metabolism reinforces tumor cell plasticity through mitochondrial energy regulation and modulation of cell cycle checkpoints (eg, G1/S transition).

Characteristics of heavy metal element content and health risk assessment in groundwater in Zhaojue area, Sichuan Province, China.

Groundwater serves as the primary drinking water source for residents in the Zhaojue area, located in the hinterland of the Daliang Mountains in Sichuan Province. This study investigated the spatial distribution, pollution characteristics, and health risks of 10 metal elements (As, Cd, Al, Mn, Hg, Co, Cu, Pb, Zn, Ni) through 48 groundwater sampling sites. Comprehensive analysis using single-factor and Nemerow composite pollution index evaluation methods, coupled with multivariate statistics and health risk models, revealed critical findings.

Knowledge, attitude and practice of primary care ophthalmologists towards fundus examination during the perioperative period of cataract surgery in patients with diabetes mellitus: a mediation analysis.

Objectives: This study aimed to investigate the knowledge, attitude and practice (KAP) of primary care ophthalmologists regarding fundus examination during the perioperative period of cataract surgery in patients with diabetes mellitus.

Design: A cross-sectional study was conducted.

Setting: The study took place in hospitals in Shandong Province capable of performing cataract surgeries between June 2023 and January 2024.

Candy box technique with sutures and Nice knot : a novel approach to inferior pole patellar fractures.

Aims: Our study explores the candy box (CB) technique with sutures and Nice knot as a novel treatment for inferior pole patellar fractures, potentially superior to traditional wire fixation.

Methods: CT data from five adult knee joints were extracted to create finite element models for inferior pole patellar fractures and four internal fixation models. These included CB technique combined with high-strength sutures and Nice knot (CB-H), CB technique combined with tendon sutures and Nice knot (CB-T), CB technique combined with steel wires (CB-S), and tension-band wiring combined with cerclage wiring (TBWC).

PSMA PET/CT for Improved Staging Accuracy and Imaging of Neovascularization-associated Features in Primary Lung Cancer.

Background: Prostate-specific membrane antigen (PSMA), expressed in neovascular endothelial cells of various malignancies including lung cancer (LC), highlights its potential as a biomarker for neovascularization. This study aimed to investigate the diagnostic efficacy of PSMA PET/CT in primary lung cancer (PLC), as well as to explore its role in staging and neovascularization detection in PLC.

Patients And Methods: This retrospective study included 39 patients (27 with PLC, 12 with benign lesions) who underwent PSMA PET/CT, with or without FDG PET/CT, between April 2021 and July 2024.

Highly Stable Electrosynthesis of Hydrogen Peroxide Adapted to Fluctuating Renewable Energy.

The integration of renewable energy with electrocatalytic technology affords an effective pathway to reduce carbon emissions and enhance energy efficiency, thereby promoting the green electrification of the chemical industry. However, the inherent contradiction between the fluctuating nature of renewable energy and the need for stable operation in electrochemical processes significantly hinders their development and implementation. To mitigate this, we propose a robust two-electron oxygen reduction reaction system using O-coordinated Co single-atom catalysts for the electrosynthesis of environmentally friendly hydrogen peroxide.

n-Type thermoelectric elastomers.

Intrinsically elastic thermoelectric generators with superior conformal coverage and shape adaptability are highly desirable for developing self-powered wearable electronics, soft bioelectronics and personal temperature regulators. Until now, all reported high-performance thermoelectric materials have realized only flexibility, rather than elasticity. Here we present one of the first n-type thermoelectric elastomers by integrating uniform bulk nanophase separation, thermally activated crosslinking and targeted doping into a single material.

Overcoming immunotherapy resistance in bladder cancer with a novel antibody-drug conjugate RC48.

Background: Immune checkpoint inhibitors have shown limited response rates in bladder cancer. RC48-antibody-drug conjugate (ADC) shows potential for combination with immune checkpoint inhibitors. This study aimed to elucidate RC48-ADC's mechanism in sensitizing tumors to immunotherapy and identify optimal combination strategies.

Strength Enhancement of Clay Through Lime-Sand Stabilization at Various Remolding Water Contents.

During the construction of subgrade, the remolding water content of lime-sand-stabilized clay usually varies in a wide range, leading to inconsistent effectiveness in strength enhancement. Until now, this aspect has not been investigated. In this study, an unconfined compression test and microscopic observation were carried out on clay and stabilized clay (adding 4% lime by mass and 50% sand by volume).

HPLC analysis of gingerols and shogaols discriminated from ginger extracts with a diode array detector.

A simple and inexpensive high performance liquid chromatography (HPLC) method for the analysis of gingerols and shogaols in ginger extracts discriminated with a diode array detector (DAD) has been developed. The ginger extracts were well separated with a linear gradient elution from mixed solvents of water and methanol to methanol within 30 min by using different C18 columns. The separated components were detected with a DAD.

PAICS-Driven Purine Biosynthesis and Its Prognostic Implications in Lung Adenocarcinoma: A Novel Risk Stratification Model and Therapeutic Insights.

Background: Lung adenocarcinoma is the most common NSCLC and is associated with metabolic dysregulation. Purine biosynthesis, regulated by PAICS, plays a key role in tumor progression and therapy resistance.

Methods: We focused on LUAD using pan-cancer and KEGG enrichment analyses.

Apoferritin Nanoparticle-Based Mass Tags: A Novel Metal Tagging Strategy for Mass Cytometry.

Mass cytometry (MC), considered the next generation of flow cytometry (FC), uses antibodies tagged with metal isotopes instead of fluorescent molecules for higher-dimensional single cell biomarker assays and can measure more than 50 parameters simultaneously on individual cells. Despite its powerful analytical performance, MC also has limitations in sensitivity and isotope channels using current mass tags. Herein, a new metal tagging strategy was developed to prepare MC mass tags based on apoferritin nanoparticles (AFNPs).

Deciphering the circadian rhythm in colorectal cancer: a bibliometric analysis of research landscape and trends.

Introduction: Colorectal cancer (CRC) is a leading cause of global cancer mortality, increasingly linked to circadian rhythm disruption-a critical yet underexplored driver of tumorigenesis.

Methods: This bibliometric analysis evaluates 374 publications from the Web of Science Core Collection (1999-2024) using VOSviewer, CiteSpace, and Bibliometrix to map global research trends.

Results: Annual publications surged post-2016, peaking in 2021, reflecting intensified focus on circadian-CRC interactions.

Fabrication of novel biomimetic nano-hydroxyapatite/chitosan injectable hydrogel with chikusetusaponin IVa on chondral injury in osteoarthritis.

The management of pain and the chondral injury resulting from osteoarthritis (OA) present serious challenges in clinical treatment. The conventional method of injecting hyaluronic acid (HA) directly into the joint can alleviate the condition, but it is constrained by the challenge of preserving its effectiveness over an extended time. Recent studies indicate that the total Chikusetusaponin IVa (ChS IVa) found in the Panax japonicas have the ability to reduce oxidative stress and inflammation.

The value of intratumoral and peritumoral ultrasound radiomics model constructed using multiple machine learning algorithms for non-mass breast cancer.

To investigate the diagnostic capability of multiple machine learning algorithms combined with intratumoral and peritumoral ultrasound radiomics models for non-massive breast cancer in dense breast backgrounds. Manual segmentation of ultrasound images was performed to define the intratumoral region of interest (ROI), and five peritumoral ROIs were generated by extending the contours by 1 to 5 mm. A total of 851 radiomics features were extracted from these regions and filtered using statistical methods.

Efficacy and biomarker analysis of neoadjuvant disitamab vedotin (RC48-ADC) combined immunotherapy in patients with muscle-invasive bladder cancer: A multi-center real-world study.

In this study, 102 cisplatin-ineligible patients with muscle-invasive bladder cancer (MIBC) who received neoadjuvant RC48-ADC combined with immunotherapy were included. We evaluated the pathological responses and explored multiple clinical characteristics to identify independent predictive indicators of the efficacy. The results showed that neoadjuvant RC48-ADC combined with immunotherapy had promising efficacy.

Nucleotide-Based Elemental Mass Probes for High-Sensitive Single-Cell Mass Cytometry.

Mass cytometry is one of the most powerful strategies for multiplexed cell-surface protein profiling and cell typing. However, its potential in cell classification is constrained by the lack of elemental mass probes. In this work, we constructed a novel nucleotide-based elemental mass probe.

Matrix stiffness enhances viability, migration, invasion and invadopodia formation of oral cancer cells via PI3K/AKT pathway in vitro.

Background: Oral cancer (OC) is one of the major types of cancer and the most common cause of cancer-related mortality in Asia. In recent years, matrix stiffness in the tumor microenvironment has been found to play an important role in regulating tumor cell behavior. However, the regulatory mechanisms associated with matrix stiffness in OC cells remain unclear.

Single-Cell and Spatial Transcriptomics Explore Purine Metabolism-Related Prognostic Risk Model and Tumor Immune Microenvironment Modulation in Ovarian Cancer.

Ovarian cancer (OC) ranks as the second leading cause of gynecological cancer-related deaths in women globally. Single-cell and spatial transcriptomics could precisely describe the heterogeneity of OC that affect the clinical treatment. Single-cell sequencing and spatial transcriptomics information were from different public datasets.

Application of high-precision 3D laser scanning in the kinematics of the knee joint post total knee arthroplasty.

BackgroundThe number of patients with knee osteoarthritis in China is gradually growing due to the aging society.ObjectiveTo examine the accuracy of two-dimensional and three-dimensional image matching technology equipped with a high-precision 3D laser scanner in kinematics measurement after knee arthroplasty.MethodThe artificial knee joint model fitted with a knee prosthesis was positioned beneath a single-plane X-ray fluoroscope, and the lateral contour of the prosthesis was retrieved through the lateral radiograph of the knee prosthesis.

Total mercury accumulation in Antarctic krill (Euphausia superba) in the northern Antarctic Peninsula during late autumn to early winter.

Antarctic krill (Euphausia superba) is a key species in the Antarctic marine ecosystem and also the largest fishery in terms of catch in the Southern Ocean. Bransfield Strait on the northern Antarctic Peninsula is a key fishing ground. Mercury is a globally recognized toxic element that can affect pelagic organisms through bioaccumulation and biomagnification.

Comparative study of ginsenoside Rg2, 20(S)-protopanaxatriol, and AFG from ginseng on aging-related kidney injury in SAMP8 mice.

Ethnopharmacological Relevance: Aging contributes to various pathologies, including kidney injury, but the therapeutic potential of natural drugs in these contexts remains inadequately assessed. The roots of Panax ginseng C.A.

Multi-omics analysis reveals the mechanism for galactose metabolism in mutant IMAU20551Y.

() is a species widely used in the dairy industry to accelerate the acidification rate and improve the texture and flavour characteristics of dairy products. However, most have galactose-negative (Gal) phenotypes, which can lead to accumulation of free galactose in fermented dairy products. In a previous study, a mutant of IMAU20551Y was obtained by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis in which key enzymes related to galactose metabolism were significantly changed compared with the wild type.

HIF-3α/PPAR-γ Regulates Hypoxia Tolerance by Altering Glycolysis and Lipid Synthesis in Blunt Snout Bream ().

Hypoxic stress causes cell damage and serious diseases in organisms, especially in aquatic animals. It is important to elucidate the changes in metabolic function caused by hypoxia and the mechanisms underlying these changes. This study focuses on the low oxygen tolerance feature of a new blunt snout bream strain (GBSBF1).